(Circular) (Six-base) MAPSORT of: pFN21AB5874.seq Check: 7788 from: 1 to: 6187 pFN21AB5874 6187 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2514 2844 4250 5634 5902 1632 Size: 491 391 330 1406 1384 268 1917 Fragments arranged by size: 1917 1406 1384 491 391 330 268 AfeI AGC'GCT Cuts at: 2063 2788 2063 Size: 725 5462 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AlwNI CAG_nnn'CTG Cuts at: 3930 5502 3930 Size: 1572 4615 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 2332 2441 3496 3722 3874 5983 1493 Size: 839 109 1055 226 152 2109 1697 Fragments arranged by size: 2109 1697 1055 839 226 152 109 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 2043 3512 1870 Size: 73 100 1469 4545 Fragments arranged by size: 4545 1469 100 73 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3308 3508 4963 618 Size: 325 217 627 1521 200 1455 1842 Fragments arranged by size: 1842 1521 1455 627 325 217 200 BanII G_rGCy'C Cuts at: 1391 2681 2753 3506 1391 Size: 1290 72 753 4072 Fragments arranged by size: 4072 1290 753 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2532 3201 928 Size: 971 633 669 3914 Fragments arranged by size: 3914 971 669 633 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4378 4412 1903 Size: 34 50 34 2357 34 3678 Fragments arranged by size: 3678 2357 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2628 4798 136 Size: 107 122 71 631 605 956 2170 1525 Fragments arranged by size: 2170 1525 956 631 605 122 107 71 BglII A'GATC_T Cuts at: 2660 3218 3464 6182 2660 Size: 558 246 2718 2665 Fragments arranged by size: 2718 2665 558 246 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 4248 5404 1790 Size: 2458 1156 2573 Fragments arranged by size: 2573 2458 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3205 3918 4876 Size: 416 53 83 399 24 1788 713 958 Cuts at: 4876 442 Size: 1753 Fragments arranged by size: 1788 1753 958 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2679 2679 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2545 3367 4847 1239 Size: 872 434 822 1480 2579 Fragments arranged by size: 2579 1480 872 822 434 Bpu10I CC'TnA_GC Cuts at: 2030 2030 Size: 6187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3463 4312 5177 5475 3463 Size: 849 865 298 4175 Fragments arranged by size: 4175 865 849 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2589 3314 4850 882 Size: 617 1090 725 1536 2219 Fragments arranged by size: 2219 1536 1090 725 617 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 3223 3463 6181 3223 Size: 240 2718 3229 Fragments arranged by size: 3229 2718 240 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2548 3994 4376 5011 Size: 53 83 186 936 1015 1446 382 635 Cuts at: 5011 275 Size: 1451 Fragments arranged by size: 1451 1446 1015 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2321 2351 2701 2731 2899 Size: 30 165 30 918 30 350 30 168 Cuts at: 2899 2929 3311 3341 1178 Size: 30 382 30 4024 Fragments arranged by size: 4024 918 382 350 168 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2938 2948 2951 3104 3145 3303 1186 Size: 1752 10 3 153 41 158 4070 Fragments arranged by size: 4070 1752 158 153 41 10 3 BseYI C'CCAG_C Cuts at: 2753 5390 5886 2753 Size: 2637 496 3054 Fragments arranged by size: 3054 2637 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2368 1163 Size: 573 632 4982 Fragments arranged by size: 4982 632 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2026 3637 4398 5426 1540 Size: 275 186 25 1611 761 1028 2301 Fragments arranged by size: 2301 1611 1028 761 275 186 25 BsiHKAI G_wGCw'C Cuts at: 4248 4333 5404 4248 Size: 85 1071 5031 Fragments arranged by size: 5031 1071 85 BsmI GAATG_Cn' Cuts at: 3307 3698 3791 3307 Size: 391 93 5703 Fragments arranged by size: 5703 391 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2681 2753 3506 4248 4333 5404 1391 Size: 399 891 72 753 742 85 1071 2174 Fragments arranged by size: 2174 1071 891 753 742 399 85 72 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 4076 5806 4076 Size: 1730 4457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3542 844 Size: 2698 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2685 3207 3231 3247 4074 1394 Size: 429 862 522 24 16 827 3507 Fragments arranged by size: 3507 862 827 522 429 24 16 BsrDI GCAATG_nn' Cuts at: 65 1248 4682 4856 65 Size: 1183 3434 174 1396 Fragments arranged by size: 3434 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2550 4831 5018 1700 Size: 110 740 2281 187 2869 Fragments arranged by size: 2869 2281 740 187 110 BsrGI T'GTAC_A Cuts at: 96 2016 96 Size: 1920 4267 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 4247 5259 4247 Size: 1012 5175 BstAPI GCAn_nnn'nTGC Cuts at: 794 2153 794 Size: 1359 4828 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstXI CCAn_nnnn'nTGG Cuts at: 2789 2789 Size: 6187 BstYI r'GATC_y Cuts at: 1972 2085 2098 2660 2832 3218 3338 3428 3464 Size: 113 13 562 172 386 120 90 36 Cuts at: 3464 3517 3884 4269 4286 5738 5824 5836 6182 Size: 53 367 385 17 1452 86 