(Circular) (Six-base) MAPSORT of: pFN21AB9909.seq Check: 94 from: 1 to: 5473 pFN21AB9909 5473 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3283 278 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 1937 Size: 879 4594 Acc65I G'GTAC_C Cuts at: 2794 2794 Size: 5473 AclI AA'CG_TT Cuts at: 1563 3600 3973 1563 Size: 2037 373 3063 Fragments arranged by size: 3063 2037 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3536 4920 5188 1632 Size: 1904 1384 268 1917 Fragments arranged by size: 1917 1904 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5276 AflIII A'CryG_T Cuts at: 1203 4372 5349 1203 Size: 3169 977 1327 Fragments arranged by size: 3169 1327 977 AgeI A'CCGG_T Cuts at: 4304 4304 Size: 5473 AhdI GACnn_n'nnGTC Cuts at: 4202 4202 Size: 5473 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5473 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5473 AlwNI CAG_nnn'CTG Cuts at: 2242 2461 2512 3216 4788 2242 Size: 219 51 704 1572 2927 Fragments arranged by size: 2927 1572 704 219 51 ApaLI G'TGCA_C Cuts at: 3530 4686 3530 Size: 1156 4317 ApoI r'AATT_y Cuts at: 1493 2782 3008 3160 5269 1493 Size: 1289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1289 226 152 AseI AT'TA_AT Cuts at: 160 1032 4027 160 Size: 872 2995 1606 Fragments arranged by size: 2995 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2155 2798 1870 Size: 73 130 82 643 4545 Fragments arranged by size: 4545 643 130 82 73 BamHI G'GATC_C Cuts at: 2730 2803 3170 2730 Size: 73 367 5033 Fragments arranged by size: 5033 367 73 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2177 2450 2486 2794 4249 Size: 325 217 627 390 273 36 308 1455 Cuts at: 4249 618 Size: 1842 Fragments arranged by size: 1842 1455 627 390 325 308 273 217 36 BanII G_rGCy'C Cuts at: 1391 2266 2289 2523 2792 1391 Size: 875 23 234 269 4072 Fragments arranged by size: 4072 875 269 234 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2584 2618 3664 3698 1903 Size: 34 647 34 1046 34 3678 Fragments arranged by size: 3678 1046 647 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3365 4575 3365 Size: 1210 4263 BclI T'GATC_A Cuts at: 1548 1548 Size: 5473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4084 136 Size: 107 122 71 631 605 2412 1525 Fragments arranged by size: 2412 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5468 5468 Size: 5473 BlpI GC'TnA_GC Cuts at: 2060 2375 2890 2060 Size: 315 515 4643 Fragments arranged by size: 4643 515 315 Bme1580I G_kGCm'C Cuts at: 1790 2100 2445 2491 3534 4690 1790 Size: 310 345 46 1043 1156 2573 Fragments arranged by size: 2573 1156 1043 345 310 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5473 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2449 3204 4162 Size: 416 53 83 399 24 1032 755 958 Cuts at: 4162 442 Size: 1753 Fragments arranged by size: 1753 1032 958 755 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4133 1239 Size: 2894 2579 Bpu10I CC'TnA_GC Cuts at: 2313 2313 Size: 5473 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3598 4463 4761 3598 Size: 865 298 4310 Fragments arranged by size: 4310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4136 882 Size: 617 2637 2219 Fragments arranged by size: 2637 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2722 3264 493 Size: 711 1518 542 2702 Fragments arranged by size: 2702 1518 711 542 BsaBI GATnn'nnATC Cuts at: 5467 5467 Size: 5473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 3280 3662 4297 Size: 53 83 186 936 503 1244 382 635 Cuts at: 4297 275 Size: 1451 Fragments arranged by size: 1451 1244 936 635 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3906 4304 4578 4725 5136 3906 Size: 398 274 147 411 4243 Fragments arranged by size: 4243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 1178 Size: 30 165 30 714 30 4504 Fragments arranged by size: 4504 714 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 1186 Size: 1168 4305 BseYI C'CCAG_C Cuts at: 2021 2192 2235 2292 2383 2472 2500 4676 5172 Size: 171 43 57 91 89 28 2176 496 Cuts at: 5172 2021 Size: 2322 Fragments arranged by size: 2322 2176 496 171 91 89 57 43 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4900 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2211 2923 3684 4712 1540 Size: 275 186 210 712 761 1028 2301 Fragments arranged by size: 2301 1028 761 712 275 210 186 BsiHKAI G_wGCw'C Cuts at: 2259 2523 3534 3619 4690 2259 Size: 264 1011 85 1071 3042 Fragments arranged by size: 3042 1071 1011 264 85 BsmI GAATG_Cn' Cuts at: 2984 3077 2984 Size: 93 5380 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2259 2266 2289 2445 2491 2523 Size: 399 310 159 7 23 156 46 32 Cuts at: 2523 2792 3534 3619 4690 1391 Size: 269 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 310 269 159 156 85 46 32 23 7 BspEI T'CCGG_A Cuts at: 5136 5136 Size: 5473 BspHI T'CATG_A Cuts at: 3362 5092 3362 Size: 1730 3743 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2828 844 Size: 1984 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2064 3360 1394 Size: 429 241 1296 3507 Fragments arranged by size: 3507 1296 429 241 BsrDI GCAATG_nn' Cuts at: 65 1248 3968 4142 65 Size: 1183 2720 174 1396 Fragments arranged by size: 2720 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2013 2550 2591 4117 4304 1700 Size: 110 203 537 41 1526 187 2869 Fragments arranged by size: 2869 1526 537 203 187 110 41 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5473 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5473 BssSI C'ACGA_G Cuts at: 2099 3533 4545 2099 Size: 1434 1012 3027 Fragments arranged by size: 3027 1434 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2221 2457 794 Size: 1427 236 3810 Fragments arranged by size: 3810 1427 236 BstBI TT'CG_AA Cuts at: 4277 4277 Size: 5473 BstEII G'GTnAC_C Cuts at: 1184 2536 1184 Size: 1352 4121 BstYI r'GATC_y Cuts at: 1972 2730 2803 3170 3555 3572 5024 5110 5122 Size: 758 73 367 385 17 1452 86 12 Cuts at: 5122 5468 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 758 385 367 346 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2446 2466 2532 513 Size: 552 939 442 20 66 3454 Fragments arranged by size: 3454 939 552 442 66 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4734 Fragments arranged by size: 4734 683 56 BtsI GCAGTG_nn' Cuts at: 2215 2989 3783 3803 2215 Size: 774 794 20 3885 Fragments arranged by size: 3885 794 774 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5473 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5438 DraI TTT'AAA Cuts at: 2777 2777 Size: 5473 DraIII CAC_nnn'GTG Cuts at: 2272 2457 2272 Size: 185 5288 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4480 809 Size: 507 3164 1802 Fragments arranged by size: 3164 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2463 2552 Size: 54 1425 50 275 18 378 255 89 Cuts at: 2552 2920 3809 5438 8 Size: 368 889 1629 43 Fragments arranged by size: 1629 1425 889 378 368 275 255 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2208 2920 1537 Size: 275 396 712 4090 Fragments arranged by size: 4090 712 396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3403 1353 Size: 2050 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4060 4434 4580 387 Size: 1064 138 2471 374 146 1280 Fragments arranged by size: 2471 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2521 2521 Size: 5473 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3536 4133 4920 5188 1239 Size: 393 1904 597 787 268 1524 Fragments arranged by size: 1904 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2509 2509 Size: 5473 EcoRI G'AATT_C Cuts at: 2782 3160 2782 Size: 378 5095 FspI TGC'GCA Cuts at: 3979 3979 Size: 5473 HaeII r_GCGC'y Cuts at: 1678 2242 4620 1678 Size: 564 2378 2531 Fragments arranged by size: 2531 2378 564 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4114 4146 4188 4220 1373 Size: 32 135 32 2542 32 42 32 2626 Fragments arranged by size: 2626 2542 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2817 3063 669 Size: 988 281 879 246 3079 Fragments arranged by size: 3079 988 879 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5473 HpaI GTT'AAC Cuts at: 3063 3063 Size: 5473 KpnI G_GTAC'C Cuts at: 2798 2798 Size: 5473 MfeI C'AATT_G Cuts at: 3072 3072 Size: 5473 MluI A'CGCG_T Cuts at: 5349 5349 Size: 5473 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4587 4771 1305 Size: 3282 184 2007 Fragments arranged by size: 3282 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2465 10 Size: 54 1425 343 633 3018 Fragments arranged by size: 3018 1425 633 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3431 3790 3949 518 Size: 648 2265 359 159 2042 Fragments arranged by size: 2265 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2374 2419 2889 3566 4714 4959 1606 Size: 768 45 470 677 1148 245 2120 Fragments arranged by size: 2120 1148 768 677 470 245 45 NaeI GCC'GGC Cuts at: 2552 2593 2552 Size: 41 5432 NcoI C'CATG_G Cuts at: 513 1065 2004 2466 513 Size: 552 939 462 3520 Fragments arranged by size: 3520 939 552 462 NdeI CA'TA_TG Cuts at: 387 387 Size: 5473 NgoMIV G'CCGG_C Cuts at: 2550 2591 2550 Size: 41 5432 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5473 NotI GC'GGCC_GC Cuts at: 2920 2920 Size: 5473 NspI r_CATG'y Cuts at: 1132 1229 2831 4376 1132 Size: 97 1602 1545 2229 Fragments arranged by size: 2229 1602 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5473 PciI A'CATG_T Cuts at: 4372 4372 Size: 5473 PflMI CCAn_nnn'nTGG Cuts at: 1108 2242 2461 2472 1108 Size: 1134 219 11 4109 Fragments arranged by size: 4109 1134 219 11 PmeI GTTT'AAAC Cuts at: 2777 2777 Size: 5473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3542 3574 5055 5087 3542 Size: 32 1481 32 3928 Fragments arranged by size: 3928 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2509 2509 Size: 5473 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5473 PsiI TTA'TAA Cuts at: 3043 3043 Size: 5473 PstI C_TGCA'G Cuts at: 830 2142 2517 2825 830 Size: 1312 375 308 3478 Fragments arranged by size: 3478 1312 375 308 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5473 PvuII CAG'CTG Cuts at: 2374 2419 2374 Size: 45 5428 SacI G_AGCT'C Cuts at: 2523 2523 Size: 5473 SalI G'TCGA_C Cuts at: 1936 2815 1936 Size: 879 4594 SbfI CC_TGCA'GG Cuts at: 2517 2825 2517 Size: 308 5165 ScaI AGT'ACT Cuts at: 3721 5433 3721 Size: 1712 3761 SfcI C'TryA_G Cuts at: 826 1045 2138 2513 2821 3956 4637 4828 826 Size: 219 1093 375 308 1135 681 191 1471 Fragments arranged by size: 1471 1135 1093 681 375 308 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5473 SmaI CCC'GGG Cuts at: 1872 2157 2800 1872 Size: 285 643 4545 Fragments arranged by size: 4545 643 285 SmlI C'TyrA_G Cuts at: 820 1017 1943 3577 4478 4740 820 Size: 197 926 1634 901 262 1553 Fragments arranged by size: 1634 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3264 493 Size: 2771 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5473 SphI G_CATG'C Cuts at: 1132 2831 1132 Size: 1699 3774 SspI AAT'ATT Cuts at: 5 52 3397 5 Size: 47 3345 2081 Fragments arranged by size: 3345 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5473 StyI C'CwwG_G Cuts at: 513 1065 2004 2466 2644 2760 2912 513 Size: 552 939 462 178 116 152 3074 Fragments arranged by size: 3074 939 552 462 178 152 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 2468 2474 3500 3517 3670 3855 Size: 1255 726 162 6 1026 17 153 185 Cuts at: 3855 4300 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1026 726 445 185 162 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3719 5431 96 Size: 274 80 33 51 1443 1742 1712 138 Fragments arranged by size: 1742 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5433 5465 5433 Size: 32 5441 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5473 XbaI T'CTAG_A Cuts at: 2809 2809 Size: 5473 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 5473 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5473 XmaI C'CCGG_G Cuts at: 1870 2155 2798 1870 Size: 285 643 4545 Fragments arranged by size: 4545 643 285 XmnI GAAnn'nnTTC Cuts at: 3602 3602 Size: 5473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3281 276 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI