Restriction map for
pFN21AB5782
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BbvCI BciVI BclI BfrI
BglII BlnI BsaBI BsiWI BsmBI
BspDI BspLU11I BsrBI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI DraI
DrdI Eam1105I EarI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MfeI
MluI MluNI MscI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspI PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AatII 1567 GACGTC GACGT^C
AccIII 679 TCCGGA T^CCGGA
AcsI 892 GAATTC [GA]^AATT[CT]
Alw44I 72 GTGCAC G^TGCAC
AocI 426 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1625 AGCGCT AGC^GCT
ApaLI 72 GTGCAC G^TGCAC
ApoI 892 GAATTC [GA]^AATT[CT]
AspHI 72 GTGCAC G[AT]GC[AT]^C
AspI 1531 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 165 GGATCC G^GATCC
BbeI 826 GGCGCC GGCGC^C
BbrPI 616 CACGTG CAC^GTG
BfaI 445 CTAG C^TAG
BlpI 1709 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1709 GCTCAGC GC^T[ACGT]AGC
BsaAI 616 CACGTG [CT]AC^GT[GA]
BsaI 1361 GAGACC GGTCTC(1/5)
BsaWI 679 TCCGGA [AT]^CCGG[AT]
BseAI 679 TCCGGA T^CCGGA
BsiHKAI 72 GTGCAC G[AT]GC[AT]^C
BsmAI 1361 GAGAC GTCTC(1/5)
Bsp1407I 875 TGTACA T^GTACA
BspEI 679 TCCGGA T^CCGGA
BsrGI 875 TGTACA T^GTACA
BstBI 1900 TTCGAA TT^CGAA
BstEII 963 GGTGACC G^GT[ACGT]ACC
BstPI 963 GGTGACC G^GT[ACGT]ACC
BstYI 165 GGATCC [GA]^GATC[CT]
Bsu36I 426 CCTCAGG CC^T[ACGT]AGG
CelII 1709 GCTCAGC GC^T[ACGT]AGC
Eco47III 1625 AGCGCT AGC^GCT
Eco81I 426 CCTCAGG CC^T[ACGT]AGG
EcoNI 60 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 963 GGTGACC G^GT[ACGT]ACC
EcoRI 892 GAATTC G^AATTC
EcoT22I 105 ATGCAT ATGCA^T
KasI 826 GGCGCC G^GCGCC
KspI 1753 CCGCGG CCGC^GG
MaeI 445 CTAG C^TAG
MflI 165 GGATCC [GA]^GATC[CT]
MroI 679 TCCGGA T^CCGGA
MseI 1067 TTAA T^TAA
MslI 2058 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 826 GGCGCC GG^CGCC
NcoI 1377 CCATGG C^CATGG
NsiI 105 ATGCAT ATGCA^T
NspV 1900 TTCGAA TT^CGAA
PflFI 1531 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1470 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 616 CACGTG CAC^GTG
PmlI 616 CACGTG CAC^GTG
PshAI 654 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1753 CCGCGG CCGC^GG
ScaI 1595 AGTACT AGT^ACT
SexAI 130 ACCTGGT A^CC[AT]GGT
SfoI 826 GGCGCC GGC^GCC
SfuI 1900 TTCGAA TT^CGAA
SmlI 2227 CTCAAG C^T[CT][GA]AG
SspBI 875 TGTACA T^GTACA
Tru9I 1067 TTAA T^TAA
Tsp509I 893 AATT ^AATT
Tth111I 1531 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1470 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 165 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BseRI 1089 CTCCTC GAGGAG(10/8)
882 GAGGAG
BsiEI 489 CGGCCG CG[GA][CT]^CG
849 CGGCCG
BspHI 206 TCATGA T^CATGA
2282 TCATGA
BsrDI 1049 GCAATG GCAATG(2/0)
1160 GCAATG
BsrFI 1340 GCCGGT [GA]^CCGG[CT]
1781 GCCGGT
BstXI 968 CCAGTAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1046 CCAGCAATGTGG
Cfr10I 1340 GCCGGT [GA]^CCGG[CT]
1781 GCCGGT
EagI 489 CGGCCG C^GGCCG
849 CGGCCG
EclXI 489 CGGCCG C^GGCCG
849 CGGCCG
Eco52I 489 CGGCCG C^GGCCG
849 CGGCCG
EcoT14I 365 CCAAGG C^C[AT][AT]GG
1377 CCATGG
RcaI 206 TCATGA T^CATGA
2282 TCATGA
SmaI 1653 CCCGGG CCC^GGG
1695 CCCGGG
StyI 365 CCAAGG C^C[AT][AT]GG
1377 CCATGG
XcmI 1013 CCAAGTCAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1094 CCAGGGTCTTCCTGG
XmaCI 1653 CCCGGG C^CCGGG
1695 CCCGGG
XmaI 1653 CCCGGG C^CCGGG
1695 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BanI BanII BbsI BbvI BcgI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaJI BsgI BsiYI BslI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I