(Circular) (Six-base) MAPSORT of: pFN21AB7916.seq Check: 8970 from: 1 to: 5740 pFN21AB7916 5740 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2949 3550 278 Size: 53 83 186 936 1413 601 2468 Fragments arranged by size: 2468 1413 936 601 186 83 53 AccI GT'mk_AC Cuts at: 1937 2775 3083 1937 Size: 838 308 4594 Fragments arranged by size: 4594 838 308 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2343 3803 5187 5455 1632 Size: 711 1460 1384 268 1917 Fragments arranged by size: 1917 1460 1384 711 268 AfeI AGC'GCT Cuts at: 2839 2839 Size: 5740 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 2740 4571 2740 Size: 1831 3909 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 3483 5055 3483 Size: 1572 4168 ApaI G_GGCC'C Cuts at: 2331 2331 Size: 5740 ApaLI G'TGCA_C Cuts at: 3797 4953 3797 Size: 1156 4584 ApoI r'AATT_y Cuts at: 1493 3049 3275 3427 5536 1493 Size: 1556 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 226 152 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 2196 2433 3065 1870 Size: 73 253 237 632 4545 Fragments arranged by size: 4545 632 253 237 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2235 2268 2235 Size: 33 5707 BamHI G'GATC_C Cuts at: 2169 3070 3437 2169 Size: 901 367 4472 Fragments arranged by size: 4472 901 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3018 3061 4516 618 Size: 325 217 627 1231 43 1455 1842 Fragments arranged by size: 1842 1455 1231 627 325 217 43 BanII G_rGCy'C Cuts at: 1391 2196 2331 2479 2757 2877 3059 1391 Size: 805 135 148 278 120 182 4072 Fragments arranged by size: 4072 805 278 182 148 135 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 928 Size: 971 333 4436 Fragments arranged by size: 4436 971 333 BbvCI CC'TCA_GC Cuts at: 2549 2549 Size: 5740 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2168 2202 3931 3965 1903 Size: 34 50 34 147 34 1729 34 3678 Fragments arranged by size: 3678 1729 147 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2516 2996 3027 Size: 107 122 71 631 605 844 480 31 Cuts at: 3027 4351 136 Size: 1324 1525 Fragments arranged by size: 1525 1324 844 631 605 480 122 107 71 31 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 2331 3801 4957 1790 Size: 541 1470 1156 2573 Fragments arranged by size: 2573 1470 1156 541 BmgBI CAC'GTC Cuts at: 1318 2810 1318 Size: 1492 4248 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2825 3471 4429 Size: 416 53 83 399 24 1408 646 958 Cuts at: 4429 442 Size: 1753 Fragments arranged by size: 1753 1408 958 646 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2739 2739 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2230 4400 1239 Size: 991 2170 2579 Fragments arranged by size: 2579 2170 991 Bpu10I CC'TnA_GC Cuts at: 2549 2549 Size: 5740 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2295 4403 882 Size: 617 796 2108 2219 Fragments arranged by size: 2219 2108 796 617 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2946 3547 3929 4564 Size: 53 83 186 936 1413 601 382 635 Cuts at: 4564 275 Size: 1451 Fragments arranged by size: 1451 1413 936 635 601 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2740 4173 4571 4845 4992 5403 2740 Size: 1433 398 274 147 411 3077 Fragments arranged by size: 3077 1433 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5515 Fragments arranged by size: 5515 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5740 BseYI C'CCAG_C Cuts at: 2142 2565 4943 5439 2142 Size: 423 2378 496 2443 Fragments arranged by size: 2443 2378 496 423 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2074 2901 1163 Size: 573 338 827 4002 Fragments arranged by size: 4002 827 573 338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3951 4979 1540 Size: 275 186 1189 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3801 3886 4957 3801 Size: 85 1071 4584 Fragments arranged by size: 4584 1071 85 BsmI GAATG_Cn' Cuts at: 3030 3251 3344 3030 Size: 221 93 5426 Fragments arranged by size: 5426 221 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2331 2479 2757 2877 3059 3801 Size: 399 406 135 148 278 120 182 742 Cuts at: 3801 3886 4957 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 406 399 278 182 148 135 120 85 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3095 844 Size: 2251 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3627 1394 Size: 429 1804 3507 Fragments arranged by size: 3507 1804 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4235 4409 65 Size: 1183 2987 174 1396 Fragments arranged by size: 2987 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2740 4384 4571 1700 Size: 110 930 1644 187 2869 Fragments arranged by size: 2869 1644 930 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5740 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstYI r'GATC_y Cuts at: 1972 2169 3070 3437 3822 3839 5291 5377 5389 Size: 197 901 367 385 17 1452 86 12 Cuts at: 5389 5735 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 901 385 367 346 197 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2388 2388 Size: 5740 BtgI C'CryG_G Cuts at: 513 1065 2414 2844 513 Size: 552 1349 430 3409 Fragments arranged by size: 3409 1349 552 430 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 3256 4050 4070 3256 Size: 794 20 4926 Fragments arranged by size: 4926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 3044 3044 Size: 5740 DraIII CAC_nnn'GTG Cuts at: 2692 2692 Size: 5740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2213 2814 4747 809 Size: 507 897 601 1933 1802 Fragments arranged by size: 1933 1802 897 601 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2812 3187 4076 Size: 54 1425 50 275 18 982 375 889 Cuts at: 4076 5705 8 Size: 1629 43 Fragments arranged by size: 1629 1425 982 889 375 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2904 3670 1353 Size: 1551 766 3423 Fragments arranged by size: 3423 1551 766 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2714 4327 4701 4847 387 Size: 1064 138 1125 1613 374 146 1280 Fragments arranged by size: 1613 1280 1125 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2230 2343 3803 4400 5187 5455 1239 Size: 393 598 113 1460 597 787 268 1524 Fragments arranged by size: 1524 1460 787 598 597 393 268 113 EcoNI CCTnn'n_nnAGG Cuts at: 2903 2903 Size: 5740 EcoO109I rG'GnC_Cy Cuts at: 2327 2328 2327 Size: 1 5739 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 FspI TGC'GCA Cuts at: 2030 4246 2030 Size: 2216 3524 HaeII r_GCGC'y Cuts at: 1678 2841 4887 1678 Size: 1163 2046 2531 Fragments arranged by size: 2531 2046 1163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4381 4413 4455 4487 1373 Size: 32 135 32 2809 32 42 32 2626 Fragments arranged by size: 2809 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3084 3330 669 Size: 988 281 1146 246 3079 Fragments arranged by size: 3079 1146 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2044 2287 2392 4854 5038 1305 Size: 739 243 105 2462 184 2007 Fragments arranged by size: 2462 2007 739 243 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3918 Fragments arranged by size: 3918 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3698 4057 4216 518 Size: 648 2532 359 159 2042 Fragments arranged by size: 2532 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2485 3156 3833 4981 5226 1606 Size: 879 671 677 1148 245 2120 Fragments arranged by size: 2120 1148 879 677 671 245 NcoI C'CATG_G Cuts at: 513 1065 2844 513 Size: 552 1779 3409 Fragments arranged by size: 3409 1779 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 2189 3098 4643 1132 Size: 97 960 909 1545 2229 Fragments arranged by size: 2229 1545 960 909 97 PasI CC'CwG_GG Cuts at: 1348 2470 3014 1348 Size: 1122 544 4074 Fragments arranged by size: 4074 1122 544 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5740 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PspOMI G'GGCC_C Cuts at: 2327 2327 Size: 5740 PstI C_TGCA'G Cuts at: 830 2860 2978 3092 830 Size: 2030 118 114 3478 Fragments arranged by size: 3478 2030 118 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 2856 2974 3088 4223 4904 5095 826 Size: 219 1811 118 114 1135 681 191 1471 Fragments arranged by size: 1811 1471 1135 681 219 191 118 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 2198 2435 3067 1872 Size: 326 237 632 4545 Fragments arranged by size: 4545 632 326 237 SmlI C'TyrA_G Cuts at: 820 1017 1943 3844 4745 5007 820 Size: 197 926 1901 901 262 1553 Fragments arranged by size: 1901 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 2189 3098 1132 Size: 1057 909 3774 Fragments arranged by size: 3774 1057 909 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2239 2514 2844 3179 513 Size: 552 1174 275 330 335 3074 Fragments arranged by size: 3074 1174 552 335 330 275 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3767 3784 3937 4122 4567 325 Size: 1255 2187 17 153 185 445 1498 Fragments arranged by size: 2187 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3986 5698 96 Size: 274 80 33 51 1443 2009 1712 138 Fragments arranged by size: 2009 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2150 2150 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 2196 2433 3065 1870 Size: 326 237 632 4545 Fragments arranged by size: 4545 632 326 237 XmnI GAAnn'nnTTC Cuts at: 3869 3869 Size: 5740 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2947 3548 276 Size: 53 83 186 936 1413 601 2468 Fragments arranged by size: 2468 1413 936 601 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI