Restriction map for
pFN21AB5782
(Linear) (Six-base) MAPSORT of: KIBB5782.seq Check: 1260 from: 1 to: 2307
KIBB5782.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1400 2307
Size: 1400 907
AatII G_ACGT'C
Cuts at: 0 1571 2307
Size: 1571 736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 643 1915 2307
Size: 594 49 1272 392
Fragments arranged by size:
1272 594 392 49
AfeI AGC'GCT
Cuts at: 0 1627 2307
Size: 1627 680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2170 2307
Size: 2170 137
AlwNI CAG_nnn'CTG
Cuts at: 0 95 144 1373 1476 1551 1982 2307
Size: 95 49 1229 103 75 431 325
Fragments arranged by size:
1229 431 325 103 95 75 49
ApaI G_GGCC'C
Cuts at: 0 122 468 1912 2307
Size: 122 346 1444 395
Fragments arranged by size:
1444 395 346 122
ApaLI G'TGCA_C
Cuts at: 0 72 2307
Size: 72 2235
ApoI r'AATT_y
Cuts at: 0 892 2307
Size: 892 1415
AvaI C'yCGr_G
Cuts at: 0 49 697 1653 1695 2307
Size: 49 648 956 42 612
Fragments arranged by size:
956 648 612 49 42
BamHI G'GATC_C
Cuts at: 0 165 2307
Size: 165 2142
BanI G'GyrC_C
Cuts at: 0 134 826 1343 1784 2119 2307
Size: 134 692 517 441 335 188
Fragments arranged by size:
692 517 441 335 188 134
BanII G_rGCy'C
Cuts at: 0 122 468 1667 1711 1912 2307
Size: 122 346 1199 44 201 395
Fragments arranged by size:
1199 395 346 201 122 44
BbeI G_GCGC'C
Cuts at: 0 830 2307
Size: 830 1477
BbsI GAAGACnn'nnnn_
Cuts at: 0 272 988 1092 2307
Size: 272 716 104 1215
Fragments arranged by size:
1215 716 272 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 483 517 551 585 876 910 2307
Size: 483 34 34 34 291 34 1397
Fragments arranged by size:
1397 483 291 34 34 34 34
BfrBI ATG'CAT
Cuts at: 0 107 2307
Size: 107 2200
BglI GCCn_nnn'nGGC
Cuts at: 0 497 1697 1814 2307
Size: 497 1200 117 493
Fragments arranged by size:
1200 497 493 117
BlpI GC'TnA_GC
Cuts at: 0 1710 2307
Size: 1710 597
Bme1580I G_kGCm'C
Cuts at: 0 76 122 468 1846 1912 2122 2307
Size: 76 46 346 1378 66 210 185
Fragments arranged by size:
1378 346 210 185 76 66 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 1323 2229 2307
Size: 1323 906 78
Fragments arranged by size:
1323 906 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2013 2307
Size: 2013 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1029 1077 2047 2307
Size: 883 146 48 970 260
Fragments arranged by size:
970 883 260 146 48
Bpu10I CC'TnA_GC
Cuts at: 0 1518 2185 2307
Size: 1518 667 122
Fragments arranged by size:
1518 667 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2212 2307
Size: 2212 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 1355 2307
Size: 1355 952
BsaAI yAC'GTr
Cuts at: 0 618 2307
Size: 618 1689
BsaHI Gr'CG_yC
Cuts at: 0 713 827 1568 2307
Size: 713 114 741 739
Fragments arranged by size:
741 739 713 114
BsaWI w'CCGG_w
Cuts at: 0 679 2307
Size: 679 1628
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 1258 1288 1489 1519 2307
Size: 574 30 654 30 201 30 788
Fragments arranged by size:
788 654 574 201 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 897 1080 2307
Size: 897 183 1227
Fragments arranged by size:
1227 897 183
BseYI C'CCAG_C
Cuts at: 0 3 822 1290 1524 2009 2307
Size: 3 819 468 234 485 298
Fragments arranged by size:
819 485 468 298 234 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 1228 1336 2307
Size: 111 1117 108 971
Fragments arranged by size:
1117 971 111 108
BsiEI CG_ry'CG
Cuts at: 0 492 852 2307
Size: 492 360 1455
Fragments arranged by size:
1455 492 360
BsiHKAI G_wGCw'C
Cuts at: 0 76 2307
Size: 76 2231
BsmI GAATG_Cn'
Cuts at: 0 109 1077 2147 2307
Size: 109 968 1070 160
Fragments arranged by size:
1070 968 160 109
Bsp1286I G_dGCh'C
Cuts at: 0 76 122 468 1667 1711 1846 1912 2122
Size: 76 46 346 1199 44 135 66 210
Cuts at: 2122 2307
Size: 185
Fragments arranged by size:
1199 346 210 185 135 76 66 46
44
BspEI T'CCGG_A
Cuts at: 0 679 2307
Size: 679 1628
BspHI T'CATG_A
Cuts at: 0 206 2282 2307
Size: 206 2076 25
Fragments arranged by size:
2076 206 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 326 464 714 1308 1400 1990 2307
Size: 326 138 250 594 92 590 317
Fragments arranged by size:
594 590 326 317 250 138 92
BsrDI GCAATG_nn'
Cuts at: 0 1056 1167 2307
Size: 1056 111 1140
Fragments arranged by size:
1140 1056 111
BsrFI r'CCGG_y
Cuts at: 0 1340 1781 2307
Size: 1340 441 526
Fragments arranged by size:
1340 526 441
BsrGI T'GTAC_A
Cuts at: 0 875 2307
Size: 875 1432
BstAPI GCAn_nnn'nTGC
Cuts at: 0 134 1373 1799 1982 2307
Size: 134 1239 426 183 325
Fragments arranged by size:
1239 426 325 183 134
BstBI TT'CG_AA
Cuts at: 0 1901 2307
Size: 1901 406
BstEII G'GTnAC_C
Cuts at: 0 963 2307
Size: 963 1344
BstXI CCAn_nnnn'nTGG
Cuts at: 0 975 1053 2307
Size: 975 78 1254
Fragments arranged by size:
1254 975 78
BstYI r'GATC_y
Cuts at: 0 165 2307
Size: 165 2142
Bsu36I CC'TnA_GG
Cuts at: 0 427 2307
Size: 427 1880
BtgI C'CryG_G
Cuts at: 0 1022 1377 1753 2123 2307
Size: 1022 355 376 370 184
Fragments arranged by size:
1022 376 370 355 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2085 2307
Size: 2085 222
BtsI GCAGTG_nn'
Cuts at: 0 19 1552 2307
Size: 19 1533 755
Fragments arranged by size:
1533 755 19
DraIII CAC_nnn'GTG
Cuts at: 0 134 1256 1864 2307
Size: 134 1122 608 443
Fragments arranged by size:
1122 608 443 134
EaeI y'GGCC_r
Cuts at: 0 193 489 849 1247 1635 1779 2307
Size: 193 296 360 398 388 144 528
Fragments arranged by size:
528 398 388 360 296 193 144
EagI C'GGCC_G
Cuts at: 0 489 849 2307
Size: 489 360 1458
Fragments arranged by size:
1458 489 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 643 883 1029 1077 1915 2047 2307
Size: 594 49 240 146 48 838 132 260
Fragments arranged by size:
838 594 260 240 146 132 49 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 64 2307
Size: 64 2243
EcoO109I rG'GnC_Cy
Cuts at: 0 118 465 506 1320 1908 1909 1979 2092
Size: 118 347 41 814 588 1 70 113
Cuts at: 2092 2307
Size: 215
Fragments arranged by size:
814 588 347 215 118 113 70 41
1
EcoRI G'AATT_C
Cuts at: 0 892 2307
Size: 892 1415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 165 328 1407 1929 2307
Size: 165 163 1079 522 378
Fragments arranged by size:
1079 522 378 165 163
HaeII r_GCGC'y
Cuts at: 0 830 1291 1629 2211 2270 2307
Size: 830 461 338 582 59 37
Fragments arranged by size:
830 582 461 338 59 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 889 921 1489 1521 2307
Size: 157 32 700 32 568 32 786
Fragments arranged by size:
786 700 568 157 32 32 32
KasI G'GCGC_C
Cuts at: 0 826 2307
Size: 826 1481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 2307
Size: 665 1642
MslI CAynn'nnrTG
Cuts at: 0 2062 2307
Size: 2062 245
MspA1I CmG'CkG
Cuts at: 0 95 201 291 672 854 918 1688 1755
Size: 95 106 90 381 182 64 770 67
Cuts at: 1755 1790 1824 1953 2307
Size: 35 34 129 354
Fragments arranged by size:
770 381 354 182 129 106 95 90
67 64 35 34
NarI GG'CG_CC
Cuts at: 0 827 2307
Size: 827 1480
NcoI C'CATG_G
Cuts at: 0 1377 2307
Size: 1377 930
NsiI A_TGCA'T
Cuts at: 0 109 2307
Size: 109 2198
PflMI CCAn_nnn'nTGG
Cuts at: 0 1476 2307
Size: 1476 831
PfoI T'CCnGG_A
Cuts at: 0 1103 1262 2195 2307
Size: 1103 159 933 112
Fragments arranged by size:
1103 933 159 112
PmlI CAC'GTG
Cuts at: 0 618 2307
Size: 618 1689
PpuMI rG'GwC_Cy
Cuts at: 0 506 1320 1979 2307
Size: 506 814 659 328
Fragments arranged by size:
814 659 506 328
PshAI GACnn'nnGTC
Cuts at: 0 658 2307
Size: 658 1649
PspOMI G'GGCC_C
Cuts at: 0 118 464 1908 2307
Size: 118 346 1444 399
Fragments arranged by size:
1444 399 346 118
PstI C_TGCA'G
Cuts at: 0 1159 1411 1930 1952 2307
Size: 1159 252 519 22 355
Fragments arranged by size:
1159 519 355 252 22
PvuII CAG'CTG
Cuts at: 0 95 201 291 672 918 1824 1953 2307
Size: 95 106 90 381 246 906 129 354
Fragments arranged by size:
906 381 354 246 129 106 95 90
SacII CC_GC'GG
Cuts at: 0 1756 2307
Size: 1756 551
ScaI AGT'ACT
Cuts at: 0 1597 2307
Size: 1597 710
SexAI A'CCwGG_T
Cuts at: 0 130 2307
Size: 130 2177
SfcI C'TryA_G
Cuts at: 0 1155 1407 1926 1948 2307
Size: 1155 252 519 22 359
Fragments arranged by size:
1155 519 359 252 22
SfoI GGC'GCC
Cuts at: 0 828 2307
Size: 828 1479
SmaI CCC'GGG
Cuts at: 0 1655 1697 2307
Size: 1655 42 610
Fragments arranged by size:
1655 610 42
SmlI C'TyrA_G
Cuts at: 0 2227 2307
Size: 2227 80
StyI C'CwwG_G
Cuts at: 0 365 1377 2307
Size: 365 1012 930
Fragments arranged by size:
1012 930 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 670 2001 2307
Size: 670 1331 306
Fragments arranged by size:
1331 670 306
TatI w'GTAC_w
Cuts at: 0 386 875 1595 1871 2201 2307
Size: 386 489 720 276 330 106
Fragments arranged by size:
720 489 386 330 276 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 1425 1457 2307
Size: 574 32 819 32 850
Fragments arranged by size:
850 819 574 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1534 2307
Size: 1534 773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1020 1101 2307
Size: 1020 81 1206
Fragments arranged by size:
1206 1020 81
XmaI C'CCGG_G
Cuts at: 0 1653 1695 2307
Size: 1653 42 612
Fragments arranged by size:
1653 612 42
ZraI GAC'GTC
Cuts at: 0 1569 2307
Size: 1569 738
Enzymes that do cut:
AarI AatII AcuI AfeI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI MmeI
MslI MspA1I NarI NcoI NsiI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacII ScaI SexAI
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI AscI AseI AsiSI AvrII BaeI BbvCI BciVI
BclI BglII BmgBI BmtI BsaBI BsiWI BsmBI BsrBI
BssHII BssSI BstZ17I ClaI CspCI DraI DrdI EarI
EciI EcoICRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI XmnI