(Circular) (Six-base) MAPSORT of: pFN21AB9981.seq Check: 8295 from: 1 to: 6388 pFN21AB9981 6388 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2602 2602 Size: 6388 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2021 2445 3731 1937 Size: 84 424 1286 4594 Fragments arranged by size: 4594 1286 424 84 Acc65I G'GTAC_C Cuts at: 3709 3709 Size: 6388 AclI AA'CG_TT Cuts at: 1563 4515 4888 1563 Size: 2952 373 3063 Fragments arranged by size: 3063 2952 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3182 4451 5835 6103 1632 Size: 1550 1269 1384 268 1917 Fragments arranged by size: 1917 1550 1384 1269 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6191 AflIII A'CryG_T Cuts at: 1203 5287 6264 1203 Size: 4084 977 1327 Fragments arranged by size: 4084 1327 977 AgeI A'CCGG_T Cuts at: 5219 5219 Size: 6388 AhdI GACnn_n'nnGTC Cuts at: 5117 5117 Size: 6388 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6388 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6388 AlwNI CAG_nnn'CTG Cuts at: 2074 2854 3310 3494 3652 4131 5703 2074 Size: 780 456 184 158 479 1572 2759 Fragments arranged by size: 2759 1572 780 479 456 184 158 ApaI G_GGCC'C Cuts at: 2646 2646 Size: 6388 ApaLI G'TGCA_C Cuts at: 4445 5601 4445 Size: 1156 5232 ApoI r'AATT_y Cuts at: 1493 3697 3923 4075 6184 1493 Size: 2204 226 152 2109 1697 Fragments arranged by size: 2204 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4942 160 Size: 872 3910 1606 Fragments arranged by size: 3910 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2526 2711 3230 3713 1870 Size: 73 91 492 185 519 483 4545 Fragments arranged by size: 4545 519 492 483 185 91 73 BamHI G'GATC_C Cuts at: 3067 3217 3718 4085 3067 Size: 150 501 367 5370 Fragments arranged by size: 5370 501 367 150 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2142 2311 2473 3247 3709 Size: 325 217 627 355 169 162 774 462 Cuts at: 3709 5164 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 774 627 462 355 325 217 169 162 BanII G_rGCy'C Cuts at: 1391 2646 2808 3707 1391 Size: 1255 162 899 4072 Fragments arranged by size: 4072 1255 899 162 BbeI G_GCGC'C Cuts at: 3251 3251 Size: 6388 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5417 BbvCI CC'TCA_GC Cuts at: 2896 2896 Size: 6388 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 1903 Size: 34 2642 34 3678 Fragments arranged by size: 3678 2642 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4280 5490 4280 Size: 1210 5178 BclI T'GATC_A Cuts at: 1548 1548 Size: 6388 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2169 2991 3356 Size: 107 122 71 631 605 497 822 365 Cuts at: 3356 4999 136 Size: 1643 1525 Fragments arranged by size: 1643 1525 822 631 605 497 365 122 107 71 BglII A'GATC_T Cuts at: 2611 3298 6383 2611 Size: 687 3085 2616 Fragments arranged by size: 3085 2616 687 BlpI GC'TnA_GC Cuts at: 3805 3805 Size: 6388 Bme1580I G_kGCm'C Cuts at: 1790 2041 2181 2457 2478 2646 2747 2970 3420 Size: 251 140 276 21 168 101 223 450 Cuts at: 3420 4449 5605 1790 Size: 1029 1156 2573 Fragments arranged by size: 2573 1156 1029 450 276 251 223 168 140 101 21 BmgBI CAC'GTC Cuts at: 1318 2019 1318 Size: 701 5687 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2957 3545 4119 Size: 416 53 83 399 24 1540 588 574 Cuts at: 4119 5077 442 Size: 958 1753 Fragments arranged by size: 1753 1540 958 588 574 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2334 2464 2568 2636 2784 2832 3669 5048 Size: 1095 130 104 68 148 48 837 1379 Cuts at: 5048 1239 Size: 2579 Fragments arranged by size: 2579 1379 1095 837 148 130 104 68 48 Bpu10I CC'TnA_GC Cuts at: 2896 3180 2896 Size: 284 6104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4513 5378 5676 4513 Size: 865 298 5225 Fragments arranged by size: 5225 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5051 882 Size: 617 3552 2219 Fragments arranged by size: 3552 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4179 493 Size: 711 2975 2702 Fragments arranged by size: 2975 2702 711 BsaBI GATnn'nnATC Cuts at: 6382 6382 Size: 6388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3248 4195 4577 5212 Size: 53 83 186 936 1715 947 382 635 Cuts at: 5212 275 Size: 1451 Fragments arranged by size: 1715 1451 947 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3293 4821 5219 5493 5640 6051 3293 Size: 1528 398 274 147 411 3630 Fragments arranged by size: 3630 1528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3248 3278 1178 Size: 30 165 30 1845 30 4288 Fragments arranged by size: 4288 1845 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3155 1186 Size: 1969 4419 BseYI C'CCAG_C Cuts at: 2025 2212 2225 2245 2250 2320 2412 2776 2847 Size: 187 13 20 5 70 92 364 71 Cuts at: 2847 2951 3097 3323 5591 6087 2025 Size: 104 146 226 2268 496 2326 Fragments arranged by size: 2326 2268 496 364 226 187 146 104 92 71 70 20 13 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3193 3641 1163 Size: 573 1457 448 3910 Fragments arranged by size: 3910 1457 573 448 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3838 4599 5627 1540 Size: 275 186 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3511 4449 4534 5605 3511 Size: 938 85 1071 4294 Fragments arranged by size: 4294 1071 938 85 BsmI GAATG_Cn' Cuts at: 3126 3899 3992 3126 Size: 773 93 5522 Fragments arranged by size: 5522 773 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2181 2457 2478 2646 2747 2808 Size: 399 251 140 276 21 168 101 61 Cuts at: 2808 2970 3420 3511 3707 4449 4534 5605 1391 Size: 162 450 91 196 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 399 276 251 196 168 162 140 101 91 85 61 21 BspEI T'CCGG_A Cuts at: 6051 6051 Size: 6388 BspHI T'CATG_A Cuts at: 4277 6007 4277 Size: 1730 4658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2602 3515 3743 844 Size: 1758 913 228 3489 Fragments arranged by size: 3489 1758 913 228 BsrBI CCG'CTC Cuts at: 1394 1823 4275 1394 Size: 429 2452 3507 Fragments arranged by size: 3507 2452 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2010 2293 3335 3545 4883 5057 65 Size: 1183 762 283 1042 210 1338 174 1396 Fragments arranged by size: 1396 1338 1183 1042 762 283 210 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3006 3244 3480 5032 5219 1700 Size: 110 1196 238 236 1552 187 2869 Fragments arranged by size: 2869 1552 1196 238 236 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6388 BssSI C'ACGA_G Cuts at: 4448 5460 4448 Size: 1012 5376 BstAPI GCAn_nnn'nTGC Cuts at: 794 3494 3652 794 Size: 2700 158 3530 Fragments arranged by size: 3530 2700 158 BstBI TT'CG_AA Cuts at: 5192 5192 Size: 6388 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6388 BstXI CCAn_nnnn'nTGG Cuts at: 3562 3562 Size: 6388 BstYI r'GATC_y Cuts at: 1972 2611 2757 3067 3185 3217 3298 3718 4085 Size: 639 146 310 118 32 81 420 367 Cuts at: 4085 4470 4487 5939 6025 6037 6383 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 639 420 385 367 346 310 146 118 86 81 32 17 12 Bsu36I CC'TnA_GG Cuts at: 2341 2943 2341 Size: 602 5786 BtgI C'CryG_G Cuts at: 513 1065 2004 3363 513 Size: 552 939 1359 3538 Fragments arranged by size: 3538 1359 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3434 487 Size: 683 56 2208 3441 Fragments arranged by size: 3441 2208 683 56 BtsI GCAGTG_nn' Cuts at: 3629 3904 4698 4718 3629 Size: 275 794 20 5299 Fragments arranged by size: 5299 794 275 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6388 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 3692 3692 Size: 6388 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5395 809 Size: 507 4079 1802 Fragments arranged by size: 4079 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2161 2856 Size: 54 1425 50 275 18 177 154 695 Cuts at: 2856 3135 3835 4724 6353 8 Size: 279 700 889 1629 43 Fragments arranged by size: 1629 1425 889 700 695 279 275 177 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3835 1537 Size: 275 2023 4090 Fragments arranged by size: 4090 2023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4318 1353 Size: 2965 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2072 4975 5349 5495 387 Size: 1064 138 483 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 483 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 2464 2568 2636 2784 2832 3182 Size: 393 702 130 104 68 148 48 350 Cuts at: 3182 3669 4451 5048 5835 6103 1239 Size: 487 782 597 787 268 1524 Fragments arranged by size: 1524 787 782 702 597 487 393 350 268 148 130 104 68 48 EcoNI CCTnn'n_nnAGG Cuts at: 2979 2979 Size: 6388 EcoO109I rG'GnC_Cy Cuts at: 2104 2345 2409 2642 2643 2883 2947 3050 3192 Size: 241 64 233 1 240 64 103 142 Cuts at: 3192 3259 3649 2104 Size: 67 390 4843 Fragments arranged by size: 4843 390 241 240 233 142 103 67 64 64 1 EcoRI G'AATT_C Cuts at: 3697 4075 3697 Size: 378 6010 FspI TGC'GCA Cuts at: 4894 4894 Size: 6388 HaeII r_GCGC'y Cuts at: 1678 2124 3251 5535 1678 Size: 446 1127 2284 2531 Fragments arranged by size: 2531 2284 1127 446 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2079 2111 2894 2926 3248 Size: 32 135 32 507 32 783 32 322 Cuts at: 3248 3280 3498 3530 5029 5061 5103 5135 1373 Size: 32 218 32 1499 32 42 32 2626 Fragments arranged by size: 2626 1499 783 507 322 218 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2022 3732 3978 669 Size: 988 281 84 1710 246 3079 Fragments arranged by size: 3079 1710 988 281 246 84 HindIII A'AGCT_T Cuts at: 748 748 Size: 6388 HpaI GTT'AAC Cuts at: 3978 3978 Size: 6388 KasI G'GCGC_C Cuts at: 3247 3247 Size: 6388 KpnI G_GTAC'C Cuts at: 3713 3713 Size: 6388 MfeI C'AATT_G Cuts at: 3987 3987 Size: 6388 MluI A'CGCG_T Cuts at: 6264 6264 Size: 6388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2360 2691 5502 5686 1305 Size: 1055 331 2811 184 2007 Fragments arranged by size: 2811 2007 1055 331 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2858 10 Size: 54 1425 343 177 849 3540 Fragments arranged by size: 3540 1425 849 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2286 3111 3131 4346 4705 4864 518 Size: 648 1120 825 20 1215 359 159 2042 Fragments arranged by size: 2042 1215 1120 825 648 359 159 20 MspA1I CmG'CkG Cuts at: 1606 2029 2057 2354 2416 2900 3804 4481 5629 Size: 423 28 297 62 484 904 677 1148 Cuts at: 5629 5874 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 904 677 484 423 297 245 62 28 NaeI GCC'GGC Cuts at: 3246 3482 3246 Size: 236 6152 NarI GG'CG_CC Cuts at: 3248 3248 Size: 6388 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4897 Fragments arranged by size: 4897 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6388 NgoMIV G'CCGG_C Cuts at: 3244 3480 3244 Size: 236 6152 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6388 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 3746 5291 1132 Size: 97 2517 1545 2229 Fragments arranged by size: 2517 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3269 3475 3583 1348 Size: 1921 206 108 4153 Fragments arranged by size: 4153 1921 206 108 PciI A'CATG_T Cuts at: 5287 5287 Size: 6388 PflMI CCAn_nnn'nTGG Cuts at: 1108 2854 3616 1108 Size: 1746 762 3880 Fragments arranged by size: 3880 1746 762 PfoI T'CCnGG_A Cuts at: 3000 3000 Size: 6388 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4457 4489 5970 6002 4457 Size: 32 1481 32 4843 Fragments arranged by size: 4843 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2345 2409 2104 Size: 241 64 6083 Fragments arranged by size: 6083 241 64 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 3958 3958 Size: 6388 PspOMI G'GGCC_C Cuts at: 2642 2642 Size: 6388 PstI C_TGCA'G Cuts at: 830 2112 2599 3306 3357 3526 3740 830 Size: 1282 487 707 51 169 214 3478 Fragments arranged by size: 3478 1282 707 487 214 169 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 2029 2354 2416 2900 2029 Size: 325 62 484 5517 Fragments arranged by size: 5517 484 325 62 SalI G'TCGA_C Cuts at: 1936 2020 3730 1936 Size: 84 1710 4594 Fragments arranged by size: 4594 1710 84 SanDI GG'GwC_CC Cuts at: 2409 2409 Size: 6388 SbfI CC_TGCA'GG Cuts at: 3357 3740 3357 Size: 383 6005 ScaI AGT'ACT Cuts at: 4636 6348 4636 Size: 1712 4676 SfcI C'TryA_G Cuts at: 826 1045 2108 2595 3302 3353 3522 3736 4871 Size: 219 1063 487 707 51 169 214 1135 Cuts at: 4871 5552 5743 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1063 707 681 487 219 214 191 169 51 SfoI GGC'GCC Cuts at: 3249 3249 Size: 6388 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6388 SmaI CCC'GGG Cuts at: 1872 2036 2528 3715 1872 Size: 164 492 1187 4545 Fragments arranged by size: 4545 1187 492 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 4492 5393 5655 820 Size: 197 926 2549 901 262 1553 Fragments arranged by size: 2549 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4179 493 Size: 3686 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6388 SphI G_CATG'C Cuts at: 1132 3746 1132 Size: 2614 3774 SrfI GCCC'GGGC Cuts at: 2036 2036 Size: 6388 SspI AAT'ATT Cuts at: 5 52 4312 5 Size: 47 4260 2081 Fragments arranged by size: 4260 2081 47 StuI AGG'CCT Cuts at: 1517 3206 1517 Size: 1689 4699 StyI C'CwwG_G Cuts at: 513 1065 2004 2436 2817 3054 3827 513 Size: 552 939 432 381 237 773 3074 Fragments arranged by size: 3074 939 773 552 432 381 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2233 4415 4432 4585 4770 5215 325 Size: 1255 653 2182 17 153 185 445 1498 Fragments arranged by size: 2182 1498 1255 653 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3312 4634 6346 Size: 274 80 33 51 1443 1335 1322 1712 Cuts at: 6346 96 Size: 138 Fragments arranged by size: 1712 1443 1335 1322 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2126 2158 3202 3234 6348 6380 2126 Size: 32 1044 32 3114 32 2134 Fragments arranged by size: 3114 2134 1044 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3152 1316 Size: 1836 4552 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2434 2616 3613 2434 Size: 182 997 5209 Fragments arranged by size: 5209 997 182 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6388 XmaI C'CCGG_G Cuts at: 1870 2034 2526 3713 1870 Size: 164 492 1187 4545 Fragments arranged by size: 4545 1187 492 164 XmnI GAAnn'nnTTC Cuts at: 4517 4517 Size: 6388 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SwaI