(Circular) (Six-base) MAPSORT of: pFN21AB5799.seq Check: 5902 from: 1 to: 7207 pFN21AB5799 7207 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2874 3597 5017 278 Size: 53 83 186 936 1338 723 1420 2468 Fragments arranged by size: 2468 1420 1338 936 723 186 83 53 AccI GT'mk_AC Cuts at: 1937 3888 4550 1937 Size: 1951 662 4594 Fragments arranged by size: 4594 1951 662 Acc65I G'GTAC_C Cuts at: 4528 4528 Size: 7207 AclI AA'CG_TT Cuts at: 1563 4461 5334 5707 1563 Size: 2898 873 373 3063 Fragments arranged by size: 3063 2898 873 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 2768 5270 6654 6922 1632 Size: 953 183 2502 1384 268 1917 Fragments arranged by size: 2502 1917 1384 953 268 183 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7010 AflIII A'CryG_T Cuts at: 1203 2828 6106 7083 1203 Size: 1625 3278 977 1327 Fragments arranged by size: 3278 1625 1327 977 AgeI A'CCGG_T Cuts at: 6038 6038 Size: 7207 AhdI GACnn_n'nnGTC Cuts at: 5936 5936 Size: 7207 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7207 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3872 1184 Size: 2688 4519 AlwNI CAG_nnn'CTG Cuts at: 2902 2920 3000 3541 4003 4950 6522 2902 Size: 18 80 541 462 947 1572 3587 Fragments arranged by size: 3587 1572 947 541 462 80 18 ApaI G_GGCC'C Cuts at: 2102 4170 2102 Size: 2068 5139 ApaLI G'TGCA_C Cuts at: 5264 6420 5264 Size: 1156 6051 ApoI r'AATT_y Cuts at: 1493 2173 2642 3906 4019 4516 4742 4894 7003 Size: 680 469 1264 113 497 226 152 2109 Cuts at: 7003 1493 Size: 1697 Fragments arranged by size: 2109 1697 1264 680 497 469 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 3393 5761 160 Size: 872 2361 2368 1606 Fragments arranged by size: 2368 2361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7207 AvaI C'yCGr_G Cuts at: 1870 1943 3564 3621 4532 1870 Size: 73 1621 57 911 4545 Fragments arranged by size: 4545 1621 911 73 57 BamHI G'GATC_C Cuts at: 4537 4904 4537 Size: 367 6840 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4528 5983 618 Size: 325 217 627 2741 1455 1842 Fragments arranged by size: 2741 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2102 2760 2856 4053 4170 4526 1391 Size: 711 658 96 1197 117 356 4072 Fragments arranged by size: 4072 1197 711 658 356 117 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2610 3235 4213 928 Size: 971 711 625 978 3922 Fragments arranged by size: 3922 978 971 711 625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4229 4263 5398 5432 1903 Size: 34 2292 34 1135 34 3678 Fragments arranged by size: 3678 2292 1135 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2174 5099 6309 2174 Size: 2925 1210 3072 Fragments arranged by size: 3072 2925 1210 BclI T'GATC_A Cuts at: 1548 3825 1548 Size: 2277 4930 BfrBI ATG'CAT Cuts at: 3467 3467 Size: 7207 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4165 5818 136 Size: 107 122 71 631 605 2493 1653 1525 Fragments arranged by size: 2493 1653 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7202 7202 Size: 7207 BlpI GC'TnA_GC Cuts at: 2759 4624 2759 Size: 1865 5342 Bme1580I G_kGCm'C Cuts at: 1790 2102 3034 3448 3715 3729 4170 5268 6424 Size: 312 932 414 267 14 441 1098 1156 Cuts at: 6424 1790 Size: 2573 Fragments arranged by size: 2573 1156 1098 932 441 414 312 267 14 BmgBI CAC'GTC Cuts at: 1318 2831 1318 Size: 1513 5694 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2084 2290 3240 Size: 416 53 83 399 24 667 206 950 Cuts at: 3240 4938 5896 442 Size: 1698 958 1753 Fragments arranged by size: 1753 1698 958 950 667 416 399 206 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7207 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2730 2730 Size: 7207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2444 3299 5867 1239 Size: 1013 192 855 2568 2579 Fragments arranged by size: 2579 2568 1013 855 192 Bpu10I CC'TnA_GC Cuts at: 2813 2813 Size: 7207 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2243 3622 5332 6197 6495 2243 Size: 1379 1710 865 298 2955 Fragments arranged by size: 2955 1710 1379 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2044 4187 4330 5870 882 Size: 617 545 2143 143 1540 2219 Fragments arranged by size: 2219 2143 1540 617 545 143 BsaAI yAC'GTr Cuts at: 493 1204 4091 4998 493 Size: 711 2887 907 2702 Fragments arranged by size: 2887 2702 907 711 BsaBI GATnn'nnATC Cuts at: 7201 7201 Size: 7207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2475 2871 3594 3891 Size: 53 83 186 936 942 396 723 297 Cuts at: 3891 5014 5396 6031 275 Size: 1123 382 635 1451 Fragments arranged by size: 1451 1123 942 936 723 635 396 382 297 186 83 53 BsaWI w'CCGG_w Cuts at: 5640 6038 6312 6459 6870 5640 Size: 398 274 147 411 5977 Fragments arranged by size: 5977 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2427 2457 3566 3596 1178 Size: 30 165 30 1024 30 1109 30 4789 Fragments arranged by size: 4789 1109 1024 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2056 2129 2132 2144 2147 2150 2153 2525 Size: 870 73 3 12 3 3 3 372 Cuts at: 2525 3437 3881 1186 Size: 912 444 4512 Fragments arranged by size: 4512 912 870 444 372 73 12 3 3 3 3 BseYI C'CCAG_C Cuts at: 2078 3472 4286 6410 6906 2078 Size: 1394 814 2124 496 2379 Fragments arranged by size: 2379 2124 1394 814 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3101 3416 3467 3978 4196 1163 Size: 573 1365 315 51 511 218 4174 Fragments arranged by size: 4174 1365 573 511 315 218 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4657 5418 6446 1540 Size: 275 186 2656 761 1028 2301 Fragments arranged by size: 2656 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 2551 2856 4074 5268 5353 6424 2370 Size: 181 305 1218 1194 85 1071 3153 Fragments arranged by size: 3153 1218 1194 1071 305 181 85 BsmI GAATG_Cn' Cuts at: 4718 4811 4718 Size: 93 7114 BsmBI CGTCTCn'nnnn_ Cuts at: 3500 3500 Size: 7207 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2370 2551 2760 2856 3034 3448 Size: 399 312 268 181 209 96 178 414 Cuts at: 3448 3715 3729 4053 4074 4170 4526 5268 5353 Size: 267 14 324 21 96 356 742 85 Cuts at: 5353 6424 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 414 399 356 324 312 268 267 209 181 178 96 96 85 21 14 BspEI T'CCGG_A Cuts at: 6870 6870 Size: 7207 BspHI T'CATG_A Cuts at: 2839 3948 4467 5096 6826 2839 Size: 1109 519 629 1730 3220 Fragments arranged by size: 3220 1730 1109 629 519 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2995 3123 3489 4562 844 Size: 2151 128 366 1073 3489 Fragments arranged by size: 3489 2151 1073 366 128 BsrBI CCG'CTC Cuts at: 1394 1823 3057 5094 1394 Size: 429 1234 2037 3507 Fragments arranged by size: 3507 2037 1234 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2190 3090 3467 5702 5876 65 Size: 1183 942 900 377 2235 174 1396 Fragments arranged by size: 2235 1396 1183 942 900 377 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5851 6038 1700 Size: 110 4041 187 2869 Fragments arranged by size: 4041 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2727 3024 96 Size: 2631 297 4279 Fragments arranged by size: 4279 2631 297 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7207 BssSI C'ACGA_G Cuts at: 2249 3546 4311 5267 6279 2249 Size: 1297 765 956 1012 3177 Fragments arranged by size: 3177 1297 1012 956 765 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7207 BstBI TT'CG_AA Cuts at: 6011 6011 Size: 7207 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7207 BstXI CCAn_nnnn'nTGG Cuts at: 3460 4042 3460 Size: 582 6625 BstYI r'GATC_y Cuts at: 1972 2151 3202 3492 4441 4537 4904 5289 5306 Size: 179 1051 290 949 96 367 385 17 Cuts at: 5306 6758 6844 6856 7202 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1051 949 385 367 346 290 179 96 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2014 3041 2014 Size: 1027 6180 BtgI C'CryG_G Cuts at: 513 1065 2004 3366 4188 513 Size: 552 939 1362 822 3532 Fragments arranged by size: 3532 1362 939 822 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6468 Fragments arranged by size: 6468 683 56 BtsI GCAGTG_nn' Cuts at: 3316 3484 3984 3990 4348 4723 5517 5537 3316 Size: 168 500 6 358 375 794 20 4986 Fragments arranged by size: 4986 794 500 375 358 168 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7207 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7172 DraI TTT'AAA Cuts at: 4511 4511 Size: 7207 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2612 6214 809 Size: 507 1296 3602 1802 Fragments arranged by size: 3602 1802 1296 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2736 4062 Size: 54 1425 50 275 18 315 591 1326 Cuts at: 4062 4119 4654 5543 7172 8 Size: 57 535 889 1629 43 Fragments arranged by size: 1629 1425 1326 889 591 535 315 275 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4654 1537 Size: 275 2842 4090 Fragments arranged by size: 4090 2842 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2507 2515 3791 5137 1353 Size: 1154 8 1276 1346 3423 Fragments arranged by size: 3423 1346 1276 1154 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5794 6168 6314 387 Size: 1064 138 4205 374 146 1280 Fragments arranged by size: 4205 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2854 2854 Size: 7207 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2444 2585 2768 3299 5270 5867 Size: 393 620 192 141 183 531 1971 597 Cuts at: 5867 6654 6922 1239 Size: 787 268 1524 Fragments arranged by size: 1971 1524 787 620 597 531 393 268 192 183 141 EcoNI CCTnn'n_nnAGG Cuts at: 3257 3812 3962 3257 Size: 555 150 6502 Fragments arranged by size: 6502 555 150 EcoO109I rG'GnC_Cy Cuts at: 2099 3478 4166 4167 2099 Size: 1379 688 1 5139 Fragments arranged by size: 5139 1379 688 1 EcoRI G'AATT_C Cuts at: 2173 3906 4516 4894 2173 Size: 1733 610 378 4486 Fragments arranged by size: 4486 1733 610 378 FspI TGC'GCA Cuts at: 5713 5713 Size: 7207 HaeII r_GCGC'y Cuts at: 1678 6354 1678 Size: 4676 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2376 2408 2908 2940 3540 Size: 32 135 32 804 32 500 32 600 Cuts at: 3540 3566 3572 3598 5848 5880 5922 5954 1373 Size: 26 6 26 2250 32 42 32 2626 Fragments arranged by size: 2626 2250 804 600 500 135 42 32 32 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 2652 4551 4797 669 Size: 988 281 714 1899 246 3079 Fragments arranged by size: 3079 1899 988 714 281 246 HindIII A'AGCT_T Cuts at: 748 2261 2351 4301 748 Size: 1513 90 1950 3654 Fragments arranged by size: 3654 1950 1513 90 HpaI GTT'AAC Cuts at: 4797 4797 Size: 7207 KpnI G_GTAC'C Cuts at: 4532 4532 Size: 7207 MfeI C'AATT_G Cuts at: 4806 4806 Size: 7207 MluI A'CGCG_T Cuts at: 7083 7083 Size: 7207 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2858 4099 6321 6505 1305 Size: 1553 1241 2222 184 2007 Fragments arranged by size: 2222 2007 1553 1241 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2738 4121 10 Size: 54 1425 343 906 1383 3096 Fragments arranged by size: 3096 1425 1383 906 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2160 3833 3947 4115 5165 5524 5683 Size: 648 994 1673 114 168 1050 359 159 Cuts at: 5683 518 Size: 2042 Fragments arranged by size: 2042 1673 1050 994 648 359 168 159 114 MspA1I CmG'CkG Cuts at: 1606 3554 4067 4277 4623 5300 6448 6693 1606 Size: 1948 513 210 346 677 1148 245 2120 Fragments arranged by size: 2120 1948 1148 677 513 346 245 210 NcoI C'CATG_G Cuts at: 513 1065 2004 3366 4188 513 Size: 552 939 1362 822 3532 Fragments arranged by size: 3532 1362 939 822 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7207 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7207 NotI GC'GGCC_GC Cuts at: 4654 4654 Size: 7207 NsiI A_TGCA'T Cuts at: 3469 3469 Size: 7207 NspI r_CATG'y Cuts at: 1132 1229 4104 4565 6110 1132 Size: 97 2875 461 1545 2229 Fragments arranged by size: 2875 2229 1545 461 97 PasI CC'CwG_GG Cuts at: 1348 2103 3034 1348 Size: 755 931 5521 Fragments arranged by size: 5521 931 755 PciI A'CATG_T Cuts at: 6106 6106 Size: 7207 PflMI CCAn_nnn'nTGG Cuts at: 1108 2902 3541 1108 Size: 1794 639 4774 Fragments arranged by size: 4774 1794 639 PfoI T'CCnGG_A Cuts at: 3495 3495 Size: 7207 PmeI GTTT'AAAC Cuts at: 4511 4511 Size: 7207 PmlI CAC'GTG Cuts at: 4091 4091 Size: 7207 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5276 5308 6789 6821 5276 Size: 32 1481 32 5662 Fragments arranged by size: 5662 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3478 3478 Size: 7207 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7207 PsiI TTA'TAA Cuts at: 4777 4777 Size: 7207 PspOMI G'GGCC_C Cuts at: 2098 4166 2098 Size: 2068 5139 PstI C_TGCA'G Cuts at: 830 4559 830 Size: 3729 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7207 PvuII CAG'CTG Cuts at: 3554 4277 3554 Size: 723 6484 SacI G_AGCT'C Cuts at: 2856 2856 Size: 7207 SalI G'TCGA_C Cuts at: 1936 4549 1936 Size: 2613 4594 SapI GCTCTTCn'nnn_ Cuts at: 3791 3791 Size: 7207 SbfI CC_TGCA'GG Cuts at: 4559 4559 Size: 7207 ScaI AGT'ACT Cuts at: 5455 7167 5455 Size: 1712 5495 SexAI A'CCwGG_T Cuts at: 3963 3963 Size: 7207 SfcI C'TryA_G Cuts at: 826 1045 2693 3527 4555 5690 6371 6562 826 Size: 219 1648 834 1028 1135 681 191 1471 Fragments arranged by size: 1648 1471 1135 1028 834 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7207 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7207 SmaI CCC'GGG Cuts at: 1872 3623 4534 1872 Size: 1751 911 4545 Fragments arranged by size: 4545 1751 911 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 3637 5311 6212 6474 820 Size: 197 926 315 1379 1674 901 262 1553 Fragments arranged by size: 1674 1553 1379 926 901 315 262 197 SnaBI TAC'GTA Cuts at: 493 4998 493 Size: 4505 2702 SpeI A'CTAG_T Cuts at: 152 2207 152 Size: 2055 5152 SphI G_CATG'C Cuts at: 1132 4565 1132 Size: 3433 3774 SspI AAT'ATT Cuts at: 5 52 5131 5 Size: 47 5079 2081 Fragments arranged by size: 5079 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7207 StyI C'CwwG_G Cuts at: 513 1065 2004 2027 2988 3366 3453 3664 3931 Size: 552 939 23 961 378 87 211 267 Cuts at: 3931 4188 4646 513 Size: 257 458 3074 Fragments arranged by size: 3074 961 939 552 458 378 267 257 211 87 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2062 5234 5251 5404 5589 6034 325 Size: 1255 482 3172 17 153 185 445 1498 Fragments arranged by size: 3172 1498 1255 482 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2727 3024 5453 Size: 274 80 33 51 1443 750 297 2429 Cuts at: 5453 7165 96 Size: 1712 138 Fragments arranged by size: 2429 1712 1443 750 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7167 7199 7167 Size: 32 7175 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7207 XbaI T'CTAG_A Cuts at: 4543 4543 Size: 7207 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7207 XmaI C'CCGG_G Cuts at: 1870 3621 4532 1870 Size: 1751 911 4545 Fragments arranged by size: 4545 1751 911 XmnI GAAnn'nnTTC Cuts at: 4463 5336 4463 Size: 873 6334 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2872 3595 5015 276 Size: 53 83 186 936 1338 723 1420 2468 Fragments arranged by size: 2468 1420 1338 936 723 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI