Restriction map for
pFN21AB5901
(Linear) (Six-base) MAPSORT of: KIBB5901.seq Check: 4191 from: 1 to: 1500
KIBB5901.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 0 1012 1500
Size: 1012 488
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1500
Size: 1063 437
AflII C'TTAA_G
Cuts at: 0 868 1500
Size: 868 632
AhdI GACnn_n'nnGTC
Cuts at: 0 739 1500
Size: 739 761
AleI CACnn'nnGTG
Cuts at: 0 645 1057 1500
Size: 645 412 443
Fragments arranged by size:
645 443 412
AlwNI CAG_nnn'CTG
Cuts at: 0 763 1500
Size: 763 737
ApaI G_GGCC'C
Cuts at: 0 1036 1500
Size: 1036 464
ApoI r'AATT_y
Cuts at: 0 61 700 1128 1140 1458 1500
Size: 61 639 428 12 318 42
Fragments arranged by size:
639 428 318 61 42 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1478 1500
Size: 1478 22
BanI G'GyrC_C
Cuts at: 0 1401 1500
Size: 1401 99
BanII G_rGCy'C
Cuts at: 0 19 1036 1500
Size: 19 1017 464
Fragments arranged by size:
1017 464 19
BbvCI CC'TCA_GC
Cuts at: 0 469 1500
Size: 469 1031
BciVI GTATCCnnnnn_n'
Cuts at: 0 867 1500
Size: 867 633
BfrBI ATG'CAT
Cuts at: 0 539 1500
Size: 539 961
Bme1580I G_kGCm'C
Cuts at: 0 1036 1406 1500
Size: 1036 370 94
Fragments arranged by size:
1036 370 94
BmrI ACTGGGnnnn_n'
Cuts at: 0 1042 1500
Size: 1042 458
Bpu10I CC'TnA_GC
Cuts at: 0 469 1500
Size: 469 1031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1500
Size: 887 613
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 1500
Size: 43 1457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 1500
Size: 1385 115
BsiHKAI G_wGCw'C
Cuts at: 0 757 1500
Size: 757 743
BsmI GAATG_Cn'
Cuts at: 0 1228 1500
Size: 1228 272
Bsp1286I G_dGCh'C
Cuts at: 0 19 757 1036 1406 1500
Size: 19 738 279 370 94
Fragments arranged by size:
738 370 279 94 19
BsrBI CCG'CTC
Cuts at: 0 72 1500
Size: 72 1428
BsrDI GCAATG_nn'
Cuts at: 0 539 1112 1356 1500
Size: 539 573 244 144
Fragments arranged by size:
573 539 244 144
BsrGI T'GTAC_A
Cuts at: 0 1181 1500
Size: 1181 319
BstAPI GCAn_nnn'nTGC
Cuts at: 0 163 1500
Size: 163 1337
BstZ17I GTA'TAC
Cuts at: 0 1013 1500
Size: 1013 487
Bsu36I CC'TnA_GG
Cuts at: 0 546 1500
Size: 546 954
BtgI C'CryG_G
Cuts at: 0 23 1348 1500
Size: 23 1325 152
Fragments arranged by size:
1325 152 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 35 281 1500
Size: 35 246 1219
Fragments arranged by size:
1219 246 35
BtsI GCAGTG_nn'
Cuts at: 0 237 652 1368 1500
Size: 237 415 716 132
Fragments arranged by size:
716 415 237 132
DraI TTT'AAA
Cuts at: 0 119 1500
Size: 119 1381
EaeI y'GGCC_r
Cuts at: 0 1351 1500
Size: 1351 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1500
Size: 1063 437
EcoO109I rG'GnC_Cy
Cuts at: 0 1033 1500
Size: 1033 467
EcoRI G'AATT_C
Cuts at: 0 700 1500
Size: 700 800
FspI TGC'GCA
Cuts at: 0 41 1500
Size: 41 1459
HaeII r_GCGC'y
Cuts at: 0 977 1500
Size: 977 523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 725 757 823 855 1500
Size: 670 32 23 32 66 32 645
Fragments arranged by size:
670 645 66 32 32 32 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1500
Size: 1222 278
MslI CAynn'nnrTG
Cuts at: 0 460 645 854 1057 1361 1500
Size: 460 185 209 203 304 139
Fragments arranged by size:
460 304 209 203 185 139
MspA1I CmG'CkG
Cuts at: 0 25 1350 1500
Size: 25 1325 150
Fragments arranged by size:
1325 150 25
NdeI CA'TA_TG
Cuts at: 0 177 1500
Size: 177 1323
NsiI A_TGCA'T
Cuts at: 0 541 1500
Size: 541 959
NspI r_CATG'y
Cuts at: 0 46 613 1500
Size: 46 567 887
Fragments arranged by size:
887 567 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 55 1500
Size: 55 1445
PfoI T'CCnGG_A
Cuts at: 0 979 1500
Size: 979 521
PspOMI G'GGCC_C
Cuts at: 0 1032 1500
Size: 1032 468
SacII CC_GC'GG
Cuts at: 0 26 1351 1500
Size: 26 1325 149
Fragments arranged by size:
1325 149 26
ScaI AGT'ACT
Cuts at: 0 143 1500
Size: 143 1357
SfcI C'TryA_G
Cuts at: 0 1481 1500
Size: 1481 19
SmlI C'TyrA_G
Cuts at: 0 868 902 1500
Size: 868 34 598
Fragments arranged by size:
868 598 34
SphI G_CATG'C
Cuts at: 0 46 1500
Size: 46 1454
TatI w'GTAC_w
Cuts at: 0 141 1181 1500
Size: 141 1040 319
Fragments arranged by size:
1040 319 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 51 83 542 574 921 953 1500
Size: 51 32 459 32 347 32 547
Fragments arranged by size:
547 459 347 51 32 32 32
Enzymes that do cut:
AccI AcuI AflII AhdI AleI AlwNI ApaI ApoI
BaeI BanI BanII BbvCI BciVI BfrBI Bme1580I BmrI
Bpu10I BpuEI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrBI
BsrDI BsrGI BstAPI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI EaeI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
MmeI MslI MspA1I NdeI NsiI NspI PflMI PfoI
PspOMI SacII ScaI SfcI SmlI SphI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AlfI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BbsI BcgI BclI BglI BglII BlpI BmgBI
BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrFI BssHII BssSI BstBI BstEII BstXI BstYI ClaI
CspCI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI StyI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI