(Circular) (Six-base) MAPSORT of: pFN21AB6285.seq Check: 894 from: 1 to: 7291 pFN21AB6285 7291 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5101 278 Size: 53 83 186 936 3565 2468 Fragments arranged by size: 3565 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4634 1937 Size: 2697 4594 Acc65I G'GTAC_C Cuts at: 4612 4612 Size: 7291 AclI AA'CG_TT Cuts at: 1563 5418 5791 1563 Size: 3855 373 3063 Fragments arranged by size: 3855 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2690 3281 3411 3594 4607 5354 6738 7006 Size: 1058 591 130 183 1013 747 1384 268 Cuts at: 7006 1632 Size: 1917 Fragments arranged by size: 1917 1384 1058 1013 747 591 268 183 130 AflII C'TTAA_G Cuts at: 820 1017 2972 4250 820 Size: 197 1955 1278 3861 Fragments arranged by size: 3861 1955 1278 197 AflIII A'CryG_T Cuts at: 1203 6190 7167 1203 Size: 4987 977 1327 Fragments arranged by size: 4987 1327 977 AgeI A'CCGG_T Cuts at: 6122 6122 Size: 7291 AhdI GACnn_n'nnGTC Cuts at: 6020 6020 Size: 7291 AleI CACnn'nnGTG Cuts at: 1166 2120 1166 Size: 954 6337 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7291 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4040 4072 4040 Size: 32 7259 AlwNI CAG_nnn'CTG Cuts at: 2160 2188 2741 5034 6606 2160 Size: 28 553 2293 1572 2845 Fragments arranged by size: 2845 2293 1572 553 28 ApaI G_GGCC'C Cuts at: 3254 3542 3254 Size: 288 7003 ApaLI G'TGCA_C Cuts at: 5348 6504 5348 Size: 1156 6135 ApoI r'AATT_y Cuts at: 1493 2909 3768 3993 4184 4600 4826 4978 7087 Size: 1416 859 225 191 416 226 152 2109 Cuts at: 7087 1493 Size: 1697 Fragments arranged by size: 2109 1697 1416 859 416 226 225 191 152 AseI AT'TA_AT Cuts at: 160 1032 2510 2751 5845 160 Size: 872 1478 241 3094 1606 Fragments arranged by size: 3094 1606 1478 872 241 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7291 AvaI C'yCGr_G Cuts at: 1870 1943 2062 4563 4616 1870 Size: 73 119 2501 53 4545 Fragments arranged by size: 4545 2501 119 73 53 AvrII C'CTAG_G Cuts at: 2261 2261 Size: 7291 BamHI G'GATC_C Cuts at: 4621 4988 4621 Size: 367 6924 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2132 4612 6067 618 Size: 325 217 627 246 99 2480 1455 1842 Fragments arranged by size: 2480 1842 1455 627 325 246 217 99 BanII G_rGCy'C Cuts at: 1391 2172 2715 3254 3542 3982 4570 4610 1391 Size: 781 543 539 288 440 588 40 4072 Fragments arranged by size: 4072 781 588 543 539 440 288 40 BbeI G_GCGC'C Cuts at: 2037 2136 2037 Size: 99 7192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2934 3437 3689 928 Size: 971 1035 503 252 4530 Fragments arranged by size: 4530 1035 971 503 252 BbvCI CC'TCA_GC Cuts at: 2397 2949 2397 Size: 552 6739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5482 5516 1903 Size: 34 50 34 3461 34 3678 Fragments arranged by size: 3678 3461 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5183 6393 5183 Size: 1210 6081 BclI T'GATC_A Cuts at: 1548 1548 Size: 7291 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5902 136 Size: 107 122 71 631 605 4230 1525 Fragments arranged by size: 4230 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7286 7286 Size: 7291 BlpI GC'TnA_GC Cuts at: 4113 4708 4113 Size: 595 6696 Bme1580I G_kGCm'C Cuts at: 1790 3254 3542 5352 6508 1790 Size: 1464 288 1810 1156 2573 Fragments arranged by size: 2573 1810 1464 1156 288 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7291 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5022 5980 442 Size: 416 53 83 399 24 3605 958 1753 Fragments arranged by size: 3605 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7291 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2328 3174 3246 3495 3711 5951 1239 Size: 1089 846 72 249 216 2240 2579 Fragments arranged by size: 2579 2240 1089 846 249 216 72 Bpu10I CC'TnA_GC Cuts at: 2397 2949 2397 Size: 552 6739 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2425 2921 3106 5416 6281 6579 2425 Size: 496 185 2310 865 298 3137 Fragments arranged by size: 3137 2310 865 496 298 185 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2414 3352 4396 5954 882 Size: 617 915 938 1044 1558 2219 Fragments arranged by size: 2219 1558 1044 938 915 617 BsaAI yAC'GTr Cuts at: 493 1204 5082 493 Size: 711 3878 2702 Fragments arranged by size: 3878 2702 711 BsaBI GATnn'nnATC Cuts at: 7285 7285 Size: 7291 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 2133 2177 5098 Size: 53 83 186 936 501 99 44 2921 Cuts at: 5098 5480 6115 275 Size: 382 635 1451 Fragments arranged by size: 2921 1451 936 635 501 382 186 99 83 53 44 BsaWI w'CCGG_w Cuts at: 2119 2227 5724 6122 6396 6543 6954 2119 Size: 108 3497 398 274 147 411 2456 Fragments arranged by size: 3497 2456 411 398 274 147 108 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2131 2161 2620 2650 3079 Size: 30 165 30 728 30 459 30 429 Cuts at: 3079 3109 4040 4070 4541 4571 1178 Size: 30 931 30 471 30 3898 Fragments arranged by size: 3898 931 728 471 459 429 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2265 2385 1186 Size: 1079 120 6092 Fragments arranged by size: 6092 1079 120 BseYI C'CCAG_C Cuts at: 2181 3476 3531 3560 6494 6990 2181 Size: 1295 55 29 2934 496 2482 Fragments arranged by size: 2934 2482 1295 496 55 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3453 1163 Size: 573 1717 5001 Fragments arranged by size: 5001 1717 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 4741 5502 6530 1540 Size: 275 186 87 2653 761 1028 2301 Fragments arranged by size: 2653 2301 1028 761 275 186 87 BsiHKAI G_wGCw'C Cuts at: 2117 3982 4570 5352 5437 6508 2117 Size: 1865 588 782 85 1071 2900 Fragments arranged by size: 2900 1865 1071 782 588 85 BsmI GAATG_Cn' Cuts at: 4802 4895 4802 Size: 93 7198 BsmBI CGTCTCn'nnnn_ Cuts at: 4350 4350 Size: 7291 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2172 2715 3254 3542 3982 4570 Size: 399 327 55 543 539 288 440 588 Cuts at: 4570 4610 5352 5437 6508 1391 Size: 40 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 588 543 539 440 399 327 288 85 55 40 BspEI T'CCGG_A Cuts at: 6954 6954 Size: 7291 BspHI T'CATG_A Cuts at: 5180 6910 5180 Size: 1730 5561 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4646 844 Size: 3802 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2039 5178 1394 Size: 429 216 3139 3507 Fragments arranged by size: 3507 3139 429 216 BsrDI GCAATG_nn' Cuts at: 65 1248 2811 3038 3063 3625 5786 5960 65 Size: 1183 1563 227 25 562 2161 174 1396 Fragments arranged by size: 2161 1563 1396 1183 562 227 174 25 BsrFI r'CCGG_y Cuts at: 1700 1810 2012 2030 2098 5935 6122 1700 Size: 110 202 18 68 3837 187 2869 Fragments arranged by size: 3837 2869 202 187 110 68 18 BsrGI T'GTAC_A Cuts at: 96 2483 96 Size: 2387 4904 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7291 BssSI C'ACGA_G Cuts at: 4501 5351 6363 4501 Size: 850 1012 5429 Fragments arranged by size: 5429 1012 850 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7291 BstBI TT'CG_AA Cuts at: 6095 6095 Size: 7291 BstEII G'GTnAC_C Cuts at: 1184 2212 2569 1184 Size: 1028 357 5906 Fragments arranged by size: 5906 1028 357 BstYI r'GATC_y Cuts at: 1972 4282 4621 4988 5373 5390 6842 6928 6940 Size: 2310 339 367 385 17 1452 86 12 Cuts at: 6940 7286 1972 Size: 346 1977 Fragments arranged by size: 2310 1977 1452 385 367 346 339 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4514 4514 Size: 7291 BtgI C'CryG_G Cuts at: 513 1065 2076 2172 2199 3342 513 Size: 552 1011 96 27 1143 4462 Fragments arranged by size: 4462 1143 1011 552 96 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6552 Fragments arranged by size: 6552 683 56 BtsI GCAGTG_nn' Cuts at: 2532 3350 4807 5601 5621 2532 Size: 818 1457 794 20 4202 Fragments arranged by size: 4202 1457 818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7291 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7256 DraI TTT'AAA Cuts at: 4595 4595 Size: 7291 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6298 809 Size: 507 4982 1802 Fragments arranged by size: 4982 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 4738 5627 Size: 54 1425 50 275 18 255 2653 889 Cuts at: 5627 7256 8 Size: 1629 43 Fragments arranged by size: 2653 1629 1425 889 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2085 4738 1537 Size: 275 273 2653 4090 Fragments arranged by size: 4090 2653 275 273 EarI CTCTTCn'nnn_ Cuts at: 1353 4394 5221 1353 Size: 3041 827 3423 Fragments arranged by size: 3423 3041 827 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5878 6252 6398 387 Size: 1064 138 4289 374 146 1280 Fragments arranged by size: 4289 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3980 4568 3980 Size: 588 6703 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2328 2690 3174 3246 3281 3411 3495 Size: 393 696 362 484 72 35 130 84 Cuts at: 3495 3594 3711 4607 5354 5951 6738 7006 1239 Size: 99 117 896 747 597 787 268 1524 Fragments arranged by size: 1524 896 787 747 696 597 484 393 362 268 130 117 99 84 72 35 EcoNI CCTnn'n_nnAGG Cuts at: 3612 4586 3612 Size: 974 6317 EcoO109I rG'GnC_Cy Cuts at: 2205 3539 2205 Size: 1334 5957 EcoRI G'AATT_C Cuts at: 4600 4978 4600 Size: 378 6913 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4457 4457 Size: 7291 FspI TGC'GCA Cuts at: 5797 5797 Size: 7291 HaeII r_GCGC'y Cuts at: 1678 2037 2061 2096 2136 2238 6438 1678 Size: 359 24 35 40 102 4200 2531 Fragments arranged by size: 4200 2531 359 102 40 35 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2131 2163 4157 4189 5932 Size: 32 135 32 559 32 1994 32 1743 Cuts at: 5932 5964 6006 6038 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1994 1743 559 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3880 4635 4881 669 Size: 988 281 1942 755 246 3079 Fragments arranged by size: 3079 1942 988 755 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7291 HpaI GTT'AAC Cuts at: 4881 4881 Size: 7291 KasI G'GCGC_C Cuts at: 2033 2132 2033 Size: 99 7192 KpnI G_GTAC'C Cuts at: 4616 4616 Size: 7291 MfeI C'AATT_G Cuts at: 4107 4205 4328 4890 4107 Size: 98 123 562 6508 Fragments arranged by size: 6508 562 123 98 MluI A'CGCG_T Cuts at: 7167 7167 Size: 7291 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2786 6405 6589 1305 Size: 1481 3619 184 2007 Fragments arranged by size: 3619 2007 1481 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5469 Fragments arranged by size: 5469 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 5249 5608 5767 518 Size: 648 954 3129 359 159 2042 Fragments arranged by size: 3129 2042 954 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2256 2741 3281 3560 4294 4707 5384 6532 Size: 650 485 540 279 734 413 677 1148 Cuts at: 6532 6777 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 734 677 650 540 485 413 279 245 NaeI GCC'GGC Cuts at: 2014 2032 2014 Size: 18 7273 NarI GG'CG_CC Cuts at: 2034 2133 2034 Size: 99 7192 NcoI C'CATG_G Cuts at: 513 1065 2076 513 Size: 552 1011 5728 Fragments arranged by size: 5728 1011 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7291 NgoMIV G'CCGG_C Cuts at: 2012 2030 2012 Size: 18 7273 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7291 NotI GC'GGCC_GC Cuts at: 4738 4738 Size: 7291 NspI r_CATG'y Cuts at: 1132 1229 2826 4649 6194 1132 Size: 97 1597 1823 1545 2229 Fragments arranged by size: 2229 1823 1597 1545 97 PasI CC'CwG_GG Cuts at: 1348 2128 1348 Size: 780 6511 PciI A'CATG_T Cuts at: 6190 6190 Size: 7291 PflMI CCAn_nnn'nTGG Cuts at: 1108 3203 1108 Size: 2095 5196 PfoI T'CCnGG_A Cuts at: 2344 2638 2344 Size: 294 6997 PmeI GTTT'AAAC Cuts at: 4595 4595 Size: 7291 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2486 2518 4040 4072 5360 5392 6873 6905 2486 Size: 32 1522 32 1288 32 1481 32 2872 Fragments arranged by size: 2872 1522 1481 1288 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2205 2205 Size: 7291 PshAI GACnn'nnGTC Cuts at: 1613 3344 1613 Size: 1731 5560 PsiI TTA'TAA Cuts at: 4861 4861 Size: 7291 PspOMI G'GGCC_C Cuts at: 3250 3538 3250 Size: 288 7003 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2562 2594 2562 Size: 32 7259 PstI C_TGCA'G Cuts at: 830 4643 830 Size: 3813 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7291 PvuII CAG'CTG Cuts at: 2741 3281 3560 4294 2741 Size: 540 279 734 5738 Fragments arranged by size: 5738 734 540 279 SacI G_AGCT'C Cuts at: 3982 4570 3982 Size: 588 6703 SalI G'TCGA_C Cuts at: 1936 4633 1936 Size: 2697 4594 SanDI GG'GwC_CC Cuts at: 2205 2205 Size: 7291 SbfI CC_TGCA'GG Cuts at: 4643 4643 Size: 7291 ScaI AGT'ACT Cuts at: 5539 7251 5539 Size: 1712 5579 SexAI A'CCwGG_T Cuts at: 2958 4169 2958 Size: 1211 6080 SfcI C'TryA_G Cuts at: 826 1045 3111 4639 5774 6455 6646 826 Size: 219 2066 1528 1135 681 191 1471 Fragments arranged by size: 2066 1528 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2035 2134 2035 Size: 99 7192 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7291 SgrAI Cr'CCGG_yG Cuts at: 1700 2030 1700 Size: 330 6961 SmaI CCC'GGG Cuts at: 1872 4618 1872 Size: 2746 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2440 2936 2972 3121 4250 4563 Size: 197 926 497 496 36 149 1129 313 Cuts at: 4563 5395 6296 6558 820 Size: 832 901 262 1553 Fragments arranged by size: 1553 1129 926 901 832 497 496 313 262 197 149 36 SnaBI TAC'GTA Cuts at: 493 5082 493 Size: 4589 2702 SpeI A'CTAG_T Cuts at: 152 3592 3713 152 Size: 3440 121 3730 Fragments arranged by size: 3730 3440 121 SphI G_CATG'C Cuts at: 1132 4649 1132 Size: 3517 3774 SspI AAT'ATT Cuts at: 5 52 5215 5 Size: 47 5163 2081 Fragments arranged by size: 5163 2081 47 StuI AGG'CCT Cuts at: 1517 4086 1517 Size: 2569 4722 StyI C'CwwG_G Cuts at: 513 1065 2076 2261 4391 4730 513 Size: 552 1011 185 2130 339 3074 Fragments arranged by size: 3074 2130 1011 552 339 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3181 5318 5335 5488 5673 6118 325 Size: 1255 1601 2137 17 153 185 445 1498 Fragments arranged by size: 2137 1601 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2483 2596 3074 Size: 274 80 33 51 1443 506 113 478 Cuts at: 3074 5537 7249 96 Size: 2463 1712 138 Fragments arranged by size: 2463 1712 1443 506 478 274 138 113 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2550 2582 2620 2652 7251 7283 2550 Size: 32 38 32 4599 32 2558 Fragments arranged by size: 4599 2558 38 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3059 1316 Size: 1743 5548 XbaI T'CTAG_A Cuts at: 4090 4627 4090 Size: 537 6754 XhoI C'TCGA_G Cuts at: 1943 4563 1943 Size: 2620 4671 XmaI C'CCGG_G Cuts at: 1870 4616 1870 Size: 2746 4545 XmnI GAAnn'nnTTC Cuts at: 3963 5420 3963 Size: 1457 5834 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5099 276 Size: 53 83 186 936 3565 2468 Fragments arranged by size: 3565 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacII SapI SfiI SrfI SwaI XcmI