(Circular) (Six-base) MAPSORT of: pFN21AB5813.seq Check: 142 from: 1 to: 6127 pFN21AB5813 6127 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2272 2555 2615 3117 3277 2272 Size: 283 60 502 160 5122 Fragments arranged by size: 5122 502 283 160 60 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2643 3937 278 Size: 53 83 186 936 1107 1294 2468 Fragments arranged by size: 2468 1294 1107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2103 3470 1937 Size: 166 1367 4594 Fragments arranged by size: 4594 1367 166 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2371 4190 5574 5842 1632 Size: 739 1819 1384 268 1917 Fragments arranged by size: 1917 1819 1384 739 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 2445 2619 3000 5026 6003 1203 Size: 1242 174 381 2026 977 1327 Fragments arranged by size: 2026 1327 1242 977 381 174 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2873 1184 Size: 1689 4438 AlwNI CAG_nnn'CTG Cuts at: 2695 3123 3870 5442 2695 Size: 428 747 1572 3380 Fragments arranged by size: 3380 1572 747 428 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 3436 3662 3814 5923 1493 Size: 1943 226 152 2109 1697 Fragments arranged by size: 2109 1943 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2304 2423 3452 1870 Size: 73 270 91 119 1029 4545 Fragments arranged by size: 4545 1029 270 119 91 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2432 2465 2432 Size: 33 6094 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2543 3105 3289 3448 4903 Size: 325 217 627 756 562 184 159 1455 Cuts at: 4903 618 Size: 1842 Fragments arranged by size: 1842 1455 756 627 562 325 217 184 159 BanII G_rGCy'C Cuts at: 1391 2334 2348 2503 2843 2970 3054 3446 1391 Size: 943 14 155 340 127 84 392 4072 Fragments arranged by size: 4072 943 392 340 155 127 84 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2635 928 Size: 971 736 4420 Fragments arranged by size: 4420 971 736 BbvCI CC'TCA_GC Cuts at: 3234 3234 Size: 6127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2150 2184 4318 4352 1903 Size: 34 213 34 2134 34 3678 Fragments arranged by size: 3678 2134 213 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2375 3103 3319 Size: 107 122 71 631 605 703 728 216 Cuts at: 3319 4738 136 Size: 1419 1525 Fragments arranged by size: 1525 1419 728 703 631 605 216 122 107 71 BglII A'GATC_T Cuts at: 3060 3325 6122 3060 Size: 265 2797 3065 Fragments arranged by size: 3065 2797 265 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 2546 2619 3108 3294 4188 5344 1790 Size: 756 73 489 186 894 1156 2573 Fragments arranged by size: 2573 1156 894 756 489 186 73 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3037 3858 4816 Size: 416 53 83 399 24 1620 821 958 Cuts at: 4816 442 Size: 1753 Fragments arranged by size: 1753 1620 958 821 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2332 2503 2921 3375 4787 1239 Size: 1046 47 171 418 454 1412 2579 Fragments arranged by size: 2579 1412 1046 454 418 171 47 Bpu10I CC'TnA_GC Cuts at: 3234 3234 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2663 4252 5117 5415 2663 Size: 1589 865 298 3375 Fragments arranged by size: 3375 1589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4790 882 Size: 617 3291 2219 Fragments arranged by size: 3291 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2620 3001 3918 493 Size: 711 1416 381 917 2702 Fragments arranged by size: 2702 1416 917 711 381 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2640 3934 4316 4951 Size: 53 83 186 936 1107 1294 382 635 Cuts at: 4951 275 Size: 1451 Fragments arranged by size: 1451 1294 1107 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2476 2506 1178 Size: 30 165 30 1073 30 4799 Fragments arranged by size: 4799 1073 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2666 3023 3294 1186 Size: 1480 357 271 4019 Fragments arranged by size: 4019 1480 357 271 BseYI C'CCAG_C Cuts at: 2798 2985 3189 3280 3354 3400 5330 5826 2798 Size: 187 204 91 74 46 1930 496 3099 Fragments arranged by size: 3099 1930 496 204 187 91 74 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2393 2554 1163 Size: 573 657 161 4736 Fragments arranged by size: 4736 657 573 161 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2199 3426 3577 4338 5366 1540 Size: 275 186 198 1227 151 761 1028 2301 Fragments arranged by size: 2301 1227 1028 761 275 198 186 151 BsiHKAI G_wGCw'C Cuts at: 2334 2843 2898 2970 3054 4188 4273 5344 2334 Size: 509 55 72 84 1134 85 1071 3117 Fragments arranged by size: 3117 1134 1071 509 85 84 72 55 BsmI GAATG_Cn' Cuts at: 3638 3731 3638 Size: 93 6034 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2334 2348 2503 2546 2619 2843 2898 Size: 399 544 14 155 43 73 224 55 Cuts at: 2898 2970 3054 3108 3294 3446 4188 4273 5344 Size: 72 84 54 186 152 742 85 1071 Cuts at: 5344 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 544 399 224 186 155 152 85 84 73 72 55 54 43 14 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2151 2272 2555 2615 3117 3167 3277 3482 Size: 1307 121 283 60 502 50 110 205 Cuts at: 3482 844 Size: 3489 Fragments arranged by size: 3489 1307 502 283 205 121 110 60 50 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2102 3308 4622 4796 65 Size: 1183 854 1206 1314 174 1396 Fragments arranged by size: 1396 1314 1206 1183 854 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2198 4771 4958 1700 Size: 110 388 2573 187 2869 Fragments arranged by size: 2869 2573 388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 3123 794 Size: 2329 3798 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6127 BstYI r'GATC_y Cuts at: 1972 3060 3325 3457 3824 4209 4226 5678 5764 Size: 1088 265 132 367 385 17 1452 86 Cuts at: 5764 5776 6122 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1088 385 367 346 265 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2024 2663 3033 3217 3317 513 Size: 552 939 20 639 370 184 100 3323 Fragments arranged by size: 3323 939 639 552 370 184 100 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 2825 3259 3643 4437 4457 2825 Size: 434 384 794 20 4495 Fragments arranged by size: 4495 794 434 384 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DraIII CAC_nnn'GTG Cuts at: 2557 2557 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2256 2286 Size: 54 1425 50 275 18 366 60 30 Cuts at: 2286 2367 2376 2666 2971 3320 3423 3574 4463 Size: 81 9 290 305 349 103 151 889 Cuts at: 4463 6092 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 366 349 305 290 275 151 103 81 60 54 50 43 30 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2196 3423 3574 1537 Size: 275 384 1227 151 4090 Fragments arranged by size: 4090 1227 384 275 151 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 2085 2339 2423 4057 1353 Size: 684 48 254 84 1634 3423 Fragments arranged by size: 3423 1634 684 254 84 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2217 4714 5088 5234 387 Size: 1064 138 628 2497 374 146 1280 Fragments arranged by size: 2497 1280 1064 628 374 146 138 EcoICRI GAG'CTC Cuts at: 2332 2841 2968 3052 2332 Size: 509 127 84 5407 Fragments arranged by size: 5407 509 127 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2332 2371 2503 2921 3375 4190 Size: 393 653 47 39 132 418 454 815 Cuts at: 4190 4787 5574 5842 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 815 787 653 597 454 418 393 268 132 47 39 EcoNI CCTnn'n_nnAGG Cuts at: 2482 2888 2482 Size: 406 5721 EcoO109I rG'GnC_Cy Cuts at: 2140 2219 2464 3404 3412 2140 Size: 79 245 940 8 4855 Fragments arranged by size: 4855 940 245 79 8 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2935 2935 Size: 6127 FspI TGC'GCA Cuts at: 2588 4633 2588 Size: 2045 4082 HaeII r_GCGC'y Cuts at: 1678 2086 5274 1678 Size: 408 3188 2531 Fragments arranged by size: 3188 2531 408 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2516 2548 4768 4800 4842 Size: 32 135 32 944 32 2220 32 42 Cuts at: 4842 4874 1373 Size: 32 2626 Fragments arranged by size: 2626 2220 944 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2104 2704 3471 3717 669 Size: 988 281 166 600 767 246 3079 Fragments arranged by size: 3079 988 767 600 281 246 166 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3255 5241 5425 1305 Size: 1950 1986 184 2007 Fragments arranged by size: 2007 1986 