12 346 Cuts at: 6182 1972 Size: 1977 Fragments arranged by size: 1977 1452 562 386 385 367 346 172 120 113 90 86 53 36 17 13 12 BtgI C'CryG_G Cuts at: 513 1065 2009 2215 2222 513 Size: 552 944 206 7 4478 Fragments arranged by size: 4478 944 552 206 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2067 487 Size: 683 56 841 4607 Fragments arranged by size: 4607 841 683 56 BtsI GCAGTG_nn' Cuts at: 2260 3703 4497 4517 2260 Size: 1443 794 20 3930 Fragments arranged by size: 3930 1443 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2278 3634 Size: 54 1425 50 275 18 193 255 1356 Cuts at: 3634 4523 6152 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1356 889 275 255 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2023 3634 1537 Size: 275 211 1611 4090 Fragments arranged by size: 4090 1611 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 2820 2972 3071 4117 1353 Size: 1467 152 99 1046 3423 Fragments arranged by size: 3423 1467 1046 152 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2975 4774 5148 5294 387 Size: 1064 138 1386 1799 374 146 1280 Fragments arranged by size: 1799 1386 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2123 2514 2545 2844 3367 4250 Size: 393 479 12 391 31 299 523 883 Cuts at: 4250 4847 5634 5902 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 883 787 597 523 479 393 391 299 268 31 12 EcoO109I rG'GnC_Cy Cuts at: 3471 3471 Size: 6187 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 2065 2790 5334 1678 Size: 387 725 2544 2531 Fragments arranged by size: 2544 2531 725 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 2701 2733 2857 Size: 32 135 32 749 32 348 32 124 Cuts at: 2857 2887 2889 2899 2919 2931 3085 3117 3268 Size: 30 2 10 20 12 154 32 151 Cuts at: 3268 3300 3311 3343 4828 4860 4902 4934 1373 Size: 32 11 32 1485 32 42 32 2626 Fragments arranged by size: 2626 1485 749 348 154 151 135 124 42 32 32 32 32 32 32 32 32 32 30 20 12 11 10 2 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6187 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2463 2699 2885 2975 5301 5485 1305 Size: 1158 236 186 90 2326 184 2007 Fragments arranged by size: 2326 2007 1158 236 186 184 90 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4365 Fragments arranged by size: 4365 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4145 4504 4663 518 Size: 648 2979 359 159 2042 Fragments arranged by size: 2979 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2217 2352 3603 4280 5428 5673 1606 Size: 405 206 135 1251 677 1148 245 2120 Fragments arranged by size: 2120 1251 1148 677 405 245 206 135 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5635 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 2213 3257 3545 5090 1132 Size: 97 984 1044 288 1545 2229 Fragments arranged by size: 2229 1545 1044 984 288 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6187 PfoI T'CCnGG_A Cuts at: 2088 2687 2088 Size: 599 5588 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3471 3471 Size: 6187 PshAI GACnn'nnGTC Cuts at: 1613 2551 1613 Size: 938 5249 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PstI C_TGCA'G Cuts at: 830 3539 830 Size: 2709 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2352 2352 Size: 6187 SacII CC_GC'GG Cuts at: 2012 2218 2012 Size: 206 5981 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SapI GCTCTTCn'nnn_ Cuts at: 2972 2972 Size: 6187 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 3535 4670 5351 5542 826 Size: 219 2490 1135 681 191 1471 Fragments arranged by size: 2490 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 2045 3514 1872 Size: 173 1469 4545 Fragments arranged by size: 4545 1469 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4291 5192 5454 820 Size: 197 926 1535 813 901 262 1553 Fragments arranged by size: 1553 1535 926 901 813 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 2213 3545 1132 Size: 1081 1332 3774 Fragments arranged by size: 3774 1332 1081 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 3626 513 Size: 552 2561 3074 Fragments arranged by size: 3074 2561 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2655 3405 4214 4231 4384 4569 5014 Size: 1255 1075 750 809 17 153 185 445 Cuts at: 5014 325 Size: 1498 Fragments arranged by size: 1498 1255 1075 809 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2016 2313 4433 Size: 274 80 33 51 1443 39 297 2120 Cuts at: 4433 6145 96 Size: 1712 138 Fragments arranged by size: 2120 1712 1443 297 274 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3467 3499 6147 6179 3467 Size: 32 2648 32 3475 Fragments arranged by size: 3475 2648 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2849 3325 1316 Size: 1533 476 4178 Fragments arranged by size: 4178 1533 476 XbaI T'CTAG_A Cuts at: 3425 3461 3523 3425 Size: 36 62 6089 Fragments arranged by size: 6089 62 36 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 2043 3512 1870 Size: 173 1469 4545 Fragments arranged by size: 4545 1469 173 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI