(Circular) (Six-base) MAPSORT of: pFN21AB5922.seq Check: 820 from: 1 to: 6304 pFN21AB5922 6304 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4114 278 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3647 1937 Size: 1710 4594 Acc65I G'GTAC_C Cuts at: 3597 3625 3597 Size: 28 6276 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2301 3502 4367 5751 6019 1632 Size: 669 1201 865 1384 268 1917 Fragments arranged by size: 1917 1384 1201 865 669 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 5203 6180 1203 Size: 4000 977 1327 Fragments arranged by size: 4000 1327 977 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 2389 3490 5033 2389 Size: 1101 1543 3660 Fragments arranged by size: 3660 1543 1101 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2918 1184 Size: 1734 4570 AlwNI CAG_nnn'CTG Cuts at: 3305 4047 5619 3305 Size: 742 1572 3990 Fragments arranged by size: 3990 1572 742 ApaLI G'TGCA_C Cuts at: 3574 4361 5517 3574 Size: 787 1156 4361 Fragments arranged by size: 4361 1156 787 ApoI r'AATT_y Cuts at: 1493 3250 3613 3839 3991 6100 1493 Size: 1757 363 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 363 226 152 AseI AT'TA_AT Cuts at: 160 1032 4858 160 Size: 872 3826 1606 Fragments arranged by size: 3826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 2828 3629 1870 Size: 73 885 801 4545 Fragments arranged by size: 4545 885 801 73 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2188 2413 3597 3625 5080 Size: 325 217 627 401 225 1184 28 1455 Cuts at: 5080 618 Size: 1842 Fragments arranged by size: 1842 1455 1184 627 401 325 225 217 28 BanII G_rGCy'C Cuts at: 1391 2137 3020 3623 1391 Size: 746 883 603 4072 Fragments arranged by size: 4072 883 746 603 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2340 928 Size: 971 441 4892 Fragments arranged by size: 4892 971 441 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2045 2079 4495 4529 1903 Size: 34 108 34 2416 34 3678 Fragments arranged by size: 3678 2416 108 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 5406 4196 Size: 1210 5094 BclI T'GATC_A Cuts at: 1548 1548 Size: 6304 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4915 136 Size: 107 122 71 631 605 3243 1525 Fragments arranged by size: 3243 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3038 6299 3038 Size: 3261 3043 BlpI GC'TnA_GC Cuts at: 3721 3721 Size: 6304 Bme1580I G_kGCm'C Cuts at: 1790 2025 3578 4365 5521 1790 Size: 235 1553 787 1156 2573 Fragments arranged by size: 2573 1553 1156 787 235 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6304 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3596 4035 4993 Size: 416 53 83 399 24 2179 439 958 Cuts at: 4993 442 Size: 1753 Fragments arranged by size: 2179 1753 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3108 4964 1239 Size: 1869 1856 2579 Fragments arranged by size: 2579 1869 1856 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3135 4429 5294 5592 3135 Size: 1294 865 298 3847 Fragments arranged by size: 3847 1294 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2572 2977 4967 882 Size: 617 1073 405 1990 2219 Fragments arranged by size: 2219 1990 1073 617 405 BsaAI yAC'GTr Cuts at: 493 1204 4095 493 Size: 711 2891 2702 Fragments arranged by size: 2891 2702 711 BsaBI GATnn'nnATC Cuts at: 2976 6298 2976 Size: 3322 2982 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4111 4493 5128 275 Size: 53 83 186 936 2578 382 635 1451 Fragments arranged by size: 2578 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4737 5135 5409 5556 5967 4737 Size: 398 274 147 411 5074 Fragments arranged by size: 5074 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2498 2528 2710 2740 1178 Size: 30 165 30 1095 30 182 30 4742 Fragments arranged by size: 4742 1095 182 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2251 2254 2417 1186 Size: 1065 3 163 5073 Fragments arranged by size: 5073 1065 163 3 BseYI C'CCAG_C Cuts at: 2046 2553 2660 2994 5507 6003 2046 Size: 507 107 334 2513 496 2347 Fragments arranged by size: 2513 2347 507 496 334 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5731 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2530 2614 3754 4515 5543 1540 Size: 275 186 529 84 1140 761 1028 2301 Fragments arranged by size: 2301 1140 1028 761 529 275 186 84 BsiHKAI G_wGCw'C Cuts at: 3020 3578 4365 4450 5521 3020 Size: 558 787 85 1071 3803 Fragments arranged by size: 3803 1071 787 558 85 BsmI GAATG_Cn' Cuts at: 3092 3815 3908 3092 Size: 723 93 5488 Fragments arranged by size: 5488 723 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2137 3020 3578 3623 4365 4450 Size: 399 235 112 883 558 45 742 85 Cuts at: 4450 5521 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 883 742 558 399 235 112 85 45 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3659 844 Size: 2815 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4191 1394 Size: 429 2368 3507 Fragments arranged by size: 3507 2368 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4799 4973 65 Size: 1183 3551 174 1396 Fragments arranged by size: 3551 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2529 2609 4948 5135 1700 Size: 110 719 80 2339 187 2869 Fragments arranged by size: 2869 2339 719 187 110 80 BsrGI T'GTAC_A Cuts at: 96 2520 3396 96 Size: 2424 876 3004 Fragments arranged by size: 3004 2424 876 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 4364 5376 4364 Size: 1012 5292 BstAPI GCAn_nnn'nTGC Cuts at: 794 3305 794 Size: 2511 3793 BstBI TT'CG_AA Cuts at: 5108 5108 Size: 6304 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6304 BstXI CCAn_nnnn'nTGG Cuts at: 2129 2129 Size: 6304 BstYI r'GATC_y Cuts at: 1972 3038 3423 3634 4001 4386 4403 5855 5941 Size: 1066 385 211 367 385 17 1452 86 Cuts at: 5941 5953 6299 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1066 385 385 367 346 211 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2270 2418 2874 3237 513 Size: 552 939 266 148 456 363 3580 Fragments arranged by size: 3580 939 552 456 363 266 148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5565 Fragments arranged by size: 5565 683 56 BtsI GCAGTG_nn' Cuts at: 2115 2627 3820 4614 4634 2115 Size: 512 1193 794 20 3785 Fragments arranged by size: 3785 1193 794 512 20 ClaI AT'CG_AT Cuts at: 1554 2127 1554 Size: 573 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DraIII CAC_nnn'GTG Cuts at: 3125 3125 Size: 6304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5311 809 Size: 507 3995 1802 Fragments arranged by size: 3995 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2586 2611 3751 Size: 54 1425 50 275 18 756 25 1140 Cuts at: 3751 4640 6269 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1140 889 756 275 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2611 3751 1537 Size: 275 799 1140 4090 Fragments arranged by size: 4090 1140 799 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 2277 2295 2403 2947 3240 3258 4234 Size: 873 51 18 108 544 293 18 976 Cuts at: 4234 1353 Size: 3423 Fragments arranged by size: 3423 976 873 544 293 108 51 18 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4891 5265 5411 387 Size: 1064 138 3302 374 146 1280 Fragments arranged by size: 3302 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3018 3018 Size: 6304 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 3108 3502 4367 4964 5751 6019 Size: 393 669 807 394 865 597 787 268 Cuts at: 6019 1239 Size: 1524 Fragments arranged by size: 1524 865 807 787 669 597 394 393 268 EcoO109I rG'GnC_Cy Cuts at: 2038 2050 3155 2038 Size: 12 1105 5187 Fragments arranged by size: 5187 1105 12 EcoRI G'AATT_C Cuts at: 3250 3613 3991 3250 Size: 363 378 5563 Fragments arranged by size: 5563 378 363 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3326 3326 Size: 6304 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 5451 1678 Size: 3773 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 2789 2821 3159 Size: 32 135 32 803 32 382 32 338 Cuts at: 3159 3191 3415 3447 3476 3508 4945 4977 5019 Size: 32 224 32 29 32 1437 32 42 Cuts at: 5019 5051 1373 Size: 32 2626 Fragments arranged by size: 2626 1437 803 382 338 224 135 42 32 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 3648 3894 669 Size: 988 281 1710 246 3079 Fragments arranged by size: 3079 1710 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6304 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KpnI G_GTAC'C Cuts at: 3601 3629 3601 Size: 28 6276 MfeI C'AATT_G Cuts at: 3903 3903 Size: 6304 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2535 3045 5418 5602 1305 Size: 1230 510 2373 184 2007 Fragments arranged by size: 2373 2007 1230 510 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4482 Fragments arranged by size: 4482 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2127 4262 4621 4780 518 Size: 648 961 2135 359 159 2042 Fragments arranged by size: 2135 2042 961 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2272 2816 3239 3720 4397 5545 5790 1606 Size: 666 544 423 481 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 666 544 481 423 245 NaeI GCC'GGC Cuts at: 2611 2611 Size: 6304 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4813 Fragments arranged by size: 4813 939 552 NdeI CA'TA_TG Cuts at: 387 3379 387 Size: 2992 3312 NgoMIV G'CCGG_C Cuts at: 2609 2609 Size: 6304 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 3085 3590 3662 5207 1132 Size: 97 1856 505 72 1545 2229 Fragments arranged by size: 2229 1856 1545 505 97 72 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6304 PciI A'CATG_T Cuts at: 5203 5203 Size: 6304 PflMI CCAn_nnn'nTGG Cuts at: 1108 2933 1108 Size: 1825 4479 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2038 2050 2038 Size: 12 6292 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PstI C_TGCA'G Cuts at: 830 2475 3595 3656 830 Size: 1645 1120 61 3478 Fragments arranged by size: 3478 1645 1120 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 SacI G_AGCT'C Cuts at: 3020 3020 Size: 6304 SacII CC_GC'GG Cuts at: 2273 3240 2273 Size: 967 5337 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SanDI GG'GwC_CC Cuts at: 2050 2050 Size: 6304 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SfcI C'TryA_G Cuts at: 826 1045 2471 3591 3652 4787 5468 5659 826 Size: 219 1426 1120 61 1135 681 191 1471 Fragments arranged by size: 1471 1426 1135 1120 681 219 191 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 3631 1872 Size: 1759 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3150 4408 5309 5571 820 Size: 197 926 1207 1258 901 262 1553 Fragments arranged by size: 1553 1258 1207 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3590 3662 1132 Size: 2458 72 3774 Fragments arranged by size: 3774 2458 72 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6304 StyI C'CwwG_G Cuts at: 513 1065 2004 3743 513 Size: 552 939 1739 3074 Fragments arranged by size: 3074 1739 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2596 4331 4348 4501 4686 5131 325 Size: 1255 1016 1735 17 153 185 445 1498 Fragments arranged by size: 1735 1498 1255 1016 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 3396 4550 Size: 274 80 33 51 1443 543 876 1154 Cuts at: 4550 6262 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1154 876 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2710 2742 6264 6296 2710 Size: 32 3522 32 2718 Fragments arranged by size: 3522 2718 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6304 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2934 2934 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 3629 1870 Size: 1759 4545 XmnI GAAnn'nnTTC Cuts at: 4433 4433 Size: 6304 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4112 276 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI SrfI SwaI