Restriction map for
pFN21AB7940
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaHI BseRI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MluI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 367 TCCGGA T^CCGGA
AciI 8 CCGC CCGC(-3/-1)
Alw44I 511 GTGCAC G^TGCAC
AlwI 201 GATCC GGATC(4/5)
ApaLI 511 GTGCAC G^TGCAC
AspI 162 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 419 CCCGGG C^[CT]CG[GA]G
BalI 254 TGGCCA TGG^CCA
BglII 327 AGATCT A^GATCT
BpmI 170 CTCCAG CTGGAG(16/14)
BsaAI 558 CACGTA [CT]AC^GT[GA]
BsaBI 123 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 579 GGTCTC GGTCTC(1/5)
BsaWI 367 TCCGGA [AT]^CCGG[AT]
BseAI 367 TCCGGA T^CCGGA
BsgI 247 GTGCAG GTGCAG(16/14)
BsiEI 5 CGACCG CG[GA][CT]^CG
BsmFI 417 GTCCC GGGAC(10/14)
BsoBI 419 CCCGGG C^[CT]CG[GA]G
BspEI 367 TCCGGA T^CCGGA
BssHII 313 GCGCGC G^CGCGC
BstBI 290 TTCGAA TT^CGAA
DdeI 485 CTGAG C^T[ACGT]AG
DraII 415 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 689 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 254 TGGCCA [CT]^GGCC[GA]
EcoO109I 415 GGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 188 AGCGCC [GA]GCGC^[CT]
HgaI 162 GACGC GACGC(5/10)
HphI 156 GGTGA GGTGA(8/7)
MaeII 559 ACGT A^CGT
MamI 123 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 623 CAATTG C^AATTG
MluNI 254 TGGCCA TGG^CCA
MroI 367 TCCGGA T^CCGGA
MscI 254 TGGCCA TGG^CCA
MseI 382 TTAA T^TAA
MslI 534 CATGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 623 CAATTG C^AATTG
NspI 533 ACATGC [GA]CATG^[CT]
NspV 290 TTCGAA TT^CGAA
PflFI 162 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 177 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 415 GGGTCCC [GA]G^G[AT]CC[CT]
SexAI 575 ACCAGGT A^CC[AT]GGT
SfuI 290 TTCGAA TT^CGAA
SmaI 419 CCCGGG CCC^GGG
Tru9I 382 TTAA T^TAA
Tsp509I 624 AATT ^AATT
Tth111I 162 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 177 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 576 CCAGGTCTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 419 CCCGGG C^CCGGG
XmaI 419 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 181 CGCG CG^CG
314 CGCG
AspHI 22 GAGCAC G[AT]GC[AT]^C
511 GTGCAC
BbsI 159 GAAGAC GAAGAC(2/6)
448 GAAGAC
BbvI 249 GCAGC GCAGC(8/12)
136 GCTGC
BmyI 22 GAGCAC G[AGT]GC[ACT]^C
511 GTGCAC
BpuAI 159 GAAGAC GAAGAC(2/6)
448 GAAGAC
BsiHKAI 22 GAGCAC G[AT]GC[AT]^C
511 GTGCAC
BsiYI 96 CCAGAGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
177 CCAGCGCGTGG
BslI 96 CCAGAGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
177 CCAGCGCGTGG
BsmAI 168 GTCTC GTCTC(1/5)
580 GTCTC
Bsp1286I 22 GAGCAC G[AGT]GC[ACT]^C
511 GTGCAC
BstF5I 129 CATCC GGATG(2/0)
501 CATCC
BstUI 181 CGCG CG^CG
314 CGCG
BstXI 11 CCATGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
622 CCAATTGAGTGG
BstYI 200 AGATCC [GA]^GATC[CT]
327 AGATCT
DpnII 201 GATC ^GATC
328 GATC
Fnu4HI 136 GCTGC GC^[ACGT]GC
249 GCAGC
FokI 129 CATCC GGATG(9/13)
501 CATCC
ItaI 136 GCTGC GC^[ACGT]GC
249 GCAGC
MaeIII 236 GTGAC ^GT[ACGT]AC
605 GTGAC
MboI 201 GATC ^GATC
328 GATC
MflI 200 AGATCC [GA]^GATC[CT]
327 AGATCT
MspA1I 76 CAGCTG C[AC]G^C[GT]G
250 CAGCTG
MvnI 181 CGCG CG^CG
314 CGCG
NdeII 201 GATC ^GATC
328 GATC
PstI 137 CTGCAG CTGCA^G
193 CTGCAG
PvuII 76 CAGCTG CAG^CTG
250 CAGCTG
Sau3AI 201 GATC ^GATC
328 GATC
SfaNI 81 GATGC GCATC(5/9)
301 GATGC
TaqI 41 TCGA T^CGA
291 TCGA
TseI 136 GCTGC G^C[AT]GC
249 GCAGC
Tsp45I 236 GTGAC ^GT[GC]AC
605 GTGAC
TthHB8I 41 TCGA T^CGA
291 TCGA
XhoII 200 AGATCC [GA]^GATC[CT]
327 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI AvaII BcnI BsaJI
BssKI BstNI Cac8I CfoI Cfr13I
DsaI EcoRII HaeIII HapII HhaI
HinP1I HpaII MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
RsaI Sau96I ScrFI SfcI TspRI