(Circular) (Six-base) MAPSORT of: pFN21AB6241.seq Check: 3635 from: 1 to: 6958 pFN21AB6241 6958 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4768 278 Size: 53 83 186 936 3232 2468 Fragments arranged by size: 3232 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3264 4301 1937 Size: 1327 1037 4594 Fragments arranged by size: 4594 1327 1037 Acc65I G'GTAC_C Cuts at: 4279 4279 Size: 6958 AclI AA'CG_TT Cuts at: 1563 3547 5085 5458 1563 Size: 1984 1538 373 3063 Fragments arranged by size: 3063 1984 1538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 2554 2722 3982 5021 6405 6673 1632 Size: 747 175 168 1260 1039 1384 268 1917 Fragments arranged by size: 1917 1384 1260 1039 747 268 175 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6761 AflIII A'CryG_T Cuts at: 1203 2269 5857 6834 1203 Size: 1066 3588 977 1327 Fragments arranged by size: 3588 1327 1066 977 AgeI A'CCGG_T Cuts at: 5789 5789 Size: 6958 AhdI GACnn_n'nnGTC Cuts at: 5687 5687 Size: 6958 AleI CACnn'nnGTG Cuts at: 1166 3537 4212 1166 Size: 2371 675 3912 Fragments arranged by size: 3912 2371 675 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6958 AlwNI CAG_nnn'CTG Cuts at: 4701 6273 4701 Size: 1572 5386 ApaI G_GGCC'C Cuts at: 2110 2110 Size: 6958 ApaLI G'TGCA_C Cuts at: 3014 5015 6171 3014 Size: 2001 1156 3801 Fragments arranged by size: 3801 2001 1156 ApoI r'AATT_y Cuts at: 1493 2160 2524 4267 4493 4645 6754 1493 Size: 667 364 1743 226 152 2109 1697 Fragments arranged by size: 2109 1743 1697 667 364 226 152 AseI AT'TA_AT Cuts at: 160 1032 5512 160 Size: 872 4480 1606 Fragments arranged by size: 4480 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 AvaI C'yCGr_G Cuts at: 1870 1943 3703 4283 1870 Size: 73 1760 580 4545 Fragments arranged by size: 4545 1760 580 73 BamHI G'GATC_C Cuts at: 4288 4655 4288 Size: 367 6591 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4279 5734 618 Size: 325 217 627 2492 1455 1842 Fragments arranged by size: 2492 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2096 2110 4277 1391 Size: 705 14 2167 4072 Fragments arranged by size: 4072 2167 705 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2236 3785 928 Size: 971 337 1549 4101 Fragments arranged by size: 4101 1549 971 337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5149 5183 1903 Size: 34 3212 34 3678 Fragments arranged by size: 3678 3212 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4029 4850 6060 4029 Size: 821 1210 4927 Fragments arranged by size: 4927 1210 821 BclI T'GATC_A Cuts at: 1548 2332 1548 Size: 784 6174 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2241 5569 136 Size: 107 122 71 631 605 569 3328 1525 Fragments arranged by size: 3328 1525 631 605 569 122 107 71 BglII A'GATC_T Cuts at: 6953 6953 Size: 6958 BlpI GC'TnA_GC Cuts at: 2090 4375 2090 Size: 2285 4673 Bme1580I G_kGCm'C Cuts at: 1790 2110 2214 3018 3532 5019 6175 1790 Size: 320 104 804 514 1487 1156 2573 Fragments arranged by size: 2573 1487 1156 804 514 320 104 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6958 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4689 5647 442 Size: 416 53 83 399 24 3272 958 1753 Fragments arranged by size: 3272 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6958 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2115 2115 Size: 6958 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 5618 1239 Size: 911 3468 2579 Fragments arranged by size: 3468 2579 911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2416 2729 5083 5948 6246 2416 Size: 313 2354 865 298 3128 Fragments arranged by size: 3128 2354 865 313 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5621 882 Size: 617 4122 2219 Fragments arranged by size: 4122 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4214 4749 493 Size: 711 3010 535 2702 Fragments arranged by size: 3010 2702 711 535 BsaBI GATnn'nnATC Cuts at: 2588 6952 2588 Size: 4364 2594 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2233 4765 5147 5782 Size: 53 83 186 936 700 2532 382 635 Cuts at: 5782 275 Size: 1451 Fragments arranged by size: 2532 1451 936 700 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5391 5789 6063 6210 6621 5391 Size: 398 274 147 411 5728 Fragments arranged by size: 5728 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2431 2461 4228 4258 1178 Size: 30 165 30 1028 30 1767 30 3878 Fragments arranged by size: 3878 1767 1028 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2452 2747 3167 3386 3470 3755 3923 1186 Size: 1266 295 420 219 84 285 168 4221 Fragments arranged by size: 4221 1266 420 295 285 219 168 84 BseYI C'CCAG_C Cuts at: 6161 6657 6161 Size: 496 6462 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2271 2571 2737 3157 1163 Size: 573 535 300 166 420 4964 Fragments arranged by size: 4964 573 535 420 300 166 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4408 5169 6197 1540 Size: 275 186 2407 761 1028 2301 Fragments arranged by size: 2407 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3018 5019 5104 6175 3018 Size: 2001 85 1071 3801 Fragments arranged by size: 3801 2001 1071 85 BsmI GAATG_Cn' Cuts at: 2540 2888 2944 4151 4469 4562 2540 Size: 348 56 1207 318 93 4936 Fragments arranged by size: 4936 1207 348 318 93 56 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2110 2214 3018 3532 4277 5019 Size: 399 306 14 104 804 514 745 742 Cuts at: 5019 5104 6175 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 804 745 742 514 399 306 104 85 14 BspEI T'CCGG_A Cuts at: 6621 6621 Size: 6958 BspHI T'CATG_A Cuts at: 4847 6577 4847 Size: 1730 5228 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4313 844 Size: 3469 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4845 1394 Size: 429 3022 3507 Fragments arranged by size: 3507 3022 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2669 5453 5627 65 Size: 1183 1421 2784 174 1396 Fragments arranged by size: 2784 1421 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 5602 5789 1700 Size: 110 425 3367 187 2869 Fragments arranged by size: 3367 2869 425 187 110 BsrGI T'GTAC_A Cuts at: 96 2112 4229 96 Size: 2016 2117 2825 Fragments arranged by size: 2825 2117 2016 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6958 BssSI C'ACGA_G Cuts at: 5018 6030 5018 Size: 1012 5946 BstAPI GCAn_nnn'nTGC Cuts at: 794 3732 794 Size: 2938 4020 BstBI TT'CG_AA Cuts at: 5762 5762 Size: 6958 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6958 BstXI CCAn_nnnn'nTGG Cuts at: 2894 3062 4157 2894 Size: 168 1095 5695 Fragments arranged by size: 5695 1095 168 BstYI r'GATC_y Cuts at: 1972 4288 4655 5040 5057 6509 6595 6607 6953 Size: 2316 367 385 17 1452 86 12 346 Cuts at: 6953 1972 Size: 1977 Fragments arranged by size: 2316 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2013 3524 2013 Size: 1511 5447 BtgI C'CryG_G Cuts at: 513 1065 2004 2391 513 Size: 552 939 387 5080 Fragments arranged by size: 5080 939 552 387 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6219 Fragments arranged by size: 6219 683 56 BtsI GCAGTG_nn' Cuts at: 2725 3149 3653 4474 5268 5288 2725 Size: 424 504 821 794 20 4395 Fragments arranged by size: 4395 821 794 504 424 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6958 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2791 2826 2959 2994 3379 3414 3883 Size: 35 2227 35 133 35 385 35 469 Cuts at: 3883 3918 529 Size: 35 3569 Fragments arranged by size: 3569 2227 469 385 133 35 35 35 35 35 DraI TTT'AAA Cuts at: 4262 4262 Size: 6958 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5965 809 Size: 507 4649 1802 Fragments arranged by size: 4649 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2237 3064 4405 Size: 54 1425 50 275 18 407 827 1341 Cuts at: 4405 5294 6923 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1341 889 827 407 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4405 1537 Size: 275 2593 4090 Fragments arranged by size: 4090 2593 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2217 3773 4001 4888 1353 Size: 864 1556 228 887 3423 Fragments arranged by size: 3423 1556 887 864 228 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4048 5545 5919 6065 387 Size: 1064 138 2459 1497 374 146 1280 Fragments arranged by size: 2459 1497 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2379 2554 2722 3982 5021 5618 Size: 393 518 229 175 168 1260 1039 597 Cuts at: 5618 6405 6673 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1260 1039 787 597 518 393 268 229 175 168 EcoO109I rG'GnC_Cy Cuts at: 2009 2106 2107 2243 2009 Size: 97 1 136 6724 Fragments arranged by size: 6724 136 97 1 EcoRI G'AATT_C Cuts at: 2160 4267 4645 2160 Size: 2107 378 4473 Fragments arranged by size: 4473 2107 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3022 3442 4225 3022 Size: 420 783 5755 Fragments arranged by size: 5755 783 420 FspI TGC'GCA Cuts at: 5464 5464 Size: 6958 HaeII r_GCGC'y Cuts at: 1678 6105 1678 Size: 4427 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2470 2502 2903 2935 3396 Size: 32 135 32 898 32 401 32 461 Cuts at: 3396 3428 3491 3523 3732 3764 4238 4270 5599 Size: 32 63 32 209 32 474 32 1329 Cuts at: 5599 5631 5673 5705 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1329 898 474 461 401 209 135 63 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3339 3423 4302 4548 669 Size: 988 281 1317 84 84 879 246 3079 Fragments arranged by size: 3079 1317 988 879 281 246 84 84 HindIII A'AGCT_T Cuts at: 748 2338 3238 748 Size: 1590 900 4468 Fragments arranged by size: 4468 1590 900 HpaI GTT'AAC Cuts at: 4548 4548 Size: 6958 KpnI G_GTAC'C Cuts at: 4283 4283 Size: 6958 MfeI C'AATT_G Cuts at: 4557 4557 Size: 6958 MluI A'CGCG_T Cuts at: 6834 6834 Size: 6958 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6072 6256 1305 Size: 4767 184 2007 Fragments arranged by size: 4767 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5136 Fragments arranged by size: 5136 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2545 3537 4212 4916 5275 5434 518 Size: 648 1379 992 675 704 359 159 2042 Fragments arranged by size: 2042 1379 992 704 675 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2357 4002 4374 5051 6199 6444 1606 Size: 751 1645 372 677 1148 245 2120 Fragments arranged by size: 2120 1645 1148 751 677 372 245 NaeI GCC'GGC Cuts at: 2237 2237 Size: 6958 NcoI C'CATG_G Cuts at: 513 1065 2004 2391 513 Size: 552 939 387 5080 Fragments arranged by size: 5080 939 552 387 NdeI CA'TA_TG Cuts at: 387 387 Size: 6958 NgoMIV G'CCGG_C Cuts at: 2235 2235 Size: 6958 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6958 NotI GC'GGCC_GC Cuts at: 4405 4405 Size: 6958 NspI r_CATG'y Cuts at: 1132 1229 2273 2423 2634 4316 5861 1132 Size: 97 1044 150 211 1682 1545 2229 Fragments arranged by size: 2229 1682 1545 1044 211 150 97 PasI CC'CwG_GG Cuts at: 1348 2214 1348 Size: 866 6092 PciI A'CATG_T Cuts at: 2269 5857 2269 Size: 3588 3370 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6958 PmeI GTTT'AAAC Cuts at: 4262 4262 Size: 6958 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5027 5059 6540 6572 5027 Size: 32 1481 32 5413 Fragments arranged by size: 5413 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2009 2009 Size: 6958 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6958 PsiI TTA'TAA Cuts at: 4528 4528 Size: 6958 PspOMI G'GGCC_C Cuts at: 2106 2106 Size: 6958 PstI C_TGCA'G Cuts at: 830 3560 4310 830 Size: 2730 750 3478 Fragments arranged by size: 3478 2730 750 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6958 PvuII CAG'CTG Cuts at: 2357 4002 2357 Size: 1645 5313 SalI G'TCGA_C Cuts at: 1936 4300 1936 Size: 2364 4594 SbfI CC_TGCA'GG Cuts at: 4310 4310 Size: 6958 ScaI AGT'ACT Cuts at: 5206 6918 5206 Size: 1712 5246 SfcI C'TryA_G Cuts at: 826 1045 2137 2490 3556 3808 4186 4306 5441 Size: 219 1092 353 1066 252 378 120 1135 Cuts at: 5441 6122 6313 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1092 1066 681 378 353 252 219 191 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6958 SmaI CCC'GGG Cuts at: 1872 4285 1872 Size: 2413 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2431 2744 5062 5963 6225 820 Size: 197 926 488 313 2318 901 262 1553 Fragments arranged by size: 2318 1553 926 901 488 313 262 197 SnaBI TAC'GTA Cuts at: 493 4749 493 Size: 4256 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6958 SphI G_CATG'C Cuts at: 1132 4316 1132 Size: 3184 3774 SspI AAT'ATT Cuts at: 5 52 4882 5 Size: 47 4830 2081 Fragments arranged by size: 4830 2081 47 StuI AGG'CCT Cuts at: 1517 4138 1517 Size: 2621 4337 StyI C'CwwG_G Cuts at: 513 1065 2004 2391 4397 513 Size: 552 939 387 2006 3074 Fragments arranged by size: 3074 2006 939 552 387 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 3527 4232 4985 5002 5155 5340 Size: 1255 504 1443 705 753 17 153 185 Cuts at: 5340 5785 325 Size: 445 1498 Fragments arranged by size: 1498 1443 1255 753 705 504 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 4229 5204 Size: 274 80 33 51 1443 135 2117 975 Cuts at: 5204 6916 96 Size: 1712 138 Fragments arranged by size: 2117 1712 1443 975 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2431 2463 4228 4260 6918 6950 2431 Size: 32 1765 32 2658 32 2439 Fragments arranged by size: 2658 2439 1765 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6958 XbaI T'CTAG_A Cuts at: 4294 4294 Size: 6958 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6958 XmaI C'CCGG_G Cuts at: 1870 4283 1870 Size: 2413 4545 XmnI GAAnn'nnTTC Cuts at: 3632 3800 3968 5087 3632 Size: 168 168 1119 5503 Fragments arranged by size: 5503 1119 168 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4766 276 Size: 53 83 186 936 3232 2468 Fragments arranged by size: 3232 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI