(Circular) (Six-base) MAPSORT of: pFN21AB7973.seq Check: 3216 from: 1 to: 5347 pFN21AB7973 5347 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3157 278 Size: 53 83 186 936 1621 2468 Fragments arranged by size: 2468 1621 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2690 1937 Size: 753 4594 Acc65I G'GTAC_C Cuts at: 2668 2668 Size: 5347 AclI AA'CG_TT Cuts at: 1563 3474 3847 1563 Size: 1911 373 3063 Fragments arranged by size: 3063 1911 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 2545 3410 4794 5062 1632 Size: 560 353 865 1384 268 1917 Fragments arranged by size: 1917 1384 865 560 353 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5150 AflIII A'CryG_T Cuts at: 1203 2472 4246 5223 1203 Size: 1269 1774 977 1327 Fragments arranged by size: 1774 1327 1269 977 AgeI A'CCGG_T Cuts at: 4178 4178 Size: 5347 AhdI GACnn_n'nnGTC Cuts at: 4076 4076 Size: 5347 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5347 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2483 2515 2483 Size: 32 5315 AlwNI CAG_nnn'CTG Cuts at: 2545 2596 3090 4662 2545 Size: 51 494 1572 3230 Fragments arranged by size: 3230 1572 494 51 ApaLI G'TGCA_C Cuts at: 3404 4560 3404 Size: 1156 4191 ApoI r'AATT_y Cuts at: 1493 2656 2882 3034 5143 1493 Size: 1163 226 152 2109 1697 Fragments arranged by size: 2109 1697 1163 226 152 AseI AT'TA_AT Cuts at: 160 1032 3901 160 Size: 872 2869 1606 Fragments arranged by size: 2869 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5347 AvaI C'yCGr_G Cuts at: 1870 1943 2027 2672 1870 Size: 73 84 645 4545 Fragments arranged by size: 4545 645 84 73 BamHI G'GATC_C Cuts at: 2677 3044 2677 Size: 367 4980 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2668 4123 618 Size: 325 217 627 231 650 1455 1842 Fragments arranged by size: 1842 1455 650 627 325 231 217 BanII G_rGCy'C Cuts at: 1391 2666 1391 Size: 1275 4072 BbeI G_GCGC'C Cuts at: 2022 2022 Size: 5347 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2551 928 Size: 971 652 3724 Fragments arranged by size: 3724 971 652 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2257 2291 3538 3572 1903 Size: 34 320 34 1247 34 3678 Fragments arranged by size: 3678 1247 320 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3239 4449 3239 Size: 1210 4137 BclI T'GATC_A Cuts at: 1548 1548 Size: 5347 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3958 136 Size: 107 122 71 631 605 2286 1525 Fragments arranged by size: 2286 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5342 5342 Size: 5347 BlpI GC'TnA_GC Cuts at: 2163 2264 2764 2163 Size: 101 500 4746 Fragments arranged by size: 4746 500 101 Bme1580I G_kGCm'C Cuts at: 1790 3408 4564 1790 Size: 1618 1156 2573 Fragments arranged by size: 2573 1618 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5347 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3078 4036 442 Size: 416 53 83 399 24 1661 958 1753 Fragments arranged by size: 1753 1661 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5347 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4007 1239 Size: 2768 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3472 4337 4635 3472 Size: 865 298 4184 Fragments arranged by size: 4184 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2455 4010 882 Size: 617 956 1555 2219 Fragments arranged by size: 2219 1555 956 617 BsaAI yAC'GTr Cuts at: 493 1204 3138 493 Size: 711 1934 2702 Fragments arranged by size: 2702 1934 711 BsaBI GATnn'nnATC Cuts at: 5341 5341 Size: 5347 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 2181 2616 3154 Size: 53 83 186 936 486 162 435 538 Cuts at: 3154 3536 4171 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 538 486 435 382 186 162 83 53 BsaWI w'CCGG_w Cuts at: 3780 4178 4452 4599 5010 3780 Size: 398 274 147 411 4117 Fragments arranged by size: 4117 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2330 2360 1178 Size: 30 165 30 927 30 4165 Fragments arranged by size: 4165 927 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2188 2351 2354 2588 1186 Size: 1002 163 3 234 3945 Fragments arranged by size: 3945 1002 234 163 3 BseYI C'CCAG_C Cuts at: 4550 5046 4550 Size: 496 4851 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2606 1163 Size: 573 870 3904 Fragments arranged by size: 3904 870 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2797 3558 4586 1540 Size: 275 186 796 761 1028 2301 Fragments arranged by size: 2301 1028 796 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2580 3408 3493 4564 2580 Size: 828 85 1071 3363 Fragments arranged by size: 3363 1071 828 85 BsmI GAATG_Cn' Cuts at: 2858 2951 2858 Size: 93 5254 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2580 2666 3408 3493 4564 1391 Size: 399 790 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 790 742 399 86 85 BspEI T'CCGG_A Cuts at: 5010 5010 Size: 5347 BspHI T'CATG_A Cuts at: 2382 3236 4966 2382 Size: 854 1730 2763 Fragments arranged by size: 2763 1730 854 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2702 844 Size: 1858 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3234 1394 Size: 429 1411 3507 Fragments arranged by size: 3507 1411 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3842 4016 65 Size: 1183 2594 174 1396 Fragments arranged by size: 2594 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2136 3991 4178 1700 Size: 110 326 1855 187 2869 Fragments arranged by size: 2869 1855 326 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5347 BssHII G'CGCG_C Cuts at: 1925 2146 1925 Size: 221 5126 BssSI C'ACGA_G Cuts at: 3407 4419 3407 Size: 1012 4335 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5347 BstBI TT'CG_AA Cuts at: 4151 4151 Size: 5347 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5347 BstXI CCAn_nnnn'nTGG Cuts at: 2527 2527 Size: 5347 BstYI r'GATC_y Cuts at: 1972 2677 3044 3429 3446 4898 4984 4996 5342 Size: 705 367 385 17 1452 86 12 346 Cuts at: 5342 1972 Size: 1977 Fragments arranged by size: 1977 1452 705 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4795 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4608 Fragments arranged by size: 4608 683 56 BtsI GCAGTG_nn' Cuts at: 2863 3657 3677 2863 Size: 794 20 4533 Fragments arranged by size: 4533 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5312 DraI TTT'AAA Cuts at: 2651 2651 Size: 5347 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2039 4354 809 Size: 507 723 2315 1802 Fragments arranged by size: 2315 1802 723 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2529 2794 3683 Size: 54 1425 50 275 18 699 265 889 Cuts at: 3683 5312 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 699 275 265 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2794 1537 Size: 275 982 4090 Fragments arranged by size: 4090 982 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 3277 1353 Size: 812 1112 3423 Fragments arranged by size: 3423 1112 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3934 4308 4454 387 Size: 1064 138 2345 374 146 1280 Fragments arranged by size: 2345 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2545 3410 4007 4794 5062 1239 Size: 393 560 353 865 597 787 268 1524 Fragments arranged by size: 1524 865 787 597 560 393 353 268 EcoO109I rG'GnC_Cy Cuts at: 2032 2032 Size: 5347 EcoRI G'AATT_C Cuts at: 2656 3034 2656 Size: 378 4969 FspI TGC'GCA Cuts at: 3853 3853 Size: 5347 HaeII r_GCGC'y Cuts at: 1678 2022 4494 1678 Size: 344 2472 2531 Fragments arranged by size: 2531 2472 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2330 2362 2435 2467 3988 Size: 32 135 32 758 32 73 32 1521 Cuts at: 3988 4020 4062 4094 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1521 758 135 73 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2691 2937 669 Size: 988 281 753 246 3079 Fragments arranged by size: 3079 988 753 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5347 HpaI GTT'AAC Cuts at: 2937 2937 Size: 5347 KasI G'GCGC_C Cuts at: 2018 2018 Size: 5347 KpnI G_GTAC'C Cuts at: 2672 2672 Size: 5347 MfeI C'AATT_G Cuts at: 2627 2946 2627 Size: 319 5028 MluI A'CGCG_T Cuts at: 5223 5223 Size: 5347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4461 4645 1305 Size: 3156 184 2007 Fragments arranged by size: 3156 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2531 10 Size: 54 1425 343 699 2826 Fragments arranged by size: 2826 1425 699 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 2525 3305 3664 3823 518 Size: 648 1221 138 780 359 159 2042 Fragments arranged by size: 2042 1221 780 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2565 2763 3440 4588 4833 1606 Size: 959 198 677 1148 245 2120 Fragments arranged by size: 2120 1148 959 677 245 198 NarI GG'CG_CC Cuts at: 2019 2019 Size: 5347 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4795 NdeI CA'TA_TG Cuts at: 387 387 Size: 5347 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5347 NotI GC'GGCC_GC Cuts at: 2794 2794 Size: 5347 NspI r_CATG'y Cuts at: 1132 1229 2705 4250 1132 Size: 97 1476 1545 2229 Fragments arranged by size: 2229 1545 1476 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5347 PciI A'CATG_T Cuts at: 4246 4246 Size: 5347 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5347 PfoI T'CCnGG_A Cuts at: 2538 2538 Size: 5347 PmeI GTTT'AAAC Cuts at: 2651 2651 Size: 5347 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3416 3448 4929 4961 3416 Size: 32 1481 32 3802 Fragments arranged by size: 3802 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2032 Size: 5347 PshAI GACnn'nnGTC Cuts at: 1613 2216 1613 Size: 603 4744 PsiI TTA'TAA Cuts at: 2917 2917 Size: 5347 PstI C_TGCA'G Cuts at: 830 2699 830 Size: 1869 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5347 PvuII CAG'CTG Cuts at: 2565 2565 Size: 5347 SalI G'TCGA_C Cuts at: 1936 2689 1936 Size: 753 4594 SbfI CC_TGCA'GG Cuts at: 2699 2699 Size: 5347 ScaI AGT'ACT Cuts at: 3595 5307 3595 Size: 1712 3635 SexAI A'CCwGG_T Cuts at: 2445 2457 2445 Size: 12 5335 SfcI C'TryA_G Cuts at: 826 1045 2059 2695 3830 4511 4702 826 Size: 219 1014 636 1135 681 191 1471 Fragments arranged by size: 1471 1135 1014 681 636 219 191 SfoI GGC'GCC Cuts at: 2020 2020 Size: 5347 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5347 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5347 SmaI CCC'GGG Cuts at: 1872 2674 1872 Size: 802 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3451 4352 4614 820 Size: 197 926 1508 901 262 1553 Fragments arranged by size: 1553 1508 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3138 493 Size: 2645 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5347 SphI G_CATG'C Cuts at: 1132 2705 1132 Size: 1573 3774 SspI AAT'ATT Cuts at: 5 52 3271 5 Size: 47 3219 2081 Fragments arranged by size: 3219 2081 47 StuI AGG'CCT Cuts at: 1517 2114 1517 Size: 597 4750 StyI C'CwwG_G Cuts at: 513 1065 2036 2786 513 Size: 552 971 750 3074 Fragments arranged by size: 3074 971 750 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3374 3391 3544 3729 4174 325 Size: 1255 1794 17 153 185 445 1498 Fragments arranged by size: 1794 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3593 5305 96 Size: 274 80 33 51 1443 1616 1712 138 Fragments arranged by size: 1712 1616 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5307 5339 5307 Size: 32 5315 Tth111I GACn'n_nGTC Cuts at: 1316 2447 2459 1316 Size: 1131 12 4204 Fragments arranged by size: 4204 1131 12 XbaI T'CTAG_A Cuts at: 2683 2683 Size: 5347 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5347 XmaI C'CCGG_G Cuts at: 1870 2672 1870 Size: 802 4545 XmnI GAAnn'nnTTC Cuts at: 3476 3476 Size: 5347 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3155 276 Size: 53 83 186 936 1621 2468 Fragments arranged by size: 2468 1621 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI