(Circular) (Six-base) MAPSORT of: pFN21AB8515.seq Check: 1088 from: 1 to: 7549 pFN21AB8515 7549 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2387 2387 Size: 7549 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3239 4725 5359 278 Size: 53 83 186 936 1703 1486 634 2468 Fragments arranged by size: 2468 1703 1486 936 634 186 83 53 AccI GT'mk_AC Cuts at: 1937 4233 4892 1937 Size: 2296 659 4594 Fragments arranged by size: 4594 2296 659 Acc65I G'GTAC_C Cuts at: 4870 4870 Size: 7549 AclI AA'CG_TT Cuts at: 1563 5676 6049 1563 Size: 4113 373 3063 Fragments arranged by size: 4113 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 2198 2459 2905 3383 3980 4265 4627 Size: 560 6 261 446 478 597 285 362 Cuts at: 4627 5612 6996 7264 1632 Size: 985 1384 268 1917 Fragments arranged by size: 1917 1384 985 597 560 478 446 362 285 268 261 6 AfeI AGC'GCT Cuts at: 2743 2743 Size: 7549 AflII C'TTAA_G Cuts at: 820 1017 3206 820 Size: 197 2189 5163 Fragments arranged by size: 5163 2189 197 AflIII A'CryG_T Cuts at: 1203 4092 4152 4389 6448 7425 1203 Size: 2889 60 237 2059 977 1327 Fragments arranged by size: 2889 2059 1327 977 237 60 AgeI A'CCGG_T Cuts at: 6380 6380 Size: 7549 AhdI GACnn_n'nnGTC Cuts at: 6278 6278 Size: 7549 AleI CACnn'nnGTG Cuts at: 1166 3224 3566 1166 Size: 2058 342 5149 Fragments arranged by size: 5149 2058 342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7549 AlwNI CAG_nnn'CTG Cuts at: 2038 2125 2293 2302 2812 5292 6864 2038 Size: 87 168 9 510 2480 1572 2723 Fragments arranged by size: 2723 2480 1572 510 168 87 9 ApaI G_GGCC'C Cuts at: 2237 2943 2237 Size: 706 6843 ApaLI G'TGCA_C Cuts at: 5606 6762 5606 Size: 1156 6393 ApoI r'AATT_y Cuts at: 1493 3356 3498 3920 4858 5084 5236 7345 1493 Size: 1863 142 422 938 226 152 2109 1697 Fragments arranged by size: 2109 1863 1697 938 422 226 152 142 AseI AT'TA_AT Cuts at: 160 1032 6103 160 Size: 872 5071 1606 Fragments arranged by size: 5071 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7549 AvaI C'yCGr_G Cuts at: 1870 1943 2108 3117 3782 3981 4874 1870 Size: 73 165 1009 665 199 893 4545 Fragments arranged by size: 4545 1009 893 665 199 165 73 BamHI G'GATC_C Cuts at: 3638 3727 4879 5246 3638 Size: 89 1152 367 5941 Fragments arranged by size: 5941 1152 367 89 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2679 2714 3611 4677 4870 Size: 325 217 627 892 35 897 1066 193 Cuts at: 4870 6325 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1066 897 892 627 325 217 193 35 BanII G_rGCy'C Cuts at: 1391 2237 2261 2898 2943 3722 3758 4167 4772 Size: 846 24 637 45 779 36 409 605 Cuts at: 4772 4868 1391 Size: 96 4072 Fragments arranged by size: 4072 846 779 637 605 409 96 45 36 24 BbeI G_GCGC'C Cuts at: 4681 4681 Size: 7549 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4483 928 Size: 971 2584 3994 Fragments arranged by size: 3994 2584 971 BbvCI CC'TCA_GC Cuts at: 3438 3438 Size: 7549 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4595 4629 5740 5774 1903 Size: 34 2658 34 1111 34 3678 Fragments arranged by size: 3678 2658 1111 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5441 6651 5441 Size: 1210 6339 BclI T'GATC_A Cuts at: 1548 1548 Size: 7549 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3150 3953 4670 Size: 107 122 71 631 605 1478 803 717 Cuts at: 4670 6160 136 Size: 1490 1525 Fragments arranged by size: 1525 1490 1478 803 717 631 605 122 107 71 BglII A'GATC_T Cuts at: 7544 7544 Size: 7549 BlpI GC'TnA_GC Cuts at: 2738 4966 2738 Size: 2228 5321 Bme1580I G_kGCm'C Cuts at: 1790 2237 2943 3301 3352 3608 4701 5610 6766 Size: 447 706 358 51 256 1093 909 1156 Cuts at: 6766 1790 Size: 2573 Fragments arranged by size: 2573 1156 1093 909 706 447 358 256 51 BmgBI CAC'GTC Cuts at: 1318 4153 4392 1318 Size: 2835 239 4475 Fragments arranged by size: 4475 2835 239 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2347 3924 4433 Size: 416 53 83 399 24 930 1577 509 Cuts at: 4433 5280 6238 442 Size: 847 958 1753 Fragments arranged by size: 1753 1577 958 930 847 509 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7549 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2067 2912 3297 3492 2067 Size: 845 385 195 6124 Fragments arranged by size: 6124 845 385 195 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2147 2153 2435 2621 2855 3020 3305 Size: 821 87 6 282 186 234 165 285 Cuts at: 3305 3329 3618 6209 1239 Size: 24 289 2591 2579 Fragments arranged by size: 2591 2579 821 289 285 282 234 186 165 87 24 6 Bpu10I CC'TnA_GC Cuts at: 2396 3104 3438 3752 2396 Size: 708 334 314 6193 Fragments arranged by size: 6193 708 334 314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5674 6539 6837 5674 Size: 865 298 6386 Fragments arranged by size: 6386 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3018 3029 3791 3800 6212 882 Size: 617 1519 11 762 9 2412 2219 Fragments arranged by size: 2412 2219 1519 762 617 11 9 BsaAI yAC'GTr Cuts at: 493 1204 2314 3564 4093 5340 493 Size: 711 1110 1250 529 1247 2702 Fragments arranged by size: 2702 1250 1247 1110 711 529 BsaBI GATnn'nnATC Cuts at: 2555 3548 7543 2555 Size: 993 3995 2561 Fragments arranged by size: 3995 2561 993 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3236 4678 4722 5356 Size: 53 83 186 936 1703 1442 44 634 Cuts at: 5356 5738 6373 275 Size: 382 635 1451 Fragments arranged by size: 1703 1451 1442 936 635 634 382 186 83 53 44 BsaWI w'CCGG_w Cuts at: 2499 3579 4084 4332 5982 6380 6654 6801 7212 Size: 1080 505 248 1650 398 274 147 411 Cuts at: 7212 2499 Size: 2836 Fragments arranged by size: 2836 1650 1080 505 411 398 274 248 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2438 4079 4109 1178 Size: 30 165 30 1005 30 1641 30 4618 Fragments arranged by size: 4618 1641 1005 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2251 2899 3902 4496 4499 4754 1186 Size: 1065 648 1003 594 3 255 3981 Fragments arranged by size: 3981 1065 1003 648 594 255 3 BseYI C'CCAG_C Cuts at: 2077 2520 2812 3112 3883 4198 4594 4656 6752 Size: 443 292 300 771 315 396 62 2096 Cuts at: 6752 7248 2077 Size: 496 2378 Fragments arranged by size: 2378 2096 771 496 443 396 315 300 292 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2267 2288 3422 3847 4121 1163 Size: 573 531 21 1134 425 274 4591 Fragments arranged by size: 4591 1134 573 531 425 274 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4119 4542 4714 4999 5760 6788 Size: 275 186 2118 423 172 285 761 1028 Cuts at: 6788 1540 Size: 2301 Fragments arranged by size: 2301 2118 1028 761 423 285 275 186 172 BsiHKAI G_wGCw'C Cuts at: 2700 2898 3722 3758 3912 4092 5610 5695 6766 Size: 198 824 36 154 180 1518 85 1071 Cuts at: 6766 2700 Size: 3483 Fragments arranged by size: 3483 1518 1071 824 198 180 154 85 36 BsmI GAATG_Cn' Cuts at: 5060 5153 5060 Size: 93 7456 BsmBI CGTCTCn'nnnn_ Cuts at: 3978 3978 Size: 7549 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2261 2700 2898 2943 3301 3352 Size: 399 447 24 439 198 45 358 51 Cuts at: 3352 3608 3722 3758 3912 4092 4167 4701 4772 Size: 256 114 36 154 180 75 534 71 Cuts at: 4772 4868 5610 5695 6766 1391 Size: 96 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 534 447 439 399 358 256 198 180 154 114 96 85 75 71 51 45 36 24 BspEI T'CCGG_A Cuts at: 3579 7212 3579 Size: 3633 3916 BspHI T'CATG_A Cuts at: 4557 5438 7168 4557 Size: 881 1730 4938 Fragments arranged by size: 4938 1730 881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2205 2286 2387 4904 844 Size: 1361 81 101 2517 3489 Fragments arranged by size: 3489 2517 1361 101 81 BsrBI CCG'CTC Cuts at: 1394 1823 2137 2143 2839 2986 4588 5436 1394 Size: 429 314 6 696 147 1602 848 3507 Fragments arranged by size: 3507 1602 848 696 429 314 147 6 BsrDI GCAATG_nn' Cuts at: 65 1248 2488 6044 6218 65 Size: 1183 1240 3556 174 1396 Fragments arranged by size: 3556 1396 1240 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2591 4118 6193 6380 1700 Size: 110 781 1527 2075 187 2869 Fragments arranged by size: 2869 2075 1527 781 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7549 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7549 BssSI C'ACGA_G Cuts at: 5609 6621 5609 Size: 1012 6537 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 2530 2970 3400 794 Size: 1508 228 440 430 4943 Fragments arranged by size: 4943 1508 440 430 228 BstBI TT'CG_AA Cuts at: 6353 6353 Size: 7549 BstEII G'GTnAC_C Cuts at: 1184 3442 1184 Size: 2258 5291 BstXI CCAn_nnnn'nTGG Cuts at: 2218 2360 2218 Size: 142 7407 BstYI r'GATC_y Cuts at: 1972 3638 3727 4598 4879 5246 5631 5648 7100 Size: 1666 89 871 281 367 385 17 1452 Cuts at: 7100 7186 7198 7544 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1666 1452 871 385 367 346 281 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4374 4374 Size: 7549 BtgI C'CryG_G Cuts at: 513 1065 2004 3044 3600 3654 4800 513 Size: 552 939 1040 556 54 1146 3262 Fragments arranged by size: 3262 1146 1040 939 556 552 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6810 Fragments arranged by size: 6810 683 56 BtsI GCAGTG_nn' Cuts at: 3774 4035 5065 5859 5879 3774 Size: 261 1030 794 20 5444 Fragments arranged by size: 5444 1030 794 261 20 ClaI AT'CG_AT Cuts at: 1554 3552 1554 Size: 1998 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7514 DraI TTT'AAA Cuts at: 4853 4853 Size: 7549 DraIII CAC_nnn'GTG Cuts at: 2317 2704 2970 2317 Size: 387 266 6896 Fragments arranged by size: 6896 387 266 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4390 4502 6556 809 Size: 507 3074 112 2054 1802 Fragments arranged by size: 3074 2054 1802 507 112 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2334 2385 Size: 54 1425 50 275 18 255 249 51 Cuts at: 2385 2427 2593 2706 2934 3151 4116 4539 4711 Size: 42 166 113 228 217 965 423 172 Cuts at: 4711 4836 4996 5885 7514 8 Size: 125 160 889 1629 43 Fragments arranged by size: 1629 1425 965 889 423 275 255 249 228 217 172 166 160 125 113 54 51 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 4116 4539 4711 4996 1537 Size: 275 2304 423 172 285 4090 Fragments arranged by size: 4090 2304 423 285 275 172 EarI CTCTTCn'nnn_ Cuts at: 1353 2664 2886 3710 4070 5479 1353 Size: 1311 222 824 360 1409 3423 Fragments arranged by size: 3423 1409 1311 824 360 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2471 2564 4174 6136 6510 Size: 1064 138 435 447 93 1610 1962 374 Cuts at: 6510 6656 387 Size: 146 1280 Fragments arranged by size: 1962 1610 1280 1064 447 435 374 146 138 93 EcoICRI GAG'CTC Cuts at: 2896 3720 3756 2896 Size: 824 36 6689 Fragments arranged by size: 6689 824 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2147 2153 2192 2198 2435 2459 Size: 393 428 87 6 39 6 237 24 Cuts at: 2459 2621 2855 2905 3020 3305 3329 3383 3618 Size: 162 234 50 115 285 24 54 235 Cuts at: 3618 3980 4265 4627 5612 6209 6996 7264 1239 Size: 362 285 362 985 597 787 268 1524 Fragments arranged by size: 1524 985 787 597 428 393 362 362 285 285 268 237 235 234 162 115 87 54 50 39 24 24 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 4307 4642 4307 Size: 335 7214 EcoO109I rG'GnC_Cy Cuts at: 2234 2752 2940 2994 3071 3078 3121 3340 3815 Size: 518 188 54 77 7 43 219 475 Cuts at: 3815 4126 4240 4353 4756 2234 Size: 311 114 113 403 5027 Fragments arranged by size: 5027 518 475 403 311 219 188 114 113 77 54 43 7 EcoRI G'AATT_C Cuts at: 3920 4858 5236 3920 Size: 938 378 6233 Fragments arranged by size: 6233 938 378 FseI GG_CCGG'CC Cuts at: 2595 2595 Size: 7549 FspI TGC'GCA Cuts at: 2663 6055 2663 Size: 3392 4157 HaeII r_GCGC'y Cuts at: 1678 2585 2745 4681 6696 1678 Size: 907 160 1936 2015 2531 Fragments arranged by size: 2531 2015 1936 907 160 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4142 4174 6190 6222 6264 Size: 32 135 32 2570 32 2016 32 42 Cuts at: 6264 6296 1373 Size: 32 2626 Fragments arranged by size: 2626 2570 2016 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4507 4893 5139 669 Size: 988 281 2569 386 246 3079 Fragments arranged by size: 3079 2569 988 386 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7549 HpaI GTT'AAC Cuts at: 5139 5139 Size: 7549 KasI G'GCGC_C Cuts at: 4677 4677 Size: 7549 KpnI G_GTAC'C Cuts at: 4874 4874 Size: 7549 MfeI C'AATT_G Cuts at: 5148 5148 Size: 7549 MluI A'CGCG_T Cuts at: 7425 7425 Size: 7549 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2826 3348 3508 4230 6663 6847 1305 Size: 1521 522 160 722 2433 184 2007 Fragments arranged by size: 2433 2007 1521 722 522 184 160 MscI TGG'CCA Cuts at: 10 64 1489 1832 2429 10 Size: 54 1425 343 597 5130 Fragments arranged by size: 5130 1425 597 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2564 3224 3566 3668 5507 5866 6025 Size: 648 1398 660 342 102 1839 359 159 Cuts at: 6025 518 Size: 2042 Fragments arranged by size: 2042 1839 1398 660 648 359 342 159 102 MspA1I CmG'CkG Cuts at: 1606 2058 2077 2125 2293 2599 2915 3337 3406 Size: 452 19 48 168 306 316 422 69 Cuts at: 3406 4802 4965 5642 6790 7035 1606 Size: 1396 163 677 1148 245 2120 Fragments arranged by size: 2120 1396 1148 677 452 422 316 306 245 168 163 69 48 19 NaeI GCC'GGC Cuts at: 2593 2593 Size: 7549 NarI GG'CG_CC Cuts at: 4678 4678 Size: 7549 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6058 Fragments arranged by size: 6058 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7549 NgoMIV G'CCGG_C Cuts at: 2591 2591 Size: 7549 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7549 NotI GC'GGCC_GC Cuts at: 4996 4996 Size: 7549 NspI r_CATG'y Cuts at: 1132 1229 2569 4907 6452 1132 Size: 97 1340 2338 1545 2229 Fragments arranged by size: 2338 2229 1545 1340 97 PasI CC'CwG_GG Cuts at: 1348 3421 3623 4469 1348 Size: 2073 202 846 4428 Fragments arranged by size: 4428 2073 846 202 PciI A'CATG_T Cuts at: 6448 6448 Size: 7549 PflMI CCAn_nnn'nTGG Cuts at: 1108 2812 3045 3264 1108 Size: 1704 233 219 5393 Fragments arranged by size: 5393 1704 233 219 PfoI T'CCnGG_A Cuts at: 3634 3765 4263 4818 3634 Size: 131 498 555 6365 Fragments arranged by size: 6365 555 498 131 PmeI GTTT'AAAC Cuts at: 4853 4853 Size: 7549 PmlI CAC'GTG Cuts at: 2314 3564 4093 2314 Size: 1250 529 5770 Fragments arranged by size: 5770 1250 529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5618 5650 7131 7163 5618 Size: 32 1481 32 6004 Fragments arranged by size: 6004 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2994 3071 3078 4126 4240 4353 2994 Size: 77 7 1048 114 113 6190 Fragments arranged by size: 6190 1048 114 113 77 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7549 PsiI TTA'TAA Cuts at: 5119 5119 Size: 7549 PspOMI G'GGCC_C Cuts at: 2233 2939 2233 Size: 706 6843 PstI C_TGCA'G Cuts at: 830 2298 2589 3023 4901 830 Size: 1468 291 434 1878 3478 Fragments arranged by size: 3478 1878 1468 434 291 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7549 PvuII CAG'CTG Cuts at: 2058 2077 2125 2293 2599 3406 2058 Size: 19 48 168 306 807 6201 Fragments arranged by size: 6201 807 306 168 48 19 RsrII CG'GwC_CG Cuts at: 2758 2758 Size: 7549 SacI G_AGCT'C Cuts at: 2898 3722 3758 2898 Size: 824 36 6689 Fragments arranged by size: 6689 824 36 SacII CC_GC'GG Cuts at: 4803 4803 Size: 7549 SalI G'TCGA_C Cuts at: 1936 4891 1936 Size: 2955 4594 SanDI GG'GwC_CC Cuts at: 2994 3078 4353 2994 Size: 84 1275 6190 Fragments arranged by size: 6190 1275 84 SapI GCTCTTCn'nnn_ Cuts at: 2664 2886 3710 2664 Size: 222 824 6503 Fragments arranged by size: 6503 824 222 SbfI CC_TGCA'GG Cuts at: 4901 4901 Size: 7549 ScaI AGT'ACT Cuts at: 4112 5797 7509 4112 Size: 1685 1712 4152 Fragments arranged by size: 4152 1712 1685 SfcI C'TryA_G Cuts at: 826 1045 2294 2476 2585 2959 3019 4205 4234 Size: 219 1249 182 109 374 60 1186 29 Cuts at: 4234 4897 6032 6713 6904 826 Size: 663 1135 681 191 1471 Fragments arranged by size: 1471 1249 1186 1135 681 663 374 219 191 182 109 60 29 SfoI GGC'GCC Cuts at: 4679 4679 Size: 7549 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7549 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7549 SmaI CCC'GGG Cuts at: 1872 2110 3119 4876 1872 Size: 238 1009 1757 4545 Fragments arranged by size: 4545 1757 1009 238 SmlI C'TyrA_G Cuts at: 820 1017 1943 3206 5653 6554 6816 820 Size: 197 926 1263 2447 901 262 1553 Fragments arranged by size: 2447 1553 1263 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5340 493 Size: 4847 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7549 SphI G_CATG'C Cuts at: 1132 2569 4907 1132 Size: 1437 2338 3774 Fragments arranged by size: 3774 2338 1437 SspI AAT'ATT Cuts at: 5 52 5473 5 Size: 47 5421 2081 Fragments arranged by size: 5421 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7549 StyI C'CwwG_G Cuts at: 513 1065 2004 2493 2629 2748 2871 4988 513 Size: 552 939 489 136 119 123 2117 3074 Fragments arranged by size: 3074 2117 939 552 489 136 123 119 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 2642 3556 4415 5576 5593 5746 Size: 1255 774 288 914 859 1161 17 153 Cuts at: 5746 5931 6376 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1161 914 859 774 445 288 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4110 5795 7507 Size: 274 80 33 51 1443 2133 1685 1712 Cuts at: 7507 96 Size: 138 Fragments arranged by size: 2133 1712 1685 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3235 3267 3643 3675 4079 4111 4413 4445 7509 Size: 32 376 32 404 32 302 32 3064 Cuts at: 7509 7541 3235 Size: 32 3243 Fragments arranged by size: 3243 3064 404 376 302 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7549 XbaI T'CTAG_A Cuts at: 4885 4885 Size: 7549 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2215 2809 2215 Size: 594 6955 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7549 XmaI C'CCGG_G Cuts at: 1870 2108 3117 4874 1870 Size: 238 1009 1757 4545 Fragments arranged by size: 4545 1757 1009 238 XmnI GAAnn'nnTTC Cuts at: 3500 3524 4064 5678 3500 Size: 24 540 1614 5371 Fragments arranged by size: 5371 1614 540 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3237 4723 5357 276 Size: 53 83 186 936 1703 1486 634 2468 Fragments arranged by size: 2468 1703 1486 936 634 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI SexAI SfiI SrfI SwaI