Restriction map for
pFN21AB5929
(Linear) (Six-base) MAPSORT of: KIBB5929.seq Check: 1683 from: 1 to: 1521
KIBB5929.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
Acc65I G'GTAC_C
Cuts at: 0 37 1521
Size: 37 1484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1030 1085 1367 1521
Size: 294 736 55 282 154
Fragments arranged by size:
736 294 282 154 55
AlwNI CAG_nnn'CTG
Cuts at: 0 109 1521
Size: 109 1412
ApoI r'AATT_y
Cuts at: 0 487 1181 1521
Size: 487 694 340
Fragments arranged by size:
694 487 340
AvaI C'yCGr_G
Cuts at: 0 24 1521
Size: 24 1497
BanI G'GyrC_C
Cuts at: 0 28 37 1521
Size: 28 9 1484
Fragments arranged by size:
1484 28 9
BanII G_rGCy'C
Cuts at: 0 21 1521
Size: 21 1500
BbsI GAAGACnn'nnnn_
Cuts at: 0 1011 1521
Size: 1011 510
BciVI GTATCCnnnnn_n'
Cuts at: 0 1236 1521
Size: 1236 285
BclI T'GATC_A
Cuts at: 0 705 1521
Size: 705 816
BglI GCCn_nnn'nGGC
Cuts at: 0 55 1210 1521
Size: 55 1155 311
Fragments arranged by size:
1155 311 55
BglII A'GATC_T
Cuts at: 0 1342 1371 1521
Size: 1342 29 150
Fragments arranged by size:
1342 150 29
Bme1580I G_kGCm'C
Cuts at: 0 31 688 1521
Size: 31 657 833
Fragments arranged by size:
833 657 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 262 330 539 1521
Size: 262 68 209 982
Fragments arranged by size:
982 262 209 68
Bpu10I CC'TnA_GC
Cuts at: 0 1420 1521
Size: 1420 101
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 1521
Size: 310 1211
BsaBI GATnn'nnATC
Cuts at: 0 1370 1435 1521
Size: 1370 65 86
Fragments arranged by size:
1370 86 65
BsaWI w'CCGG_w
Cuts at: 0 34 1521
Size: 34 1487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 312 342 1521
Size: 312 30 1179
Fragments arranged by size:
1179 312 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 1521
Size: 854 667
BsiHKAI G_wGCw'C
Cuts at: 0 369 1521
Size: 369 1152
BsmI GAATG_Cn'
Cuts at: 0 112 1521
Size: 112 1409
Bsp1286I G_dGCh'C
Cuts at: 0 21 31 369 688 1521
Size: 21 10 338 319 833
Fragments arranged by size:
833 338 319 21 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 1521
Size: 94 1427
BsrDI GCAATG_nn'
Cuts at: 0 634 694 1521
Size: 634 60 827
Fragments arranged by size:
827 634 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 109 1521
Size: 109 1412
BstBI TT'CG_AA
Cuts at: 0 606 1521
Size: 606 915
BstYI r'GATC_y
Cuts at: 0 1140 1342 1371 1521
Size: 1140 202 29 150
Fragments arranged by size:
1140 202 150 29
BtgI C'CryG_G
Cuts at: 0 53 62 1521
Size: 53 9 1459
Fragments arranged by size:
1459 53 9
EarI CTCTTCn'nnn_
Cuts at: 0 313 756 1186 1214 1521
Size: 313 443 430 28 307
Fragments arranged by size:
443 430 313 307 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 467 1521
Size: 467 1054
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1030 1085 1367 1521
Size: 294 736 55 282 154
Fragments arranged by size:
736 294 282 154 55
EcoO109I rG'GnC_Cy
Cuts at: 0 1164 1521
Size: 1164 357
EcoRI G'AATT_C
Cuts at: 0 487 1521
Size: 487 1034
EcoRV GAT'ATC
Cuts at: 0 1056 1521
Size: 1056 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1040 1045 1072 1077 1308 1340 1496 1521
Size: 1040 5 27 5 231 32 156 25
Fragments arranged by size:
1040 231 156 32 27 25 5 5
KpnI G_GTAC'C
Cuts at: 0 41 1521
Size: 41 1480
MfeI C'AATT_G
Cuts at: 0 817 1521
Size: 817 704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 403 1521
Size: 365 38 1118
Fragments arranged by size:
1118 365 38
MspA1I CmG'CkG
Cuts at: 0 43 55 64 348 1239 1521
Size: 43 12 9 284 891 282
Fragments arranged by size:
891 284 282 43 12 9
NspI r_CATG'y
Cuts at: 0 543 1521
Size: 543 978
PpuMI rG'GwC_Cy
Cuts at: 0 1164 1521
Size: 1164 357
PstI C_TGCA'G
Cuts at: 0 48 105 434 1521
Size: 48 57 329 1087
Fragments arranged by size:
1087 329 57 48
PvuII CAG'CTG
Cuts at: 0 348 1239 1521
Size: 348 891 282
Fragments arranged by size:
891 348 282
SacII CC_GC'GG
Cuts at: 0 56 65 1521
Size: 56 9 1456
Fragments arranged by size:
1456 56 9
SapI GCTCTTCn'nnn_
Cuts at: 0 1214 1521
Size: 1214 307
ScaI AGT'ACT
Cuts at: 0 222 1521
Size: 222 1299
SfcI C'TryA_G
Cuts at: 0 44 101 430 1103 1521
Size: 44 57 329 673 418
Fragments arranged by size:
673 418 329 57 44
SpeI A'CTAG_T
Cuts at: 0 217 1521
Size: 217 1304
SphI G_CATG'C
Cuts at: 0 543 1521
Size: 543 978
SspI AAT'ATT
Cuts at: 0 474 1521
Size: 474 1047
StuI AGG'CCT
Cuts at: 0 436 1418 1521
Size: 436 982 103
Fragments arranged by size:
982 436 103
StyI C'CwwG_G
Cuts at: 0 935 1521
Size: 935 586
TatI w'GTAC_w
Cuts at: 0 220 1521
Size: 220 1301
XbaI T'CTAG_A
Cuts at: 0 1452 1521
Size: 1452 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1504 1521
Size: 1504 17
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI AvaI BanI BanII BbsI
BciVI BclI BglI BglII Bme1580I BmrI Bpu10I BsaI
BsaBI BsaWI BsaXI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BstAPI BstBI BstYI BtgI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV Hin4I KpnI MfeI MmeI MspA1I
NspI PpuMI PstI PvuII SacII SapI ScaI SfcI
SpeI SphI SspI StuI StyI TatI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI
BlpI BmgBI BmtI BplI BpmI BpuEI BsaAI BsaHI
BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EcoICRI EcoNI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SrfI SwaI TaqII TstI Tth111I
XhoI XmaI XmnI ZraI