(Circular) (Six-base) MAPSORT of: pFN21AB7940.seq Check: 3799 from: 1 to: 5425 pFN21AB7940 5425 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3235 278 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 1937 Size: 831 4594 Acc65I G'GTAC_C Cuts at: 2746 2746 Size: 5425 AclI AA'CG_TT Cuts at: 1563 3552 3925 1563 Size: 1989 373 3063 Fragments arranged by size: 3063 1989 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 2357 3488 4872 5140 1632 Size: 419 306 1131 1384 268 1917 Fragments arranged by size: 1917 1384 1131 419 306 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5228 AflIII A'CryG_T Cuts at: 1203 4324 5301 1203 Size: 3121 977 1327 Fragments arranged by size: 3121 1327 977 AgeI A'CCGG_T Cuts at: 4256 4256 Size: 5425 AhdI GACnn_n'nnGTC Cuts at: 4154 4154 Size: 5425 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5425 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5425 AlwNI CAG_nnn'CTG Cuts at: 3168 4740 3168 Size: 1572 3853 ApaLI G'TGCA_C Cuts at: 2516 3482 4638 2516 Size: 966 1156 3303 Fragments arranged by size: 3303 1156 966 ApoI r'AATT_y Cuts at: 1493 2734 2960 3112 5221 1493 Size: 1241 226 152 2109 1697 Fragments arranged by size: 2109 1697 1241 226 152 AseI AT'TA_AT Cuts at: 160 1032 3979 160 Size: 872 2947 1606 Fragments arranged by size: 2947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 AvaI C'yCGr_G Cuts at: 1870 1943 2424 2750 1870 Size: 73 481 326 4545 Fragments arranged by size: 4545 481 326 73 BamHI G'GATC_C Cuts at: 2755 3122 2755 Size: 367 5058 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2746 4201 618 Size: 325 217 627 959 1455 1842 Fragments arranged by size: 1842 1455 959 627 325 217 BanII G_rGCy'C Cuts at: 1391 2744 1391 Size: 1353 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 2460 928 Size: 971 272 289 3893 Fragments arranged by size: 3893 971 289 272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3616 3650 1903 Size: 34 1679 34 3678 Fragments arranged by size: 3678 1679 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3317 4527 3317 Size: 1210 4215 BclI T'GATC_A Cuts at: 1548 1548 Size: 5425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4036 136 Size: 107 122 71 631 605 2364 1525 Fragments arranged by size: 2364 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2332 5420 2332 Size: 3088 2337 BlpI GC'TnA_GC Cuts at: 2842 2842 Size: 5425 Bme1580I G_kGCm'C Cuts at: 1790 2520 3486 4642 1790 Size: 730 966 1156 2573 Fragments arranged by size: 2573 1156 966 730 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5425 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3156 4114 442 Size: 416 53 83 399 24 1739 958 1753 Fragments arranged by size: 1753 1739 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5425 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2170 2170 Size: 5425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2160 4085 1239 Size: 921 1925 2579 Fragments arranged by size: 2579 1925 921 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3550 4415 4713 3550 Size: 865 298 4262 Fragments arranged by size: 4262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2590 4088 882 Size: 617 1091 1498 2219 Fragments arranged by size: 2219 1498 1091 617 BsaAI yAC'GTr Cuts at: 493 1204 2565 3216 493 Size: 711 1361 651 2702 Fragments arranged by size: 2702 1361 711 651 BsaBI GATnn'nnATC Cuts at: 2132 5419 2132 Size: 3287 2138 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3232 3614 4249 275 Size: 53 83 186 936 1699 382 635 1451 Fragments arranged by size: 1699 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2372 3858 4256 4530 4677 5088 2372 Size: 1486 398 274 147 411 2709 Fragments arranged by size: 2709 1486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2158 2188 2570 2600 1178 Size: 30 165 30 755 30 382 30 4003 Fragments arranged by size: 4003 755 382 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5425 BseYI C'CCAG_C Cuts at: 4628 5124 4628 Size: 496 4929 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 1163 Size: 573 537 4315 Fragments arranged by size: 4315 573 537 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2875 3636 4664 1540 Size: 275 186 12 862 761 1028 2301 Fragments arranged by size: 2301 1028 862 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2031 2520 3486 3571 4642 2031 Size: 489 966 85 1071 2814 Fragments arranged by size: 2814 1071 966 489 85 BsmI GAATG_Cn' Cuts at: 2936 3029 2936 Size: 93 5332 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2520 2744 3486 3571 4642 1391 Size: 399 241 489 224 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 489 399 241 224 85 BspEI T'CCGG_A Cuts at: 2372 5088 2372 Size: 2716 2709 BspHI T'CATG_A Cuts at: 3314 5044 3314 Size: 1730 3695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2780 844 Size: 1936 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3312 1394 Size: 429 1489 3507 Fragments arranged by size: 3507 1489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3920 4094 65 Size: 1183 2672 174 1396 Fragments arranged by size: 2672 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4069 4256 1700 Size: 110 2259 187 2869 Fragments arranged by size: 2869 2259 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5425 BssHII G'CGCG_C Cuts at: 1925 2318 1925 Size: 393 5032 BssSI C'ACGA_G Cuts at: 3485 4497 3485 Size: 1012 4413 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5425 BstBI TT'CG_AA Cuts at: 2296 4229 2296 Size: 1933 3492 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5425 BstXI CCAn_nnnn'nTGG Cuts at: 2023 2634 2023 Size: 611 4814 BstYI r'GATC_y Cuts at: 1972 2205 2332 2755 3122 3507 3524 4976 5062 Size: 233 127 423 367 385 17 1452 86 Cuts at: 5062 5074 5420 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 423 385 367 346 233 127 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2157 2544 2693 513 Size: 552 939 153 387 149 3245 Fragments arranged by size: 3245 939 552 387 153 149 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4686 Fragments arranged by size: 4686 683 56 BtsI GCAGTG_nn' Cuts at: 2362 2941 3735 3755 2362 Size: 579 794 20 4032 Fragments arranged by size: 4032 794 579 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5390 DraI TTT'AAA Cuts at: 2729 2729 Size: 5425 DraIII CAC_nnn'GTG Cuts at: 2699 2699 Size: 5425 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4432 809 Size: 507 3116 1802 Fragments arranged by size: 3116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2872 3761 Size: 54 1425 50 275 18 429 613 889 Cuts at: 3761 5390 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 613 429 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2872 1537 Size: 275 1060 4090 Fragments arranged by size: 4090 1060 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3355 1353 Size: 2002 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4012 4386 4532 387 Size: 1064 138 2423 374 146 1280 Fragments arranged by size: 2423 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2160 2357 3488 4085 4872 5140 Size: 393 419 109 197 1131 597 787 268 Cuts at: 5140 1239 Size: 1524 Fragments arranged by size: 1524 1131 787 597 419 393 268 197 109 EcoO109I rG'GnC_Cy Cuts at: 2421 2421 Size: 5425 EcoRI G'AATT_C Cuts at: 2734 3112 2734 Size: 378 5047 FspI TGC'GCA Cuts at: 3931 3931 Size: 5425 HaeII r_GCGC'y Cuts at: 1678 2197 4572 1678 Size: 519 2375 2531 Fragments arranged by size: 2531 2375 519 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2158 2190 4066 4098 4140 Size: 32 135 32 586 32 1876 32 42 Cuts at: 4140 4172 1373 Size: 32 2626 Fragments arranged by size: 2626 1876 586 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2769 3015 669 Size: 988 281 831 246 3079 Fragments arranged by size: 3079 988 831 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5425 HpaI GTT'AAC Cuts at: 3015 3015 Size: 5425 KpnI G_GTAC'C Cuts at: 2750 2750 Size: 5425 MfeI C'AATT_G Cuts at: 2628 3024 2628 Size: 396 5029 MluI A'CGCG_T Cuts at: 5301 5301 Size: 5425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4539 4723 1305 Size: 3234 184 2007 Fragments arranged by size: 3234 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 10 Size: 54 1425 343 429 3174 Fragments arranged by size: 3174 1425 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 3383 3742 3901 518 Size: 648 1377 840 359 159 2042 Fragments arranged by size: 2042 1377 840 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2257 2841 3518 4666 4911 1606 Size: 477 174 584 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 584 477 245 174 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3934 Fragments arranged by size: 3934 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5425 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5425 NotI GC'GGCC_GC Cuts at: 2872 2872 Size: 5425 NspI r_CATG'y Cuts at: 1132 1229 2542 2783 4328 1132 Size: 97 1313 241 1545 2229 Fragments arranged by size: 2229 1545 1313 241 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5425 PciI A'CATG_T Cuts at: 4324 4324 Size: 5425 PflMI CCAn_nnn'nTGG Cuts at: 1108 2188 1108 Size: 1080 4345 PfoI T'CCnGG_A Cuts at: 2703 2703 Size: 5425 PmeI GTTT'AAAC Cuts at: 2729 2729 Size: 5425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3494 3526 5007 5039 3494 Size: 32 1481 32 3880 Fragments arranged by size: 3880 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2421 2421 Size: 5425 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5425 PsiI TTA'TAA Cuts at: 2995 2995 Size: 5425 PstI C_TGCA'G Cuts at: 830 2146 2202 2777 830 Size: 1316 56 575 3478 Fragments arranged by size: 3478 1316 575 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5425 PvuII CAG'CTG Cuts at: 2083 2257 2083 Size: 174 5251 SalI G'TCGA_C Cuts at: 1936 2767 1936 Size: 831 4594 SanDI GG'GwC_CC Cuts at: 2421 2421 Size: 5425 SbfI CC_TGCA'GG Cuts at: 2777 2777 Size: 5425 ScaI AGT'ACT Cuts at: 3673 5385 3673 Size: 1712 3713 SexAI A'CCwGG_T Cuts at: 2580 2580 Size: 5425 SfcI C'TryA_G Cuts at: 826 1045 2142 2198 2218 2773 3908 4589 4780 Size: 219 1097 56 20 555 1135 681 191 Cuts at: 4780 826 Size: 1471 Fragments arranged by size: 1471 1135 1097 681 555 219 191 56 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5425 SmaI CCC'GGG Cuts at: 1872 2426 2752 1872 Size: 554 326 4545 Fragments arranged by size: 4545 554 326 SmlI C'TyrA_G Cuts at: 820 1017 1943 3529 4430 4692 820 Size: 197 926 1586 901 262 1553 Fragments arranged by size: 1586 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3216 493 Size: 2723 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5425 SphI G_CATG'C Cuts at: 1132 2783 1132 Size: 1651 3774 SspI AAT'ATT Cuts at: 5 52 3349 5 Size: 47 3297 2081 Fragments arranged by size: 3297 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5425 StyI C'CwwG_G Cuts at: 513 1065 2004 2864 513 Size: 552 939 860 3074 Fragments arranged by size: 3074 939 860 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3452 3469 3622 3807 4252 325 Size: 1255 1872 17 153 185 445 1498 Fragments arranged by size: 1872 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2019 2148 3671 Size: 274 80 33 51 1443 42 129 1523 Cuts at: 3671 5383 96 Size: 1712 138 Fragments arranged by size: 1712 1523 1443 274 138 129 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2570 2602 2685 2717 5385 5417 2570 Size: 32 83 32 2668 32 2578 Fragments arranged by size: 2668 2578 83 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2170 1316 Size: 854 4571 XbaI T'CTAG_A Cuts at: 2761 2761 Size: 5425 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2588 2588 Size: 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5425 XmaI C'CCGG_G Cuts at: 1870 2424 2750 1870 Size: 554 326 4545 Fragments arranged by size: 4545 554 326 XmnI GAAnn'nnTTC Cuts at: 3554 3554 Size: 5425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3233 276 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI