(Circular) (Six-base) MAPSORT of: pFN21AB7958.seq Check: 8332 from: 1 to: 5893 pFN21AB7958 5893 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3236 1937 Size: 1299 4594 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3956 5340 5608 1632 Size: 2324 1384 268 1917 Fragments arranged by size: 2324 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2993 820 Size: 197 1976 3720 Fragments arranged by size: 3720 1976 197 AflIII A'CryG_T Cuts at: 1203 2178 2252 4792 5769 1203 Size: 975 74 2540 977 1327 Fragments arranged by size: 2540 1327 977 975 74 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 2524 4622 2524 Size: 2098 3795 AleI CACnn'nnGTG Cuts at: 1166 2133 2846 1166 Size: 967 713 4213 Fragments arranged by size: 4213 967 713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5893 AlwNI CAG_nnn'CTG Cuts at: 3069 3113 3636 5208 3069 Size: 44 523 1572 3754 Fragments arranged by size: 3754 1572 523 44 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 2891 2921 3037 3202 3428 3580 5689 1493 Size: 1398 30 116 165 226 152 2109 1697 Fragments arranged by size: 2109 1697 1398 226 165 152 116 30 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 3218 1870 Size: 73 1275 4545 Fragments arranged by size: 4545 1275 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2309 2342 2309 Size: 33 5860 BamHI G'GATC_C Cuts at: 2531 3223 3590 2531 Size: 692 367 4834 Fragments arranged by size: 4834 692 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3214 4669 618 Size: 325 217 627 1427 1455 1842 Fragments arranged by size: 1842 1455 1427 627 325 217 BanII G_rGCy'C Cuts at: 1391 3212 1391 Size: 1821 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4922 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4084 4118 1903 Size: 34 50 34 2063 34 3678 Fragments arranged by size: 3678 2063 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2337 2380 3785 4995 2337 Size: 43 1405 1210 3235 Fragments arranged by size: 3235 1405 1210 43 BclI T'GATC_A Cuts at: 1548 2683 1548 Size: 1135 4758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4504 136 Size: 107 122 71 631 605 2832 1525 Fragments arranged by size: 2832 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 3310 3310 Size: 5893 Bme1580I G_kGCm'C Cuts at: 1790 3954 5110 1790 Size: 2164 1156 2573 Fragments arranged by size: 2573 2164 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5893 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3624 4582 442 Size: 416 53 83 399 24 2207 958 1753 Fragments arranged by size: 2207 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2215 2439 3190 4553 1239 Size: 976 224 751 1363 2579 Fragments arranged by size: 2579 1363 976 751 224 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2243 4018 4883 5181 2243 Size: 1775 865 298 2955 Fragments arranged by size: 2955 1775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2592 4556 882 Size: 617 1093 1964 2219 Fragments arranged by size: 2219 1964 1093 617 BsaAI yAC'GTr Cuts at: 493 1204 3684 493 Size: 711 2480 2702 Fragments arranged by size: 2702 2480 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3700 4082 4717 275 Size: 53 83 186 936 2167 382 635 1451 Fragments arranged by size: 2167 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4326 4724 4998 5145 5556 4326 Size: 398 274 147 411 4663 Fragments arranged by size: 4663 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2766 2796 3163 3193 1178 Size: 30 165 30 1363 30 367 30 3878 Fragments arranged by size: 3878 1363 367 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2774 1186 Size: 1588 4305 BseYI C'CCAG_C Cuts at: 2008 5096 5592 2008 Size: 3088 496 2309 Fragments arranged by size: 3088 2309 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3954 4039 5110 3954 Size: 85 1071 4737 Fragments arranged by size: 4737 1071 85 BsmI GAATG_Cn' Cuts at: 3404 3497 3404 Size: 93 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3212 3954 4039 5110 1391 Size: 399 1422 742 85 1071 2174 Fragments arranged by size: 2174 1422 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5556 5556 Size: 5893 BspHI T'CATG_A Cuts at: 2332 3782 5512 2332 Size: 1450 1730 2713 Fragments arranged by size: 2713 1730 1450 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3248 844 Size: 2404 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2949 4388 4562 65 Size: 1183 1701 1439 174 1396 Fragments arranged by size: 1701 1439 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4537 4724 1700 Size: 110 2727 187 2869 Fragments arranged by size: 2869 2727 187 110 BsrGI T'GTAC_A Cuts at: 96 2317 96 Size: 2221 3672 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 2729 3069 794 Size: 1935 340 3618 Fragments arranged by size: 3618 1935 340 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstXI CCAn_nnnn'nTGG Cuts at: 2135 2135 Size: 5893 BstYI r'GATC_y Cuts at: 1972 2531 3223 3590 3975 3992 5444 5530 5542 Size: 559 692 367 385 17 1452 86 12 Cuts at: 5542 5888 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 692 559 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2128 513 Size: 552 1063 4278 Fragments arranged by size: 4278 1063 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5154 Fragments arranged by size: 5154 683 56 BtsI GCAGTG_nn' Cuts at: 2268 2727 3184 3409 4203 4223 2268 Size: 459 457 225 794 20 3938 Fragments arranged by size: 3938 794 459 457 225 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3197 3197 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858 Size: 54 1425 50 275 18 1510 889 1629 Cuts at: 5858 8 Size: 43 Fragments arranged by size: 1629 1510 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 2650 3823 1353 Size: 702 595 1173 3423 Fragments arranged by size: 3423 1173 702 595 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4480 4854 5000 387 Size: 1064 138 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2215 2439 3190 3956 4553 5340 5608 Size: 393 583 224 751 766 597 787 268 Cuts at: 5608 1239 Size: 1524 Fragments arranged by size: 1524 787 766 751 597 583 393 268 224 EcoNI CCTnn'n_nnAGG Cuts at: 2166 2166 Size: 5893 EcoO109I rG'GnC_Cy Cuts at: 2044 2498 2044 Size: 454 5439 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2528 2528 Size: 5893 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 5040 1678 Size: 3362 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2510 2542 2766 2798 4534 Size: 32 135 32 938 32 224 32 1736 Cuts at: 4534 4566 4608 4640 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1736 938 224 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2073 3016 3237 3483 669 Size: 988 281 135 943 221 246 3079 Fragments arranged by size: 3079 988 943 281 246 221 135 HindIII A'AGCT_T Cuts at: 748 748 Size: 5893 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5007 5191 1305 Size: 3702 184 2007 Fragments arranged by size: 3702 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4071 Fragments arranged by size: 4071 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2133 2289 2342 2665 2846 3851 4210 Size: 648 967 156 53 323 181 1005 359 Cuts at: 4210 4369 518 Size: 159 2042 Fragments arranged by size: 2042 1005 967 648 359 323 181 159 156 53 MspA1I CmG'CkG Cuts at: 1606 3309 3986 5134 5379 1606 Size: 1703 677 1148 245 2120 Fragments arranged by size: 2120 1703 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5341 NdeI CA'TA_TG Cuts at: 387 2448 2938 387 Size: 2061 490 3342 Fragments arranged by size: 3342 2061 490 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 2182 2256 2347 3251 4796 1132 Size: 97 953 74 91 904 1545 2229 Fragments arranged by size: 2229 1545 953 904 97 91 74 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5893 PciI A'CATG_T Cuts at: 2178 2252 4792 2178 Size: 74 2540 3279 Fragments arranged by size: 3279 2540 74 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5893 PfoI T'CCnGG_A Cuts at: 3042 3042 Size: 5893 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2044 2498 2044 Size: 454 5439 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 3006 3463 3006 Size: 457 5436 PstI C_TGCA'G Cuts at: 830 2410 2915 3245 830 Size: 1580 505 330 3478 Fragments arranged by size: 3478 1580 505 330 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SapI GCTCTTCn'nnn_ Cuts at: 2650 2650 Size: 5893 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SfcI C'TryA_G Cuts at: 826 1045 2406 2549 2911 3241 4376 5057 5248 Size: 219 1361 143 362 330 1135 681 191 Cuts at: 5248 826 Size: 1471 Fragments arranged by size: 1471 1361 1135 681 362 330 219 191 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 3220 1872 Size: 1348 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 2993 3997 4898 5160 820 Size: 197 926 279 771 1004 901 262 1553 Fragments arranged by size: 1553 1004 926 901 771 279 262 197 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3251 1132 Size: 2119 3774 SspI AAT'ATT Cuts at: 5 52 2571 3817 5 Size: 47 2519 1246 2081 Fragments arranged by size: 2519 2081 1246 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5893 StyI C'CwwG_G Cuts at: 513 1065 3332 513 Size: 552 2267 3074 Fragments arranged by size: 3074 2267 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3920 3937 4090 4275 4720 325 Size: 1255 2340 17 153 185 445 1498 Fragments arranged by size: 2340 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2209 2317 2506 Size: 274 80 33 51 1443 232 108 189 Cuts at: 2506 4139 5851 96 Size: 1633 1712 138 Fragments arranged by size: 1712 1633 1443 274 232 189 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3163 3195 5853 5885 3163 Size: 32 2658 32 3171 Fragments arranged by size: 3171 2658 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2473 2473 Size: 5893 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 3218 1870 Size: 1348 4545 XmnI GAAnn'nnTTC Cuts at: 3108 4022 3108 Size: 914 4979 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI