Restriction map for
pFN21AB5869
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BanI BbeI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI BstUI CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Van91I
XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 457 GTATAC GT^[AC][GT]AC
AccIII 1227 TCCGGA T^CCGGA
ApaI 231 GGGCCC GGGCC^C
Asp700I 8 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 889 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 355 CCCGGG C^[CT]CG[GA]G
BbrPI 519 CACGTG CAC^GTG
BbsI 694 GAAGAC GAAGAC(2/6)
BciVI 1224 GTATCC GTATCC(6/5)
BfaI 718 CTAG C^TAG
BglI 59 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 694 GAAGAC GAAGAC(2/6)
BsaAI 519 CACGTG [CT]AC^GT[GA]
BsaI 1369 GAGACC GGTCTC(1/5)
BseAI 1227 TCCGGA T^CCGGA
BseRI 642 GAGGAG GAGGAG(10/8)
BsoBI 355 CCCGGG C^[CT]CG[GA]G
BspEI 1227 TCCGGA T^CCGGA
BspLU11I 993 ACATGT A^CATGT
BspMI 1241 GCAGGT ACCTGC(4/8)
BsrBI 948 CCGCTC CCGCTC(-3/-3)
Bst1107I 457 GTATAC GTA^TAC
BstXI 1014 CCACAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 457 GTATAC GTA^TAC
HaeII 255 AGCGCC [GA]GCGC^[CT]
HgaI 555 GCGTC GACGC(5/10)
HincII 307 GTCAAC GT[CT]^[GA]AC
HindII 307 GTCAAC GT[CT]^[GA]AC
MaeI 718 CTAG C^TAG
MaeII 520 ACGT A^CGT
MroI 1227 TCCGGA T^CCGGA
NcoI 227 CCATGG C^CATGG
NdeI 804 CATATG CA^TATG
PflFI 889 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 519 CACGTG CAC^GTG
PmlI 519 CACGTG CAC^GTG
PshAI 550 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1378 CTGCAG CTGCA^G
ScaI 575 AGTACT AGT^ACT
SexAI 890 ACCAGGT A^CC[AT]GGT
SfcI 1378 CTGCAG C^T[GA][CT]AG
SmaI 355 CCCGGG CCC^GGG
SmlI 1201 CTTGAG C^T[CT][GA]AG
StuI 1205 AGGCCT AGG^CCT
Tsp45I 761 GTGAC ^GT[GC]AC
Tth111I 889 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 717 TCTAGA T^CTAGA
XmaCI 355 CCCGGG C^CCGGG
XmaI 355 CCCGGG C^CCGGG
XmnI 8 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 576 GTAC GT^AC
1123 GTAC
AlwI 434 GATCC GGATC(4/5)
394 GGATC
AocI 239 CCTCAGG CC^T[ACGT]AGG
437 CCTCAGG
AspHI 245 GAGCAC G[AT]GC[AT]^C
1303 GAGCAC
BalI 57 TGGCCA TGG^CCA
980 TGGCCA
BpmI 260 CTCCAG CTGGAG(16/14)
1300 CTGGAG
BsaWI 1001 ACCGGA [AT]^CCGG[AT]
1227 TCCGGA
BsgI 170 GTGCAG GTGCAG(16/14)
961 GTGCAG
BsiHKAI 245 GAGCAC G[AT]GC[AT]^C
1303 GAGCAC
BsmFI 178 GGGAC GGGAC(10/14)
1331 GGGAC
BspHI 23 TCATGA T^CATGA
690 TCATGA
BstAPI 563 GCAACCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
670 GCAGAATGTGC
BstYI 394 GGATCT [GA]^GATC[CT]
433 AGATCC
Bsu36I 239 CCTCAGG CC^T[ACGT]AGG
437 CCTCAGG
EaeI 57 TGGCCA [CT]^GGCC[GA]
980 TGGCCA
EarI 1309 CTCTTC CTCTTC(1/4)
612 GAAGAG
Eco81I 239 CCTCAGG CC^T[ACGT]AGG
437 CCTCAGG
FauI 34 CCCGC CCCGC(4/6)
1374 CCCGC
Ksp632I 1309 CTCTTC CTCTTC(1/4)
612 GAAGAG
MaeIII 761 GTGAC ^GT[ACGT]AC
796 GTTAC
MflI 394 GGATCT [GA]^GATC[CT]
433 AGATCC
MluNI 57 TGGCCA TGG^CCA
980 TGGCCA
MscI 57 TGGCCA TGG^CCA
980 TGGCCA
MseI 404 TTAA T^TAA
1130 TTAA
MspA1I 567 CCGCTG C[AC]G^C[GT]G
1375 CCGCTG
PpuMI 178 GGGACCC [GA]G^G[AT]CC[CT]
506 AGGACCT
RcaI 23 TCATGA T^CATGA
690 TCATGA
RsaI 576 GTAC GT^AC
1123 GTAC
TaqI 210 TCGA T^CGA
781 TCGA
Tru9I 404 TTAA T^TAA
1130 TTAA
Tsp509I 10 AATT ^AATT
923 AATT
TthHB8I 210 TCGA T^CGA
781 TCGA
XhoII 394 GGATCT [GA]^GATC[CT]
433 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AflIII AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI TseI TspRI