(Circular) (Six-base) MAPSORT of: pFN21AB7992.seq Check: 770 from: 1 to: 6010 pFN21AB7992 6010 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3820 278 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3353 1937 Size: 1416 4594 Acc65I G'GTAC_C Cuts at: 2762 3331 2762 Size: 569 5441 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 2233 2712 3064 4073 5457 5725 1632 Size: 476 125 479 352 1009 1384 268 1917 Fragments arranged by size: 1917 1384 1009 479 476 352 268 125 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 4909 5886 1203 Size: 3706 977 1327 Fragments arranged by size: 3706 1327 977 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 2277 4739 2277 Size: 2462 3548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6010 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6010 AlwNI CAG_nnn'CTG Cuts at: 2108 2359 2513 3753 5325 2108 Size: 251 154 1240 1572 2793 Fragments arranged by size: 2793 1572 1240 251 154 ApaI G_GGCC'C Cuts at: 2034 2034 Size: 6010 ApaLI G'TGCA_C Cuts at: 4067 5223 4067 Size: 1156 4854 ApoI r'AATT_y Cuts at: 1493 2862 3030 3198 3319 3545 3697 5806 1493 Size: 1369 168 168 121 226 152 2109 1697 Fragments arranged by size: 2109 1697 1369 226 168 168 152 121 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 2283 3335 1870 Size: 73 340 1052 4545 Fragments arranged by size: 4545 1052 340 73 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2762 3331 4786 618 Size: 325 217 627 975 569 1455 1842 Fragments arranged by size: 1842 1455 975 627 569 325 217 BanII G_rGCy'C Cuts at: 1391 2034 2409 2655 3008 3267 3329 1391 Size: 643 375 246 353 259 62 4072 Fragments arranged by size: 4072 643 375 353 259 246 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5039 BbvCI CC'TCA_GC Cuts at: 2327 2327 Size: 6010 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2295 2329 4201 4235 1903 Size: 34 358 34 1872 34 3678 Fragments arranged by size: 3678 1872 358 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3902 5112 3902 Size: 1210 4800 BclI T'GATC_A Cuts at: 1548 1548 Size: 6010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4621 136 Size: 107 122 71 631 605 2949 1525 Fragments arranged by size: 2949 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2232 6005 2232 Size: 3773 2237 BlpI GC'TnA_GC Cuts at: 3427 3427 Size: 6010 Bme1580I G_kGCm'C Cuts at: 1790 2034 4071 5227 1790 Size: 244 2037 1156 2573 Fragments arranged by size: 2573 2037 1156 244 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3741 4699 442 Size: 416 53 83 399 24 2324 958 1753 Fragments arranged by size: 2324 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 2486 2579 2976 4670 1239 Size: 1142 105 93 397 1694 2579 Fragments arranged by size: 2579 1694 1142 397 105 93 Bpu10I CC'TnA_GC Cuts at: 2327 2327 Size: 6010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2314 4135 5000 5298 2314 Size: 1821 865 298 3026 Fragments arranged by size: 3026 1821 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2189 2600 4673 882 Size: 617 690 411 2073 2219 Fragments arranged by size: 2219 2073 690 617 411 BsaAI yAC'GTr Cuts at: 493 1204 3801 493 Size: 711 2597 2702 Fragments arranged by size: 2702 2597 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3817 4199 4834 275 Size: 53 83 186 936 2284 382 635 1451 Fragments arranged by size: 2284 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4443 4841 5115 5262 5673 4443 Size: 398 274 147 411 4780 Fragments arranged by size: 4780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5785 Fragments arranged by size: 5785 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2417 2462 1186 Size: 1231 45 4734 Fragments arranged by size: 4734 1231 45 BseYI C'CCAG_C Cuts at: 2610 5213 5709 2610 Size: 2603 496 2911 Fragments arranged by size: 2911 2603 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2096 2499 2511 1163 Size: 573 360 403 12 4662 Fragments arranged by size: 4662 573 403 360 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3460 4221 5249 1540 Size: 275 186 1459 761 1028 2301 Fragments arranged by size: 2301 1459 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2409 3008 3094 3260 3267 4071 4156 5227 2409 Size: 599 86 166 7 804 85 1071 3192 Fragments arranged by size: 3192 1071 804 599 166 86 85 7 BsmI GAATG_Cn' Cuts at: 3521 3614 3521 Size: 93 5917 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2409 2655 3008 3094 3260 3267 Size: 399 244 375 246 353 86 166 7 Cuts at: 3267 3329 4071 4156 5227 1391 Size: 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 375 353 246 244 166 86 85 62 7 BspEI T'CCGG_A Cuts at: 5673 5673 Size: 6010 BspHI T'CATG_A Cuts at: 3899 5629 3899 Size: 1730 4280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2491 2533 3365 844 Size: 1647 42 832 3489 Fragments arranged by size: 3489 1647 832 42 BsrBI CCG'CTC Cuts at: 1394 1823 3897 1394 Size: 429 2074 3507 Fragments arranged by size: 3507 2074 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2669 4505 4679 65 Size: 1183 1421 1836 174 1396 Fragments arranged by size: 1836 1421 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4654 4841 1700 Size: 110 2844 187 2869 Fragments arranged by size: 2869 2844 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 4070 5082 4070 Size: 1012 4998 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6010 BstBI TT'CG_AA Cuts at: 3054 4814 3054 Size: 1760 4250 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstYI r'GATC_y Cuts at: 1972 2232 2355 3340 3707 4092 4109 5561 5647 Size: 260 123 985 367 385 17 1452 86 Cuts at: 5647 5659 6005 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 985 385 367 346 260 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2400 2601 2400 Size: 201 5809 BtgI C'CryG_G Cuts at: 513 1065 2004 2540 513 Size: 552 939 536 3983 Fragments arranged by size: 3983 939 552 536 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 3526 4320 4340 3526 Size: 794 20 5196 Fragments arranged by size: 5196 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 3314 3314 Size: 6010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5017 809 Size: 507 3701 1802 Fragments arranged by size: 3701 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3457 4346 5975 Size: 54 1425 50 275 18 1627 889 1629 Cuts at: 5975 8 Size: 43 Fragments arranged by size: 1629 1627 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3457 1537 Size: 275 1645 4090 Fragments arranged by size: 4090 1645 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2166 2529 3940 1353 Size: 813 363 1411 3423 Fragments arranged by size: 3423 1411 813 363 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2228 4597 4971 5117 387 Size: 1064 138 639 2369 374 146 1280 Fragments arranged by size: 2369 1280 1064 639 374 146 138 EcoICRI GAG'CTC Cuts at: 2407 3006 3265 2407 Size: 599 259 5152 Fragments arranged by size: 5152 599 259 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2233 2381 2486 2579 2712 2976 Size: 393 476 125 148 105 93 133 264 Cuts at: 2976 3064 4073 4670 5457 5725 1239 Size: 88 1009 597 787 268 1524 Fragments arranged by size: 1524 1009 787 597 476 393 268 264 148 133 125 105 93 88 EcoNI CCTnn'n_nnAGG Cuts at: 2399 2399 Size: 6010 EcoO109I rG'GnC_Cy Cuts at: 2030 2031 2503 2510 2030 Size: 1 472 7 5530 Fragments arranged by size: 5530 472 7 1 EcoRI G'AATT_C Cuts at: 3030 3198 3319 3697 3030 Size: 168 121 378 5343 Fragments arranged by size: 5343 378 168 121 FspI TGC'GCA Cuts at: 4516 4516 Size: 6010 HaeII r_GCGC'y Cuts at: 1678 5157 1678 Size: 3479 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2263 2295 2396 Size: 32 135 32 593 32 66 32 101 Cuts at: 2396 2428 2651 2683 4651 4683 4725 4757 1373 Size: 32 223 32 1968 32 42 32 2626 Fragments arranged by size: 2626 1968 593 223 135 101 66 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3354 3600 669 Size: 988 281 1416 246 3079 Fragments arranged by size: 3079 1416 988 281 246 HindIII A'AGCT_T Cuts at: 748 3243 748 Size: 2495 3515 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KpnI G_GTAC'C Cuts at: 2766 3335 2766 Size: 569 5441 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5124 5308 1305 Size: 3819 184 2007 Fragments arranged by size: 3819 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4188 Fragments arranged by size: 4188 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3968 4327 4486 518 Size: 648 2802 359 159 2042 Fragments arranged by size: 2802 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2108 3426 4103 5251 5496 1606 Size: 443 59 1318 677 1148 245 2120 Fragments arranged by size: 2120 1318 1148 677 443 245 59 NcoI C'CATG_G Cuts at: 513 1065 2004 2540 513 Size: 552 939 536 3983 Fragments arranged by size: 3983 939 552 536 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 3368 4913 1132 Size: 97 2139 1545 2229 Fragments arranged by size: 2229 2139 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6010 PciI A'CATG_T Cuts at: 4909 4909 Size: 6010 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6010 PfoI T'CCnGG_A Cuts at: 2358 2358 Size: 6010 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2503 2503 Size: 6010 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 2919 3580 2919 Size: 661 5349 PspOMI G'GGCC_C Cuts at: 2030 2030 Size: 6010 PstI C_TGCA'G Cuts at: 830 2068 2778 3362 830 Size: 1238 710 584 3478 Fragments arranged by size: 3478 1238 710 584 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2049 2108 2049 Size: 59 5951 SacI G_AGCT'C Cuts at: 2409 3008 3267 2409 Size: 599 259 5152 Fragments arranged by size: 5152 599 259 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SapI GCTCTTCn'nnn_ Cuts at: 2529 2529 Size: 6010 SbfI CC_TGCA'GG Cuts at: 3362 3362 Size: 6010 ScaI AGT'ACT Cuts at: 2310 4258 5970 2310 Size: 1948 1712 2350 Fragments arranged by size: 2350 1948 1712 SfcI C'TryA_G Cuts at: 826 1045 2064 2774 3358 4493 5174 5365 826 Size: 219 1019 710 584 1135 681 191 1471 Fragments arranged by size: 1471 1135 1019 710 681 584 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 2285 3337 1872 Size: 413 1052 4545 Fragments arranged by size: 4545 1052 413 SmlI C'TyrA_G Cuts at: 820 1017 1943 2293 4114 5015 5277 820 Size: 197 926 350 1821 901 262 1553 Fragments arranged by size: 1821 1553 926 901 350 262 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 3934 5 Size: 47 3882 2081 Fragments arranged by size: 3882 2081 47 StuI AGG'CCT Cuts at: 1517 2291 3077 3161 1517 Size: 774 786 84 4366 Fragments arranged by size: 4366 786 774 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2540 2655 3449 513 Size: 552 939 536 115 794 3074 Fragments arranged by size: 3074 939 794 552 536 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2407 2698 3005 4037 4054 4207 4392 Size: 1255 827 291 307 1032 17 153 185 Cuts at: 4392 4837 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1032 827 445 307 291 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2308 4256 5968 Size: 274 80 33 51 1443 331 1948 1712 Cuts at: 5968 96 Size: 138 Fragments arranged by size: 1948 1712 1443 331 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2571 2603 5970 6002 2571 Size: 32 3367 32 2579 Fragments arranged by size: 3367 2579 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6010 XbaI T'CTAG_A Cuts at: 3346 3346 Size: 6010 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2273 2692 2273 Size: 419 5591 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 2283 3335 1870 Size: 413 1052 4545 Fragments arranged by size: 4545 1052 413 XmnI GAAnn'nnTTC Cuts at: 2993 3050 3077 4139 2993 Size: 57 27 1062 4864 Fragments arranged by size: 4864 1062 57 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3818 276 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI