(Circular) (Six-base) MAPSORT of: pFN21AB6274.seq Check: 4239 from: 1 to: 6796 pFN21AB6274 6796 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4606 278 Size: 53 83 186 936 3070 2468 Fragments arranged by size: 3070 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4139 1937 Size: 2202 4594 Acc65I G'GTAC_C Cuts at: 4117 4117 Size: 6796 AclI AA'CG_TT Cuts at: 1563 4923 5296 1563 Size: 3360 373 3063 Fragments arranged by size: 3360 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2618 2717 3040 3467 4859 6243 6511 1632 Size: 986 99 323 427 1392 1384 268 1917 Fragments arranged by size: 1917 1392 1384 986 427 323 268 99 AfeI AGC'GCT Cuts at: 3011 3963 3011 Size: 952 5844 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6599 AflIII A'CryG_T Cuts at: 1203 3205 5695 6672 1203 Size: 2002 2490 977 1327 Fragments arranged by size: 2490 2002 1327 977 AgeI A'CCGG_T Cuts at: 5627 5627 Size: 6796 AhdI GACnn_n'nnGTC Cuts at: 5525 5525 Size: 6796 AleI CACnn'nnGTG Cuts at: 1166 3194 4028 1166 Size: 2028 834 3934 Fragments arranged by size: 3934 2028 834 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4071 1184 Size: 2887 3909 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3810 3842 3810 Size: 32 6764 AlwNI CAG_nnn'CTG Cuts at: 2157 2194 2267 3809 4082 4539 6111 2157 Size: 37 73 1542 273 457 1572 2842 Fragments arranged by size: 2842 1572 1542 457 273 73 37 ApaI G_GGCC'C Cuts at: 2021 2279 2960 2021 Size: 258 681 5857 Fragments arranged by size: 5857 681 258 ApaLI G'TGCA_C Cuts at: 2378 2417 3831 4046 4853 6009 2378 Size: 39 1414 215 807 1156 3165 Fragments arranged by size: 3165 1414 1156 807 215 39 ApoI r'AATT_y Cuts at: 1493 2240 4105 4331 4483 6592 1493 Size: 747 1865 226 152 2109 1697 Fragments arranged by size: 2109 1865 1697 747 226 152 AseI AT'TA_AT Cuts at: 160 1032 5350 160 Size: 872 4318 1606 Fragments arranged by size: 4318 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6796 AvaI C'yCGr_G Cuts at: 1870 1943 3527 3741 4121 1870 Size: 73 1584 214 380 4545 Fragments arranged by size: 4545 1584 380 214 73 BamHI G'GATC_C Cuts at: 4126 4493 4126 Size: 367 6429 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2481 2809 3955 4117 5572 Size: 325 217 627 694 328 1146 162 1455 Cuts at: 5572 618 Size: 1842 Fragments arranged by size: 1842 1455 1146 694 627 328 325 217 162 BanII G_rGCy'C Cuts at: 1391 2021 2077 2279 2506 2960 3122 3132 3369 Size: 630 56 202 227 454 162 10 237 Cuts at: 3369 3803 4115 1391 Size: 434 312 4072 Fragments arranged by size: 4072 630 454 434 312 237 227 202 162 56 10 BbeI G_GCGC'C Cuts at: 2485 2813 3959 2485 Size: 328 1146 5322 Fragments arranged by size: 5322 1146 328 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2642 3514 928 Size: 971 743 872 4210 Fragments arranged by size: 4210 971 872 743 BbvCI CC'TCA_GC Cuts at: 3474 3474 Size: 6796 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4987 5021 1903 Size: 34 3050 34 3678 Fragments arranged by size: 3678 3050 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4688 5898 4688 Size: 1210 5586 BclI T'GATC_A Cuts at: 1548 1548 Size: 6796 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3912 3921 5407 Size: 107 122 71 631 605 2240 9 1486 Cuts at: 5407 136 Size: 1525 Fragments arranged by size: 2240 1525 1486 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 3144 6791 3144 Size: 3647 3149 BlpI GC'TnA_GC Cuts at: 2723 4213 2723 Size: 1490 5306 Bme1580I G_kGCm'C Cuts at: 1790 2021 2279 2382 2421 2445 2960 3835 4050 Size: 231 258 103 39 24 515 875 215 Cuts at: 4050 4089 4857 6013 1790 Size: 39 768 1156 2573 Fragments arranged by size: 2573 1156 875 768 515 258 231 215 103 39 39 24 BmgBI CAC'GTC Cuts at: 1318 3160 1318 Size: 1842 4954 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2752 3868 4070 Size: 416 53 83 399 24 1335 1116 202 Cuts at: 4070 4527 5485 442 Size: 457 958 1753 Fragments arranged by size: 1753 1335 1116 958 457 416 399 202 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6796 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2513 2885 3957 5456 1239 Size: 1274 372 1072 1499 2579 Fragments arranged by size: 2579 1499 1274 1072 372 Bpu10I CC'TnA_GC Cuts at: 3474 3802 3474 Size: 328 6468 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2789 4921 5786 6084 2789 Size: 2132 865 298 3501 Fragments arranged by size: 3501 2132 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2579 2672 5459 882 Size: 617 1080 93 2787 2219 Fragments arranged by size: 2787 2219 1080 617 93 BsaAI yAC'GTr Cuts at: 493 1204 2527 4030 4587 493 Size: 711 1323 1503 557 2702 Fragments arranged by size: 2702 1503 1323 711 557 BsaBI GATnn'nnATC Cuts at: 6790 6790 Size: 6796 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2482 2790 2810 3037 Size: 53 83 186 936 949 308 20 227 Cuts at: 3037 3956 4603 4985 5620 275 Size: 919 647 382 635 1451 Fragments arranged by size: 1451 949 936 919 647 635 382 308 227 186 83 53 20 BsaWI w'CCGG_w Cuts at: 2748 5229 5627 5901 6048 6459 2748 Size: 2481 398 274 147 411 3085 Fragments arranged by size: 3085 2481 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2342 2372 2738 2768 2840 Size: 30 165 30 939 30 366 30 72 Cuts at: 2840 2870 3677 3707 1178 Size: 30 807 30 4267 Fragments arranged by size: 4267 939 807 366 165 72 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 2861 2888 3698 3701 1186 Size: 1168 507 27 810 3 4281 Fragments arranged by size: 4281 1168 810 507 27 3 BseYI C'CCAG_C Cuts at: 2267 3217 3258 3349 5999 6495 2267 Size: 950 41 91 2650 496 2568 Fragments arranged by size: 2650 2568 950 496 91 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2143 3026 3172 1163 Size: 573 407 883 146 4787 Fragments arranged by size: 4787 883 573 407 146 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4246 5007 6035 1540 Size: 275 186 1240 1005 761 1028 2301 Fragments arranged by size: 2301 1240 1028 1005 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2382 2421 3132 3835 4050 4857 4942 6013 2382 Size: 39 711 703 215 807 85 1071 3165 Fragments arranged by size: 3165 1071 807 711 703 215 85 39 BsmI GAATG_Cn' Cuts at: 4307 4400 4307 Size: 93 6703 BsmBI CGTCTCn'nnnn_ Cuts at: 2797 2797 Size: 6796 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2077 2279 2382 2421 2445 2506 Size: 399 231 56 202 103 39 24 61 Cuts at: 2506 2960 3122 3132 3369 3803 3835 4050 4089 Size: 454 162 10 237 434 32 215 39 Cuts at: 4089 4115 4857 4942 6013 1391 Size: 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 454 434 399 237 231 215 202 162 103 85 61 56 39 39 32 26 24 10 BspEI T'CCGG_A Cuts at: 6459 6459 Size: 6796 BspHI T'CATG_A Cuts at: 4685 6415 4685 Size: 1730 5066 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4151 844 Size: 3307 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2123 2944 3865 4683 1394 Size: 429 300 821 921 818 3507 Fragments arranged by size: 3507 921 821 818 429 300 BsrDI GCAATG_nn' Cuts at: 65 1248 2204 3582 5291 5465 65 Size: 1183 956 1378 1709 174 1396 Fragments arranged by size: 1709 1396 1378 1183 956 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2421 2718 2869 3240 5440 5627 1700 Size: 110 611 297 151 371 2200 187 2869 Fragments arranged by size: 2869 2200 611 371 297 187 151 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6796 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6796 BssSI C'ACGA_G Cuts at: 4856 5868 4856 Size: 1012 5784 BstAPI GCAn_nnn'nTGC Cuts at: 794 2157 2194 2394 3549 794 Size: 1363 37 200 1155 4041 Fragments arranged by size: 4041 1363 1155 200 37 BstBI TT'CG_AA Cuts at: 5600 5600 Size: 6796 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6796 BstXI CCAn_nnnn'nTGG Cuts at: 3599 3599 Size: 6796 BstYI r'GATC_y Cuts at: 1972 2496 2520 3144 3882 4126 4493 4878 4895 Size: 524 24 624 738 244 367 385 17 Cuts at: 4895 6347 6433 6445 6791 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 738 624 524 385 367 346 244 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2476 2814 3750 513 Size: 552 939 472 338 936 3559 Fragments arranged by size: 3559 939 936 552 472 338 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6057 Fragments arranged by size: 6057 683 56 BtsI GCAGTG_nn' Cuts at: 2155 4312 5106 5126 2155 Size: 2157 794 20 3825 Fragments arranged by size: 3825 2157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6796 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6761 DraI TTT'AAA Cuts at: 4100 4100 Size: 6796 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3164 3983 5803 809 Size: 507 1848 819 1820 1802 Fragments arranged by size: 1848 1820 1802 819 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2874 3105 3238 Size: 54 1425 50 275 18 1044 231 133 Cuts at: 3238 3753 3913 3990 4243 5132 6761 8 Size: 515 160 77 253 889 1629 43 Fragments arranged by size: 1629 1425 1044 889 515 275 253 231 160 133 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 4243 1537 Size: 275 1426 1005 4090 Fragments arranged by size: 4090 1426 1005 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2968 3413 4726 1353 Size: 1615 445 1313 3423 Fragments arranged by size: 3423 1615 1313 445 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2303 5383 5757 5903 387 Size: 1064 138 714 3080 374 146 1280 Fragments arranged by size: 3080 1280 1064 714 374 146 138 EcoICRI GAG'CTC Cuts at: 3130 3130 Size: 6796 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2618 2717 2885 3040 3467 3957 Size: 393 881 105 99 168 155 427 490 Cuts at: 3957 4859 5456 6243 6511 1239 Size: 902 597 787 268 1524 Fragments arranged by size: 1524 902 881 787 597 490 427 393 268 168 155 105 99 EcoO109I rG'GnC_Cy Cuts at: 2018 2045 2191 2275 2276 2957 3230 3442 3555 Size: 27 146 84 1 681 273 212 113 Cuts at: 3555 3806 2018 Size: 251 5008 Fragments arranged by size: 5008 681 273 251 212 146 113 84 27 1 EcoRI G'AATT_C Cuts at: 4105 4483 4105 Size: 378 6418 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3031 3031 Size: 6796 FseI GG_CCGG'CC Cuts at: 3244 3244 Size: 6796 FspI TGC'GCA Cuts at: 5302 5302 Size: 6796 HaeII r_GCGC'y Cuts at: 1678 2268 2428 2476 2485 2719 2813 3013 3218 Size: 590 160 48 9 234 94 200 205 Cuts at: 3218 3796 3959 3965 5943 1678 Size: 578 163 6 1978 2531 Fragments arranged by size: 2531 1978 590 578 234 205 200 163 160 94 48 9 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2488 2520 2738 2770 3605 Size: 32 135 32 916 32 218 32 835 Cuts at: 3605 3637 3867 3874 3899 3906 5437 5469 5511 Size: 32 230 7 25 7 1531 32 42 Cuts at: 5511 5543 1373 Size: 32 2626 Fragments arranged by size: 2626 1531 916 835 230 218 135 42 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 4140 4386 669 Size: 988 281 2202 246 3079 Fragments arranged by size: 3079 2202 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6796 HpaI GTT'AAC Cuts at: 4386 4386 Size: 6796 KasI G'GCGC_C Cuts at: 2481 2809 3955 2481 Size: 328 1146 5322 Fragments arranged by size: 5322 1146 328 KpnI G_GTAC'C Cuts at: 4121 4121 Size: 6796 MfeI C'AATT_G Cuts at: 4395 4395 Size: 6796 MluI A'CGCG_T Cuts at: 6672 6672 Size: 6796 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3075 5910 6094 1305 Size: 1770 2835 184 2007 Fragments arranged by size: 2835 2007 1770 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3755 3992 10 Size: 54 1425 343 1923 237 2814 Fragments arranged by size: 2814 1923 1425 343 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 3194 4028 4053 4754 5113 5272 518 Size: 648 2028 834 25 701 359 159 2042 Fragments arranged by size: 2042 2028 834 701 648 359 159 25 MspA1I CmG'CkG Cuts at: 1606 2478 2491 3349 3849 3921 4212 4889 6037 Size: 872 13 858 500 72 291 677 1148 Cuts at: 6037 6282 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 872 858 677 500 291 245 72 13 NaeI GCC'GGC Cuts at: 2720 3242 2720 Size: 522 6274 NarI GG'CG_CC Cuts at: 2482 2810 3956 2482 Size: 328 1146 5322 Fragments arranged by size: 5322 1146 328 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5305 Fragments arranged by size: 5305 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6796 NgoMIV G'CCGG_C Cuts at: 2718 3240 2718 Size: 522 6274 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6796 NotI GC'GGCC_GC Cuts at: 4243 4243 Size: 6796 NspI r_CATG'y Cuts at: 1132 1229 3508 4154 5699 1132 Size: 97 2279 646 1545 2229 Fragments arranged by size: 2279 2229 1545 646 97 PasI CC'CwG_GG Cuts at: 1348 3785 1348 Size: 2437 4359 PciI A'CATG_T Cuts at: 5695 5695 Size: 6796 PflMI CCAn_nnn'nTGG Cuts at: 1108 2267 1108 Size: 1159 5637 PfoI T'CCnGG_A Cuts at: 3031 3168 3031 Size: 137 6659 PmeI GTTT'AAAC Cuts at: 4100 4100 Size: 6796 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4865 4897 6378 6410 4865 Size: 32 1481 32 5251 Fragments arranged by size: 5251 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2045 3230 3442 3555 2045 Size: 1185 212 113 5286 Fragments arranged by size: 5286 1185 212 113 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6796 PsiI TTA'TAA Cuts at: 4366 4366 Size: 6796 PspOMI G'GGCC_C Cuts at: 2017 2275 2956 2017 Size: 258 681 5857 Fragments arranged by size: 5857 681 258 PstI C_TGCA'G Cuts at: 830 2153 2213 2490 2894 4148 830 Size: 1323 60 277 404 1254 3478 Fragments arranged by size: 3478 1323 1254 404 277 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6796 PvuII CAG'CTG Cuts at: 2491 3349 2491 Size: 858 5938 RsrII CG'GwC_CG Cuts at: 3286 3286 Size: 6796 SacI G_AGCT'C Cuts at: 3132 3132 Size: 6796 SacII CC_GC'GG Cuts at: 2479 2479 Size: 6796 SalI G'TCGA_C Cuts at: 1936 4138 1936 Size: 2202 4594 SanDI GG'GwC_CC Cuts at: 2045 3555 2045 Size: 1510 5286 SbfI CC_TGCA'GG Cuts at: 4148 4148 Size: 6796 ScaI AGT'ACT Cuts at: 5044 6756 5044 Size: 1712 5084 SfcI C'TryA_G Cuts at: 826 1045 2149 2209 2326 2486 2890 3436 4144 Size: 219 1104 60 117 160 404 546 708 Cuts at: 4144 5279 5960 6151 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1104 708 681 546 404 219 191 160 117 60 SfoI GGC'GCC Cuts at: 2483 2811 3957 2483 Size: 328 1146 5322 Fragments arranged by size: 5322 1146 328 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6796 SgrAI Cr'CCGG_yG Cuts at: 1700 2421 1700 Size: 721 6075 SmaI CCC'GGG Cuts at: 1872 3529 3743 4123 1872 Size: 1657 214 380 4545 Fragments arranged by size: 4545 1657 380 214 SmlI C'TyrA_G Cuts at: 820 1017 1943 2804 4900 5801 6063 820 Size: 197 926 861 2096 901 262 1553 Fragments arranged by size: 2096 1553 926 901 861 262 197 SnaBI TAC'GTA Cuts at: 493 4587 493 Size: 4094 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6796 SphI G_CATG'C Cuts at: 1132 4154 1132 Size: 3022 3774 SspI AAT'ATT Cuts at: 5 52 4720 5 Size: 47 4668 2081 Fragments arranged by size: 4668 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6796 StyI C'CwwG_G Cuts at: 513 1065 2004 4235 513 Size: 552 939 2231 3074 Fragments arranged by size: 3074 2231 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3272 3798 4823 4840 4993 5178 5623 Size: 1255 1692 526 1025 17 153 185 445 Cuts at: 5623 325 Size: 1498 Fragments arranged by size: 1692 1498 1255 1025 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5042 6754 96 Size: 274 80 33 51 1443 3065 1712 138 Fragments arranged by size: 3065 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2342 2374 6756 6788 2342 Size: 32 4382 32 2350 Fragments arranged by size: 4382 2350 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6796 XbaI T'CTAG_A Cuts at: 4132 4132 Size: 6796 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2531 2011 Size: 520 6276 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6796 XmaI C'CCGG_G Cuts at: 1870 3527 3741 4121 1870 Size: 1657 214 380 4545 Fragments arranged by size: 4545 1657 380 214 XmnI GAAnn'nnTTC Cuts at: 3521 4925 3521 Size: 1404 5392 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4604 276 Size: 53 83 186 936 3070 2468 Fragments arranged by size: 3070 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI SapI SexAI SfiI SrfI SwaI