Restriction map for
pFN21AB6285
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AviII BalI BamHI BbrPI BcgI
BciVI BclI BglI BglII BsaAI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BspMI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MaeII MamI
MluI MluNI MroI MscI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SmaI SnaBI
SphI Sse8387I SspI SwaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AocI 2508 CCTCAGG CC^T[ACGT]AGG
Asp700I 1954 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1051 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 256 CCTAGG C^CTAGG
BlnI 256 CCTAGG C^CTAGG
BlpI 2107 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2107 GCTCAGC GC^T[ACGT]AGC
BsgI 1427 GTGCAG GTGCAG(16/14)
BsiEI 80 CGGCCG CG[GA][CT]^CG
BsmBI 2351 GAGACG CGTCTC(1/5)
Bsp1407I 478 TGTACA T^GTACA
BsrBI 32 CCGCTC CCGCTC(-3/-3)
BsrGI 478 TGTACA T^GTACA
BssSI 2496 CACGAG CACGAG(-5/-1)
BstYI 2277 AGATCC [GA]^GATC[CT]
Bsu36I 2508 CCTCAGG CC^T[ACGT]AGG
CelII 2107 GCTCAGC GC^T[ACGT]AGC
EaeI 80 CGGCCG [CT]^GGCC[GA]
EagI 80 CGGCCG C^GGCCG
EarI 2394 GAAGAG CTCTTC(1/4)
EclXI 80 CGGCCG C^GGCCG
Eco52I 80 CGGCCG C^GGCCG
Eco81I 2508 CCTCAGG CC^T[ACGT]AGG
EcoNI 1603 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1873 GTCAAC GT[CT]^[GA]AC
HindII 1873 GTCAAC GT[CT]^[GA]AC
Ksp632I 2394 GAAGAG CTCTTC(1/4)
MflI 2277 AGATCC [GA]^GATC[CT]
MslI 111 CACTCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 71 CCATGG C^CATGG
NspI 817 GCATGT [GA]CATG^[CT]
PaeR7I 2558 CTCGAG C^TCGAG
PflFI 1051 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1192 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 199 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1335 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 1106 CTACAG C^T[GA][CT]AG
SgrAI 24 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 478 TGTACA T^GTACA
StuI 2079 AGGCCT AGG^CCT
Tth111I 1051 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1192 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2085 TCTAGA T^CTAGA
XhoI 2558 CTCGAG C^TCGAG
XhoII 2277 AGATCC [GA]^GATC[CT]
XmnI 1954 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 967 CTTAAG C^TTAAG
2245 CTTAAG
ApaI 1245 GGGCCC GGGCC^C
1533 GGGCCC
AseI 504 ATTAAT AT^TAAT
745 ATTAAT
AsnI 504 ATTAAT AT^TAAT
745 ATTAAT
AvaI 57 CTCGGG C^[CT]CG[GA]G
2558 CTCGAG
BanI 28 GGCGCC G^G[CT][GA]CC
127 GGCGCC
BbeI 28 GGCGCC GGCGC^C
127 GGCGCC
BbvCI 391 CCTCAGC CCTCAGC(-5/-2)
943 CCTCAGC
BfrI 967 CTTAAG C^TTAAG
2245 CTTAAG
BsaWI 114 TCCGGT [AT]^CCGG[AT]
222 TCCGGT
BseRI 269 CTCCTC GAGGAG(10/8)
389 CTCCTC
BsoBI 57 CTCGGG C^[CT]CG[GA]G
2558 CTCGAG
BstEII 207 GGTGACC G^GT[ACGT]ACC
564 GGTAACC
BstPI 207 GGTGACC G^GT[ACGT]ACC
564 GGTAACC
DraII 199 GGGACCC [GA]G^G[ACGT]CC[CT]
1533 GGGCCCT
EcoO109I 199 GGGACCC [GA]G^G[ACGT]CC[CT]
1533 GGGCCCT
EcoO65I 207 GGTGACC G^GT[ACGT]ACC
564 GGTAACC
KasI 28 GGCGCC G^GCGCC
127 GGCGCC
NaeI 7 GCCGGC GCC^GGC
25 GCCGGC
NarI 28 GGCGCC GG^CGCC
127 GGCGCC
NgoMIV 7 GCCGGC G^CCGGC
25 GCCGGC
PleI 1503 GACTC GAGTC(4/5)
2259 GAGTC
PshBI 504 ATTAAT AT^TAAT
745 ATTAAT
SacI 1973 GAGCTC GAGCT^C
2561 GAGCTC
SexAI 953 ACCTGGT A^CC[AT]GGT
2164 ACCAGGT
SfoI 28 GGCGCC GGC^GCC
127 GGCGCC
SpeI 1587 ACTAGT A^CTAGT
1708 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MfeI MnlI MseI MspA1I MspI
MunI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I