1950 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2258 2369 2378 2668 10 Size: 54 1425 343 426 111 9 290 3469 Fragments arranged by size: 3469 1425 426 343 290 111 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2999 4085 4444 4603 518 Size: 648 1833 1086 359 159 2042 Fragments arranged by size: 2042 1833 1086 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2164 2365 2613 2802 3016 3120 3174 3395 Size: 558 201 248 189 214 104 54 221 Cuts at: 3395 3543 4220 5368 5613 1606 Size: 148 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 558 248 245 221 214 201 189 148 104 54 NaeI GCC'GGC Cuts at: 2200 2200 Size: 6127 NcoI C'CATG_G Cuts at: 513 1065 2004 2663 3033 513 Size: 552 939 659 370 3607 Fragments arranged by size: 3607 939 659 552 370 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NgoMIV G'CCGG_C Cuts at: 2198 2198 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2398 2449 3485 5030 1132 Size: 97 1169 51 1036 1545 2229 Fragments arranged by size: 2229 1545 1169 1036 97 51 PasI CC'CwG_GG Cuts at: 1348 2056 2517 1348 Size: 708 461 4958 Fragments arranged by size: 4958 708 461 PciI A'CATG_T Cuts at: 2445 5026 2445 Size: 2581 3546 PflMI CCAn_nnn'nTGG Cuts at: 1108 2557 1108 Size: 1449 4678 PfoI T'CCnGG_A Cuts at: 2898 2898 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PmlI CAC'GTG Cuts at: 2620 3001 2620 Size: 381 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2140 2219 3404 3412 2140 Size: 79 1185 8 4855 Fragments arranged by size: 4855 1185 79 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PstI C_TGCA'G Cuts at: 830 2082 2612 2760 2768 2862 3021 3119 3479 Size: 1252 530 148 8 94 159 98 360 Cuts at: 3479 830 Size: 3478 Fragments arranged by size: 3478 1252 530 360 159 148 98 94 8 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2164 2365 2802 3016 3120 3395 2164 Size: 201 437 214 104 275 4896 Fragments arranged by size: 4896 437 275 214 201 104 SacI G_AGCT'C Cuts at: 2334 2843 2970 3054 2334 Size: 509 127 84 5407 Fragments arranged by size: 5407 509 127 84 SalI G'TCGA_C Cuts at: 1936 2102 3469 1936 Size: 166 1367 4594 Fragments arranged by size: 4594 1367 166 SanDI GG'GwC_CC Cuts at: 2219 3404 2219 Size: 1185 4942 SapI GCTCTTCn'nnn_ Cuts at: 2085 2339 2085 Size: 254 5873 SbfI CC_TGCA'GG Cuts at: 2768 3479 2768 Size: 711 5416 ScaI AGT'ACT Cuts at: 2435 4375 6087 2435 Size: 1940 1712 2475 Fragments arranged by size: 2475 1940 1712 SfcI C'TryA_G Cuts at: 826 1045 2078 2608 2756 2764 2858 3017 3115 Size: 219 1033 530 148 8 94 159 98 Cuts at: 3115 3475 4610 5291 5482 826 Size: 360 1135 681 191 1471 Fragments arranged by size: 1471 1135 1033 681 530 360 219 191 159 148 98 94 8 SfiI GGCCn_nnn'nGGCC Cuts at: 2375 2375 Size: 6127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 2215 2306 2425 3454 1872 Size: 343 91 119 1029 4545 Fragments arranged by size: 4545 1029 343 119 91 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 4231 5132 5394 820 Size: 197 926 735 1553 901 262 1553 Fragments arranged by size: 1553 1553 926 901 735 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 2762 1517 Size: 1245 4882 StyI C'CwwG_G Cuts at: 513 1065 2004 2460 2663 3033 3566 513 Size: 552 939 456 203 370 533 3074 Fragments arranged by size: 3074 939 552 533 456 370 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2724 4154 4171 4324 4509 4954 325 Size: 1255 1144 1430 17 153 185 445 1498 Fragments arranged by size: 1498 1430 1255 1144 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2433 2804 4373 Size: 274 80 33 51 1443 456 371 1569 Cuts at: 4373 6085 96 Size: 1712 138 Fragments arranged by size: 1712 1569 1443 456 371 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3238 3270 6087 6119 3238 Size: 32 2817 32 3246 Fragments arranged by size: 3246 2817 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 2213 2304 2423 3452 1870 Size: 343 91 119 1029 4545 Fragments arranged by size: 4545 1029 343 119 91 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2641 3935 276 Size: 53 83 186 936 1107 1294 2468 Fragments arranged by size: 2468 1294 1107 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI XcmI