Restriction map for
pFN21AB5874
(Linear) (Six-base) MAPSORT of: KIBB5874.seq Check: 9669 from: 1 to: 1482
KIBB5874.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 509 839 1482
Size: 118 391 330 643
Fragments arranged by size:
643 391 330 118
AfeI AGC'GCT
Cuts at: 0 58 783 1482
Size: 58 725 699
Fragments arranged by size:
725 699 58
ApoI r'AATT_y
Cuts at: 0 327 436 1482
Size: 327 109 1046
Fragments arranged by size:
1046 327 109
AvaI C'yCGr_G
Cuts at: 0 38 1482
Size: 38 1444
BanI G'GyrC_C
Cuts at: 0 1303 1482
Size: 1303 179
BanII G_rGCy'C
Cuts at: 0 676 748 1482
Size: 676 72 734
Fragments arranged by size:
734 676 72
BbsI GAAGACnn'nnnn_
Cuts at: 0 527 1196 1482
Size: 527 669 286
Fragments arranged by size:
669 527 286
BglI GCCn_nnn'nGGC
Cuts at: 0 623 1482
Size: 623 859
BglII A'GATC_T
Cuts at: 0 655 1213 1459 1482
Size: 655 558 246 23
Fragments arranged by size:
655 558 246 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 1200 1482
Size: 1200 282
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 674 1482
Size: 674 808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 540 1362 1482
Size: 106 434 822 120
Fragments arranged by size:
822 434 120 106
Bpu10I CC'TnA_GC
Cuts at: 0 25 1482
Size: 25 1457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1458 1482
Size: 1458 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 584 1309 1482
Size: 584 725 173
Fragments arranged by size:
725 584 173
BsaBI GATnn'nnATC
Cuts at: 0 1218 1458 1482
Size: 1218 240 24
Fragments arranged by size:
1218 240 24
BsaHI Gr'CG_yC
Cuts at: 0 543 1482
Size: 543 939
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 696 726 894 924 1306 1336
Size: 316 30 350 30 168 30 382 30
Cuts at: 1336 1482
Size: 146
Fragments arranged by size:
382 350 316 168 146 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 933 943 946 1099 1140 1298 1482
Size: 933 10 3 153 41 158 184
Fragments arranged by size:
933 184 158 153 41 10 3
BseYI C'CCAG_C
Cuts at: 0 748 1482
Size: 748 734
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 1482
Size: 363 1119
BsiEI CG_ry'CG
Cuts at: 0 21 1482
Size: 21 1461
BsmI GAATG_Cn'
Cuts at: 0 1302 1482
Size: 1302 180
Bsp1286I G_dGCh'C
Cuts at: 0 676 748 1482
Size: 676 72 734
Fragments arranged by size:
734 676 72
BsrBI CCG'CTC
Cuts at: 0 680 1202 1226 1242 1482
Size: 680 522 24 16 240
Fragments arranged by size:
680 522 240 24 16
BsrFI r'CCGG_y
Cuts at: 0 545 1482
Size: 545 937
BsrGI T'GTAC_A
Cuts at: 0 11 1482
Size: 11 1471
BstAPI GCAn_nnn'nTGC
Cuts at: 0 148 1482
Size: 148 1334
BstXI CCAn_nnnn'nTGG
Cuts at: 0 784 1482
Size: 784 698
BstYI r'GATC_y
Cuts at: 0 80 93 655 827 1213 1333 1423 1459
Size: 80 13 562 172 386 120 90 36
Cuts at: 1459 1482
Size: 23
Fragments arranged by size:
562 386 172 120 90 80 36 23
13
BtgI C'CryG_G
Cuts at: 0 4 210 217 1482
Size: 4 206 7 1265
Fragments arranged by size:
1265 206 7 4
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 62 1482
Size: 62 1420
BtsI GCAGTG_nn'
Cuts at: 0 255 1482
Size: 255 1227
EaeI y'GGCC_r
Cuts at: 0 18 273 1482
Size: 18 255 1209
Fragments arranged by size:
1209 255 18
EagI C'GGCC_G
Cuts at: 0 18 1482
Size: 18 1464
EarI CTCTTCn'nnn_
Cuts at: 0 815 967 1066 1482
Size: 815 152 99 416
Fragments arranged by size:
815 416 152 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 970 1482
Size: 970 512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 118 509 540 839 1362 1482
Size: 106 12 391 31 299 523 120
Fragments arranged by size:
523 391 299 120 106 31 12
EcoO109I rG'GnC_Cy
Cuts at: 0 1466 1482
Size: 1466 16
HaeII r_GCGC'y
Cuts at: 0 60 785 1482
Size: 60 725 697
Fragments arranged by size:
725 697 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 696 728 852 882 884 894
Size: 316 32 348 32 124 30 2 10
Cuts at: 894 914 926 1080 1112 1263 1295 1306 1338
Size: 20 12 154 32 151 32 11 32
Cuts at: 1338 1482
Size: 144
Fragments arranged by size:
348 316 154 151 144 124 32 32
32 32 32 30 20 12 11 10
2
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 694 880 970 1482
Size: 458 236 186 90 512
Fragments arranged by size:
512 458 236 186 90
MspA1I CmG'CkG
Cuts at: 0 6 212 347 1482
Size: 6 206 135 1135
Fragments arranged by size:
1135 206 135 6
NspI r_CATG'y
Cuts at: 0 208 1252 1482
Size: 208 1044 230
Fragments arranged by size:
1044 230 208
PfoI T'CCnGG_A
Cuts at: 0 83 682 1482
Size: 83 599 800
Fragments arranged by size:
800 599 83
PpuMI rG'GwC_Cy
Cuts at: 0 1466 1482
Size: 1466 16
PshAI GACnn'nnGTC
Cuts at: 0 546 1482
Size: 546 936
PvuII CAG'CTG
Cuts at: 0 347 1482
Size: 347 1135
SacII CC_GC'GG
Cuts at: 0 7 213 1482
Size: 7 206 1269
Fragments arranged by size:
1269 206 7
SapI GCTCTTCn'nnn_
Cuts at: 0 967 1482
Size: 967 515
SmaI CCC'GGG
Cuts at: 0 40 1482
Size: 40 1442
SmlI C'TyrA_G
Cuts at: 0 1473 1482
Size: 1473 9
SphI G_CATG'C
Cuts at: 0 208 1482
Size: 208 1274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 650 1400 1482
Size: 650 750 82
Fragments arranged by size:
750 650 82
TatI w'GTAC_w
Cuts at: 0 11 308 1482
Size: 11 297 1174
Fragments arranged by size:
1174 297 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1462 1482
Size: 1462 20
Tth111I GACn'n_nGTC
Cuts at: 0 844 1320 1482
Size: 844 476 162
Fragments arranged by size:
844 476 162
XbaI T'CTAG_A
Cuts at: 0 1420 1456 1482
Size: 1420 36 26
Fragments arranged by size:
1420 36 26
XmaI C'CCGG_G
Cuts at: 0 38 1482
Size: 38 1444
Enzymes that do cut:
AcuI AfeI ApoI AvaI BanI BanII BbsI BglI
BglII BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I
BsrBI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII
Hin4I MmeI MspA1I NspI PfoI PpuMI PshAI PvuII
SacII SapI SmaI SmlI SphI TaqII TatI TstI
Tth111I XbaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BlpI Bme1580I BmgBI BmtI BsaAI
BsaWI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI StyI SwaI XcmI XhoI XmnI
ZraI