(Circular) (Six-base) MAPSORT of: pFN21AB7939.seq Check: 574 from: 1 to: 5731 pFN21AB7939 5731 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2800 2800 Size: 5731 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3074 1937 Size: 1137 4594 Acc65I G'GTAC_C Cuts at: 3052 3052 Size: 5731 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2073 2138 2274 3794 5178 5446 1632 Size: 441 65 136 1520 1384 268 1917 Fragments arranged by size: 1917 1520 1384 441 268 136 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 4630 5607 1203 Size: 3427 977 1327 Fragments arranged by size: 3427 1327 977 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5731 AlwNI CAG_nnn'CTG Cuts at: 2096 2739 3474 5046 2096 Size: 643 735 1572 2781 Fragments arranged by size: 2781 1572 735 643 ApaLI G'TGCA_C Cuts at: 3788 4944 3788 Size: 1156 4575 ApoI r'AATT_y Cuts at: 1493 2147 2667 3040 3266 3418 5527 1493 Size: 654 520 373 226 152 2109 1697 Fragments arranged by size: 2109 1697 654 520 373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2277 3056 1870 Size: 73 334 779 4545 Fragments arranged by size: 4545 779 334 73 BamHI G'GATC_C Cuts at: 2128 2717 3061 3428 2128 Size: 589 344 367 4431 Fragments arranged by size: 4431 589 367 344 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2691 3052 4507 618 Size: 325 217 627 904 361 1455 1842 Fragments arranged by size: 1842 1455 904 627 361 325 217 BanII G_rGCy'C Cuts at: 1391 2537 3050 1391 Size: 1146 513 4072 Fragments arranged by size: 4072 1146 513 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 2079 2262 2505 928 Size: 971 116 64 183 243 4154 Fragments arranged by size: 4154 971 243 183 116 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3922 3956 1903 Size: 34 1985 34 3678 Fragments arranged by size: 3678 1985 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4342 136 Size: 107 122 71 631 605 2670 1525 Fragments arranged by size: 2670 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2292 2753 5726 2292 Size: 461 2973 2297 Fragments arranged by size: 2973 2297 461 BlpI GC'TnA_GC Cuts at: 3148 3148 Size: 5731 Bme1580I G_kGCm'C Cuts at: 1790 3792 4948 1790 Size: 2002 1156 2573 Fragments arranged by size: 2573 2002 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3462 4420 442 Size: 416 53 83 399 24 2045 958 1753 Fragments arranged by size: 2045 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3004 4391 1239 Size: 1765 1387 2579 Fragments arranged by size: 2579 1765 1387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2631 3856 4721 5019 2631 Size: 1225 865 298 3343 Fragments arranged by size: 3343 1225 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4394 882 Size: 617 2895 2219 Fragments arranged by size: 2895 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3522 493 Size: 711 2318 2702 Fragments arranged by size: 2702 2318 711 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3538 3920 4555 275 Size: 53 83 186 936 2005 382 635 1451 Fragments arranged by size: 2005 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4164 4562 4836 4983 5394 4164 Size: 398 274 147 411 4501 Fragments arranged by size: 4501 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5506 Fragments arranged by size: 5506 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2734 2795 2986 1186 Size: 1548 61 191 3931 Fragments arranged by size: 3931 1548 191 61 BseYI C'CCAG_C Cuts at: 4934 5430 4934 Size: 496 5235 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3181 3942 4970 1540 Size: 275 186 1180 761 1028 2301 Fragments arranged by size: 2301 1180 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3792 3877 4948 3792 Size: 85 1071 4575 Fragments arranged by size: 4575 1071 85 BsmI GAATG_Cn' Cuts at: 2036 3242 3335 2036 Size: 1206 93 4432 Fragments arranged by size: 4432 1206 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2537 3050 3792 3877 4948 1391 Size: 399 747 513 742 85 1071 2174 Fragments arranged by size: 2174 1071 747 742 513 399 85 BspEI T'CCGG_A Cuts at: 5394 5394 Size: 5731 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2800 3086 844 Size: 1956 286 3489 Fragments arranged by size: 3489 1956 286 BsrBI CCG'CTC Cuts at: 1394 1823 3618 1394 Size: 429 1795 3507 Fragments arranged by size: 3507 1795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4226 4400 65 Size: 1183 2978 174 1396 Fragments arranged by size: 2978 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4375 4562 1700 Size: 110 2565 187 2869 Fragments arranged by size: 2869 2565 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5731 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5731 BssSI C'ACGA_G Cuts at: 2961 3791 4803 2961 Size: 830 1012 3889 Fragments arranged by size: 3889 1012 830 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5731 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 2068 1184 Size: 884 4847 BstYI r'GATC_y Cuts at: 1972 2128 2292 2717 2753 3061 3428 3813 3830 Size: 156 164 425 36 308 367 385 17 Cuts at: 3830 5282 5368 5380 5726 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 425 385 367 346 308 164 156 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2862 2862 Size: 5731 BtgI C'CryG_G Cuts at: 513 1065 2239 2722 513 Size: 552 1174 483 3522 Fragments arranged by size: 3522 1174 552 483 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2420 487 Size: 683 56 1194 3798 Fragments arranged by size: 3798 1194 683 56 BtsI GCAGTG_nn' Cuts at: 3247 4041 4061 3247 Size: 794 20 4917 Fragments arranged by size: 4917 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3035 3035 Size: 5731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4738 809 Size: 507 3422 1802 Fragments arranged by size: 3422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2924 3178 4067 Size: 54 1425 50 275 18 1094 254 889 Cuts at: 4067 5696 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1094 889 275 254 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3178 1537 Size: 275 1366 4090 Fragments arranged by size: 4090 1366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2889 3661 1353 Size: 1536 772 3423 Fragments arranged by size: 3423 1536 772 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4318 4692 4838 387 Size: 1064 138 2729 374 146 1280 Fragments arranged by size: 2729 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2138 2274 3004 3794 4391 5178 Size: 393 441 65 136 730 790 597 787 Cuts at: 5178 5446 1239 Size: 268 1524 Fragments arranged by size: 1524 790 787 730 597 441 393 268 136 65 EcoO109I rG'GnC_Cy Cuts at: 2093 2093 Size: 5731 EcoRI G'AATT_C Cuts at: 2667 3040 3418 2667 Size: 373 378 4980 Fragments arranged by size: 4980 378 373 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2314 2623 2314 Size: 309 5422 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 4878 1678 Size: 3200 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2310 2342 2402 2434 2729 Size: 32 135 32 738 32 60 32 295 Cuts at: 2729 2761 4372 4404 4446 4478 1373 Size: 32 1611 32 42 32 2626 Fragments arranged by size: 2626 1611 738 295 135 60 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5731 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KpnI G_GTAC'C Cuts at: 3056 3056 Size: 5731 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2250 2479 4845 5029 1305 Size: 945 229 2366 184 2007 Fragments arranged by size: 2366 2007 945 229 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2926 10 Size: 54 1425 343 1094 2815 Fragments arranged by size: 2815 1425 1094 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2463 3689 4048 4207 518 Size: 648 1297 1226 359 159 2042 Fragments arranged by size: 2042 1297 1226 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3015 3147 3824 4972 5217 1606 Size: 1409 132 677 1148 245 2120 Fragments arranged by size: 2120 1409 1148 677 245 132 NcoI C'CATG_G Cuts at: 513 1065 2239 513 Size: 552 1174 4005 Fragments arranged by size: 4005 1174 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 2468 2715 3089 4634 1132 Size: 97 1239 247 374 1545 2229 Fragments arranged by size: 2229 1545 1239 374 247 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5731 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5731 PfoI T'CCnGG_A Cuts at: 2981 2981 Size: 5731 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PstI C_TGCA'G Cuts at: 830 2800 2846 2879 3083 830 Size: 1970 46 33 204 3478 Fragments arranged by size: 3478 1970 204 46 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 PvuII CAG'CTG Cuts at: 3015 3015 Size: 5731 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 2796 2842 2875 3079 4214 4895 5086 Size: 219 1751 46 33 204 1135 681 191 Cuts at: 5086 826 Size: 1471 Fragments arranged by size: 1751 1471 1135 681 219 204 191 46 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 2279 3058 1872 Size: 407 779 4545 Fragments arranged by size: 4545 779 407 SmlI C'TyrA_G Cuts at: 820 1017 1943 2646 3835 4736 4998 820 Size: 197 926 703 1189 901 262 1553 Fragments arranged by size: 1553 1189 926 901 703 262 197 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 3089 1132 Size: 1957 3774 SrfI GCCC'GGGC Cuts at: 2279 2279 Size: 5731 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5731 StyI C'CwwG_G Cuts at: 513 1065 2239 2889 2991 3027 3170 513 Size: 552 1174 650 102 36 143 3074 Fragments arranged by size: 3074 1174 650 552 143 102 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3758 3775 3928 4113 4558 325 Size: 1255 2178 17 153 185 445 1498 Fragments arranged by size: 2178 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2412 3977 5689 Size: 274 80 33 51 1443 435 1565 1712 Cuts at: 5689 96 Size: 138 Fragments arranged by size: 1712 1565 1443 435 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2436 2468 5691 5723 2436 Size: 32 3223 32 2444 Fragments arranged by size: 3223 2444 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2369 2685 2369 Size: 316 5415 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 2277 3056 1870 Size: 407 779 4545 Fragments arranged by size: 4545 779 407 XmnI GAAnn'nnTTC Cuts at: 2151 3860 2151 Size: 1709 4022 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI