Restriction map for
pFN21AB8515
(Linear) (Six-base) MAPSORT of: KIBB8515.seq Check: 4749 from: 1 to: 2844
KIBB8515.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 382 2844
Size: 382 2462
AatII G_ACGT'C
Cuts at: 0 1234 2720 2844
Size: 1234 1486 124
Fragments arranged by size:
1486 1234 124
AccI GT'mk_AC
Cuts at: 0 2228 2844
Size: 2228 616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 193 454 900 1378 1975 2260 2622
Size: 187 6 261 446 478 597 285 362
Cuts at: 2622 2844
Size: 222
Fragments arranged by size:
597 478 446 362 285 261 222 187
6
AfeI AGC'GCT
Cuts at: 0 738 2844
Size: 738 2106
AflII C'TTAA_G
Cuts at: 0 1201 2844
Size: 1201 1643
AflIII A'CryG_T
Cuts at: 0 2087 2147 2384 2844
Size: 2087 60 237 460
Fragments arranged by size:
2087 460 237 60
AleI CACnn'nnGTG
Cuts at: 0 1219 1561 2844
Size: 1219 342 1283
Fragments arranged by size:
1283 1219 342
AlwNI CAG_nnn'CTG
Cuts at: 0 33 120 288 297 807 2844
Size: 33 87 168 9 510 2037
Fragments arranged by size:
2037 510 168 87 33 9
ApaI G_GGCC'C
Cuts at: 0 232 938 2844
Size: 232 706 1906
Fragments arranged by size:
1906 706 232
ApoI r'AATT_y
Cuts at: 0 1351 1493 1915 2844
Size: 1351 142 422 929
Fragments arranged by size:
1351 929 422 142
AvaI C'yCGr_G
Cuts at: 0 103 1112 1777 1976 2844
Size: 103 1009 665 199 868
Fragments arranged by size:
1009 868 665 199 103
BamHI G'GATC_C
Cuts at: 0 1633 1722 2844
Size: 1633 89 1122
Fragments arranged by size:
1633 1122 89
BanI G'GyrC_C
Cuts at: 0 674 709 1606 2672 2844
Size: 674 35 897 1066 172
Fragments arranged by size:
1066 897 674 172 35
BanII G_rGCy'C
Cuts at: 0 232 256 893 938 1717 1753 2162 2767
Size: 232 24 637 45 779 36 409 605
Cuts at: 2767 2844
Size: 77
Fragments arranged by size:
779 637 605 409 232 77 45 36
24
BbeI G_GCGC'C
Cuts at: 0 2676 2844
Size: 2676 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 2478 2844
Size: 2478 366
BbvCI CC'TCA_GC
Cuts at: 0 1433 2844
Size: 1433 1411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2590 2624 2844
Size: 2590 34 220
Fragments arranged by size:
2590 220 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1145 1948 2665 2844
Size: 1145 803 717 179
Fragments arranged by size:
1145 803 717 179
BlpI GC'TnA_GC
Cuts at: 0 733 2844
Size: 733 2111
Bme1580I G_kGCm'C
Cuts at: 0 232 938 1296 1347 1603 2696 2844
Size: 232 706 358 51 256 1093 148
Fragments arranged by size:
1093 706 358 256 232 148 51
BmgBI CAC'GTC
Cuts at: 0 2148 2387 2844
Size: 2148 239 457
Fragments arranged by size:
2148 457 239
BmrI ACTGGGnnnn_n'
Cuts at: 0 342 1919 2428 2844
Size: 342 1577 509 416
Fragments arranged by size:
1577 509 416 342
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 62 907 1292 1487 2844
Size: 62 845 385 195 1357
Fragments arranged by size:
1357 845 385 195 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 142 148 430 616 850 1015 1300
Size: 55 87 6 282 186 234 165 285
Cuts at: 1300 1324 1613 2844
Size: 24 289 1231
Fragments arranged by size:
1231 289 285 282 234 186 165 87
55 24 6
Bpu10I CC'TnA_GC
Cuts at: 0 391 1099 1433 1747 2844
Size: 391 708 334 314 1097
Fragments arranged by size:
1097 708 391 334 314
BsaI GGTCTCn'nnnn_
Cuts at: 0 1013 1024 1786 1795 2844
Size: 1013 11 762 9 1049
Fragments arranged by size:
1049 1013 762 11 9
BsaAI yAC'GTr
Cuts at: 0 309 1559 2088 2844
Size: 309 1250 529 756
Fragments arranged by size:
1250 756 529 309
BsaBI GATnn'nnATC
Cuts at: 0 550 1543 2844
Size: 550 993 1301
Fragments arranged by size:
1301 993 550
BsaHI Gr'CG_yC
Cuts at: 0 1231 2673 2717 2844
Size: 1231 1442 44 127
Fragments arranged by size:
1442 1231 127 44
BsaWI w'CCGG_w
Cuts at: 0 494 1574 2079 2327 2844
Size: 494 1080 505 248 517
Fragments arranged by size:
1080 517 505 494 248
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 433 2074 2104 2844
Size: 403 30 1641 30 740
Fragments arranged by size:
1641 740 403 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 246 894 1897 2491 2494 2749 2844
Size: 246 648 1003 594 3 255 95
Fragments arranged by size:
1003 648 594 255 246 95 3
BseYI C'CCAG_C
Cuts at: 0 72 515 807 1107 1878 2193 2589 2651
Size: 72 443 292 300 771 315 396 62
Cuts at: 2651 2844
Size: 193
Fragments arranged by size:
771 443 396 315 300 292 193 72
62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 283 1417 1842 2116 2844
Size: 262 21 1134 425 274 728
Fragments arranged by size:
1134 728 425 274 262 21
BsiEI CG_ry'CG
Cuts at: 0 2114 2537 2709 2844
Size: 2114 423 172 135
Fragments arranged by size:
2114 423 172 135
BsiHKAI G_wGCw'C
Cuts at: 0 695 893 1717 1753 1907 2087 2844
Size: 695 198 824 36 154 180 757
Fragments arranged by size:
824 757 695 198 180 154 36
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1973 2844
Size: 1973 871
Bsp1286I G_dGCh'C
Cuts at: 0 232 256 695 893 938 1296 1347 1603
Size: 232 24 439 198 45 358 51 256
Cuts at: 1603 1717 1753 1907 2087 2162 2696 2767 2844
Size: 114 36 154 180 75 534 71 77
Fragments arranged by size:
534 439 358 256 232 198 180 154
114 77 75 71 51 45 36 24
BspEI T'CCGG_A
Cuts at: 0 1574 2844
Size: 1574 1270
BspHI T'CATG_A
Cuts at: 0 2552 2844
Size: 2552 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 200 281 382 2844
Size: 200 81 101 2462
Fragments arranged by size:
2462 200 101 81
BsrBI CCG'CTC
Cuts at: 0 132 138 834 981 2583 2844
Size: 132 6 696 147 1602 261
Fragments arranged by size:
1602 696 261 147 132 6
BsrDI GCAATG_nn'
Cuts at: 0 483 2844
Size: 483 2361
BsrFI r'CCGG_y
Cuts at: 0 586 2113 2844
Size: 586 1527 731
Fragments arranged by size:
1527 731 586
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 525 965 1395 2844
Size: 297 228 440 430 1449
Fragments arranged by size:
1449 440 430 297 228
BstEII G'GTnAC_C
Cuts at: 0 1437 2844
Size: 1437 1407
BstXI CCAn_nnnn'nTGG
Cuts at: 0 213 355 2844
Size: 213 142 2489
Fragments arranged by size:
2489 213 142
BstYI r'GATC_y
Cuts at: 0 1633 1722 2593 2844
Size: 1633 89 871 251
Fragments arranged by size:
1633 871 251 89
Bsu36I CC'TnA_GG
Cuts at: 0 2369 2844
Size: 2369 475
BtgI C'CryG_G
Cuts at: 0 1039 1595 1649 2795 2844
Size: 1039 556 54 1146 49
Fragments arranged by size:
1146 1039 556 54 49
BtsI GCAGTG_nn'
Cuts at: 0 1769 2030 2844
Size: 1769 261 814
Fragments arranged by size:
1769 814 261
ClaI AT'CG_AT
Cuts at: 0 1547 2844
Size: 1547 1297
DraIII CAC_nnn'GTG
Cuts at: 0 312 699 965 2844
Size: 312 387 266 1879
Fragments arranged by size:
1879 387 312 266
DrdI GACnn_nn'nnGTC
Cuts at: 0 2385 2497 2844
Size: 2385 112 347
Fragments arranged by size:
2385 347 112
EaeI y'GGCC_r
Cuts at: 0 80 329 380 422 588 701 929 1146
Size: 80 249 51 42 166 113 228 217
Cuts at: 1146 2111 2534 2706 2831 2844
Size: 965 423 172 125 13
Fragments arranged by size:
965 423 249 228 217 172 166 125
113 80 51 42 13
EagI C'GGCC_G
Cuts at: 0 2111 2534 2706 2844
Size: 2111 423 172 138
Fragments arranged by size:
2111 423 172 138
EarI CTCTTCn'nnn_
Cuts at: 0 659 881 1705 2065 2844
Size: 659 222 824 360 779
Fragments arranged by size:
824 779 659 360 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 466 559 2169 2844
Size: 19 447 93 1610 675
Fragments arranged by size:
1610 675 447 93 19
EcoICRI GAG'CTC
Cuts at: 0 891 1715 1751 2844
Size: 891 824 36 1093
Fragments arranged by size:
1093 891 824 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 142 148 187 193 430 454 616
Size: 55 87 6 39 6 237 24 162
Cuts at: 616 850 900 1015 1300 1324 1378 1613 1975
Size: 234 50 115 285 24 54 235 362
Cuts at: 1975 2260 2622 2844
Size: 285 362 222
Fragments arranged by size:
362 362 285 285 237 235 234 222
162 115 87 55 54 50 39 24
24 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2302 2637 2844
Size: 2302 335 207
Fragments arranged by size:
2302 335 207
EcoO109I rG'GnC_Cy
Cuts at: 0 229 747 935 989 1066 1073 1116 1335
Size: 229 518 188 54 77 7 43 219
Cuts at: 1335 1810 2121 2235 2348 2751 2844
Size: 475 311 114 113 403 93
Fragments arranged by size:
518 475 403 311 229 219 188 114
113 93 77 54 43 7
EcoRI G'AATT_C
Cuts at: 0 1915 2844
Size: 1915 929
FseI GG_CCGG'CC
Cuts at: 0 590 2844
Size: 590 2254
FspI TGC'GCA
Cuts at: 0 658 2844
Size: 658 2186
HaeII r_GCGC'y
Cuts at: 0 580 740 2676 2844
Size: 580 160 1936 168
Fragments arranged by size:
1936 580 168 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2137 2169 2844
Size: 2137 32 675
Fragments arranged by size:
2137 675 32
HincII GTy'rAC
Cuts at: 0 2502 2844
Size: 2502 342
KasI G'GCGC_C
Cuts at: 0 2672 2844
Size: 2672 172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1343 1503 2225 2844
Size: 821 522 160 722 619
Fragments arranged by size:
821 722 619 522 160
MscI TGG'CCA
Cuts at: 0 424 2844
Size: 424 2420
MslI CAynn'nnrTG
Cuts at: 0 559 1219 1561 1663 2844
Size: 559 660 342 102 1181
Fragments arranged by size:
1181 660 559 342 102
MspA1I CmG'CkG
Cuts at: 0 53 72 120 288 594 910 1332 1401
Size: 53 19 48 168 306 316 422 69
Cuts at: 1401 2797 2844
Size: 1396 47
Fragments arranged by size:
1396 422 316 306 168 69 53 48
47 19
NaeI GCC'GGC
Cuts at: 0 588 2844
Size: 588 2256
NarI GG'CG_CC
Cuts at: 0 2673 2844
Size: 2673 171
NgoMIV G'CCGG_C
Cuts at: 0 586 2844
Size: 586 2258
NspI r_CATG'y
Cuts at: 0 564 2844
Size: 564 2280
PasI CC'CwG_GG
Cuts at: 0 1416 1618 2464 2844
Size: 1416 202 846 380
Fragments arranged by size:
1416 846 380 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 807 1040 1259 2844
Size: 807 233 219 1585
Fragments arranged by size:
1585 807 233 219
PfoI T'CCnGG_A
Cuts at: 0 1629 1760 2258 2813 2844
Size: 1629 131 498 555 31
Fragments arranged by size:
1629 555 498 131 31
PmlI CAC'GTG
Cuts at: 0 309 1559 2088 2844
Size: 309 1250 529 756
Fragments arranged by size:
1250 756 529 309
PpuMI rG'GwC_Cy
Cuts at: 0 989 1066 1073 2121 2235 2348 2844
Size: 989 77 7 1048 114 113 496
Fragments arranged by size:
1048 989 496 114 113 77 7
PspOMI G'GGCC_C
Cuts at: 0 228 934 2844
Size: 228 706 1910
Fragments arranged by size:
1910 706 228
PstI C_TGCA'G
Cuts at: 0 293 584 1018 2844
Size: 293 291 434 1826
Fragments arranged by size:
1826 434 293 291
PvuII CAG'CTG
Cuts at: 0 53 72 120 288 594 1401 2844
Size: 53 19 48 168 306 807 1443
Fragments arranged by size:
1443 807 306 168 53 48 19
RsrII CG'GwC_CG
Cuts at: 0 753 2844
Size: 753 2091
SacI G_AGCT'C
Cuts at: 0 893 1717 1753 2844
Size: 893 824 36 1091
Fragments arranged by size:
1091 893 824 36
SacII CC_GC'GG
Cuts at: 0 2798 2844
Size: 2798 46
SanDI GG'GwC_CC
Cuts at: 0 989 1073 2348 2844
Size: 989 84 1275 496
Fragments arranged by size:
1275 989 496 84
SapI GCTCTTCn'nnn_
Cuts at: 0 659 881 1705 2844
Size: 659 222 824 1139
Fragments arranged by size:
1139 824 659 222
ScaI AGT'ACT
Cuts at: 0 2107 2844
Size: 2107 737
SfcI C'TryA_G
Cuts at: 0 289 471 580 954 1014 2200 2229 2844
Size: 289 182 109 374 60 1186 29 615
Fragments arranged by size:
1186 615 374 289 182 109 60 29
SfoI GGC'GCC
Cuts at: 0 2674 2844
Size: 2674 170
SmaI CCC'GGG
Cuts at: 0 105 1114 2844
Size: 105 1009 1730
Fragments arranged by size:
1730 1009 105
SmlI C'TyrA_G
Cuts at: 0 1201 2844
Size: 1201 1643
SphI G_CATG'C
Cuts at: 0 564 2844
Size: 564 2280
StyI C'CwwG_G
Cuts at: 0 488 624 743 866 2844
Size: 488 136 119 123 1978
Fragments arranged by size:
1978 488 136 123 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 349 637 1551 2410 2844
Size: 349 288 914 859 434
Fragments arranged by size:
914 859 434 349 288
TatI w'GTAC_w
Cuts at: 0 2105 2844
Size: 2105 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1230 1262 1638 1670 2074 2106 2408 2440
Size: 1230 32 376 32 404 32 302 32
Cuts at: 2440 2844
Size: 404
Fragments arranged by size:
1230 404 404 376 302 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 210 804 2844
Size: 210 594 2040
Fragments arranged by size:
2040 594 210
XmaI C'CCGG_G
Cuts at: 0 103 1112 2844
Size: 103 1009 1732
Fragments arranged by size:
1732 1009 103
XmnI GAAnn'nnTTC
Cuts at: 0 1495 1519 2059 2844
Size: 1495 24 540 785
Fragments arranged by size:
1495 785 540 24
ZraI GAC'GTC
Cuts at: 0 1232 2718 2844
Size: 1232 1486 126
Fragments arranged by size:
1486 1232 126
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AleI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII RsrII
SacI SacII SanDI SapI ScaI SfcI SfoI SmaI
SmlI SphI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AhdI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII
BmtI BpuEI BsiWI BsmI BsrGI BssHII BssSI BstBI
BstZ17I BtgZI CspCI DraI EcoRV FalI FspAI HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI SalI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XhoI