(Circular) (Six-base) MAPSORT of: pFN21AB9934.seq Check: 4809 from: 1 to: 6646 pFN21AB9934 6646 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2599 3194 2599 Size: 595 6051 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4456 278 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3989 1937 Size: 2052 4594 Acc65I G'GTAC_C Cuts at: 3967 3967 Size: 6646 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4709 6093 6361 1632 Size: 3077 1384 268 1917 Fragments arranged by size: 3077 1917 1384 268 AfeI AGC'GCT Cuts at: 2273 2273 Size: 6646 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6449 AflIII A'CryG_T Cuts at: 1203 2332 5545 6522 1203 Size: 1129 3213 977 1327 Fragments arranged by size: 3213 1327 1129 977 AgeI A'CCGG_T Cuts at: 2647 5477 2647 Size: 2830 3816 AhdI GACnn_n'nnGTC Cuts at: 5375 5375 Size: 6646 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6646 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2952 1184 Size: 1768 4878 AlwNI CAG_nnn'CTG Cuts at: 2128 2389 2554 2686 3382 3403 4389 5961 2128 Size: 261 165 132 696 21 986 1572 2813 Fragments arranged by size: 2813 1572 986 696 261 165 132 21 ApaI G_GGCC'C Cuts at: 2160 2160 Size: 6646 ApaLI G'TGCA_C Cuts at: 4703 5859 4703 Size: 1156 5490 ApoI r'AATT_y Cuts at: 1493 3955 4181 4333 6442 1493 Size: 2462 226 152 2109 1697 Fragments arranged by size: 2462 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5200 160 Size: 872 4168 1606 Fragments arranged by size: 4168 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 2567 3302 3971 1870 Size: 73 624 735 669 4545 Fragments arranged by size: 4545 735 669 624 73 BamHI G'GATC_C Cuts at: 3976 4343 3976 Size: 367 6279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2813 3021 3206 3227 3317 Size: 325 217 627 1026 208 185 21 90 Cuts at: 3317 3802 3967 5422 618 Size: 485 165 1455 1842 Fragments arranged by size: 1842 1455 1026 627 485 325 217 208 185 165 90 21 BanII G_rGCy'C Cuts at: 1391 2160 2292 2375 3342 3458 3522 3965 1391 Size: 769 132 83 967 116 64 443 4072 Fragments arranged by size: 4072 967 769 443 132 116 83 64 BbeI G_GCGC'C Cuts at: 3231 3231 Size: 6646 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2055 928 Size: 971 156 5519 Fragments arranged by size: 5519 971 156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4837 4871 1903 Size: 34 2900 34 3678 Fragments arranged by size: 3678 2900 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3830 4538 5748 3830 Size: 708 1210 4728 Fragments arranged by size: 4728 1210 708 BclI T'GATC_A Cuts at: 1548 1548 Size: 6646 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2105 2804 5257 Size: 107 122 71 631 605 433 699 2453 Cuts at: 5257 136 Size: 1525 Fragments arranged by size: 2453 1525 699 631 605 433 122 107 71 BglII A'GATC_T Cuts at: 3132 6641 3132 Size: 3509 3137 BlpI GC'TnA_GC Cuts at: 2523 2954 4063 2523 Size: 431 1109 5106 Fragments arranged by size: 5106 1109 431 Bme1580I G_kGCm'C Cuts at: 1790 2160 2818 3807 4707 5863 1790 Size: 370 658 989 900 1156 2573 Fragments arranged by size: 2573 1156 989 900 658 370 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6646 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4377 5335 442 Size: 416 53 83 399 24 2960 958 1753 Fragments arranged by size: 2960 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6646 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2917 3472 2917 Size: 555 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2726 3295 3446 3467 3764 3899 5306 Size: 815 672 569 151 21 297 135 1407 Cuts at: 5306 1239 Size: 2579 Fragments arranged by size: 2579 1407 815 672 569 297 151 135 21 Bpu10I CC'TnA_GC Cuts at: 3295 3295 Size: 6646 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4771 5636 5934 4771 Size: 865 298 5483 Fragments arranged by size: 5483 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2030 2447 2550 2826 3082 3321 5309 Size: 617 531 417 103 276 256 239 1988 Cuts at: 5309 882 Size: 2219 Fragments arranged by size: 2219 1988 617 531 417 276 256 239 103 BsaAI yAC'GTr Cuts at: 493 1204 2479 3496 3910 4437 493 Size: 711 1275 1017 414 527 2702 Fragments arranged by size: 2702 1275 1017 711 527 414 BsaBI GATnn'nnATC Cuts at: 6640 6640 Size: 6646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3228 4453 4835 5470 Size: 53 83 186 936 1695 1225 382 635 Cuts at: 5470 275 Size: 1451 Fragments arranged by size: 1695 1451 1225 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2647 3348 3822 5079 5477 5751 5898 6309 2647 Size: 701 474 1257 398 274 147 411 2984 Fragments arranged by size: 2984 1257 701 474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2492 2522 1178 Size: 30 165 30 624 30 435 30 5302 Fragments arranged by size: 5302 624 435 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2081 3611 3614 3644 3896 3899 3917 Size: 837 58 1530 3 30 252 3 18 Cuts at: 3917 1186 Size: 3915 Fragments arranged by size: 3915 1530 837 252 58 30 18 3 3 BseYI C'CCAG_C Cuts at: 2121 2639 2668 3489 3523 3738 5849 6345 2121 Size: 518 29 821 34 215 2111 496 2422 Fragments arranged by size: 2422 2111 821 518 496 215 34 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2011 2431 3657 3932 1163 Size: 573 275 420 1226 275 3877 Fragments arranged by size: 3877 1226 573 420 275 275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 2328 4096 4857 5885 1540 Size: 275 186 111 216 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 216 186 111 BsiHKAI G_wGCw'C Cuts at: 2375 3342 3458 3909 4707 4792 5863 2375 Size: 967 116 451 798 85 1071 3158 Fragments arranged by size: 3158 1071 967 798 451 116 85 BsmI GAATG_Cn' Cuts at: 4157 4250 4157 Size: 93 6553 BsmBI CGTCTCn'nnnn_ Cuts at: 3719 3719 Size: 6646 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2292 2375 2818 3342 3458 3522 Size: 399 370 132 83 443 524 116 64 Cuts at: 3522 3807 3909 3965 4707 4792 5863 1391 Size: 285 102 56 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 524 443 399 370 285 132 116 102 85 83 64 56 BspEI T'CCGG_A Cuts at: 3348 3822 6309 3348 Size: 474 2487 3685 Fragments arranged by size: 3685 2487 474 BspHI T'CATG_A Cuts at: 4535 6265 4535 Size: 1730 4916 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2599 3194 4001 844 Size: 1755 595 807 3489 Fragments arranged by size: 3489 1755 807 595 BsrBI CCG'CTC Cuts at: 1394 1823 2013 2077 2286 2527 3634 4533 1394 Size: 429 190 64 209 241 1107 899 3507 Fragments arranged by size: 3507 1107 899 429 241 209 190 64 BsrDI GCAATG_nn' Cuts at: 65 1248 3249 5141 5315 65 Size: 1183 2001 1892 174 1396 Fragments arranged by size: 2001 1892 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2647 2798 2835 5290 5477 1700 Size: 110 837 151 37 2455 187 2869 Fragments arranged by size: 2869 2455 837 187 151 110 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 2093 4706 5718 2093 Size: 2613 1012 3021 Fragments arranged by size: 3021 2613 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6646 BstBI TT'CG_AA Cuts at: 5450 5450 Size: 6646 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6646 BstXI CCAn_nnnn'nTGG Cuts at: 3700 3700 Size: 6646 BstYI r'GATC_y Cuts at: 1972 2490 3132 3976 4343 4728 4745 6197 6283 Size: 518 642 844 367 385 17 1452 86 Cuts at: 6283 6295 6641 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 844 642 518 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2175 2175 Size: 6646 BtgI C'CryG_G Cuts at: 513 1065 2298 2452 3331 3474 3807 513 Size: 552 1233 154 879 143 333 3352 Fragments arranged by size: 3352 1233 879 552 333 154 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5907 Fragments arranged by size: 5907 683 56 BtsI GCAGTG_nn' Cuts at: 3592 4162 4956 4976 3592 Size: 570 794 20 5262 Fragments arranged by size: 5262 794 570 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6611 DraI TTT'AAA Cuts at: 3950 3950 Size: 6646 DraIII CAC_nnn'GTG Cuts at: 2479 2479 Size: 6646 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3779 5653 809 Size: 507 2463 1874 1802 Fragments arranged by size: 2463 1874 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2161 2325 Size: 54 1425 50 275 18 279 52 164 Cuts at: 2325 2613 2656 2761 2805 3463 3681 4093 4982 Size: 288 43 105 44 658 218 412 889 Cuts at: 4982 6611 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 658 412 288 279 275 218 164 105 54 52 50 44 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2109 2325 4093 1537 Size: 275 297 216 1768 4090 Fragments arranged by size: 4090 1768 297 275 216 EarI CTCTTCn'nnn_ Cuts at: 1353 2225 3446 4576 1353 Size: 872 1221 1130 3423 Fragments arranged by size: 3423 1221 1130 872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2107 2207 5233 5607 5753 387 Size: 1064 138 518 100 3026 374 146 1280 Fragments arranged by size: 3026 1280 1064 518 374 146 138 100 EcoICRI GAG'CTC Cuts at: 2373 3340 3456 2373 Size: 967 116 5563 Fragments arranged by size: 5563 967 116 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2726 3295 3446 3467 3764 3899 Size: 393 422 672 569 151 21 297 135 Cuts at: 3899 4709 5306 6093 6361 1239 Size: 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 672 597 569 422 393 297 268 151 135 21 EcoNI CCTnn'n_nnAGG Cuts at: 2807 2930 3201 2807 Size: 123 271 6252 Fragments arranged by size: 6252 271 123 EcoO109I rG'GnC_Cy Cuts at: 2551 2749 2896 2944 3004 3299 3306 2551 Size: 198 147 48 60 295 7 5891 Fragments arranged by size: 5891 295 198 147 60 48 7 EcoRI G'AATT_C Cuts at: 3955 4333 3955 Size: 378 6268 EcoRV GAT'ATC Cuts at: 3925 3925 Size: 6646 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2527 2527 Size: 6646 FspI TGC'GCA Cuts at: 5152 5152 Size: 6646 HaeII r_GCGC'y Cuts at: 1678 2083 2275 2979 3231 5793 1678 Size: 405 192 704 252 2562 2531 Fragments arranged by size: 2562 2531 704 405 252 192 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3255 3287 3343 3375 5287 Size: 32 135 32 1683 32 56 32 1912 Cuts at: 5287 5319 5361 5393 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1912 1683 135 56 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2500 3990 4236 669 Size: 988 281 562 1490 246 3079 Fragments arranged by size: 3079 1490 988 562 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6646 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KasI G'GCGC_C Cuts at: 3227 3227 Size: 6646 KpnI G_GTAC'C Cuts at: 3971 3971 Size: 6646 MfeI C'AATT_G Cuts at: 4245 4245 Size: 6646 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5760 5944 1305 Size: 4455 184 2007 Fragments arranged by size: 4455 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2658 3465 3683 10 Size: 54 1425 343 826 807 218 2973 Fragments arranged by size: 2973 1425 826 807 343 218 54 MslI CAynn'nnrTG Cuts at: 518 1166 2061 4604 4963 5122 518 Size: 648 895 2543 359 159 2042 Fragments arranged by size: 2543 2042 895 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2300 2668 3315 3324 3622 3742 3769 Size: 522 172 368 647 9 298 120 27 Cuts at: 3769 4062 4739 5887 6132 1606 Size: 293 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 647 522 368 298 293 245 172 120 27 9 NaeI GCC'GGC Cuts at: 2800 2800 Size: 6646 NarI GG'CG_CC Cuts at: 3228 3228 Size: 6646 NcoI C'CATG_G Cuts at: 513 1065 2452 513 Size: 552 1387 4707 Fragments arranged by size: 4707 1387 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6646 NgoMIV G'CCGG_C Cuts at: 2798 2798 Size: 6646 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6646 NotI GC'GGCC_GC Cuts at: 2109 4093 2109 Size: 1984 4662 NspI r_CATG'y Cuts at: 1132 1229 4004 5549 1132 Size: 97 2775 1545 2229 Fragments arranged by size: 2775 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2771 1348 Size: 1423 5223 PciI A'CATG_T Cuts at: 5545 5545 Size: 6646 PflMI CCAn_nnn'nTGG Cuts at: 1108 2389 2611 1108 Size: 1281 222 5143 Fragments arranged by size: 5143 1281 222 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PmlI CAC'GTG Cuts at: 2479 3910 2479 Size: 1431 5215 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4715 4747 6228 6260 4715 Size: 32 1481 32 5101 Fragments arranged by size: 5101 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6646 PsiI TTA'TAA Cuts at: 4216 4216 Size: 6646 PspOMI G'GGCC_C Cuts at: 2156 2156 Size: 6646 PstI C_TGCA'G Cuts at: 830 2259 2682 2931 3408 3998 830 Size: 1429 423 249 477 590 3478 Fragments arranged by size: 3478 1429 590 477 423 249 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6646 PvuII CAG'CTG Cuts at: 2128 3622 3742 3769 2128 Size: 1494 120 27 5005 Fragments arranged by size: 5005 1494 120 27 SacI G_AGCT'C Cuts at: 2375 3342 3458 2375 Size: 967 116 5563 Fragments arranged by size: 5563 967 116 SacII CC_GC'GG Cuts at: 2301 2301 Size: 6646 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SapI GCTCTTCn'nnn_ Cuts at: 2225 3446 2225 Size: 1221 5425 SbfI CC_TGCA'GG Cuts at: 2682 3998 2682 Size: 1316 5330 ScaI AGT'ACT Cuts at: 4894 6606 4894 Size: 1712 4934 SexAI A'CCwGG_T Cuts at: 2460 2460 Size: 6646 SfcI C'TryA_G Cuts at: 826 1045 2255 2678 2927 3404 3830 3994 5129 Size: 219 1210 423 249 477 426 164 1135 Cuts at: 5129 5810 6001 826 Size: 681 191 1471 Fragments arranged by size: 1471 1210 1135 681 477 426 423 249 219 191 164 SfoI GGC'GCC Cuts at: 3229 3229 Size: 6646 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 3304 3973 1872 Size: 1432 669 4545 Fragments arranged by size: 4545 1432 669 SmlI C'TyrA_G Cuts at: 820 1017 1943 4750 5651 5913 820 Size: 197 926 2807 901 262 1553 Fragments arranged by size: 2807 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4437 493 Size: 3944 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 4004 1132 Size: 2872 3774 SspI AAT'ATT Cuts at: 5 52 4570 5 Size: 47 4518 2081 Fragments arranged by size: 4518 2081 47 StuI AGG'CCT Cuts at: 1517 2021 3749 1517 Size: 504 1728 4414 Fragments arranged by size: 4414 1728 504 StyI C'CwwG_G Cuts at: 513 1065 2427 2435 2452 3713 4085 513 Size: 552 1362 8 17 1261 372 3074 Fragments arranged by size: 3074 1362 1261 552 372 17 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2465 3051 4673 4690 4843 5028 5473 Size: 1255 885 586 1622 17 153 185 445 Cuts at: 5473 325 Size: 1498 Fragments arranged by size: 1622 1498 1255 885 586 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4892 6604 96 Size: 274 80 33 51 1443 2915 1712 138 Fragments arranged by size: 2915 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2027 2059 2448 2480 6606 6638 2027 Size: 32 389 32 4126 32 2035 Fragments arranged by size: 4126 2035 389 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3054 1316 Size: 1738 4908 XbaI T'CTAG_A Cuts at: 3982 3982 Size: 6646 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3100 3364 3383 3421 3511 3100 Size: 264 19 38 90 6235 Fragments arranged by size: 6235 264 90 38 19 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6646 XmaI C'CCGG_G Cuts at: 1870 3302 3971 1870 Size: 1432 669 4545 Fragments arranged by size: 4545 1432 669 XmnI GAAnn'nnTTC Cuts at: 4775 4775 Size: 6646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4454 276 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SanDI SfiI SrfI SwaI