Restriction map for pFN21AB7958
 (Circular) (Six-base) MAPSORT of: pFN21AB7958.seq  Check: 8332  from: 1  to: 5893



pFN21AB7958  5893 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3703     278 
   Size:         53      83     186     936    2167    2468

  Fragments arranged by size: 

                2468    2167     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3236    1937 
   Size:       1299    4594
 
Acc65I G'GTAC_C

Cuts at:   3214    3214 
   Size:       5893
 
AclI AA'CG_TT

Cuts at:   1563    4020    4393    1563 
   Size:       2457     373    3063

  Fragments arranged by size: 

                3063    2457     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3956    5340    5608    1632 
   Size:       2324    1384     268    1917

  Fragments arranged by size: 

                2324    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2993     820 
   Size:        197    1976    3720

  Fragments arranged by size: 

                3720    1976     197
 
AflIII A'CryG_T

Cuts at:   1203    2178    2252    4792    5769    1203 
   Size:        975      74    2540     977    1327

  Fragments arranged by size: 

                2540    1327     977     975      74
 
AgeI A'CCGG_T

Cuts at:   4724    4724 
   Size:       5893
 
AhdI GACnn_n'nnGTC

Cuts at:   2524    4622    2524 
   Size:       2098    3795
 
AleI CACnn'nnGTG

Cuts at:   1166    2133    2846    1166 
   Size:        967     713    4213

  Fragments arranged by size: 

                4213     967     713
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5893
 
AlwNI CAG_nnn'CTG

Cuts at:   3069    3113    3636    5208    3069 
   Size:         44     523    1572    3754

  Fragments arranged by size: 

                3754    1572     523      44
 
ApaLI G'TGCA_C

Cuts at:   3950    5106    3950 
   Size:       1156    4737
 
ApoI r'AATT_y

Cuts at:   1493    2891    2921    3037    3202    3428    3580    5689    1493 
   Size:       1398      30     116     165     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1398     226     165     152     116      30
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4447     160 
   Size:        872    3415    1606

  Fragments arranged by size: 

                3415    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5893
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3218    1870 
   Size:         73    1275    4545

  Fragments arranged by size: 

                4545    1275      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2309    2342    2309 
   Size:         33    5860
 
BamHI G'GATC_C

Cuts at:   2531    3223    3590    2531 
   Size:        692     367    4834

  Fragments arranged by size: 

                4834     692     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3214    4669     618 
   Size:        325     217     627    1427    1455    1842

  Fragments arranged by size: 

                1842    1455    1427     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3212    1391 
   Size:       1821    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4922
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4084    4118    1903 
   Size:         34      50      34    2063      34    3678

  Fragments arranged by size: 

                3678    2063      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2337    2380    3785    4995    2337 
   Size:         43    1405    1210    3235

  Fragments arranged by size: 

                3235    1405    1210      43
 
BclI T'GATC_A

Cuts at:   1548    2683    1548 
   Size:       1135    4758
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4504     136 
   Size:        107     122      71     631     605    2832    1525

  Fragments arranged by size: 

                2832    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5888    5888 
   Size:       5893
 
BlpI GC'TnA_GC

Cuts at:   3310    3310 
   Size:       5893
 
Bme1580I G_kGCm'C

Cuts at:   1790    3954    5110    1790 
   Size:       2164    1156    2573

  Fragments arranged by size: 

                2573    2164    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5893
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3624    4582     442 
   Size:        416      53      83     399      24    2207     958    1753

  Fragments arranged by size: 

                2207    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5893
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2215    2439    3190    4553    1239 
   Size:        976     224     751    1363    2579

  Fragments arranged by size: 

                2579    1363     976     751     224
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2243    4018    4883    5181    2243 
   Size:       1775     865     298    2955

  Fragments arranged by size: 

                2955    1775     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2592    4556     882 
   Size:        617    1093    1964    2219

  Fragments arranged by size: 

                2219    1964    1093     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3684     493 
   Size:        711    2480    2702

  Fragments arranged by size: 

                2702    2480     711
 
BsaBI GATnn'nnATC

Cuts at:   5887    5887 
   Size:       5893
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3700    4082    4717     275 
   Size:         53      83     186     936    2167     382     635    1451

  Fragments arranged by size: 

                2167    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4326    4724    4998    5145    5556    4326 
   Size:        398     274     147     411    4663

  Fragments arranged by size: 

                4663     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2766    2796    3163    3193    1178 
   Size:         30     165      30    1363      30     367      30    3878

  Fragments arranged by size: 

                3878    1363     367     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2774    1186 
   Size:       1588    4305
 
BseYI C'CCAG_C

Cuts at:   2008    5096    5592    2008 
   Size:       3088     496    2309

  Fragments arranged by size: 

                3088    2309     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5320
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3343    4104    5132    1540 
   Size:        275     186    1342     761    1028    2301

  Fragments arranged by size: 

                2301    1342    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3954    4039    5110    3954 
   Size:         85    1071    4737

  Fragments arranged by size: 

                4737    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3404    3497    3404 
   Size:         93    5800
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3212    3954    4039    5110    1391 
   Size:        399    1422     742      85    1071    2174

  Fragments arranged by size: 

                2174    1422    1071     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5556    5556 
   Size:       5893
 
BspHI T'CATG_A

Cuts at:   2332    3782    5512    2332 
   Size:       1450    1730    2713

  Fragments arranged by size: 

                2713    1730    1450
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3248     844 
   Size:       2404    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3780    1394 
   Size:        429    1957    3507

  Fragments arranged by size: 

                3507    1957     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2949    4388    4562      65 
   Size:       1183    1701    1439     174    1396

  Fragments arranged by size: 

                1701    1439    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4537    4724    1700 
   Size:        110    2727     187    2869

  Fragments arranged by size: 

                2869    2727     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2317      96 
   Size:       2221    3672
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5893
 
BssSI C'ACGA_G

Cuts at:   3953    4965    3953 
   Size:       1012    4881
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2729    3069     794 
   Size:       1935     340    3618

  Fragments arranged by size: 

                3618    1935     340
 
BstBI TT'CG_AA

Cuts at:   4697    4697 
   Size:       5893
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5893
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2135    2135 
   Size:       5893
 
BstYI r'GATC_y

Cuts at:   1972    2531    3223    3590    3975    3992    5444    5530    5542 
   Size:        559     692     367     385      17    1452      86      12

Cuts at:   5542    5888    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     692     559     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2128     513 
   Size:        552    1063    4278

  Fragments arranged by size: 

                4278    1063     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5154

  Fragments arranged by size: 

                5154     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2268    2727    3184    3409    4203    4223    2268 
   Size:        459     457     225     794      20    3938

  Fragments arranged by size: 

                3938     794     459     457     225      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5893
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5858
 
DraI TTT'AAA

Cuts at:   3197    3197 
   Size:       5893
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4900     809 
   Size:        507    3584    1802

  Fragments arranged by size: 

                3584    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3340    4229    5858 
   Size:         54    1425      50     275      18    1510     889    1629

Cuts at:   5858       8 
   Size:         43

  Fragments arranged by size: 

                1629    1510    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3340    1537 
   Size:        275    1528    4090

  Fragments arranged by size: 

                4090    1528     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2055    2650    3823    1353 
   Size:        702     595    1173    3423

  Fragments arranged by size: 

                3423    1173     702     595
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4480    4854    5000     387 
   Size:       1064     138    2891     374     146    1280

  Fragments arranged by size: 

                2891    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2215    2439    3190    3956    4553    5340    5608 
   Size:        393     583     224     751     766     597     787     268

Cuts at:   5608    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     787     766     751     597     583     393     268
                 224
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2166    2166 
   Size:       5893
 
EcoO109I rG'GnC_Cy

Cuts at:   2044    2498    2044 
   Size:        454    5439
 
EcoRI G'AATT_C

Cuts at:   3202    3580    3202 
   Size:        378    5515
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2528    2528 
   Size:       5893
 
FspI TGC'GCA

Cuts at:   4399    4399 
   Size:       5893
 
HaeII r_GCGC'y

Cuts at:   1678    5040    1678 
   Size:       3362    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2510    2542    2766    2798    4534 
   Size:         32     135      32     938      32     224      32    1736

Cuts at:   4534    4566    4608    4640    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1736     938     224     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2073    3016    3237    3483     669 
   Size:        988     281     135     943     221     246    3079

  Fragments arranged by size: 

                3079     988     943     281     246     221     135
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5893
 
HpaI GTT'AAC

Cuts at:   3483    3483 
   Size:       5893
 
KpnI G_GTAC'C

Cuts at:   3218    3218 
   Size:       5893
 
MfeI C'AATT_G

Cuts at:   3492    3492 
   Size:       5893
 
MluI A'CGCG_T

Cuts at:   5769    5769 
   Size:       5893
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5007    5191    1305 
   Size:       3702     184    2007

  Fragments arranged by size: 

                3702    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4071

  Fragments arranged by size: 

                4071    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2133    2289    2342    2665    2846    3851    4210 
   Size:        648     967     156      53     323     181    1005     359

Cuts at:   4210    4369     518 
   Size:        159    2042

  Fragments arranged by size: 

                2042    1005     967     648     359     323     181     159
                 156      53
 
MspA1I CmG'CkG

Cuts at:   1606    3309    3986    5134    5379    1606 
   Size:       1703     677    1148     245    2120

  Fragments arranged by size: 

                2120    1703    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5341
 
NdeI CA'TA_TG

Cuts at:    387    2448    2938     387 
   Size:       2061     490    3342

  Fragments arranged by size: 

                3342    2061     490
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5893
 
NotI GC'GGCC_GC

Cuts at:   3340    3340 
   Size:       5893
 
NspI r_CATG'y

Cuts at:   1132    1229    2182    2256    2347    3251    4796    1132 
   Size:         97     953      74      91     904    1545    2229

  Fragments arranged by size: 

                2229    1545     953     904      97      91      74
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5893
 
PciI A'CATG_T

Cuts at:   2178    2252    4792    2178 
   Size:         74    2540    3279

  Fragments arranged by size: 

                3279    2540      74
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5893
 
PfoI T'CCnGG_A

Cuts at:   3042    3042 
   Size:       5893
 
PmeI GTTT'AAAC

Cuts at:   3197    3197 
   Size:       5893
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3962    3994    5475    5507    3962 
   Size:         32    1481      32    4348

  Fragments arranged by size: 

                4348    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2044    2498    2044 
   Size:        454    5439
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5893
 
PsiI TTA'TAA

Cuts at:   3006    3463    3006 
   Size:        457    5436
 
PstI C_TGCA'G

Cuts at:    830    2410    2915    3245     830 
   Size:       1580     505     330    3478

  Fragments arranged by size: 

                3478    1580     505     330
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5893
 
SalI G'TCGA_C

Cuts at:   1936    3235    1936 
   Size:       1299    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2650    2650 
   Size:       5893
 
SbfI CC_TGCA'GG

Cuts at:   3245    3245 
   Size:       5893
 
ScaI AGT'ACT

Cuts at:   4141    5853    4141 
   Size:       1712    4181
 
SfcI C'TryA_G

Cuts at:    826    1045    2406    2549    2911    3241    4376    5057    5248 
   Size:        219    1361     143     362     330    1135     681     191

Cuts at:   5248     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1361    1135     681     362     330     219     191
                 143
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5893
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5893
 
SmaI CCC'GGG

Cuts at:   1872    3220    1872 
   Size:       1348    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2222    2993    3997    4898    5160     820 
   Size:        197     926     279     771    1004     901     262    1553

  Fragments arranged by size: 

                1553    1004     926     901     771     279     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3684     493 
   Size:       3191    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5893
 
SphI G_CATG'C

Cuts at:   1132    3251    1132 
   Size:       2119    3774
 
SspI AAT'ATT

Cuts at:      5      52    2571    3817       5 
   Size:         47    2519    1246    2081

  Fragments arranged by size: 

                2519    2081    1246      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5893
 
StyI C'CwwG_G

Cuts at:    513    1065    3332     513 
   Size:        552    2267    3074

  Fragments arranged by size: 

                3074    2267     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3920    3937    4090    4275    4720     325 
   Size:       1255    2340      17     153     185     445    1498

  Fragments arranged by size: 

                2340    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2209    2317    2506 
   Size:        274      80      33      51    1443     232     108     189

Cuts at:   2506    4139    5851      96 
   Size:       1633    1712     138

  Fragments arranged by size: 

                1712    1633    1443     274     232     189     138     108
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3163    3195    5853    5885    3163 
   Size:         32    2658      32    3171

  Fragments arranged by size: 

                3171    2658      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5893
 
XbaI T'CTAG_A

Cuts at:   3229    3229 
   Size:       5893
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2473    2473 
   Size:       5893
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5893
 
XmaI C'CCGG_G

Cuts at:   1870    3218    1870 
   Size:       1348    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3108    4022    3108 
   Size:        914    4979
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3701     276 
   Size:         53      83     186     936    2167    2468

  Fragments arranged by size: 

                2468    2167     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI     SalI     SapI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    PvuII
    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SfoI     SrfI
     SwaI
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