(Circular) (Six-base) MAPSORT of: pFN21AB7937.seq Check: 4480 from: 1 to: 5833 pFN21AB7937 5833 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2435 2605 2917 2435 Size: 170 312 5351 Fragments arranged by size: 5351 312 170 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2477 2868 2937 3896 5280 5548 1632 Size: 845 391 69 959 1384 268 1917 Fragments arranged by size: 1917 1384 959 845 391 268 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2409 1184 Size: 1225 4608 AlwNI CAG_nnn'CTG Cuts at: 2620 3576 5148 2620 Size: 956 1572 3305 Fragments arranged by size: 3305 1572 956 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 2476 3053 3142 3368 3520 5629 1493 Size: 983 577 89 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 577 226 152 89 AseI AT'TA_AT Cuts at: 160 1032 2070 4387 160 Size: 872 1038 2317 1606 Fragments arranged by size: 2317 1606 1038 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 3158 1870 Size: 73 1215 4545 Fragments arranged by size: 4545 1215 73 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2516 3154 4609 618 Size: 325 217 627 729 638 1455 1842 Fragments arranged by size: 1842 1455 729 638 627 325 217 BanII G_rGCy'C Cuts at: 1391 2943 3152 1391 Size: 1552 209 4072 Fragments arranged by size: 4072 1552 209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2877 2901 928 Size: 971 978 24 3860 Fragments arranged by size: 3860 978 971 24 BbvCI CC'TCA_GC Cuts at: 2280 2295 2538 2673 2280 Size: 15 243 135 5440 Fragments arranged by size: 5440 243 135 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4024 4058 1903 Size: 34 2087 34 3678 Fragments arranged by size: 3678 2087 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2830 3725 4935 2830 Size: 895 1210 3728 Fragments arranged by size: 3728 1210 895 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BfrBI ATG'CAT Cuts at: 2396 2396 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4444 136 Size: 107 122 71 631 605 2772 1525 Fragments arranged by size: 2772 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2048 5828 2048 Size: 3780 2053 BlpI GC'TnA_GC Cuts at: 2274 3250 2274 Size: 976 4857 Bme1580I G_kGCm'C Cuts at: 1790 2521 3894 5050 1790 Size: 731 1373 1156 2573 Fragments arranged by size: 2573 1373 1156 731 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2841 3564 4522 Size: 416 53 83 399 24 1424 723 958 Cuts at: 4522 442 Size: 1753 Fragments arranged by size: 1753 1424 958 723 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2655 4493 1239 Size: 1416 1838 2579 Fragments arranged by size: 2579 1838 1416 Bpu10I CC'TnA_GC Cuts at: 2280 2295 2431 2538 2673 2280 Size: 15 136 107 135 5440 Fragments arranged by size: 5440 136 135 107 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2688 3047 3958 4823 5121 2688 Size: 359 911 865 298 3400 Fragments arranged by size: 3400 911 865 359 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4496 882 Size: 617 2997 2219 Fragments arranged by size: 2997 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3640 4022 4657 275 Size: 53 83 186 936 2107 382 635 1451 Fragments arranged by size: 2107 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2788 2818 1178 Size: 30 165 30 1385 30 4193 Fragments arranged by size: 4193 1385 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2616 2903 1186 Size: 1430 287 4116 Fragments arranged by size: 4116 1430 287 BseYI C'CCAG_C Cuts at: 2232 2310 2784 5036 5532 2232 Size: 78 474 2252 496 2533 Fragments arranged by size: 2533 2252 496 474 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5260 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3894 3979 5050 3894 Size: 85 1071 4677 Fragments arranged by size: 4677 1071 85 BsmI GAATG_Cn' Cuts at: 2398 3344 3437 2398 Size: 946 93 4794 Fragments arranged by size: 4794 946 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2521 2943 3152 3894 3979 5050 1391 Size: 399 731 422 209 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 731 422 399 209 85 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2305 2435 2605 2917 3188 844 Size: 1461 130 170 312 271 3489 Fragments arranged by size: 3489 1461 312 271 170 130 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4328 4502 65 Size: 1183 3080 174 1396 Fragments arranged by size: 3080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4477 4664 1700 Size: 110 2667 187 2869 Fragments arranged by size: 2869 2667 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 2441 794 Size: 1647 4186 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstYI r'GATC_y Cuts at: 1972 2048 3163 3530 3915 3932 5384 5470 5482 Size: 76 1115 367 385 17 1452 86 12 Cuts at: 5482 5828 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1115 385 367 346 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4342 Fragments arranged by size: 4342 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4143 4163 3349 Size: 794 20 5019 Fragments arranged by size: 5019 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2764 3280 4169 Size: 54 1425 50 275 18 934 516 889 Cuts at: 4169 5798 8 Size: 1629 43 Fragments arranged by size: 1629 1425 934 889 516 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2634 2931 3085 3763 1353 Size: 831 450 297 154 678 3423 Fragments arranged by size: 3423 831 678 450 297 154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2477 2655 2868 2937 3896 4493 5280 Size: 393 845 178 213 69 959 597 787 Cuts at: 5280 5548 1239 Size: 268 1524 Fragments arranged by size: 1524 959 845 787 597 393 268 213 178 69 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 4980 1678 Size: 3302 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2278 2310 2828 2860 2970 Size: 32 135 32 706 32 518 32 110 Cuts at: 2970 3002 4474 4506 4548 4580 1373 Size: 32 1472 32 42 32 2626 Fragments arranged by size: 2626 1472 706 518 135 110 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4947 5131 1305 Size: 3642 184 2007 Fragments arranged by size: 3642 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2766 10 Size: 54 1425 343 934 3077 Fragments arranged by size: 3077 1425 934 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2391 3791 4150 4309 518 Size: 648 1225 1400 359 159 2042 Fragments arranged by size: 2042 1400 1225 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2423 3249 3926 5074 5319 1606 Size: 817 826 677 1148 245 2120 Fragments arranged by size: 2120 1148 826 817 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4342 Fragments arranged by size: 4342 939 552 NdeI CA'TA_TG Cuts at: 387 2388 387 Size: 2001 3832 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NsiI A_TGCA'T Cuts at: 2398 2398 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 3191 4736 1132 Size: 97 1962 1545 2229 Fragments arranged by size: 2229 1962 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5833 PfoI T'CCnGG_A Cuts at: 2632 2632 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2657 1613 Size: 1044 4789 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2821 2853 2821 Size: 32 5801 PstI C_TGCA'G Cuts at: 830 2446 3185 830 Size: 1616 739 3478 Fragments arranged by size: 3478 1616 739 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 2423 2423 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SapI GCTCTTCn'nnn_ Cuts at: 2184 2931 2184 Size: 747 5086 SbfI CC_TGCA'GG Cuts at: 2446 3185 2446 Size: 739 5094 ScaI AGT'ACT Cuts at: 2118 4081 5793 2118 Size: 1963 1712 2158 Fragments arranged by size: 2158 1963 1712 SfcI C'TryA_G Cuts at: 826 1045 2418 2442 3181 4316 4997 5188 826 Size: 219 1373 24 739 1135 681 191 1471 Fragments arranged by size: 1471 1373 1135 739 681 219 191 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2703 3062 3937 4838 5100 820 Size: 197 926 760 359 875 901 262 1553 Fragments arranged by size: 1553 926 901 875 760 359 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2004 3272 513 Size: 552 939 1268 3074 Fragments arranged by size: 3074 1268 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3860 3877 4030 4215 4660 325 Size: 1255 2280 17 153 185 445 1498 Fragments arranged by size: 2280 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2116 2593 4079 Size: 274 80 33 51 1443 139 477 1486 Cuts at: 4079 5791 96 Size: 1712 138 Fragments arranged by size: 1712 1486 1443 477 274 139 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5793 5825 5793 Size: 32 5801 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI