(Circular) (Six-base) MAPSORT of: pFN21AB8503.seq Check: 4475 from: 1 to: 7516 pFN21AB8503 7516 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5326 278 Size: 53 83 186 936 3790 2468 Fragments arranged by size: 3790 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3018 4637 4859 1937 Size: 1081 1619 222 4594 Fragments arranged by size: 4594 1619 1081 222 Acc65I G'GTAC_C Cuts at: 4837 4837 Size: 7516 AclI AA'CG_TT Cuts at: 1563 5643 6016 1563 Size: 4080 373 3063 Fragments arranged by size: 4080 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3087 3381 3670 3861 3915 4029 4049 5579 Size: 1455 294 289 191 54 114 20 1530 Cuts at: 5579 6963 7231 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1530 1455 1384 294 289 268 191 114 54 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7319 AflIII A'CryG_T Cuts at: 1203 4376 6415 7392 1203 Size: 3173 2039 977 1327 Fragments arranged by size: 3173 2039 1327 977 AgeI A'CCGG_T Cuts at: 6347 6347 Size: 7516 AhdI GACnn_n'nnGTC Cuts at: 6245 6245 Size: 7516 AleI CACnn'nnGTG Cuts at: 1166 4695 1166 Size: 3529 3987 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7516 AlwNI CAG_nnn'CTG Cuts at: 3569 5259 6831 3569 Size: 1690 1572 4254 Fragments arranged by size: 4254 1690 1572 ApaI G_GGCC'C Cuts at: 4503 4503 Size: 7516 ApaLI G'TGCA_C Cuts at: 5573 6729 5573 Size: 1156 6360 ApoI r'AATT_y Cuts at: 1493 2084 2093 2213 2401 2693 2824 2975 3443 Size: 591 9 120 188 292 131 151 468 Cuts at: 3443 3966 4825 5051 5203 7312 1493 Size: 523 859 226 152 2109 1697 Fragments arranged by size: 2109 1697 859 591 523 468 292 226 188 152 151 131 120 9 AseI AT'TA_AT Cuts at: 160 1032 2805 6070 160 Size: 872 1773 3265 1606 Fragments arranged by size: 3265 1773 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 AvaI C'yCGr_G Cuts at: 1870 1943 4841 1870 Size: 73 2898 4545 Fragments arranged by size: 4545 2898 73 BamHI G'GATC_C Cuts at: 4549 4846 5213 4549 Size: 297 367 6852 Fragments arranged by size: 6852 367 297 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4837 6292 618 Size: 325 217 627 3050 1455 1842 Fragments arranged by size: 3050 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4290 4503 4835 1391 Size: 2899 213 332 4072 Fragments arranged by size: 4072 2899 332 213 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3387 4655 928 Size: 971 1488 1268 3789 Fragments arranged by size: 3789 1488 1268 971 BbvCI CC'TCA_GC Cuts at: 2688 2688 Size: 7516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5707 5741 1903 Size: 34 3770 34 3678 Fragments arranged by size: 3770 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2724 5408 6618 2724 Size: 2684 1210 3622 Fragments arranged by size: 3622 2684 1210 BclI T'GATC_A Cuts at: 1548 4387 1548 Size: 2839 4677 BfrBI ATG'CAT Cuts at: 3111 4619 4747 3111 Size: 1508 128 5880 Fragments arranged by size: 5880 1508 128 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6127 136 Size: 107 122 71 631 605 4455 1525 Fragments arranged by size: 4455 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7511 7511 Size: 7516 BlpI GC'TnA_GC Cuts at: 4933 4933 Size: 7516 Bme1580I G_kGCm'C Cuts at: 1790 4257 4503 5577 6733 1790 Size: 2467 246 1074 1156 2573 Fragments arranged by size: 2573 2467 1156 1074 246 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7516 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5247 6205 442 Size: 416 53 83 399 24 3830 958 1753 Fragments arranged by size: 3830 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7516 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2442 2561 3831 3980 4447 6176 1239 Size: 1203 119 1270 149 467 1729 2579 Fragments arranged by size: 2579 1729 1270 1203 467 149 119 Bpu10I CC'TnA_GC Cuts at: 2688 2688 Size: 7516 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3345 4165 4582 4782 5641 6506 6804 3345 Size: 820 417 200 859 865 298 4057 Fragments arranged by size: 4057 865 859 820 417 298 200 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3765 6179 882 Size: 617 2266 2414 2219 Fragments arranged by size: 2414 2266 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5307 493 Size: 711 4103 2702 Fragments arranged by size: 4103 2702 711 BsaBI GATnn'nnATC Cuts at: 7510 7510 Size: 7516 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5323 5705 6340 275 Size: 53 83 186 936 3790 382 635 1451 Fragments arranged by size: 3790 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4552 5949 6347 6621 6768 7179 4552 Size: 1397 398 274 147 411 4889 Fragments arranged by size: 4889 1397 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4420 4450 1178 Size: 30 165 30 3017 30 4244 Fragments arranged by size: 4244 3017 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3491 1186 Size: 2305 5211 BseYI C'CCAG_C Cuts at: 4768 6719 7215 4768 Size: 1951 496 5069 Fragments arranged by size: 5069 1951 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2775 1163 Size: 573 1039 5904 Fragments arranged by size: 5904 1039 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4966 5727 6755 1540 Size: 275 186 2965 761 1028 2301 Fragments arranged by size: 2965 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3103 3405 4692 5577 5662 6733 3103 Size: 302 1287 885 85 1071 3886 Fragments arranged by size: 3886 1287 1071 885 302 85 BsmI GAATG_Cn' Cuts at: 3727 5027 5120 3727 Size: 1300 93 6123 Fragments arranged by size: 6123 1300 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3103 3405 4257 4290 4503 4692 4835 Size: 399 1313 302 852 33 213 189 143 Cuts at: 4835 5577 5662 6733 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1313 1071 852 742 399 302 213 189 143 85 33 BspEI T'CCGG_A Cuts at: 4552 7179 4552 Size: 2627 4889 BspHI T'CATG_A Cuts at: 2209 5405 7135 2209 Size: 3196 1730 2590 Fragments arranged by size: 3196 2590 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4871 844 Size: 4027 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5403 1394 Size: 429 3580 3507 Fragments arranged by size: 3580 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2524 3283 3658 6011 6185 65 Size: 1183 1276 759 375 2353 174 1396 Fragments arranged by size: 2353 1396 1276 1183 759 375 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6160 6347 1700 Size: 110 4350 187 2869 Fragments arranged by size: 4350 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4379 96 Size: 4283 3233 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7516 BssSI C'ACGA_G Cuts at: 5576 6588 5576 Size: 1012 6504 BstAPI GCAn_nnn'nTGC Cuts at: 794 2705 794 Size: 1911 5605 BstBI TT'CG_AA Cuts at: 2023 3972 6320 2023 Size: 1949 2348 3219 Fragments arranged by size: 3219 2348 1949 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7516 BstYI r'GATC_y Cuts at: 1972 4549 4846 5213 5598 5615 7067 7153 7165 Size: 2577 297 367 385 17 1452 86 12 Cuts at: 7165 7511 1972 Size: 346 1977 Fragments arranged by size: 2577 1977 1452 385 367 346 297 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2586 2586 Size: 7516 BtgI C'CryG_G Cuts at: 513 1065 2004 4503 513 Size: 552 939 2499 3526 Fragments arranged by size: 3526 2499 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6777 Fragments arranged by size: 6777 683 56 BtsI GCAGTG_nn' Cuts at: 2280 2571 2960 4441 5032 5826 5846 2280 Size: 291 389 1481 591 794 20 3950 Fragments arranged by size: 3950 1481 794 591 389 291 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7516 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7481 DraI TTT'AAA Cuts at: 2406 2763 4820 2406 Size: 357 2057 5102 Fragments arranged by size: 5102 2057 357 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6523 809 Size: 507 5207 1802 Fragments arranged by size: 5207 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3907 4963 5852 Size: 54 1425 50 275 18 2077 1056 889 Cuts at: 5852 7481 8 Size: 1629 43 Fragments arranged by size: 2077 1629 1425 1056 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4963 1537 Size: 275 3151 4090 Fragments arranged by size: 4090 3151 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2038 2169 3384 4005 4226 4482 4680 5446 Size: 685 131 1215 621 221 256 198 766 Cuts at: 5446 1353 Size: 3423 Fragments arranged by size: 3423 1215 766 685 621 256 221 198 131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6103 6477 6623 387 Size: 1064 138 4514 374 146 1280 Fragments arranged by size: 4514 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2442 2561 3087 3381 3670 3831 3861 Size: 393 810 119 526 294 289 161 30 Cuts at: 3861 3915 3980 4029 4049 4447 5579 6176 6963 Size: 54 65 49 20 398 1132 597 787 Cuts at: 6963 7231 1239 Size: 268 1524 Fragments arranged by size: 1524 1132 810 787 597 526 398 393 294 289 268 161 119 65 54 49 30 20 EcoNI CCTnn'n_nnAGG Cuts at: 2304 2304 Size: 7516 EcoRI G'AATT_C Cuts at: 2213 2824 4825 5203 2213 Size: 611 2001 378 4526 Fragments arranged by size: 4526 2001 611 378 EcoRV GAT'ATC Cuts at: 3283 4126 3283 Size: 843 6673 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3092 3092 Size: 7516 FspI TGC'GCA Cuts at: 6022 6022 Size: 7516 HaeII r_GCGC'y Cuts at: 1678 6663 1678 Size: 4985 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3305 3337 3509 3541 4502 Size: 32 135 32 1733 32 172 32 961 Cuts at: 4502 4534 6157 6189 6231 6263 1373 Size: 32 1623 32 42 32 2626 Fragments arranged by size: 2626 1733 1623 961 172 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4569 4779 4860 5106 669 Size: 988 281 2631 210 81 246 3079 Fragments arranged by size: 3079 2631 988 281 246 210 81 HindIII A'AGCT_T Cuts at: 748 2242 2854 4181 748 Size: 1494 612 1327 4083 Fragments arranged by size: 4083 1494 1327 612 HpaI GTT'AAC Cuts at: 4569 4779 5106 4569 Size: 210 327 6979 Fragments arranged by size: 6979 327 210 KpnI G_GTAC'C Cuts at: 4841 4841 Size: 7516 MfeI C'AATT_G Cuts at: 3539 5115 3539 Size: 1576 5940 MluI A'CGCG_T Cuts at: 7392 7392 Size: 7516 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2416 2550 2981 6630 6814 1305 Size: 1111 134 431 3649 184 2007 Fragments arranged by size: 3649 2007 1111 431 184 134 MscI TGG'CCA Cuts at: 10 64 1489 1832 3909 10 Size: 54 1425 343 2077 3617 Fragments arranged by size: 3617 2077 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 3417 4695 5474 5833 5992 518 Size: 648 858 1393 1278 779 359 159 2042 Fragments arranged by size: 2042 1393 1278 858 779 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3026 3695 4932 5609 6757 7002 1606 Size: 1420 669 1237 677 1148 245 2120 Fragments arranged by size: 2120 1420 1237 1148 677 669 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6025 Fragments arranged by size: 6025 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7516 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7516 NotI GC'GGCC_GC Cuts at: 4963 4963 Size: 7516 NsiI A_TGCA'T Cuts at: 3113 4621 4749 3113 Size: 1508 128 5880 Fragments arranged by size: 5880 1508 128 NspI r_CATG'y Cuts at: 1132 1229 3981 4380 4751 4874 6419 1132 Size: 97 2752 399 371 123 1545 2229 Fragments arranged by size: 2752 2229 1545 399 371 123 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7516 PciI A'CATG_T Cuts at: 4376 6415 4376 Size: 2039 5477 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7516 PfoI T'CCnGG_A Cuts at: 2458 3847 4395 2458 Size: 1389 548 5579 Fragments arranged by size: 5579 1389 548 PmeI GTTT'AAAC Cuts at: 4820 4820 Size: 7516 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5585 5617 7098 7130 5585 Size: 32 1481 32 5971 Fragments arranged by size: 5971 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4633 1613 Size: 3020 4496 PsiI TTA'TAA Cuts at: 5086 5086 Size: 7516 PspOMI G'GGCC_C Cuts at: 4499 4499 Size: 7516 PstI C_TGCA'G Cuts at: 830 4324 4868 830 Size: 3494 544 3478 Fragments arranged by size: 3494 3478 544 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7516 PvuII CAG'CTG Cuts at: 3026 3695 3026 Size: 669 6847 SalI G'TCGA_C Cuts at: 1936 4858 1936 Size: 2922 4594 SapI GCTCTTCn'nnn_ Cuts at: 4680 4680 Size: 7516 SbfI CC_TGCA'GG Cuts at: 4868 4868 Size: 7516 ScaI AGT'ACT Cuts at: 5764 7476 5764 Size: 1712 5804 SexAI A'CCwGG_T Cuts at: 3424 3424 Size: 7516 SfcI C'TryA_G Cuts at: 826 1045 4118 4320 4638 4864 5999 6680 6871 Size: 219 3073 202 318 226 1135 681 191 Cuts at: 6871 826 Size: 1471 Fragments arranged by size: 3073 1471 1135 681 318 226 219 202 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7516 SmaI CCC'GGG Cuts at: 1872 4843 1872 Size: 2971 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3360 4144 4597 4797 5620 6521 Size: 197 926 1417 784 453 200 823 901 Cuts at: 6521 6783 820 Size: 262 1553 Fragments arranged by size: 1553 1417 926 901 823 784 453 262 200 197 SnaBI TAC'GTA Cuts at: 493 5307 493 Size: 4814 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7516 SphI G_CATG'C Cuts at: 1132 4751 4874 1132 Size: 3619 123 3774 Fragments arranged by size: 3774 3619 123 SspI AAT'ATT Cuts at: 5 52 3486 3637 3838 5440 5 Size: 47 3434 151 201 1602 2081 Fragments arranged by size: 3434 2081 1602 201 151 47 StuI AGG'CCT Cuts at: 1517 2952 4076 1517 Size: 1435 1124 4957 Fragments arranged by size: 4957 1435 1124 StyI C'CwwG_G Cuts at: 513 1065 2004 2169 4194 4293 4752 4955 513 Size: 552 939 165 2025 99 459 203 3074 Fragments arranged by size: 3074 2025 939 552 459 203 165 99 SwaI ATTT'AAAT Cuts at: 2406 2406 Size: 7516 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4331 5543 5560 5713 5898 6343 325 Size: 1255 2751 1212 17 153 185 445 1498 Fragments arranged by size: 2751 1498 1255 1212 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2028 4379 5762 Size: 274 80 33 51 1443 51 2351 1383 Cuts at: 5762 7474 96 Size: 1712 138 Fragments arranged by size: 2351 1712 1443 1383 274 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4420 4452 7476 7508 4420 Size: 32 3024 32 4428 Fragments arranged by size: 4428 3024 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7516 XbaI T'CTAG_A Cuts at: 4852 4852 Size: 7516 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2383 4136 2383 Size: 1753 5763 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7516 XmaI C'CCGG_G Cuts at: 1870 4841 1870 Size: 2971 4545 XmnI GAAnn'nnTTC Cuts at: 5645 5645 Size: 7516 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5324 276 Size: 53 83 186 936 3790 2468 Fragments arranged by size: 3790 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI