Restriction map for
pFN21AB5869
(Linear) (Six-base) MAPSORT of: KIBB5869.seq Check: 2081 from: 1 to: 1389
KIBB5869.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 0 458 1389
Size: 458 931
AflIII A'CryG_T
Cuts at: 0 518 520 993 1389
Size: 518 2 473 396
Fragments arranged by size:
518 473 396 2
AleI CACnn'nnGTG
Cuts at: 0 413 1389
Size: 413 976
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 364 396 1389
Size: 364 32 993
Fragments arranged by size:
993 364 32
ApaI G_GGCC'C
Cuts at: 0 235 1389
Size: 235 1154
AvaI C'yCGr_G
Cuts at: 0 355 1389
Size: 355 1034
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 131 164 1389
Size: 131 33 1225
Fragments arranged by size:
1225 131 33
BanII G_rGCy'C
Cuts at: 0 85 202 235 1389
Size: 85 117 33 1154
Fragments arranged by size:
1154 117 85 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 701 1389
Size: 701 688
BciVI GTATCCnnnnn_n'
Cuts at: 0 1235 1389
Size: 1235 154
BglI GCCn_nnn'nGGC
Cuts at: 0 65 1389
Size: 65 1324
Bme1580I G_kGCm'C
Cuts at: 0 156 235 1014 1389
Size: 156 79 779 375
Fragments arranged by size:
779 375 156 79
BmrI ACTGGGnnnn_n'
Cuts at: 0 139 1111 1389
Size: 139 972 278
Fragments arranged by size:
972 278 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1321 1389
Size: 245 1076 68
Fragments arranged by size:
1076 245 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1389
Size: 1222 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 1363 1389
Size: 1363 26
BsaAI yAC'GTr
Cuts at: 0 521 1389
Size: 521 868
BsaWI w'CCGG_w
Cuts at: 0 1001 1227 1389
Size: 1001 226 162
Fragments arranged by size:
1001 226 162
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 657 1389
Size: 657 732
BseYI C'CCAG_C
Cuts at: 0 127 286 1021 1269 1389
Size: 127 159 735 248 120
Fragments arranged by size:
735 248 159 127 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 982 1389
Size: 191 791 407
Fragments arranged by size:
791 407 191
BsiHKAI G_wGCw'C
Cuts at: 0 249 1307 1389
Size: 249 1058 82
Fragments arranged by size:
1058 249 82
Bsp1286I G_dGCh'C
Cuts at: 0 85 156 202 235 249 1014 1307 1389
Size: 85 71 46 33 14 765 293 82
Fragments arranged by size:
765 293 85 82 71 46 33 14
BspEI T'CCGG_A
Cuts at: 0 1227 1389
Size: 1227 162
BspHI T'CATG_A
Cuts at: 0 23 690 1389
Size: 23 667 699
Fragments arranged by size:
699 667 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1232 1389
Size: 1232 157
BsrBI CCG'CTC
Cuts at: 0 950 1389
Size: 950 439
BstAPI GCAn_nnn'nTGC
Cuts at: 0 569 676 1389
Size: 569 107 713
Fragments arranged by size:
713 569 107
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1021 1389
Size: 1021 368
BstYI r'GATC_y
Cuts at: 0 394 433 1389
Size: 394 39 956
Fragments arranged by size:
956 394 39
BstZ17I GTA'TAC
Cuts at: 0 459 1389
Size: 459 930
Bsu36I CC'TnA_GG
Cuts at: 0 240 438 1389
Size: 240 198 951
Fragments arranged by size:
951 240 198
BtgI C'CryG_G
Cuts at: 0 157 227 983 1389
Size: 157 70 756 406
Fragments arranged by size:
756 406 157 70
BtsI GCAGTG_nn'
Cuts at: 0 179 1014 1389
Size: 179 835 375
Fragments arranged by size:
835 375 179
EaeI y'GGCC_r
Cuts at: 0 57 980 1389
Size: 57 923 409
Fragments arranged by size:
923 409 57
EarI CTCTTCn'nnn_
Cuts at: 0 607 1315 1389
Size: 607 708 74
Fragments arranged by size:
708 607 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1321 1389
Size: 245 1076 68
Fragments arranged by size:
1076 245 68
EcoO109I rG'GnC_Cy
Cuts at: 0 179 507 1146 1389
Size: 179 328 639 243
Fragments arranged by size:
639 328 243 179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 745 1389
Size: 745 644
HaeII r_GCGC'y
Cuts at: 0 259 1389
Size: 259 1130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 1389
Size: 421 32 936
Fragments arranged by size:
936 421 32
HincII GTy'rAC
Cuts at: 0 309 1389
Size: 309 1080
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1178 1346 1389
Size: 1076 102 168 43
Fragments arranged by size:
1076 168 102 43
MscI TGG'CCA
Cuts at: 0 59 982 1389
Size: 59 923 407
Fragments arranged by size:
923 407 59
MslI CAynn'nnrTG
Cuts at: 0 413 607 703 769 1084 1360 1389
Size: 413 194 96 66 315 276 29
Fragments arranged by size:
413 315 276 194 96 66 29
MspA1I CmG'CkG
Cuts at: 0 569 1377 1389
Size: 569 808 12
Fragments arranged by size:
808 569 12
NcoI C'CATG_G
Cuts at: 0 227 1389
Size: 227 1162
NdeI CA'TA_TG
Cuts at: 0 805 1389
Size: 805 584
NspI r_CATG'y
Cuts at: 0 702 997 1083 1389
Size: 702 295 86 306
Fragments arranged by size:
702 306 295 86
PasI CC'CwG_GG
Cuts at: 0 195 1389
Size: 195 1194
PciI A'CATG_T
Cuts at: 0 993 1389
Size: 993 396
PfoI T'CCnGG_A
Cuts at: 0 261 1389
Size: 261 1128
PmlI CAC'GTG
Cuts at: 0 521 1389
Size: 521 868
PpuMI rG'GwC_Cy
Cuts at: 0 179 507 1389
Size: 179 328 882
Fragments arranged by size:
882 328 179
PshAI GACnn'nnGTC
Cuts at: 0 554 1389
Size: 554 835
PspOMI G'GGCC_C
Cuts at: 0 231 1389
Size: 231 1158
PstI C_TGCA'G
Cuts at: 0 1382 1389
Size: 1382 7
SanDI GG'GwC_CC
Cuts at: 0 179 1389
Size: 179 1210
ScaI AGT'ACT
Cuts at: 0 577 1389
Size: 577 812
SexAI A'CCwGG_T
Cuts at: 0 890 1389
Size: 890 499
SfcI C'TryA_G
Cuts at: 0 1378 1389
Size: 1378 11
SmaI CCC'GGG
Cuts at: 0 357 1389
Size: 357 1032
SmlI C'TyrA_G
Cuts at: 0 1201 1389
Size: 1201 188
StuI AGG'CCT
Cuts at: 0 1207 1389
Size: 1207 182
StyI C'CwwG_G
Cuts at: 0 227 881 1142 1389
Size: 227 654 261 247
Fragments arranged by size:
654 261 247 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 300 1389
Size: 300 1089
TatI w'GTAC_w
Cuts at: 0 575 1122 1389
Size: 575 547 267
Fragments arranged by size:
575 547 267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 153 185 1389
Size: 153 32 1204
Fragments arranged by size:
1204 153 32
Tth111I GACn'n_nGTC
Cuts at: 0 892 1389
Size: 892 497
XbaI T'CTAG_A
Cuts at: 0 717 1389
Size: 717 672
XmaI C'CCGG_G
Cuts at: 0 355 1389
Size: 355 1034
XmnI GAAnn'nnTTC
Cuts at: 0 12 1389
Size: 12 1377
Enzymes that do cut:
AccI AflIII AleI AloI ApaI AvaI BaeI BanII
BbsI BciVI BglI Bme1580I BmrI BpmI BpuEI BsaI
BsaAI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BstAPI BstXI BstYI BstZ17I Bsu36I
BtgI BtsI EaeI EarI Eco57MI EcoO109I FalI HaeII
Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI
NspI PasI PciI PfoI PmlI PpuMI PshAI PspOMI
PstI SanDI ScaI SexAI SfcI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BanI BbeI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaBI BsaHI
BsaXI BsiEI BsiWI BsmI BsmBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PpiI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XcmI XhoI ZraI