(Circular) (Six-base) MAPSORT of: pFN21AB7950.seq Check: 1087 from: 1 to: 5887 pFN21AB7950 5887 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3230 1937 Size: 1293 4594 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2763 3950 5334 5602 1632 Size: 1131 1187 1384 268 1917 Fragments arranged by size: 1917 1384 1187 1131 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 4786 5763 1203 Size: 3583 977 1327 Fragments arranged by size: 3583 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2751 1184 Size: 1567 4320 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2339 2371 2339 Size: 32 5855 AlwNI CAG_nnn'CTG Cuts at: 2635 3630 5202 2635 Size: 995 1572 3320 Fragments arranged by size: 3320 1572 995 ApaLI G'TGCA_C Cuts at: 2296 3944 5100 2296 Size: 1648 1156 3083 Fragments arranged by size: 3083 1648 1156 ApoI r'AATT_y Cuts at: 1493 2189 3196 3422 3574 5683 1493 Size: 696 1007 226 152 2109 1697 Fragments arranged by size: 2109 1697 1007 696 226 152 AscI GG'CGCG_CC Cuts at: 2442 2442 Size: 5887 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 2035 2261 2319 2397 2448 3212 1870 Size: 73 92 226 58 78 51 764 4545 Fragments arranged by size: 4545 764 226 92 78 73 58 51 BamHI G'GATC_C Cuts at: 2683 3217 3584 2683 Size: 534 367 4986 Fragments arranged by size: 4986 534 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2164 2200 2515 3016 3208 Size: 325 217 627 377 36 315 501 192 Cuts at: 3208 4663 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 501 377 325 315 217 192 36 BanII G_rGCy'C Cuts at: 1391 2579 3206 1391 Size: 1188 627 4072 Fragments arranged by size: 4072 1188 627 BbeI G_GCGC'C Cuts at: 2168 2519 2168 Size: 351 5536 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4916 BbvCI CC'TCA_GC Cuts at: 3005 3005 Size: 5887 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4078 4112 1903 Size: 34 2141 34 3678 Fragments arranged by size: 3678 2141 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 1548 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5882 5882 Size: 5887 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 2300 3021 3948 5104 1790 Size: 510 721 927 1156 2573 Fragments arranged by size: 2573 1156 927 721 510 BmgBI CAC'GTC Cuts at: 1318 2217 1318 Size: 899 4988 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2884 3618 4576 Size: 416 53 83 399 24 1467 734 958 Cuts at: 4576 442 Size: 1753 Fragments arranged by size: 1753 1467 958 734 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2649 2649 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2098 2429 2736 2828 2981 4547 1239 Size: 806 53 331 307 92 153 1566 2579 Fragments arranged by size: 2579 1566 806 331 307 153 92 53 Bpu10I CC'TnA_GC Cuts at: 3005 3062 3005 Size: 57 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4012 4877 5175 4012 Size: 865 298 4724 Fragments arranged by size: 4724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4550 882 Size: 617 3051 2219 Fragments arranged by size: 3051 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3678 493 Size: 711 2474 2702 Fragments arranged by size: 2702 2474 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2165 2468 2516 3694 Size: 53 83 186 936 632 303 48 1178 Cuts at: 3694 4076 4711 275 Size: 382 635 1451 Fragments arranged by size: 1451 1178 936 635 632 382 303 186 83 53 48 BsaWI w'CCGG_w Cuts at: 2300 4320 4718 4992 5139 5550 2300 Size: 2020 398 274 147 411 2637 Fragments arranged by size: 2637 2020 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2339 2369 1178 Size: 30 165 30 936 30 4696 Fragments arranged by size: 4696 936 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2855 1186 Size: 1669 4218 BseYI C'CCAG_C Cuts at: 2168 2337 2492 2536 5090 5586 2168 Size: 169 155 44 2554 496 2469 Fragments arranged by size: 2554 2469 496 169 155 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2470 1163 Size: 573 734 4580 Fragments arranged by size: 4580 734 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2428 3337 4098 5126 1540 Size: 275 186 427 909 761 1028 2301 Fragments arranged by size: 2301 1028 909 761 427 275 186 BsiHKAI G_wGCw'C Cuts at: 2300 3948 4033 5104 2300 Size: 1648 85 1071 3083 Fragments arranged by size: 3083 1648 1071 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 BsmBI CGTCTCn'nnnn_ Cuts at: 2298 2298 Size: 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2300 2579 3021 3206 3948 4033 5104 Size: 399 510 279 442 185 742 85 1071 Cuts at: 5104 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 510 442 399 279 185 85 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 3776 5506 3776 Size: 1730 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2991 3242 844 Size: 2147 251 3489 Fragments arranged by size: 3489 2147 251 BsrBI CCG'CTC Cuts at: 1394 1823 2059 2090 2157 2385 3774 1394 Size: 429 236 31 67 228 1389 3507 Fragments arranged by size: 3507 1389 429 236 228 67 31 BsrDI GCAATG_nn' Cuts at: 65 1248 4382 4556 65 Size: 1183 3134 174 1396 Fragments arranged by size: 3134 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 2051 2427 2504 2650 3141 4531 Size: 110 230 11 376 77 146 491 1390 Cuts at: 4531 4718 1700 Size: 187 2869 Fragments arranged by size: 2869 1390 491 376 230 187 146 110 77 11 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 2442 1925 Size: 517 5370 BssSI C'ACGA_G Cuts at: 3947 4959 3947 Size: 1012 4875 BstAPI GCAn_nnn'nTGC Cuts at: 794 3154 794 Size: 2360 3527 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 2653 1184 Size: 1469 4418 BstYI r'GATC_y Cuts at: 1972 2683 3217 3584 3969 3986 5438 5524 5536 Size: 711 534 367 385 17 1452 86 12 Cuts at: 5536 5882 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 711 534 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2160 2282 513 Size: 552 1095 122 4118 Fragments arranged by size: 4118 1095 552 122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2190 487 Size: 683 56 964 4184 Fragments arranged by size: 4184 964 683 56 BtsI GCAGTG_nn' Cuts at: 3403 4197 4217 3403 Size: 794 20 5073 Fragments arranged by size: 5073 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4894 809 Size: 507 3578 1802 Fragments arranged by size: 3578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3027 3334 4223 Size: 54 1425 50 275 18 1197 307 889 Cuts at: 4223 5852 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1197 889 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2618 2766 2793 2844 3101 3817 1353 Size: 1265 148 27 51 257 716 3423 Fragments arranged by size: 3423 1265 716 257 148 51 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2461 2989 4474 4848 4994 387 Size: 1064 138 872 528 1485 374 146 1280 Fragments arranged by size: 1485 1280 1064 872 528 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2098 2429 2736 2763 2828 2981 Size: 393 413 53 331 307 27 65 153 Cuts at: 2981 3950 4547 5334 5602 1239 Size: 969 597 787 268 1524 Fragments arranged by size: 1524 969 787 597 413 393 331 307 268 153 65 53 27 EcoNI CCTnn'n_nnAGG Cuts at: 3065 3065 Size: 5887 EcoO109I rG'GnC_Cy Cuts at: 2237 2251 2789 3043 2237 Size: 14 538 254 5081 Fragments arranged by size: 5081 538 254 14 EcoRI G'AATT_C Cuts at: 3196 3574 3196 Size: 378 5509 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 2168 2519 3005 5034 1678 Size: 490 351 486 2029 2531 Fragments arranged by size: 2531 2029 490 486 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2845 2877 2932 Size: 32 135 32 518 32 723 32 55 Cuts at: 2932 2964 4528 4560 4602 4634 1373 Size: 32 1564 32 42 32 2626 Fragments arranged by size: 2626 1564 723 518 135 55 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3231 3477 669 Size: 988 281 1293 246 3079 Fragments arranged by size: 3079 1293 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KasI G'GCGC_C Cuts at: 2164 2515 2164 Size: 351 5536 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5001 5185 1305 Size: 3696 184 2007 Fragments arranged by size: 3696 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3029 10 Size: 54 1425 343 1197 2868 Fragments arranged by size: 2868 1425 1197 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2663 2733 3845 4204 4363 518 Size: 648 843 654 70 1112 359 159 2042 Fragments arranged by size: 2042 1112 843 654 648 359 159 70 MspA1I CmG'CkG Cuts at: 1606 2536 3033 3303 3980 5128 5373 1606 Size: 930 497 270 677 1148 245 2120 Fragments arranged by size: 2120 1148 930 677 497 270 245 NaeI GCC'GGC Cuts at: 2042 2506 3143 2042 Size: 464 637 4786 Fragments arranged by size: 4786 637 464 NarI GG'CG_CC Cuts at: 2165 2516 2165 Size: 351 5536 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5335 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NgoMIV G'CCGG_C Cuts at: 2040 2504 3141 2040 Size: 464 637 4786 Fragments arranged by size: 4786 637 464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NruI TCG'CGA Cuts at: 2992 2992 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 2380 3245 4790 1132 Size: 97 1151 865 1545 2229 Fragments arranged by size: 2229 1545 1151 865 97 PasI CC'CwG_GG Cuts at: 1348 2226 1348 Size: 878 5009 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5887 PfoI T'CCnGG_A Cuts at: 2958 2958 Size: 5887 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2339 2371 3956 3988 5469 5501 2339 Size: 32 1585 32 1481 32 2725 Fragments arranged by size: 2725 1585 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2237 2251 2789 2237 Size: 14 538 5335 Fragments arranged by size: 5335 538 14 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PstI C_TGCA'G Cuts at: 830 2824 3239 830 Size: 1994 415 3478 Fragments arranged by size: 3478 1994 415 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 PvuII CAG'CTG Cuts at: 3033 3033 Size: 5887 RsrII CG'GwC_CG Cuts at: 2054 2054 Size: 5887 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SanDI GG'GwC_CC Cuts at: 2237 2251 2789 2237 Size: 14 538 5335 Fragments arranged by size: 5335 538 14 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SexAI A'CCwGG_T Cuts at: 3012 3012 Size: 5887 SfcI C'TryA_G Cuts at: 826 1045 2820 2871 3117 3235 4370 5051 5242 Size: 219 1775 51 246 118 1135 681 191 Cuts at: 5242 826 Size: 1471 Fragments arranged by size: 1775 1471 1135 681 246 219 191 118 51 SfoI GGC'GCC Cuts at: 2166 2517 2166 Size: 351 5536 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 2263 2321 2399 2450 3214 1872 Size: 391 58 78 51 764 4545 Fragments arranged by size: 4545 764 391 78 58 51 SmlI C'TyrA_G Cuts at: 820 1017 1943 3991 4892 5154 820 Size: 197 926 2048 901 262 1553 Fragments arranged by size: 2048 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 2558 3071 1517 Size: 1041 513 4333 Fragments arranged by size: 4333 1041 513 StyI C'CwwG_G Cuts at: 513 1065 2463 3039 3072 3326 513 Size: 552 1398 576 33 254 3074 Fragments arranged by size: 3074 1398 576 552 254 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3914 3931 4084 4269 4714 325 Size: 1255 2334 17 153 185 445 1498 Fragments arranged by size: 2334 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4133 5845 96 Size: 274 80 33 51 1443 2156 1712 138 Fragments arranged by size: 2156 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5887 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 2261 2319 2397 2448 3212 1870 Size: 391 58 78 51 764 4545 Fragments arranged by size: 4545 764 391 78 58 51 XmnI GAAnn'nnTTC Cuts at: 2189 2699 2802 4016 2189 Size: 510 103 1214 4060 Fragments arranged by size: 4060 1214 510 103 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NsiI PacI PmlI PspOMI PspXI PsrI SacI SacII SapI SfiI SrfI SwaI XcmI