Restriction map for
pFN21AB7916
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AflIII AhdI Alw44I AlwNI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvrII BalI BbeI BbrPI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HgaI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 940 GACGTC GACGT^C
AccI 769 GTAGAC GT^[AC][GT]AC
AcyI 940 GACGTC G[GA]^CG[CT]C
AgeI 735 ACCGGT A^CCGGT
AocI 382 CCTCAGG CC^T[ACGT]AGG
Aor51HI 832 AGCGCT AGC^GCT
ApaI 322 GGGCCC GGGCC^C
AviII 23 TGCGCA TGC^GCA
BamHI 164 GGATCC G^GATCC
BanI 1013 GGTGCC G^G[CT][GA]CC
BbsI 220 GAAGAC GAAGAC(2/6)
BbvCI 543 CCTCAGC CCTCAGC(-5/-2)
BcgI 174 CGAACTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 204 CTGGAG CTGGAG(16/14)
BpuAI 220 GAAGAC GAAGAC(2/6)
BsaHI 940 GACGTC G[GA]^CG[CT]C
BsaI 296 GAGACC GGTCTC(1/5)
BsaWI 735 ACCGGT [AT]^CCGG[AT]
BsmFI 634 GGGAC GGGAC(10/14)
BsmI 1025 GCATTC GAATGC(1/-1)
BsrFI 735 ACCGGT [GA]^CCGG[CT]
BstYI 164 GGATCC [GA]^GATC[CT]
Bsu36I 382 CCTCAGG CC^T[ACGT]AGG
Cfr10I 735 ACCGGT [GA]^CCGG[CT]
DraIII 682 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 807 TGGCCG [CT]^GGCC[GA]
EarI 904 GAAGAG CTCTTC(1/4)
Eco47III 832 AGCGCT AGC^GCT
Eco81I 382 CCTCAGG CC^T[ACGT]AGG
EcoNI 894 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 23 TGCGCA TGC^GCA
HaeII 832 AGCGCT [GA]GCGC^[CT]
Hin1I 940 GACGTC G[GA]^CG[CT]C
Ksp632I 904 GAAGAG CTCTTC(1/4)
MflI 164 GGATCC [GA]^GATC[CT]
MspA1I 478 CAGCGG C[AC]G^C[GT]G
NcoI 839 CCATGG C^CATGG
NspI 180 GCATGC [GA]CATG^[CT]
PinAI 735 ACCGGT A^CCGGT
SphI 180 GCATGC GCATG^C
TaqI 801 TCGA T^CGA
Tsp509I 284 AATT ^AATT
TthHB8I 801 TCGA T^CGA
XcmI 138 CCAGCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 164 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 551 AGCT AG^CT
879 AGCT
AvaI 191 CCCGGG C^[CT]CG[GA]G
428 CCCGGG
BfaI 416 CTAG C^TAG
581 CTAG
BsgI 84 CTGCAC GTGCAG(16/14)
875 GTGCAG
BsmAI 296 GAGAC GTCTC(1/5)
907 GAGAC
BsoBI 191 CCCGGG C^[CT]CG[GA]G
428 CCCGGG
CfoI 24 GCGC GCG^C
833 GCGC
DraII 321 AGGGCCC [GA]G^G[ACGT]CC[CT]
322 GGGCCCC
DrdI 202 GACTGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
803 GACGTGGCCGTC
DsaI 409 CCACGG C^C[GA][CT]GG
839 CCATGG
EcoO109I 321 AGGGCCC [GA]G^G[ACGT]CC[CT]
322 GGGCCCC
HhaI 24 GCGC GCG^C
833 GCGC
HinP1I 24 GCGC G^CGC
833 GCGC
MaeI 416 CTAG C^TAG
581 CTAG
MaeII 804 ACGT A^CGT
941 ACGT
MaeIII 272 GTCAC ^GT[ACGT]AC
938 GTGAC
PstI 851 CTGCAG CTGCA^G
969 CTGCAG
SfaNI 1004 GATGC GCATC(5/9)
974 GCATC
SfcI 851 CTGCAG C^T[GA][CT]AG
969 CTGCAG
SmaI 191 CCCGGG CCC^GGG
428 CCCGGG
Tsp45I 272 GTCAC ^GT[GC]AC
938 GTGAC
XmaCI 191 CCCGGG C^CCGGG
428 CCCGGG
XmaI 191 CCCGGG C^CCGGG
428 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AlwI AvaII BanII
BbvI BcnI BglI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI StyI TseI TspRI