(Circular) (Six-base) MAPSORT of: pFN21AB5869.seq Check: 5806 from: 1 to: 6094 pFN21AB5869 6094 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3904 278 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2463 3437 1937 Size: 526 974 4594 Fragments arranged by size: 4594 974 526 Acc65I G'GTAC_C Cuts at: 3415 3415 Size: 6094 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4157 5541 5809 1632 Size: 2525 1384 268 1917 Fragments arranged by size: 2525 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5897 AflIII A'CryG_T Cuts at: 1203 2523 2525 2998 4993 5970 1203 Size: 1320 2 473 1995 977 1327 Fragments arranged by size: 1995 1327 1320 977 473 2 AgeI A'CCGG_T Cuts at: 4925 4925 Size: 6094 AhdI GACnn_n'nnGTC Cuts at: 4823 4823 Size: 6094 AleI CACnn'nnGTG Cuts at: 1166 2418 1166 Size: 1252 4842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6094 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2369 2401 2369 Size: 32 6062 AlwNI CAG_nnn'CTG Cuts at: 3837 5409 3837 Size: 1572 4522 ApaI G_GGCC'C Cuts at: 2240 2240 Size: 6094 ApaLI G'TGCA_C Cuts at: 4151 5307 4151 Size: 1156 4938 ApoI r'AATT_y Cuts at: 1493 3403 3629 3781 5890 1493 Size: 1910 226 152 2109 1697 Fragments arranged by size: 2109 1910 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 2360 3419 1870 Size: 73 417 1059 4545 Fragments arranged by size: 4545 1059 417 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2136 2169 2136 Size: 33 6061 BamHI G'GATC_C Cuts at: 3424 3791 3424 Size: 367 5727 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3415 4870 618 Size: 325 217 627 1628 1455 1842 Fragments arranged by size: 1842 1628 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2090 2207 2240 3413 1391 Size: 699 117 33 1173 4072 Fragments arranged by size: 4072 1173 699 117 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2706 928 Size: 971 807 4316 Fragments arranged by size: 4316 971 807 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4285 4319 1903 Size: 34 2348 34 3678 Fragments arranged by size: 3678 2348 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3240 3986 5196 3240 Size: 746 1210 4138 Fragments arranged by size: 4138 1210 746 BclI T'GATC_A Cuts at: 1548 1548 Size: 6094 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2070 4705 136 Size: 107 122 71 631 605 398 2635 1525 Fragments arranged by size: 2635 1525 631 605 398 122 107 71 BglII A'GATC_T Cuts at: 6089 6089 Size: 6094 BlpI GC'TnA_GC Cuts at: 3511 3511 Size: 6094 Bme1580I G_kGCm'C Cuts at: 1790 2161 2240 3019 4155 5311 1790 Size: 371 79 779 1136 1156 2573 Fragments arranged by size: 2573 1156 1136 779 371 79 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6094 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2144 3116 3825 Size: 416 53 83 399 24 727 972 709 Cuts at: 3825 4783 442 Size: 958 1753 Fragments arranged by size: 1753 972 958 727 709 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6094 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2250 3326 4754 1239 Size: 1011 1076 1428 2579 Fragments arranged by size: 2579 1428 1076 1011 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3227 4219 5084 5382 3227 Size: 992 865 298 3939 Fragments arranged by size: 3939 992 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3368 4757 882 Size: 617 1869 1389 2219 Fragments arranged by size: 2219 1869 1389 617 BsaAI yAC'GTr Cuts at: 493 1204 2526 3885 493 Size: 711 1322 1359 2702 Fragments arranged by size: 2702 1359 1322 711 BsaBI GATnn'nnATC Cuts at: 6088 6088 Size: 6094 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3901 4283 4918 275 Size: 53 83 186 936 2368 382 635 1451 Fragments arranged by size: 2368 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3006 3232 4527 4925 5199 5346 5757 3006 Size: 226 1295 398 274 147 411 3343 Fragments arranged by size: 3343 1295 411 398 274 226 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5869 Fragments arranged by size: 5869 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2662 1186 Size: 1476 4618 BseYI C'CCAG_C Cuts at: 2132 2291 3026 3274 5297 5793 2132 Size: 159 735 248 2023 496 2433 Fragments arranged by size: 2433 2023 735 496 248 159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2196 2987 1163 Size: 573 460 791 4270 Fragments arranged by size: 4270 791 573 460 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3544 4305 5333 1540 Size: 275 186 1543 761 1028 2301 Fragments arranged by size: 2301 1543 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2254 3312 4155 4240 5311 2254 Size: 1058 843 85 1071 3037 Fragments arranged by size: 3037 1071 1058 843 85 BsmI GAATG_Cn' Cuts at: 3605 3698 3605 Size: 93 6001 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2161 2207 2240 2254 3019 3312 Size: 399 300 71 46 33 14 765 293 Cuts at: 3312 3413 4155 4240 5311 1391 Size: 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 765 742 399 300 293 101 85 71 46 33 14 BspEI T'CCGG_A Cuts at: 3232 5757 3232 Size: 2525 3569 BspHI T'CATG_A Cuts at: 2028 2695 3983 5713 2028 Size: 667 1288 1730 2409 Fragments arranged by size: 2409 1730 1288 667 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3237 3449 844 Size: 2393 212 3489 Fragments arranged by size: 3489 2393 212 BsrBI CCG'CTC Cuts at: 1394 1823 2955 3981 1394 Size: 429 1132 1026 3507 Fragments arranged by size: 3507 1132 1026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4589 4763 65 Size: 1183 3341 174 1396 Fragments arranged by size: 3341 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4738 4925 1700 Size: 110 2928 187 2869 Fragments arranged by size: 2928 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6094 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6094 BssSI C'ACGA_G Cuts at: 4154 5166 4154 Size: 1012 5082 BstAPI GCAn_nnn'nTGC Cuts at: 794 2574 2681 794 Size: 1780 107 4207 Fragments arranged by size: 4207 1780 107 BstBI TT'CG_AA Cuts at: 4898 4898 Size: 6094 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6094 BstXI CCAn_nnnn'nTGG Cuts at: 3026 3026 Size: 6094 BstYI r'GATC_y Cuts at: 1972 2399 2438 3424 3791 4176 4193 5645 5731 Size: 427 39 986 367 385 17 1452 86 Cuts at: 5731 5743 6089 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 986 427 385 367 346 86 39 17 12 BstZ17I GTA'TAC Cuts at: 2464 2464 Size: 6094 Bsu36I CC'TnA_GG Cuts at: 2245 2443 2245 Size: 198 5896 BtgI C'CryG_G Cuts at: 513 1065 2162 2232 2988 513 Size: 552 1097 70 756 3619 Fragments arranged by size: 3619 1097 756 552 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5355 Fragments arranged by size: 5355 683 56 BtsI GCAGTG_nn' Cuts at: 2184 3019 3610 4404 4424 2184 Size: 835 591 794 20 3854 Fragments arranged by size: 3854 835 794 591 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6094 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6059 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5101 809 Size: 507 3785 1802 Fragments arranged by size: 3785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2985 3541 Size: 54 1425 50 275 18 232 923 556 Cuts at: 3541 4430 6059 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 923 889 556 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3541 1537 Size: 275 1729 4090 Fragments arranged by size: 4090 1729 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2612 3320 4024 1353 Size: 1259 708 704 3423 Fragments arranged by size: 3423 1259 708 704 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4681 5055 5201 387 Size: 1064 138 3092 374 146 1280 Fragments arranged by size: 3092 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 3326 4157 4754 5541 5809 1239 Size: 393 618 1076 831 597 787 268 1524 Fragments arranged by size: 1524 1076 831 787 618 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2184 2512 3151 2184 Size: 328 639 5127 Fragments arranged by size: 5127 639 328 EcoRI G'AATT_C Cuts at: 3403 3781 3403 Size: 378 5716 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2750 2750 Size: 6094 FspI TGC'GCA Cuts at: 4600 4600 Size: 6094 HaeII r_GCGC'y Cuts at: 1678 2264 5241 1678 Size: 586 2977 2531 Fragments arranged by size: 2977 2531 586 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2426 2458 4735 4767 4809 Size: 32 135 32 854 32 2277 32 42 Cuts at: 4809 4841 1373 Size: 32 2626 Fragments arranged by size: 2626 2277 854 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2314 3438 3684 669 Size: 988 281 376 1124 246 3079 Fragments arranged by size: 3079 1124 988 376 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6094 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KpnI G_GTAC'C Cuts at: 3419 3419 Size: 6094 MfeI C'AATT_G Cuts at: 3693 3693 Size: 6094 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3081 3183 3351 5208 5392 1305 Size: 1776 102 168 1857 184 2007 Fragments arranged by size: 2007 1857 1776 184 168 102 MscI TGG'CCA Cuts at: 10 64 1489 1832 2064 2987 10 Size: 54 1425 343 232 923 3117 Fragments arranged by size: 3117 1425 923 343 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 2418 2612 2708 2774 3089 3365 4052 Size: 648 1252 194 96 66 315 276 687 Cuts at: 4052 4411 4570 518 Size: 359 159 2042 Fragments arranged by size: 2042 1252 687 648 359 315 276 194 159 96 66 MspA1I CmG'CkG Cuts at: 1606 2574 3382 3510 4187 5335 5580 1606 Size: 968 808 128 677 1148 245 2120 Fragments arranged by size: 2120 1148 968 808 677 245 128 NcoI C'CATG_G Cuts at: 513 1065 2232 513 Size: 552 1167 4375 Fragments arranged by size: 4375 1167 552 NdeI CA'TA_TG Cuts at: 387 2810 387 Size: 2423 3671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6094 NotI GC'GGCC_GC Cuts at: 3541 3541 Size: 6094 NspI r_CATG'y Cuts at: 1132 1229 2707 3002 3088 3452 4997 1132 Size: 97 1478 295 86 364 1545 2229 Fragments arranged by size: 2229 1545 1478 364 295 97 86 PasI CC'CwG_GG Cuts at: 1348 2200 1348 Size: 852 5242 PciI A'CATG_T Cuts at: 2998 4993 2998 Size: 1995 4099 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6094 PfoI T'CCnGG_A Cuts at: 2266 2266 Size: 6094 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PmlI CAC'GTG Cuts at: 2526 2526 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2184 2512 2184 Size: 328 5766 PshAI GACnn'nnGTC Cuts at: 1613 2559 1613 Size: 946 5148 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PspOMI G'GGCC_C Cuts at: 2236 2236 Size: 6094 PstI C_TGCA'G Cuts at: 830 3387 3446 830 Size: 2557 59 3478 Fragments arranged by size: 3478 2557 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SanDI GG'GwC_CC Cuts at: 2184 2184 Size: 6094 SbfI CC_TGCA'GG Cuts at: 3446 3446 Size: 6094 ScaI AGT'ACT Cuts at: 2582 4342 6054 2582 Size: 1760 1712 2622 Fragments arranged by size: 2622 1760 1712 SexAI A'CCwGG_T Cuts at: 2895 2895 Size: 6094 SfcI C'TryA_G Cuts at: 826 1045 3383 3442 4577 5258 5449 826 Size: 219 2338 59 1135 681 191 1471 Fragments arranged by size: 2338 1471 1135 681 219 191 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6094 SmaI CCC'GGG Cuts at: 1872 2362 3421 1872 Size: 490 1059 4545 Fragments arranged by size: 4545 1059 490 SmlI C'TyrA_G Cuts at: 820 1017 1943 3206 4198 5099 5361 820 Size: 197 926 1263 992 901 262 1553 Fragments arranged by size: 1553 1263 992 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6094 SphI G_CATG'C Cuts at: 1132 3452 1132 Size: 2320 3774 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 3212 1517 Size: 1695 4399 StyI C'CwwG_G Cuts at: 513 1065 2232 2886 3147 3533 513 Size: 552 1167 654 261 386 3074 Fragments arranged by size: 3074 1167 654 552 386 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2305 4121 4138 4291 4476 4921 325 Size: 1255 725 1816 17 153 185 445 1498 Fragments arranged by size: 1816 1498 1255 725 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2580 3127 4340 Size: 274 80 33 51 1443 603 547 1213 Cuts at: 4340 6052 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1213 603 547 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2158 2190 6054 6086 2158 Size: 32 3864 32 2166 Fragments arranged by size: 3864 2166 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2897 1316 Size: 1581 4513 XbaI T'CTAG_A Cuts at: 2722 3430 2722 Size: 708 5386 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6094 XmaI C'CCGG_G Cuts at: 1870 2360 3419 1870 Size: 490 1059 4545 Fragments arranged by size: 4545 1059 490 XmnI GAAnn'nnTTC Cuts at: 2017 4223 2017 Size: 2206 3888 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3902 276 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI