(Circular) (Six-base) MAPSORT of: pFN21AB5706.seq Check: 2111 from: 1 to: 6856 pFN21AB5706 6856 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4666 278 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3505 4199 1937 Size: 1568 694 4594 Fragments arranged by size: 4594 1568 694 Acc65I G'GTAC_C Cuts at: 4177 4177 Size: 6856 AclI AA'CG_TT Cuts at: 1563 4983 5356 1563 Size: 3420 373 3063 Fragments arranged by size: 3420 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4919 6303 6571 1632 Size: 3287 1384 268 1917 Fragments arranged by size: 3287 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6659 AflIII A'CryG_T Cuts at: 1203 2656 5755 6732 1203 Size: 1453 3099 977 1327 Fragments arranged by size: 3099 1453 1327 977 AgeI A'CCGG_T Cuts at: 5687 5687 Size: 6856 AhdI GACnn_n'nnGTC Cuts at: 5585 5585 Size: 6856 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6856 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6856 AlwNI CAG_nnn'CTG Cuts at: 2205 2854 4599 6171 2205 Size: 649 1745 1572 2890 Fragments arranged by size: 2890 1745 1572 649 ApaI G_GGCC'C Cuts at: 3463 3994 3463 Size: 531 6325 ApaLI G'TGCA_C Cuts at: 4913 6069 4913 Size: 1156 5700 ApoI r'AATT_y Cuts at: 1493 3152 3927 4165 4391 4543 6652 1493 Size: 1659 775 238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1659 775 238 226 152 AseI AT'TA_AT Cuts at: 160 1032 5410 160 Size: 872 4378 1606 Fragments arranged by size: 4378 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 AvaI C'yCGr_G Cuts at: 1870 1943 3393 4181 1870 Size: 73 1450 788 4545 Fragments arranged by size: 4545 1450 788 73 BamHI G'GATC_C Cuts at: 4186 4553 4186 Size: 367 6489 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2713 4177 5632 618 Size: 325 217 627 926 1464 1455 1842 Fragments arranged by size: 1842 1464 1455 926 627 325 217 BanII G_rGCy'C Cuts at: 1391 3463 3994 4175 1391 Size: 2072 531 181 4072 Fragments arranged by size: 4072 2072 531 181 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2165 928 Size: 971 266 5619 Fragments arranged by size: 5619 971 266 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5047 5081 1903 Size: 34 3110 34 3678 Fragments arranged by size: 3678 3110 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4748 5958 4748 Size: 1210 5646 BclI T'GATC_A Cuts at: 1548 2544 1548 Size: 996 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5467 136 Size: 107 122 71 631 605 3795 1525 Fragments arranged by size: 3795 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6851 6851 Size: 6856 BlpI GC'TnA_GC Cuts at: 3660 4273 3660 Size: 613 6243 Bme1580I G_kGCm'C Cuts at: 1790 3463 3994 4917 6073 1790 Size: 1673 531 923 1156 2573 Fragments arranged by size: 2573 1673 1156 923 531 BmgBI CAC'GTC Cuts at: 1318 2657 1318 Size: 1339 5517 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2176 2184 2893 Size: 416 53 83 399 24 759 8 709 Cuts at: 2893 3680 3830 4587 5545 442 Size: 787 150 757 958 1753 Fragments arranged by size: 1753 958 787 759 757 709 416 399 150 83 53 24 8 BmtI G_CTAG'C Cuts at: 1056 3668 4004 1056 Size: 2612 336 3908 Fragments arranged by size: 3908 2612 336 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2808 2808 Size: 6856 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2584 3468 3786 5516 1239 Size: 1345 884 318 1730 2579 Fragments arranged by size: 2579 1730 1345 884 318 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2358 2718 2927 4981 5846 6144 2358 Size: 360 209 2054 865 298 3070 Fragments arranged by size: 3070 2054 865 360 298 209 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5519 882 Size: 617 4020 2219 Fragments arranged by size: 4020 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4647 493 Size: 711 3443 2702 Fragments arranged by size: 3443 2702 711 BsaBI GATnn'nnATC Cuts at: 3603 6850 3603 Size: 3247 3609 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4663 5045 5680 275 Size: 53 83 186 936 3130 382 635 1451 Fragments arranged by size: 3130 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3679 5289 5687 5961 6108 6519 3679 Size: 1610 398 274 147 411 4016 Fragments arranged by size: 4016 1610 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4089 4119 1178 Size: 30 165 30 2686 30 3915 Fragments arranged by size: 3915 2686 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2709 2721 3867 1186 Size: 1523 12 1146 4175 Fragments arranged by size: 4175 1523 1146 12 BseYI C'CCAG_C Cuts at: 2863 3307 6059 6555 2863 Size: 444 2752 496 3164 Fragments arranged by size: 3164 2752 496 444 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6283 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4306 5067 6095 1540 Size: 275 186 2305 761 1028 2301 Fragments arranged by size: 2305 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4135 4917 5002 6073 4135 Size: 782 85 1071 4918 Fragments arranged by size: 4918 1071 782 85 BsmI GAATG_Cn' Cuts at: 2112 2653 3536 4367 4460 2112 Size: 541 883 831 93 4508 Fragments arranged by size: 4508 883 831 541 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2007 2007 Size: 6856 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3463 3994 4135 4175 4917 5002 6073 Size: 399 1673 531 141 40 742 85 1071 Cuts at: 6073 1391 Size: 2174 Fragments arranged by size: 2174 1673 1071 742 531 399 141 85 40 BspEI T'CCGG_A Cuts at: 3679 6519 3679 Size: 2840 4016 BspHI T'CATG_A Cuts at: 4117 4745 6475 4117 Size: 628 1730 4498 Fragments arranged by size: 4498 1730 628 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4211 844 Size: 3367 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4743 1394 Size: 429 2920 3507 Fragments arranged by size: 3507 2920 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5351 5525 65 Size: 1183 4103 174 1396 Fragments arranged by size: 4103 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5500 5687 1700 Size: 110 3690 187 2869 Fragments arranged by size: 3690 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2214 3904 96 Size: 2118 1690 3048 Fragments arranged by size: 3048 2118 1690 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6856 BssSI C'ACGA_G Cuts at: 4916 5928 4916 Size: 1012 5844 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6856 BstBI TT'CG_AA Cuts at: 5660 5660 Size: 6856 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6856 BstYI r'GATC_y Cuts at: 1972 2474 4186 4553 4938 4955 6407 6493 6505 Size: 502 1712 367 385 17 1452 86 12 Cuts at: 6505 6851 1972 Size: 346 1977 Fragments arranged by size: 1977 1712 1452 502 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3506 3506 Size: 6856 BtgI C'CryG_G Cuts at: 513 1065 2022 4023 513 Size: 552 957 2001 3346 Fragments arranged by size: 3346 2001 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6117 Fragments arranged by size: 6117 683 56 BtsI GCAGTG_nn' Cuts at: 4372 5166 5186 4372 Size: 794 20 6042 Fragments arranged by size: 6042 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6856 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6821 DraI TTT'AAA Cuts at: 3199 4160 3199 Size: 961 5895 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5863 809 Size: 507 4547 1802 Fragments arranged by size: 4547 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4303 5192 6821 Size: 54 1425 50 275 18 2473 889 1629 Cuts at: 6821 8 Size: 43 Fragments arranged by size: 2473 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4303 1537 Size: 275 2491 4090 Fragments arranged by size: 4090 2491 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2506 2580 2799 3048 3540 4786 1353 Size: 723 430 74 219 249 492 1246 3423 Fragments arranged by size: 3423 1246 723 492 430 249 219 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5443 5817 5963 387 Size: 1064 138 3854 374 146 1280 Fragments arranged by size: 3854 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2584 3468 3786 4919 5516 6303 6571 Size: 393 952 884 318 1133 597 787 268 Cuts at: 6571 1239 Size: 1524 Fragments arranged by size: 1524 1133 952 884 787 597 393 318 268 EcoNI CCTnn'n_nnAGG Cuts at: 2282 3377 2282 Size: 1095 5761 EcoO109I rG'GnC_Cy Cuts at: 3896 3990 3991 3896 Size: 94 1 6761 Fragments arranged by size: 6761 94 1 EcoRI G'AATT_C Cuts at: 4165 4543 4165 Size: 378 6478 FspI TGC'GCA Cuts at: 5362 5362 Size: 6856 HaeII r_GCGC'y Cuts at: 1678 6003 1678 Size: 4325 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2611 2643 4089 4121 5497 Size: 32 135 32 1039 32 1446 32 1376 Cuts at: 5497 5529 5571 5603 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1446 1376 1039 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4200 4446 669 Size: 988 281 2262 246 3079 Fragments arranged by size: 3079 2262 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6856 HpaI GTT'AAC Cuts at: 4446 4446 Size: 6856 KpnI G_GTAC'C Cuts at: 4181 4181 Size: 6856 MfeI C'AATT_G Cuts at: 3108 4455 3108 Size: 1347 5509 MluI A'CGCG_T Cuts at: 6732 6732 Size: 6856 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5970 6154 1305 Size: 4665 184 2007 Fragments arranged by size: 4665 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5034 Fragments arranged by size: 5034 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3237 3375 4814 5173 5332 518 Size: 648 2071 138 1439 359 159 2042 Fragments arranged by size: 2071 2042 1439 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2036 2504 3851 4272 4949 6097 6342 1606 Size: 430 468 1347 421 677 1148 245 2120 Fragments arranged by size: 2120 1347 1148 677 468 430 421 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6304 NdeI CA'TA_TG Cuts at: 387 2092 2323 2380 3040 387 Size: 1705 231 57 660 4203 Fragments arranged by size: 4203 1705 660 231 57 NheI G'CTAG_C Cuts at: 1052 3664 4000 1052 Size: 2612 336 3908 Fragments arranged by size: 3908 2612 336 NotI GC'GGCC_GC Cuts at: 4303 4303 Size: 6856 NspI r_CATG'y Cuts at: 1132 1229 4214 5759 1132 Size: 97 2985 1545 2229 Fragments arranged by size: 2985 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6856 PciI A'CATG_T Cuts at: 5755 5755 Size: 6856 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6856 PmeI GTTT'AAAC Cuts at: 4160 4160 Size: 6856 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4925 4957 6438 6470 4925 Size: 32 1481 32 5311 Fragments arranged by size: 5311 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3896 3896 Size: 6856 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6856 PsiI TTA'TAA Cuts at: 2819 3642 4426 2819 Size: 823 784 5249 Fragments arranged by size: 5249 823 784 PspOMI G'GGCC_C Cuts at: 3459 3990 3459 Size: 531 6325 PstI C_TGCA'G Cuts at: 830 3250 3850 4208 830 Size: 2420 600 358 3478 Fragments arranged by size: 3478 2420 600 358 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6856 PvuII CAG'CTG Cuts at: 2036 2504 3851 2036 Size: 468 1347 5041 Fragments arranged by size: 5041 1347 468 SalI G'TCGA_C Cuts at: 1936 4198 1936 Size: 2262 4594 SbfI CC_TGCA'GG Cuts at: 4208 4208 Size: 6856 ScaI AGT'ACT Cuts at: 4041 5104 6816 4041 Size: 1063 1712 4081 Fragments arranged by size: 4081 1712 1063 SexAI A'CCwGG_T Cuts at: 3820 3820 Size: 6856 SfcI C'TryA_G Cuts at: 826 1045 2142 2469 3246 3268 3702 3846 4005 Size: 219 1097 327 777 22 434 144 159 Cuts at: 4005 4204 5339 6020 6211 826 Size: 199 1135 681 191 1471 Fragments arranged by size: 1471 1135 1097 777 681 434 327 219 199 191 159 144 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6856 SmaI CCC'GGG Cuts at: 1872 4183 1872 Size: 2311 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2373 2733 2906 4960 5861 6123 Size: 197 926 430 360 173 2054 901 262 Cuts at: 6123 820 Size: 1553 Fragments arranged by size: 2054 1553 926 901 430 360 262 197 173 SnaBI TAC'GTA Cuts at: 493 4647 493 Size: 4154 2702 SpeI A'CTAG_T Cuts at: 152 3574 152 Size: 3422 3434 SphI G_CATG'C Cuts at: 1132 4214 1132 Size: 3082 3774 SspI AAT'ATT Cuts at: 5 52 2599 3263 3487 4780 5 Size: 47 2547 664 224 1293 2081 Fragments arranged by size: 2547 2081 1293 664 224 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6856 StyI C'CwwG_G Cuts at: 513 1065 2337 3129 4047 4295 513 Size: 552 1272 792 918 248 3074 Fragments arranged by size: 3074 1272 918 792 552 248 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2422 4883 4900 5053 5238 5683 325 Size: 1255 842 2461 17 153 185 445 1498 Fragments arranged by size: 2461 1498 1255 842 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 3904 4039 Size: 274 80 33 51 1443 237 1690 135 Cuts at: 4039 5102 6814 96 Size: 1063 1712 138 Fragments arranged by size: 1712 1690 1443 1063 274 237 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6816 6848 6816 Size: 32 6824 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6856 XbaI T'CTAG_A Cuts at: 4192 4192 Size: 6856 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6856 XmaI C'CCGG_G Cuts at: 1870 4181 1870 Size: 2311 4545 XmnI GAAnn'nnTTC Cuts at: 3078 4985 3078 Size: 1907 4949 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4664 276 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI