(Circular) (Six-base) MAPSORT of: pFN21AB7920.seq Check: 7834 from: 1 to: 5743 pFN21AB7920 5743 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2757 2757 Size: 5743 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2510 3086 1937 Size: 573 576 4594 Fragments arranged by size: 4594 576 573 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2836 3806 5190 5458 1632 Size: 1204 970 1384 268 1917 Fragments arranged by size: 1917 1384 1204 970 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 3486 5058 3486 Size: 1572 4171 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 2146 2238 3052 3278 3430 5539 1493 Size: 653 92 814 226 152 2109 1697 Fragments arranged by size: 2109 1697 814 653 226 152 92 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2904 3068 1870 Size: 73 961 164 4545 Fragments arranged by size: 4545 961 164 73 BamHI G'GATC_C Cuts at: 2529 3073 3440 2529 Size: 544 367 4832 Fragments arranged by size: 4832 544 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2254 3064 4519 618 Size: 325 217 627 467 810 1455 1842 Fragments arranged by size: 1842 1455 810 627 467 325 217 BanII G_rGCy'C Cuts at: 1391 2340 2736 3062 1391 Size: 949 396 326 4072 Fragments arranged by size: 4072 949 396 326 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4772 BbvCI CC'TCA_GC Cuts at: 2250 2754 2250 Size: 504 5239 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2839 3635 4845 2839 Size: 796 1210 3737 Fragments arranged by size: 3737 1210 796 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4354 136 Size: 107 122 71 631 605 337 2345 1525 Fragments arranged by size: 2345 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5738 5738 Size: 5743 BlpI GC'TnA_GC Cuts at: 2604 3160 2604 Size: 556 5187 Bme1580I G_kGCm'C Cuts at: 1790 2570 3804 4960 1790 Size: 780 1234 1156 2573 Fragments arranged by size: 2573 1234 1156 780 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4432 442 Size: 416 53 83 399 24 2057 958 1753 Fragments arranged by size: 2057 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2249 1056 Size: 1193 4550 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2414 4403 1239 Size: 968 207 1989 2579 Fragments arranged by size: 2579 1989 968 207 Bpu10I CC'TnA_GC Cuts at: 2250 2754 2250 Size: 504 5239 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2750 3868 4733 5031 2750 Size: 1118 865 298 3462 Fragments arranged by size: 3462 1118 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 882 Size: 617 2907 2219 Fragments arranged by size: 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2494 3534 493 Size: 711 1290 1040 2702 Fragments arranged by size: 2702 1290 1040 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3550 3932 4567 275 Size: 53 83 186 936 2017 382 635 1451 Fragments arranged by size: 2017 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2105 2868 4176 4574 4848 4995 5406 2105 Size: 763 1308 398 274 147 411 2442 Fragments arranged by size: 2442 1308 763 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5518 Fragments arranged by size: 5518 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2771 1186 Size: 1585 4158 BseYI C'CCAG_C Cuts at: 4946 5442 4946 Size: 496 5247 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2816 1163 Size: 573 1080 4090 Fragments arranged by size: 4090 1080 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3804 3889 4960 3804 Size: 85 1071 4587 Fragments arranged by size: 4587 1071 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 BsmBI CGTCTCn'nnnn_ Cuts at: 2432 2432 Size: 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2340 2570 2736 3062 3804 3889 4960 Size: 399 550 230 166 326 742 85 1071 Cuts at: 4960 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 550 399 326 230 166 85 BspEI T'CCGG_A Cuts at: 2868 5406 2868 Size: 2538 3205 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2640 2757 3098 844 Size: 1796 117 341 3489 Fragments arranged by size: 3489 1796 341 117 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2077 2217 4238 4412 65 Size: 1183 829 140 2021 174 1396 Fragments arranged by size: 2021 1396 1183 829 174 140 BsrFI r'CCGG_y Cuts at: 1700 1810 3003 4387 4574 1700 Size: 110 1193 1384 187 2869 Fragments arranged by size: 2869 1384 1193 187 110 BsrGI T'GTAC_A Cuts at: 96 2286 96 Size: 2190 3553 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 2219 794 Size: 1425 4318 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstYI r'GATC_y Cuts at: 1972 2529 3073 3440 3825 3842 5294 5380 5392 Size: 557 544 367 385 17 1452 86 12 Cuts at: 5392 5738 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 557 544 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2511 2511 Size: 5743 BtgI C'CryG_G Cuts at: 513 1065 2004 2736 513 Size: 552 939 732 3520 Fragments arranged by size: 3520 939 732 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2584 487 Size: 683 56 1358 3646 Fragments arranged by size: 3646 1358 683 56 BtsI GCAGTG_nn' Cuts at: 3259 4053 4073 3259 Size: 794 20 4929 Fragments arranged by size: 4929 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DraIII CAC_nnn'GTG Cuts at: 2506 2783 2506 Size: 277 5466 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2617 2820 3190 Size: 54 1425 50 275 18 787 203 370 Cuts at: 3190 4079 5708 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 787 370 275 203 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3673 1353 Size: 2320 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2414 2836 3806 4403 5190 5458 Size: 393 575 207 422 970 597 787 268 Cuts at: 5458 1239 Size: 1524 Fragments arranged by size: 1524 970 787 597 575 422 393 268 207 EcoO109I rG'GnC_Cy Cuts at: 2278 2278 Size: 5743 EcoRI G'AATT_C Cuts at: 2238 3052 3430 2238 Size: 814 378 4551 Fragments arranged by size: 4551 814 378 EcoRV GAT'ATC Cuts at: 2593 2593 Size: 5743 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 3010 4890 1678 Size: 1332 1880 2531 Fragments arranged by size: 2531 1880 1332 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2308 2340 2398 Size: 32 135 32 593 32 111 32 58 Cuts at: 2398 2430 4384 4416 4458 4490 1373 Size: 32 1954 32 42 32 2626 Fragments arranged by size: 2626 1954 593 135 111 58 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3087 3333 669 Size: 988 281 1002 147 246 3079 Fragments arranged by size: 3079 1002 988 281 246 147 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2599 4857 5041 1305 Size: 1294 2258 184 2007 Fragments arranged by size: 2258 2007 1294 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2619 2822 10 Size: 54 1425 343 787 203 2931 Fragments arranged by size: 2931 1425 787 343 203 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 3701 4060 4219 518 Size: 648 1006 1529 359 159 2042 Fragments arranged by size: 2042 1529 1006 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2608 2753 3159 3836 4984 5229 1606 Size: 1002 145 406 677 1148 245 2120 Fragments arranged by size: 2120 1148 1002 677 406 245 145 NaeI GCC'GGC Cuts at: 3005 3005 Size: 5743 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 NdeI CA'TA_TG Cuts at: 387 2520 387 Size: 2133 3610 NgoMIV G'CCGG_C Cuts at: 3003 3003 Size: 5743 NheI G'CTAG_C Cuts at: 1052 2245 1052 Size: 1193 4550 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 3101 4646 1132 Size: 97 1872 1545 2229 Fragments arranged by size: 2229 1872 1545 97 PasI CC'CwG_GG Cuts at: 1348 2536 1348 Size: 1188 4555 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5743 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2278 2278 Size: 5743 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PstI C_TGCA'G Cuts at: 830 3095 830 Size: 2265 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2608 2753 2608 Size: 145 5598 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 2988 3991 5703 2988 Size: 1003 1712 3028 Fragments arranged by size: 3028 1712 1003 SexAI A'CCwGG_T Cuts at: 2779 2779 Size: 5743 SfcI C'TryA_G Cuts at: 826 1045 2417 3091 4226 4907 5098 826 Size: 219 1372 674 1135 681 191 1471 Fragments arranged by size: 1471 1372 1135 681 674 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 2906 3070 1872 Size: 1034 164 4545 Fragments arranged by size: 4545 1034 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 3847 4748 5010 820 Size: 197 926 786 1118 901 262 1553 Fragments arranged by size: 1553 1118 926 901 786 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 2004 2383 2806 3182 513 Size: 552 939 379 423 376 3074 Fragments arranged by size: 3074 939 552 423 379 376 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 3787 3940 4125 4570 325 Size: 1255 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2164 2286 2986 Size: 274 80 33 51 1443 187 122 700 Cuts at: 2986 3989 5701 96 Size: 1003 1712 138 Fragments arranged by size: 1712 1443 1003 700 274 187 138 122 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5703 5735 5703 Size: 32 5711 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2839 2839 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 2904 3068 1870 Size: 1034 164 4545 Fragments arranged by size: 4545 1034 164 XmnI GAAnn'nnTTC Cuts at: 2147 3872 2147 Size: 1725 4018 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI