(Circular) (Six-base) MAPSORT of: pFN21AB5941.seq Check: 1915 from: 1 to: 6229 pFN21AB5941 6229 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2995 2995 Size: 6229 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2531 2556 4039 278 Size: 53 83 186 936 995 25 1483 2468 Fragments arranged by size: 2468 1483 995 936 186 83 53 25 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2858 4292 5676 5944 1632 Size: 1226 1434 1384 268 1917 Fragments arranged by size: 1917 1434 1384 1226 268 AfeI AGC'GCT Cuts at: 2212 3014 2212 Size: 802 5427 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 2343 5128 6105 1203 Size: 1140 2785 977 1327 Fragments arranged by size: 2785 1327 1140 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3235 1184 Size: 2051 4178 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 2090 Size: 32 6197 AlwNI CAG_nnn'CTG Cuts at: 3167 3494 3972 5544 3167 Size: 327 478 1572 3852 Fragments arranged by size: 3852 1572 478 327 ApaI G_GGCC'C Cuts at: 2145 2225 3065 2145 Size: 80 840 5309 Fragments arranged by size: 5309 840 80 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 3538 3764 3916 6025 1493 Size: 2045 226 152 2109 1697 Fragments arranged by size: 2109 2045 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2136 2224 2252 2863 3554 1870 Size: 73 72 121 88 28 611 691 4545 Fragments arranged by size: 4545 691 611 121 88 73 72 28 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 3550 5005 618 Size: 325 217 627 290 1473 1455 1842 Fragments arranged by size: 1842 1473 1455 627 325 290 217 BanII G_rGCy'C Cuts at: 1391 2145 2225 2836 2870 2960 3065 3433 3548 Size: 754 80 611 34 90 105 368 115 Cuts at: 3548 1391 Size: 4072 Fragments arranged by size: 4072 754 611 368 115 105 90 80 34 BbeI G_GCGC'C Cuts at: 2081 2081 Size: 6229 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5258 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2338 2372 4420 4454 1903 Size: 34 401 34 2048 34 3678 Fragments arranged by size: 3678 2048 401 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3094 4121 5331 3094 Size: 1027 1210 3992 Fragments arranged by size: 3992 1210 1027 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 2129 4840 Size: 107 122 71 631 605 403 54 2711 Cuts at: 4840 136 Size: 1525 Fragments arranged by size: 2711 1525 631 605 403 122 107 71 54 BglII A'GATC_T Cuts at: 2847 6224 2847 Size: 3377 2852 BlpI GC'TnA_GC Cuts at: 2624 2679 3495 3646 2624 Size: 55 816 151 5207 Fragments arranged by size: 5207 816 151 55 Bme1580I G_kGCm'C Cuts at: 1790 2145 2225 2361 3065 3332 4290 5446 1790 Size: 355 80 136 704 267 958 1156 2573 Fragments arranged by size: 2573 1156 958 704 355 267 136 80 BmgBI CAC'GTC Cuts at: 1318 2346 1318 Size: 1028 5201 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3141 3960 4918 Size: 416 53 83 399 24 1724 819 958 Cuts at: 4918 442 Size: 1753 Fragments arranged by size: 1753 1724 958 819 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3277 3277 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2621 2722 3092 4889 1239 Size: 1382 101 370 1797 2579 Fragments arranged by size: 2579 1797 1382 370 101 Bpu10I CC'TnA_GC Cuts at: 3333 3412 3333 Size: 79 6150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4354 5219 5517 4354 Size: 865 298 5066 Fragments arranged by size: 5066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4892 882 Size: 617 3393 2219 Fragments arranged by size: 3393 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2488 4020 493 Size: 711 1284 1532 2702 Fragments arranged by size: 2702 1532 1284 711 BsaBI GATnn'nnATC Cuts at: 3105 6223 3105 Size: 3118 3111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 2078 2442 2528 Size: 53 83 186 936 488 57 364 86 Cuts at: 2528 2553 2735 4036 4418 5053 275 Size: 25 182 1301 382 635 1451 Fragments arranged by size: 1451 1301 936 635 488 382 364 186 182 86 83 57 53 25 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 2708 2738 3284 Size: 30 165 30 822 30 453 30 546 Cuts at: 3284 3314 1178 Size: 30 4093 Fragments arranged by size: 4093 822 546 453 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6229 BseYI C'CCAG_C Cuts at: 2063 2213 3018 3245 3296 3303 3487 3509 5432 Size: 150 805 227 51 7 184 22 1923 Cuts at: 5432 5928 2063 Size: 496 2364 Fragments arranged by size: 2364 1923 805 496 227 184 150 51 22 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2267 2947 1163 Size: 573 531 680 4445 Fragments arranged by size: 4445 680 573 531 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2562 3679 4440 5468 1540 Size: 275 186 561 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 561 275 186 BsiHKAI G_wGCw'C Cuts at: 2436 2811 4290 4375 5446 2436 Size: 375 1479 85 1071 3219 Fragments arranged by size: 3219 1479 1071 375 85 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 BsmBI CGTCTCn'nnnn_ Cuts at: 2535 2560 2535 Size: 25 6204 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2225 2361 2436 2811 2836 2870 Size: 399 355 80 136 75 375 25 34 Cuts at: 2870 2960 3065 3332 3433 3548 4290 4375 5446 Size: 90 105 267 101 115 742 85 1071 Cuts at: 5446 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 375 355 267 136 115 105 101 90 85 80 75 34 25 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2640 2995 3584 844 Size: 1796 355 589 3489 Fragments arranged by size: 3489 1796 589 355 BsrBI CCG'CTC Cuts at: 1394 1823 2170 2481 4116 1394 Size: 429 347 311 1635 3507 Fragments arranged by size: 3507 1635 429 347 311 BsrDI GCAATG_nn' Cuts at: 65 1248 4724 4898 65 Size: 1183 3476 174 1396 Fragments arranged by size: 3476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2561 4873 5060 1700 Size: 110 751 2312 187 2869 Fragments arranged by size: 2869 2312 751 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6229 BssHII G'CGCG_C Cuts at: 1925 2667 1925 Size: 742 5487 BssSI C'ACGA_G Cuts at: 2301 2780 4289 5301 2301 Size: 479 1509 1012 3229 Fragments arranged by size: 3229 1509 1012 479 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstXI CCAn_nnnn'nTGG Cuts at: 2939 3517 2939 Size: 578 5651 BstYI r'GATC_y Cuts at: 1972 2595 2847 3559 3926 4311 4328 5780 5866 Size: 623 252 712 367 385 17 1452 86 Cuts at: 5866 5878 6224 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 712 623 385 367 346 252 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2264 2295 2448 3192 3317 513 Size: 552 939 260 31 153 744 125 3425 Fragments arranged by size: 3425 939 744 552 260 153 125 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 3042 3091 3181 3745 4539 4559 3042 Size: 49 90 564 794 20 4712 Fragments arranged by size: 4712 794 564 90 49 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DraIII CAC_nnn'GTG Cuts at: 2491 2616 2491 Size: 125 6104 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2482 2559 3189 Size: 54 1425 50 275 18 652 77 630 Cuts at: 3189 3676 4565 6194 8 Size: 487 889 1629 43 Fragments arranged by size: 1629 1425 889 652 630 487 275 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2559 3676 1537 Size: 275 747 1117 4090 Fragments arranged by size: 4090 1117 747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2142 2897 4159 1353 Size: 789 755 1262 3423 Fragments arranged by size: 3423 1262 789 755 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2621 2722 2858 3092 4292 4889 5676 Size: 393 989 101 136 234 1200 597 787 Cuts at: 5676 5944 1239 Size: 268 1524 Fragments arranged by size: 1524 1200 989 787 597 393 268 234 136 101 EcoNI CCTnn'n_nnAGG Cuts at: 2285 2285 Size: 6229 EcoO109I rG'GnC_Cy Cuts at: 2141 2980 3061 3062 3154 3164 2141 Size: 839 81 1 92 10 5206 Fragments arranged by size: 5206 839 92 81 10 1 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 EcoRV GAT'ATC Cuts at: 2402 2402 Size: 6229 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2929 2929 Size: 6229 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 2081 2214 2394 2709 3016 5376 1678 Size: 403 133 180 315 307 2360 2531 Fragments arranged by size: 2531 2360 403 315 307 180 133 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2708 2740 3188 3220 3284 Size: 32 135 32 1136 32 448 32 64 Cuts at: 3284 3316 3395 3427 4870 4902 4944 4976 1373 Size: 32 79 32 1443 32 42 32 2626 Fragments arranged by size: 2626 1443 1136 448 135 79 64 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3573 3819 669 Size: 988 281 1635 246 3079 Fragments arranged by size: 3079 1635 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KasI G'GCGC_C Cuts at: 2077 2077 Size: 6229 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2154 2694 3486 5343 5527 1305 Size: 849 540 792 1857 184 2007 Fragments arranged by size: 2007 1857 849 792 540 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3191 10 Size: 54 1425 343 1359 3048 Fragments arranged by size: 3048 1425 1359 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2421 2780 2792 4187 4546 4705 518 Size: 648 1255 359 12 1395 359 159 2042 Fragments arranged by size: 2042 1395 1255 648 359 359 159 12 MspA1I CmG'CkG Cuts at: 1606 2920 3494 3645 4322 5470 5715 1606 Size: 1314 574 151 677 1148 245 2120 Fragments arranged by size: 2120 1314 1148 677 574 245 151 NaeI GCC'GGC Cuts at: 2563 2563 Size: 6229 NarI GG'CG_CC Cuts at: 2078 2078 Size: 6229 NcoI C'CATG_G Cuts at: 513 1065 2004 2264 3192 3317 513 Size: 552 939 260 928 125 3425 Fragments arranged by size: 3425 939 928 552 260 125 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NgoMIV G'CCGG_C Cuts at: 2561 2561 Size: 6229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3095 3587 5132 1132 Size: 97 1866 492 1545 2229 Fragments arranged by size: 2229 1866 1545 492 97 PasI CC'CwG_GG Cuts at: 1348 3161 3424 1348 Size: 1813 263 4153 Fragments arranged by size: 4153 1813 263 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 3167 1108 Size: 2059 4170 PfoI T'CCnGG_A Cuts at: 2094 2598 3288 2094 Size: 504 690 5035 Fragments arranged by size: 5035 690 504 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2980 3154 2980 Size: 174 6055 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PspOMI G'GGCC_C Cuts at: 2141 2221 3061 2141 Size: 80 840 5309 Fragments arranged by size: 5309 840 80 PspXI vC'TCGA_Gb Cuts at: 2252 2252 Size: 6229 PstI C_TGCA'G Cuts at: 830 3581 830 Size: 2751 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2920 2920 Size: 6229 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SapI GCTCTTCn'nnn_ Cuts at: 2142 2142 Size: 6229 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SfcI C'TryA_G Cuts at: 826 1045 2233 2334 3406 3577 4712 5393 5584 Size: 219 1188 101 1072 171 1135 681 191 Cuts at: 5584 826 Size: 1471 Fragments arranged by size: 1471 1188 1135 1072 681 219 191 171 101 SfoI GGC'GCC Cuts at: 2079 2079 Size: 6229 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 2138 2226 3556 1872 Size: 266 88 1330 4545 Fragments arranged by size: 4545 1330 266 88 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 4333 5234 5496 820 Size: 197 926 309 2081 901 262 1553 Fragments arranged by size: 2081 1553 926 901 309 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3095 3587 1132 Size: 1963 492 3774 Fragments arranged by size: 3774 1963 492 SrfI GCCC'GGGC Cuts at: 2226 2226 Size: 6229 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 2889 1517 Size: 1372 4857 StyI C'CwwG_G Cuts at: 513 1065 2004 2264 3192 3317 3433 3668 513 Size: 552 939 260 928 125 116 235 3074 Fragments arranged by size: 3074 939 928 552 260 235 125 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2536 2915 4256 4273 4426 4611 5056 Size: 1255 956 379 1341 17 153 185 445 Cuts at: 5056 325 Size: 1498 Fragments arranged by size: 1498 1341 1255 956 445 379 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3279 4475 6187 Size: 274 80 33 51 1443 1302 1196 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 1712 1443 1302 1196 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2171 2203 6189 6221 2171 Size: 32 3986 32 2179 Fragments arranged by size: 3986 2179 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6229 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 2252 1943 Size: 309 5920 XmaI C'CCGG_G Cuts at: 1870 2136 2224 3554 1870 Size: 266 88 1330 4545 Fragments arranged by size: 4545 1330 266 88 XmnI GAAnn'nnTTC Cuts at: 2151 4358 2151 Size: 2207 4022 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2529 2554 4037 276 Size: 53 83 186 936 995 25 1483 2468 Fragments arranged by size: 2468 1483 995 936 186 83 53 25 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SexAI SfiI SwaI XcmI