(Circular) (Six-base) MAPSORT of: pFN21AB9936.seq Check: 2384 from: 1 to: 6607 pFN21AB9936 6607 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3765 4417 278 Size: 53 83 186 936 2229 652 2468 Fragments arranged by size: 2468 2229 936 652 186 83 53 AccI GT'mk_AC Cuts at: 1937 3950 1937 Size: 2013 4594 Acc65I G'GTAC_C Cuts at: 3253 3928 3253 Size: 675 5932 AclI AA'CG_TT Cuts at: 1563 4734 5107 1563 Size: 3171 373 3063 Fragments arranged by size: 3171 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4670 6054 6322 1632 Size: 3038 1384 268 1917 Fragments arranged by size: 3038 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6410 AflIII A'CryG_T Cuts at: 1203 3462 5506 6483 1203 Size: 2259 2044 977 1327 Fragments arranged by size: 2259 2044 1327 977 AgeI A'CCGG_T Cuts at: 5438 5438 Size: 6607 AhdI GACnn_n'nnGTC Cuts at: 3034 5336 3034 Size: 2302 4305 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6607 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3516 1184 Size: 2332 4275 AlwNI CAG_nnn'CTG Cuts at: 2155 3052 3652 4350 5922 2155 Size: 897 600 698 1572 2840 Fragments arranged by size: 2840 1572 897 698 600 ApaI G_GGCC'C Cuts at: 2168 2168 Size: 6607 ApaLI G'TGCA_C Cuts at: 2528 4664 5820 2528 Size: 2136 1156 3315 Fragments arranged by size: 3315 2136 1156 ApoI r'AATT_y Cuts at: 1493 2030 3916 4142 4294 6403 1493 Size: 537 1886 226 152 2109 1697 Fragments arranged by size: 2109 1886 1697 537 226 152 AseI AT'TA_AT Cuts at: 160 1032 5161 160 Size: 872 4129 1606 Fragments arranged by size: 4129 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 AvaI C'yCGr_G Cuts at: 1870 1943 2263 2795 3571 3932 1870 Size: 73 320 532 776 361 4545 Fragments arranged by size: 4545 776 532 361 320 73 BamHI G'GATC_C Cuts at: 3937 4304 3937 Size: 367 6240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2225 2292 2405 2879 3166 Size: 325 217 627 438 67 113 474 287 Cuts at: 3166 3253 3325 3928 5383 618 Size: 87 72 603 1455 1842 Fragments arranged by size: 1842 1455 627 603 474 438 325 287 217 113 87 72 67 BanII G_rGCy'C Cuts at: 1391 2168 2805 3083 3422 3578 3926 1391 Size: 777 637 278 339 156 348 4072 Fragments arranged by size: 4072 777 637 348 339 278 156 BbeI G_GCGC'C Cuts at: 2883 3170 3329 2883 Size: 287 159 6161 Fragments arranged by size: 6161 287 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2633 928 Size: 971 734 4902 Fragments arranged by size: 4902 971 734 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4798 4832 1903 Size: 34 2861 34 3678 Fragments arranged by size: 3678 2861 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4499 5709 3650 Size: 849 1210 4548 Fragments arranged by size: 4548 1210 849 BclI T'GATC_A Cuts at: 1548 1548 Size: 6607 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2337 2403 2931 Size: 107 122 71 631 605 665 66 528 Cuts at: 2931 3494 3618 3672 5218 136 Size: 563 124 54 1546 1525 Fragments arranged by size: 1546 1525 665 631 605 563 528 124 122 107 71 66 54 BglII A'GATC_T Cuts at: 6602 6602 Size: 6607 BlpI GC'TnA_GC Cuts at: 4024 4024 Size: 6607 Bme1580I G_kGCm'C Cuts at: 1790 2168 2532 4668 5824 1790 Size: 378 364 2136 1156 2573 Fragments arranged by size: 2573 2136 1156 378 364 BmgBI CAC'GTC Cuts at: 1318 2527 1318 Size: 1209 5398 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2143 2171 3265 Size: 416 53 83 399 24 726 28 1094 Cuts at: 3265 3662 4338 5296 442 Size: 397 676 958 1753 Fragments arranged by size: 1753 1094 958 726 676 416 399 397 83 53 28 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2123 2461 2812 3062 3538 3539 5267 Size: 878 6 338 351 250 476 1 1728 Cuts at: 5267 1239 Size: 2579 Fragments arranged by size: 2579 1728 878 476 351 338 250 6 1 Bpu10I CC'TnA_GC Cuts at: 2108 2951 2108 Size: 843 5764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3788 4732 5597 5895 3788 Size: 944 865 298 4500 Fragments arranged by size: 4500 944 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2567 5270 882 Size: 617 1068 2703 2219 Fragments arranged by size: 2703 2219 1068 617 BsaAI yAC'GTr Cuts at: 493 1204 4398 493 Size: 711 3194 2702 Fragments arranged by size: 3194 2702 711 BsaBI GATnn'nnATC Cuts at: 3308 3314 6601 3308 Size: 6 3287 3314 Fragments arranged by size: 3314 3287 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2486 2880 2934 2968 Size: 53 83 186 936 953 394 54 34 Cuts at: 2968 3030 3167 3326 3762 4414 4796 5431 275 Size: 62 137 159 436 652 382 635 1451 Fragments arranged by size: 1451 953 936 652 635 436 394 382 186 159 137 83 62 54 53 34 BsaWI w'CCGG_w Cuts at: 3891 5040 5438 5712 5859 6270 3891 Size: 1149 398 274 147 411 4228 Fragments arranged by size: 4228 1149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 1178 Size: 30 165 30 1350 30 5002 Fragments arranged by size: 5002 1350 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2105 2813 2899 3111 1186 Size: 901 18 708 86 212 4682 Fragments arranged by size: 4682 901 708 212 86 18 BseYI C'CCAG_C Cuts at: 2168 2926 3084 3105 3135 3203 3716 3871 5810 Size: 758 158 21 30 68 513 155 1939 Cuts at: 5810 6306 2168 Size: 496 2469 Fragments arranged by size: 2469 1939 758 513 496 158 155 68 30 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 2453 2799 3818 1163 Size: 573 300 417 346 1019 3952 Fragments arranged by size: 3952 1019 573 417 346 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4057 4818 5846 1540 Size: 275 186 2056 761 1028 2301 Fragments arranged by size: 2301 2056 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2480 2532 2900 2957 3422 4668 4753 5824 2480 Size: 52 368 57 465 1246 85 1071 3263 Fragments arranged by size: 3263 1246 1071 465 368 85 57 52 BsmI GAATG_Cn' Cuts at: 4118 4211 4118 Size: 93 6514 BsmBI CGTCTCn'nnnn_ Cuts at: 2069 2069 Size: 6607 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2480 2532 2805 2900 2957 3083 Size: 399 378 312 52 273 95 57 126 Cuts at: 3083 3422 3578 3926 4668 4753 5824 1391 Size: 339 156 348 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 378 348 339 312 273 156 126 95 85 57 52 BspEI T'CCGG_A Cuts at: 3891 6270 3891 Size: 2379 4228 BspHI T'CATG_A Cuts at: 2097 4496 6226 2097 Size: 2399 1730 2478 Fragments arranged by size: 2478 2399 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 2244 3962 844 Size: 1319 81 1718 3489 Fragments arranged by size: 3489 1718 1319 81 BsrBI CCG'CTC Cuts at: 1394 1823 2776 4494 1394 Size: 429 953 1718 3507 Fragments arranged by size: 3507 1718 953 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5102 5276 65 Size: 1183 3854 174 1396 Fragments arranged by size: 3854 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2331 2352 2837 2882 3328 3356 5251 Size: 110 521 21 485 45 446 28 1895 Cuts at: 5251 5438 1700 Size: 187 2869 Fragments arranged by size: 2869 1895 521 485 446 187 110 45 28 21 BsrGI T'GTAC_A Cuts at: 96 3564 96 Size: 3468 3139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6607 BssSI C'ACGA_G Cuts at: 3219 4667 5679 3219 Size: 1448 1012 4147 Fragments arranged by size: 4147 1448 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3052 794 Size: 2258 4349 BstBI TT'CG_AA Cuts at: 2015 5411 2015 Size: 3396 3211 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6607 BstXI CCAn_nnnn'nTGG Cuts at: 2186 3745 2186 Size: 1559 5048 BstYI r'GATC_y Cuts at: 1972 3937 4304 4689 4706 6158 6244 6256 6602 Size: 1965 367 385 17 1452 86 12 346 Cuts at: 6602 1972 Size: 1977 Fragments arranged by size: 1977 1965 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2024 2229 2296 2427 3024 3625 Size: 552 939 20 205 67 131 597 601 Cuts at: 3625 513 Size: 3495 Fragments arranged by size: 3495 939 601 597 552 205 131 67 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2964 3301 487 Size: 683 56 1738 337 3793 Fragments arranged by size: 3793 1738 683 337 56 BtsI GCAGTG_nn' Cuts at: 2518 4123 4917 4937 2518 Size: 1605 794 20 4188 Fragments arranged by size: 4188 1605 794 20 ClaI AT'CG_AT Cuts at: 1554 3302 1554 Size: 1748 4859 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6572 DraI TTT'AAA Cuts at: 3911 3911 Size: 6607 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3706 5614 809 Size: 507 2390 1908 1802 Fragments arranged by size: 2390 1908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2329 2850 2884 Size: 54 1425 50 275 18 499 521 34 Cuts at: 2884 3292 3366 3486 3600 3747 3864 4054 4943 Size: 408 74 120 114 147 117 190 889 Cuts at: 4943 6572 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 521 499 408 275 190 147 120 117 114 74 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 4054 1537 Size: 275 2242 4090 Fragments arranged by size: 4090 2242 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2565 4537 1353 Size: 1212 1972 3423 Fragments arranged by size: 3423 1972 1212 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2084 2452 3164 5194 5568 5714 Size: 1064 138 495 368 712 2030 374 146 Cuts at: 5714 387 Size: 1280 Fragments arranged by size: 2030 1280 1064 712 495 374 368 146 138 EcoICRI GAG'CTC Cuts at: 3420 3420 Size: 6607 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2123 2461 2812 3062 3538 3539 Size: 393 485 6 338 351 250 476 1 Cuts at: 3539 4670 5267 6054 6322 1239 Size: 1131 597 787 268 1524 Fragments arranged by size: 1524 1131 787 597 485 476 393 351 338 268 250 6 1 EcoO109I rG'GnC_Cy Cuts at: 2104 2165 2193 2517 2656 2736 2104 Size: 61 28 324 139 80 5975 Fragments arranged by size: 5975 324 139 80 61 28 EcoRI G'AATT_C Cuts at: 3916 4294 3916 Size: 378 6229 FspI TGC'GCA Cuts at: 5113 5113 Size: 6607 HaeII r_GCGC'y Cuts at: 1678 2883 3170 3210 3329 3357 5754 1678 Size: 1205 287 40 119 28 2397 2531 Fragments arranged by size: 2531 2397 1205 287 119 40 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 3020 3052 3305 Size: 32 135 32 992 32 424 32 253 Cuts at: 3305 3337 3727 3759 5248 5280 5322 5354 1373 Size: 32 390 32 1489 32 42 32 2626 Fragments arranged by size: 2626 1489 992 424 390 253 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2553 3951 4197 669 Size: 988 281 615 1398 246 3079 Fragments arranged by size: 3079 1398 988 615 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6607 HpaI GTT'AAC Cuts at: 2553 4197 2553 Size: 1644 4963 KasI G'GCGC_C Cuts at: 2879 3166 3325 2879 Size: 287 159 6161 Fragments arranged by size: 6161 287 159 KpnI G_GTAC'C Cuts at: 3257 3932 3257 Size: 675 5932 MfeI C'AATT_G Cuts at: 4206 4206 Size: 6607 MluI A'CGCG_T Cuts at: 3462 6483 3462 Size: 3021 3586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3801 5721 5905 1305 Size: 2496 1920 184 2007 Fragments arranged by size: 2496 2007 1920 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3294 10 Size: 54 1425 343 1462 3323 Fragments arranged by size: 3323 1462 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4565 4924 5083 518 Size: 648 3399 359 159 2042 Fragments arranged by size: 3399 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2394 2429 2439 2788 2930 2998 3052 Size: 420 368 35 10 349 142 68 54 Cuts at: 3052 3517 4023 4700 5848 6093 1606 Size: 465 506 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 506 465 420 368 349 245 142 68 54 35 10 NaeI GCC'GGC Cuts at: 2333 2354 2884 3330 3358 2333 Size: 21 530 446 28 5582 Fragments arranged by size: 5582 530 446 28 21 NarI GG'CG_CC Cuts at: 2880 3167 3326 2880 Size: 287 159 6161 Fragments arranged by size: 6161 287 159 NcoI C'CATG_G Cuts at: 513 1065 2004 2229 2296 3024 513 Size: 552 939 225 67 728 4096 Fragments arranged by size: 4096 939 728 552 225 67 NdeI CA'TA_TG Cuts at: 387 387 Size: 6607 NgoMIV G'CCGG_C Cuts at: 2331 2352 2882 3328 3356 2331 Size: 21 530 446 28 5582 Fragments arranged by size: 5582 530 446 28 21 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6607 NotI GC'GGCC_GC Cuts at: 4054 4054 Size: 6607 NspI r_CATG'y Cuts at: 1132 1229 3841 3965 5510 1132 Size: 97 2612 124 1545 2229 Fragments arranged by size: 2612 2229 1545 124 97 PasI CC'CwG_GG Cuts at: 1348 3413 3442 1348 Size: 2065 29 4513 Fragments arranged by size: 4513 2065 29 PciI A'CATG_T Cuts at: 5506 5506 Size: 6607 PflMI CCAn_nnn'nTGG Cuts at: 1108 2302 3517 1108 Size: 1194 1215 4198 Fragments arranged by size: 4198 1215 1194 PmeI GTTT'AAAC Cuts at: 3911 3911 Size: 6607 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4676 4708 6189 6221 4676 Size: 32 1481 32 5062 Fragments arranged by size: 5062 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2193 2517 2656 2736 2104 Size: 89 324 139 80 5975 Fragments arranged by size: 5975 324 139 89 80 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6607 PsiI TTA'TAA Cuts at: 4177 4177 Size: 6607 PspOMI G'GGCC_C Cuts at: 2164 2164 Size: 6607 PstI C_TGCA'G Cuts at: 830 3339 3959 830 Size: 2509 620 3478 Fragments arranged by size: 3478 2509 620 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6607 PvuII CAG'CTG Cuts at: 2394 2439 2930 3052 3517 2394 Size: 45 491 122 465 5484 Fragments arranged by size: 5484 491 465 122 45 SacI G_AGCT'C Cuts at: 3422 3422 Size: 6607 SacII CC_GC'GG Cuts at: 2027 2430 2027 Size: 403 6204 SalI G'TCGA_C Cuts at: 1936 3949 1936 Size: 2013 4594 SanDI GG'GwC_CC Cuts at: 2193 2517 2736 2193 Size: 324 219 6064 Fragments arranged by size: 6064 324 219 SbfI CC_TGCA'GG Cuts at: 3959 3959 Size: 6607 ScaI AGT'ACT Cuts at: 2183 4855 6567 2183 Size: 2672 1712 2223 Fragments arranged by size: 2672 2223 1712 SfcI C'TryA_G Cuts at: 826 1045 2700 3018 3335 3955 5090 5771 5962 Size: 219 1655 318 317 620 1135 681 191 Cuts at: 5962 826 Size: 1471 Fragments arranged by size: 1655 1471 1135 681 620 318 317 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3494 3494 Size: 6607 SfoI GGC'GCC Cuts at: 2881 3168 3327 2881 Size: 287 159 6161 Fragments arranged by size: 6161 287 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6607 SmaI CCC'GGG Cuts at: 1872 3573 3934 1872 Size: 1701 361 4545 Fragments arranged by size: 4545 1701 361 SmlI C'TyrA_G Cuts at: 820 1017 1943 3803 4711 5612 5874 820 Size: 197 926 1860 908 901 262 1553 Fragments arranged by size: 1860 1553 926 908 901 262 197 SnaBI TAC'GTA Cuts at: 493 4398 493 Size: 3905 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6607 SphI G_CATG'C Cuts at: 1132 3965 1132 Size: 2833 3774 SrfI GCCC'GGGC Cuts at: 3573 3573 Size: 6607 SspI AAT'ATT Cuts at: 5 52 4531 5 Size: 47 4479 2081 Fragments arranged by size: 4479 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6607 StyI C'CwwG_G Cuts at: 513 1065 2004 2229 2296 3024 4046 513 Size: 552 939 225 67 728 1022 3074 Fragments arranged by size: 3074 1022 939 728 552 225 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2735 3019 3086 4634 4651 4804 4989 Size: 1255 1155 284 67 1548 17 153 185 Cuts at: 4989 5434 325 Size: 445 1498 Fragments arranged by size: 1548 1498 1255 1155 445 284 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3564 4853 Size: 274 80 33 51 1443 204 1383 1289 Cuts at: 4853 6565 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1383 1289 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2753 2785 6567 6599 2753 Size: 32 3782 32 2761 Fragments arranged by size: 3782 2761 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2632 1316 Size: 1316 5291 XbaI T'CTAG_A Cuts at: 3943 3943 Size: 6607 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2219 2219 Size: 6607 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6607 XmaI C'CCGG_G Cuts at: 1870 3571 3932 1870 Size: 1701 361 4545 Fragments arranged by size: 4545 1701 361 XmnI GAAnn'nnTTC Cuts at: 4736 4736 Size: 6607 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3763 4415 276 Size: 53 83 186 936 2229 652 2468 Fragments arranged by size: 2468 2229 936 652 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SexAI SwaI