(Circular) (Six-base) MAPSORT of: pFN21AB8491.seq Check: 1284 from: 1 to: 7414 pFN21AB8491 7414 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3541 3541 Size: 7414 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5224 278 Size: 53 83 186 936 3688 2468 Fragments arranged by size: 3688 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4191 4757 1937 Size: 2254 566 4594 Fragments arranged by size: 4594 2254 566 Acc65I G'GTAC_C Cuts at: 4735 4735 Size: 7414 AclI AA'CG_TT Cuts at: 1563 5541 5914 1563 Size: 3978 373 3063 Fragments arranged by size: 3978 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2121 3851 4517 5477 6861 7129 1632 Size: 489 1730 666 960 1384 268 1917 Fragments arranged by size: 1917 1730 1384 960 666 489 268 AflII C'TTAA_G Cuts at: 820 1017 3110 820 Size: 197 2093 5124 Fragments arranged by size: 5124 2093 197 AflIII A'CryG_T Cuts at: 1203 6313 7290 1203 Size: 5110 977 1327 Fragments arranged by size: 5110 1327 977 AgeI A'CCGG_T Cuts at: 6245 6245 Size: 7414 AhdI GACnn_n'nnGTC Cuts at: 6143 6143 Size: 7414 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7414 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2913 1184 Size: 1729 5685 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4544 4576 4544 Size: 32 7382 AlwNI CAG_nnn'CTG Cuts at: 2560 2623 3130 3466 3490 3628 3964 4153 5157 Size: 63 507 336 24 138 336 189 1004 Cuts at: 5157 6729 2560 Size: 1572 3245 Fragments arranged by size: 3245 1572 1004 507 336 336 189 138 63 24 ApaI G_GGCC'C Cuts at: 3094 3932 3094 Size: 838 6576 ApaLI G'TGCA_C Cuts at: 5471 6627 5471 Size: 1156 6258 ApoI r'AATT_y Cuts at: 1493 4135 4723 4949 5101 7210 1493 Size: 2642 588 226 152 2109 1697 Fragments arranged by size: 2642 2109 1697 588 226 152 AseI AT'TA_AT Cuts at: 160 1032 5968 160 Size: 872 4936 1606 Fragments arranged by size: 4936 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7414 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2160 2631 3015 3582 3764 4452 Size: 73 96 121 471 384 567 182 688 Cuts at: 4452 4678 4739 1870 Size: 226 61 4545 Fragments arranged by size: 4545 688 567 471 384 226 182 121 96 73 61 BamHI G'GATC_C Cuts at: 2362 3336 4744 5111 2362 Size: 974 1408 367 4665 Fragments arranged by size: 4665 1408 974 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4668 4735 6190 618 Size: 325 217 627 2881 67 1455 1842 Fragments arranged by size: 2881 1842 1455 627 325 217 67 BanII G_rGCy'C Cuts at: 1391 2085 2167 2811 3094 3573 3932 3972 4468 Size: 694 82 644 283 479 359 40 496 Cuts at: 4468 4733 1391 Size: 265 4072 Fragments arranged by size: 4072 694 644 496 479 359 283 265 82 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6443 BbvCI CC'TCA_GC Cuts at: 2135 3558 4476 2135 Size: 1423 918 5073 Fragments arranged by size: 5073 1423 918 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5605 5639 1903 Size: 34 3668 34 3678 Fragments arranged by size: 3678 3668 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3256 5306 6516 3256 Size: 2050 1210 4154 Fragments arranged by size: 4154 2050 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7414 BfrBI ATG'CAT Cuts at: 2570 2570 Size: 7414 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2873 6025 136 Size: 107 122 71 631 605 1201 3152 1525 Fragments arranged by size: 3152 1525 1201 631 605 122 107 71 BglII A'GATC_T Cuts at: 3910 7409 3910 Size: 3499 3915 BlpI GC'TnA_GC Cuts at: 2476 2757 3707 4116 4831 2476 Size: 281 950 409 715 5059 Fragments arranged by size: 5059 950 715 409 281 Bme1580I G_kGCm'C Cuts at: 1790 3094 3932 4167 4671 5475 6631 1790 Size: 1304 838 235 504 804 1156 2573 Fragments arranged by size: 2573 1304 1156 838 804 504 235 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7414 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2022 3694 5145 Size: 416 53 83 399 24 605 1672 1451 Cuts at: 5145 6103 442 Size: 958 1753 Fragments arranged by size: 1753 1672 1451 958 605 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3506 1056 Size: 2450 4964 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3165 4351 4612 3165 Size: 1186 261 5967 Fragments arranged by size: 5967 1186 261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 2510 2951 3365 3389 3650 3742 3897 Size: 1067 204 441 414 24 261 92 155 Cuts at: 3897 3986 4142 4175 4729 6074 1239 Size: 89 156 33 554 1345 2579 Fragments arranged by size: 2579 1345 1067 554 441 414 261 204 156 155 92 89 33 24 Bpu10I CC'TnA_GC Cuts at: 2135 3402 3558 4294 4326 4476 2135 Size: 1267 156 736 32 150 5073 Fragments arranged by size: 5073 1267 736 156 150 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3720 4115 4233 5539 6404 6702 3720 Size: 395 118 1306 865 298 4432 Fragments arranged by size: 4432 1306 865 395 298 118 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3197 6077 882 Size: 617 1698 2880 2219 Fragments arranged by size: 2880 2219 1698 617 BsaAI yAC'GTr Cuts at: 493 1204 2345 4259 5205 493 Size: 711 1141 1914 946 2702 Fragments arranged by size: 2702 1914 1141 946 711 BsaBI GATnn'nnATC Cuts at: 7408 7408 Size: 7414 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5221 5603 6238 275 Size: 53 83 186 936 3688 382 635 1451 Fragments arranged by size: 3688 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5847 6245 6519 6666 7077 5847 Size: 398 274 147 411 6184 Fragments arranged by size: 6184 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2483 2513 3796 3826 4544 Size: 30 165 30 1080 30 1283 30 718 Cuts at: 4544 4574 1178 Size: 30 4018 Fragments arranged by size: 4018 1283 1080 718 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2507 2948 3152 3647 3732 3803 3808 3895 Size: 1321 441 204 495 85 71 5 87 Cuts at: 3895 4327 4705 1186 Size: 432 378 3895 Fragments arranged by size: 3895 1321 495 441 432 378 204 87 85 71 5 BseYI C'CCAG_C Cuts at: 2112 2166 3020 3093 3207 3998 4361 4458 6617 Size: 54 854 73 114 791 363 97 2159 Cuts at: 6617 7113 2112 Size: 496 2413 Fragments arranged by size: 2413 2159 854 791 496 363 114 97 73 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3248 3332 3383 3996 1163 Size: 573 1512 84 51 613 4581 Fragments arranged by size: 4581 1512 613 573 84 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2680 4864 5625 6653 1540 Size: 275 186 679 2184 761 1028 2301 Fragments arranged by size: 2301 2184 1028 761 679 275 186 BsiHKAI G_wGCw'C Cuts at: 2811 3105 3573 5475 5560 6631 2811 Size: 294 468 1902 85 1071 3594 Fragments arranged by size: 3594 1902 1071 468 294 85 BsmI GAATG_Cn' Cuts at: 3949 4925 5018 3949 Size: 976 93 6345 Fragments arranged by size: 6345 976 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2386 2386 Size: 7414 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2167 2811 3094 3105 3573 3932 Size: 399 295 82 644 283 11 468 359 Cuts at: 3932 3972 4167 4468 4671 4733 5475 5560 6631 Size: 40 195 301 203 62 742 85 1071 Cuts at: 6631 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 644 468 399 359 301 295 283 203 195 85 82 62 40 11 BspEI T'CCGG_A Cuts at: 7077 7077 Size: 7414 BspHI T'CATG_A Cuts at: 2197 5303 7033 2197 Size: 3106 1730 2578 Fragments arranged by size: 3106 2578 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3541 4769 844 Size: 2697 1228 3489 Fragments arranged by size: 3489 2697 1228 BsrBI CCG'CTC Cuts at: 1394 1823 2761 3004 3388 5301 1394 Size: 429 938 243 384 1913 3507 Fragments arranged by size: 3507 1913 938 429 384 243 BsrDI GCAATG_nn' Cuts at: 65 1248 5909 6083 65 Size: 1183 4661 174 1396 Fragments arranged by size: 4661 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2139 2745 6058 6245 1700 Size: 110 329 606 3313 187 2869 Fragments arranged by size: 3313 2869 606 329 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7414 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7414 BssSI C'ACGA_G Cuts at: 5474 6486 5474 Size: 1012 6402 BstAPI GCAn_nnn'nTGC Cuts at: 794 3670 4105 4150 794 Size: 2876 435 45 4058 Fragments arranged by size: 4058 2876 435 45 BstBI TT'CG_AA Cuts at: 6218 6218 Size: 7414 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7414 BstXI CCAn_nnnn'nTGG Cuts at: 3823 3823 Size: 7414 BstYI r'GATC_y Cuts at: 1972 2050 2362 3336 3910 4066 4480 4575 4744 Size: 78 312 974 574 156 414 95 169 Cuts at: 4744 5111 5496 5513 6965 7051 7063 7409 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 974 574 414 385 367 346 312 169 156 95 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 3434 3771 4059 3434 Size: 337 288 6789 Fragments arranged by size: 6789 337 288 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 2661 2868 3816 3822 4299 Size: 552 939 150 507 207 948 6 477 Cuts at: 4299 513 Size: 3628 Fragments arranged by size: 3628 948 939 552 507 477 207 150 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6675 Fragments arranged by size: 6675 683 56 BtsI GCAGTG_nn' Cuts at: 4360 4372 4930 5724 5744 4360 Size: 12 558 794 20 6030 Fragments arranged by size: 6030 794 558 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7414 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7379 DraI TTT'AAA Cuts at: 4718 4718 Size: 7414 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6421 809 Size: 507 5105 1802 Fragments arranged by size: 5105 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2522 3492 3819 Size: 54 1425 50 275 18 692 970 327 Cuts at: 3819 4525 4861 5750 7379 8 Size: 706 336 889 1629 43 Fragments arranged by size: 1629 1425 970 889 706 692 336 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4861 1537 Size: 275 3049 4090 Fragments arranged by size: 4090 3049 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 2994 3060 3167 4493 5344 1353 Size: 1041 600 66 107 1326 851 3423 Fragments arranged by size: 3423 1326 1041 851 600 107 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2060 2162 6001 6375 6521 387 Size: 1064 138 471 102 3839 374 146 1280 Fragments arranged by size: 3839 1280 1064 471 374 146 138 102 EcoICRI GAG'CTC Cuts at: 2809 3571 2809 Size: 762 6652 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2306 2510 2951 3365 3389 3650 Size: 393 489 185 204 441 414 24 261 Cuts at: 3650 3742 3851 3897 3986 4142 4175 4517 4729 Size: 92 109 46 89 156 33 342 212 Cuts at: 4729 5477 6074 6861 7129 1239 Size: 748 597 787 268 1524 Fragments arranged by size: 1524 787 748 597 489 441 414 393 342 268 261 212 204 185 156 109 92 89 46 33 24 EcoNI CCTnn'n_nnAGG Cuts at: 2199 2861 3433 2199 Size: 662 572 6180 Fragments arranged by size: 6180 662 572 EcoO109I rG'GnC_Cy Cuts at: 2528 2875 3463 3929 4002 4020 4087 4126 4321 Size: 347 588 466 73 18 67 39 195 Cuts at: 4321 2528 Size: 5621 Fragments arranged by size: 5621 588 466 347 195 73 67 39 18 EcoRI G'AATT_C Cuts at: 4723 5101 4723 Size: 378 7036 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2308 2308 Size: 7414 FspI TGC'GCA Cuts at: 5920 5920 Size: 7414 HaeII r_GCGC'y Cuts at: 1678 2382 2611 3131 3884 6561 1678 Size: 704 229 520 753 2677 2531 Fragments arranged by size: 2677 2531 753 704 520 229 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3022 3054 3796 3828 6055 Size: 32 135 32 1450 32 742 32 2227 Cuts at: 6055 6087 6129 6161 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2227 1450 742 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4192 4758 5004 669 Size: 988 281 2254 566 246 3079 Fragments arranged by size: 3079 2254 988 566 281 246 HindIII A'AGCT_T Cuts at: 748 2236 748 Size: 1488 5926 HpaI GTT'AAC Cuts at: 5004 5004 Size: 7414 KpnI G_GTAC'C Cuts at: 4739 4739 Size: 7414 MfeI C'AATT_G Cuts at: 5013 5013 Size: 7414 MluI A'CGCG_T Cuts at: 7290 7290 Size: 7414 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2227 2943 3027 3135 3330 6528 6712 1305 Size: 922 716 84 108 195 3198 184 2007 Fragments arranged by size: 3198 2007 922 716 195 184 108 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 3494 10 Size: 54 1425 343 1662 3930 Fragments arranged by size: 3930 1662 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3687 3821 5372 5731 5890 518 Size: 648 2521 134 1551 359 159 2042 Fragments arranged by size: 2521 2042 1551 648 359 159 134 MspA1I CmG'CkG Cuts at: 1606 2018 2170 2980 3295 3334 3343 3367 3376 Size: 412 152 810 315 39 9 24 9 Cuts at: 3376 3457 3628 3640 3703 3998 4120 4414 4515 Size: 81 171 12 63 295 122 294 101 Cuts at: 4515 4830 5507 6655 6900 1606 Size: 315 677 1148 245 2120 Fragments arranged by size: 2120 1148 810 677 412 315 315 295 294 245 171 152 122 101 81 63 39 24 12 9 9 NaeI GCC'GGC Cuts at: 2141 2141 Size: 7414 NcoI C'CATG_G Cuts at: 513 1065 2004 2868 3816 4299 513 Size: 552 939 864 948 483 3628 Fragments arranged by size: 3628 948 939 864 552 483 NdeI CA'TA_TG Cuts at: 387 387 Size: 7414 NgoMIV G'CCGG_C Cuts at: 2139 2139 Size: 7414 NheI G'CTAG_C Cuts at: 1052 3502 1052 Size: 2450 4964 NotI GC'GGCC_GC Cuts at: 4861 4861 Size: 7414 NsiI A_TGCA'T Cuts at: 2572 2572 Size: 7414 NspI r_CATG'y Cuts at: 1132 1229 4772 6317 1132 Size: 97 3543 1545 2229 Fragments arranged by size: 3543 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2078 1348 Size: 730 6684 PciI A'CATG_T Cuts at: 6313 6313 Size: 7414 PflMI CCAn_nnn'nTGG Cuts at: 1108 2488 3628 3964 4153 1108 Size: 1380 1140 336 189 4369 Fragments arranged by size: 4369 1380 1140 336 189 PfoI T'CCnGG_A Cuts at: 4069 4069 Size: 7414 PmeI GTTT'AAAC Cuts at: 4718 4718 Size: 7414 PmlI CAC'GTG Cuts at: 2345 2345 Size: 7414 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4544 4576 5483 5515 6996 7028 4544 Size: 32 907 32 1481 32 4930 Fragments arranged by size: 4930 1481 907 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3463 4321 3463 Size: 858 6556 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7414 PsiI TTA'TAA Cuts at: 4984 4984 Size: 7414 PspOMI G'GGCC_C Cuts at: 3090 3928 3090 Size: 838 6576 PstI C_TGCA'G Cuts at: 830 2628 2910 2997 3135 3294 3462 3612 3666 Size: 1798 282 87 138 159 168 150 54 Cuts at: 3666 3678 4766 830 Size: 12 1088 3478 Fragments arranged by size: 3478 1798 1088 282 168 159 150 138 87 54 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7414 PvuII CAG'CTG Cuts at: 2018 2170 3295 3343 3367 3457 3628 3703 3998 Size: 152 1125 48 24 90 171 75 295 Cuts at: 3998 4120 4414 2018 Size: 122 294 5018 Fragments arranged by size: 5018 1125 295 294 171 152 122 90 75 48 24 SacI G_AGCT'C Cuts at: 2811 3573 2811 Size: 762 6652 SalI G'TCGA_C Cuts at: 1936 4190 4756 1936 Size: 2254 566 4594 Fragments arranged by size: 4594 2254 566 SanDI GG'GwC_CC Cuts at: 4321 4321 Size: 7414 SapI GCTCTTCn'nnn_ Cuts at: 2994 3167 2994 Size: 173 7241 SbfI CC_TGCA'GG Cuts at: 2997 4766 2997 Size: 1769 5645 ScaI AGT'ACT Cuts at: 5662 7374 5662 Size: 1712 5702 SfcI C'TryA_G Cuts at: 826 1045 2624 2645 2906 2993 3131 3290 3458 Size: 219 1579 21 261 87 138 159 168 Cuts at: 3458 3608 3662 3674 4623 4762 5897 6578 6769 Size: 150 54 12 949 139 1135 681 191 Cuts at: 6769 826 Size: 1471 Fragments arranged by size: 1579 1471 1135 949 681 261 219 191 168 159 150 139 138 87 54 21 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2873 2873 Size: 7414 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7414 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7414 SmaI CCC'GGG Cuts at: 1872 3584 3766 4741 1872 Size: 1712 182 975 4545 Fragments arranged by size: 4545 1712 975 182 SmlI C'TyrA_G Cuts at: 820 1017 1943 3110 3735 4130 4248 5518 6419 Size: 197 926 1167 625 395 118 1270 901 Cuts at: 6419 6681 820 Size: 262 1553 Fragments arranged by size: 1553 1270 1167 926 901 625 395 262 197 118 SnaBI TAC'GTA Cuts at: 493 5205 493 Size: 4712 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7414 SphI G_CATG'C Cuts at: 1132 4772 1132 Size: 3640 3774 SspI AAT'ATT Cuts at: 5 52 5338 5 Size: 47 5286 2081 Fragments arranged by size: 5286 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7414 StyI C'CwwG_G Cuts at: 513 1065 2004 2868 3816 4299 4406 4853 513 Size: 552 939 864 948 483 107 447 3074 Fragments arranged by size: 3074 948 939 864 552 483 447 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4559 5441 5458 5611 5796 6241 325 Size: 1255 2979 882 17 153 185 445 1498 Fragments arranged by size: 2979 1498 1255 882 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5660 7372 96 Size: 274 80 33 51 1443 3683 1712 138 Fragments arranged by size: 3683 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4257 4289 4648 4680 7374 7406 4257 Size: 32 359 32 2694 32 4265 Fragments arranged by size: 4265 2694 359 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7414 XbaI T'CTAG_A Cuts at: 4750 4750 Size: 7414 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7414 XmaI C'CCGG_G Cuts at: 1870 3582 3764 4739 1870 Size: 1712 182 975 4545 Fragments arranged by size: 4545 1712 975 182 XmnI GAAnn'nnTTC Cuts at: 2307 5543 2307 Size: 3236 4178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5222 276 Size: 53 83 186 936 3688 2468 Fragments arranged by size: 3688 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI XcmI