(Circular) (Six-base) MAPSORT of: pFN21AB6240.seq Check: 5605 from: 1 to: 6736 pFN21AB6240 6736 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2512 3117 2512 Size: 605 6131 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4546 278 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3122 3748 4079 1937 Size: 1185 626 331 4594 Fragments arranged by size: 4594 1185 626 331 Acc65I G'GTAC_C Cuts at: 4057 4057 Size: 6736 AclI AA'CG_TT Cuts at: 1563 3588 4863 5236 1563 Size: 2025 1275 373 3063 Fragments arranged by size: 3063 2025 1275 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 2198 2267 3960 4799 6183 6451 1632 Size: 542 24 69 1693 839 1384 268 1917 Fragments arranged by size: 1917 1693 1384 839 542 268 69 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6539 AflIII A'CryG_T Cuts at: 1203 2774 3904 5635 6612 1203 Size: 1571 1130 1731 977 1327 Fragments arranged by size: 1731 1571 1327 1130 977 AgeI A'CCGG_T Cuts at: 5567 5567 Size: 6736 AhdI GACnn_n'nnGTC Cuts at: 5465 5465 Size: 6736 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6736 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6736 AlwNI CAG_nnn'CTG Cuts at: 4479 6051 4479 Size: 1572 5164 ApaI G_GGCC'C Cuts at: 3948 3948 Size: 6736 ApaLI G'TGCA_C Cuts at: 4793 5949 4793 Size: 1156 5580 ApoI r'AATT_y Cuts at: 1493 3374 3623 4045 4271 4423 6532 1493 Size: 1881 249 422 226 152 2109 1697 Fragments arranged by size: 2109 1881 1697 422 249 226 152 AseI AT'TA_AT Cuts at: 160 1032 5290 160 Size: 872 4258 1606 Fragments arranged by size: 4258 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 AvaI C'yCGr_G Cuts at: 1870 1943 2630 4061 1870 Size: 73 687 1431 4545 Fragments arranged by size: 4545 1431 687 73 BamHI G'GATC_C Cuts at: 4066 4433 4066 Size: 367 6369 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4057 5512 618 Size: 325 217 627 2270 1455 1842 Fragments arranged by size: 2270 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2372 3921 3948 4055 1391 Size: 981 1549 27 107 4072 Fragments arranged by size: 4072 1549 981 107 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2090 3551 928 Size: 971 191 1461 4113 Fragments arranged by size: 4113 1461 971 191 BbvCI CC'TCA_GC Cuts at: 2373 3144 2373 Size: 771 5965 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4927 4961 1903 Size: 34 2990 34 3678 Fragments arranged by size: 3678 2990 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4628 5838 4628 Size: 1210 5526 BclI T'GATC_A Cuts at: 1548 1548 Size: 6736 BfrBI ATG'CAT Cuts at: 3416 3416 Size: 6736 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5347 136 Size: 107 122 71 631 605 3675 1525 Fragments arranged by size: 3675 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3474 3875 6731 3474 Size: 401 2856 3479 Fragments arranged by size: 3479 2856 401 BlpI GC'TnA_GC Cuts at: 2366 4153 2366 Size: 1787 4949 Bme1580I G_kGCm'C Cuts at: 1790 3342 3948 4797 5953 1790 Size: 1552 606 849 1156 2573 Fragments arranged by size: 2573 1552 1156 849 606 BmgBI CAC'GTC Cuts at: 1318 3907 1318 Size: 2589 4147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4467 5425 442 Size: 416 53 83 399 24 3050 958 1753 Fragments arranged by size: 3050 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6736 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3231 3231 Size: 6736 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2366 2378 3432 5396 1239 Size: 1097 30 12 1054 1964 2579 Fragments arranged by size: 2579 1964 1097 1054 30 12 Bpu10I CC'TnA_GC Cuts at: 2373 3144 2373 Size: 771 5965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2301 2649 4861 5726 6024 2301 Size: 348 2212 865 298 3013 Fragments arranged by size: 3013 2212 865 348 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5399 882 Size: 617 3900 2219 Fragments arranged by size: 3900 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3528 4527 493 Size: 711 2324 999 2702 Fragments arranged by size: 2702 2324 999 711 BsaBI GATnn'nnATC Cuts at: 2248 6730 2248 Size: 4482 2254 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2126 4543 4925 5560 Size: 53 83 186 936 593 2417 382 635 Cuts at: 5560 275 Size: 1451 Fragments arranged by size: 2417 1451 936 635 593 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5169 5567 5841 5988 6399 5169 Size: 398 274 147 411 5506 Fragments arranged by size: 5506 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 1178 Size: 30 165 30 693 30 5788 Fragments arranged by size: 5788 693 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2381 2773 2776 2900 1186 Size: 1195 392 3 124 5022 Fragments arranged by size: 5022 1195 392 124 3 BseYI C'CCAG_C Cuts at: 2166 2331 2532 5939 6435 2166 Size: 165 201 3407 496 2467 Fragments arranged by size: 3407 2467 496 201 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2775 3685 1163 Size: 573 1039 910 4214 Fragments arranged by size: 4214 1039 910 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2133 4186 4947 5975 1540 Size: 275 186 132 2053 761 1028 2301 Fragments arranged by size: 2301 2053 1028 761 275 186 132 BsiHKAI G_wGCw'C Cuts at: 2719 2892 4797 4882 5953 2719 Size: 173 1905 85 1071 3502 Fragments arranged by size: 3502 1905 1071 173 85 BsmI GAATG_Cn' Cuts at: 3541 4247 4340 3541 Size: 706 93 5937 Fragments arranged by size: 5937 706 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2117 2117 Size: 6736 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2372 2719 2892 3342 3921 3948 4055 Size: 399 582 347 173 450 579 27 107 Cuts at: 4055 4797 4882 5953 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 582 579 450 399 347 173 107 85 27 BspEI T'CCGG_A Cuts at: 6399 6399 Size: 6736 BspHI T'CATG_A Cuts at: 4625 6355 4625 Size: 1730 5006 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2512 3031 3117 4091 844 Size: 1668 519 86 974 3489 Fragments arranged by size: 3489 1668 974 519 86 BsrBI CCG'CTC Cuts at: 1394 1823 4623 1394 Size: 429 2800 3507 Fragments arranged by size: 3507 2800 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3837 5231 5405 65 Size: 1183 2589 1394 174 1396 Fragments arranged by size: 2589 1396 1394 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2128 5380 5567 1700 Size: 110 318 3252 187 2869 Fragments arranged by size: 3252 2869 318 187 110 BsrGI T'GTAC_A Cuts at: 96 3046 3901 96 Size: 2950 855 2931 Fragments arranged by size: 2950 2931 855 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6736 BssSI C'ACGA_G Cuts at: 4796 5808 4796 Size: 1012 5724 BstAPI GCAn_nnn'nTGC Cuts at: 794 2362 3359 3892 794 Size: 1568 997 533 3638 Fragments arranged by size: 3638 1568 997 533 BstBI TT'CG_AA Cuts at: 5540 5540 Size: 6736 BstEII G'GTnAC_C Cuts at: 1184 3656 1184 Size: 2472 4264 BstYI r'GATC_y Cuts at: 1972 2346 2761 3474 3875 4066 4433 4818 4835 Size: 374 415 713 401 191 367 385 17 Cuts at: 4835 6287 6373 6385 6731 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 713 415 401 385 374 367 346 191 86 17 12 BstZ17I GTA'TAC Cuts at: 3749 3749 Size: 6736 BtgI C'CryG_G Cuts at: 513 1065 2133 3771 513 Size: 552 1068 1638 3478 Fragments arranged by size: 3478 1638 1068 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5997 Fragments arranged by size: 5997 683 56 BtsI GCAGTG_nn' Cuts at: 2955 3546 4252 5046 5066 2955 Size: 591 706 794 20 4625 Fragments arranged by size: 4625 794 706 591 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6736 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6701 DraI TTT'AAA Cuts at: 4040 4040 Size: 6736 DraIII CAC_nnn'GTG Cuts at: 3298 3298 Size: 6736 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5743 809 Size: 507 4427 1802 Fragments arranged by size: 4427 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2933 3522 Size: 54 1425 50 275 18 300 803 589 Cuts at: 3522 4183 5072 6701 8 Size: 661 889 1629 43 Fragments arranged by size: 1629 1425 889 803 661 589 300 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2130 4183 1537 Size: 275 318 2053 4090 Fragments arranged by size: 4090 2053 318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 3810 3909 4666 1353 Size: 933 1524 99 757 3423 Fragments arranged by size: 3423 1524 933 757 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5323 5697 5843 387 Size: 1064 138 3734 374 146 1280 Fragments arranged by size: 3734 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2198 2267 2336 2366 2378 3432 Size: 393 542 24 69 69 30 12 1054 Cuts at: 3432 3960 4799 5396 6183 6451 1239 Size: 528 839 597 787 268 1524 Fragments arranged by size: 1524 1054 839 787 597 542 528 393 268 69 69 30 24 12 EcoO109I rG'GnC_Cy Cuts at: 2407 3263 3302 3944 3945 2407 Size: 856 39 642 1 5198 Fragments arranged by size: 5198 856 642 39 1 EcoRI G'AATT_C Cuts at: 3623 4045 4423 3623 Size: 422 378 5936 Fragments arranged by size: 5936 422 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2824 2824 Size: 6736 FspI TGC'GCA Cuts at: 3363 5242 3363 Size: 1879 4857 HaeII r_GCGC'y Cuts at: 1678 2608 5883 1678 Size: 930 3275 2531 Fragments arranged by size: 3275 2531 930 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2299 2331 2701 Size: 32 135 32 524 32 171 32 370 Cuts at: 2701 2733 2951 2983 5377 5409 5451 5483 1373 Size: 32 218 32 2394 32 42 32 2626 Fragments arranged by size: 2626 2394 524 370 218 171 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4080 4326 669 Size: 988 281 2142 246 3079 Fragments arranged by size: 3079 2142 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6736 HpaI GTT'AAC Cuts at: 4326 4326 Size: 6736 KpnI G_GTAC'C Cuts at: 4061 4061 Size: 6736 MfeI C'AATT_G Cuts at: 4335 4335 Size: 6736 MluI A'CGCG_T Cuts at: 6612 6612 Size: 6736 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 2702 3248 3535 5850 6034 1305 Size: 834 563 546 287 2315 184 2007 Fragments arranged by size: 2315 2007 834 563 546 287 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2935 10 Size: 54 1425 343 1103 3811 Fragments arranged by size: 3811 1425 1103 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3111 3287 3494 4694 5053 5212 518 Size: 648 1945 176 207 1200 359 159 2042 Fragments arranged by size: 2042 1945 1200 648 359 207 176 159 MspA1I CmG'CkG Cuts at: 1606 2150 4152 4829 5977 6222 1606 Size: 544 2002 677 1148 245 2120 Fragments arranged by size: 2120 2002 1148 677 544 245 NaeI GCC'GGC Cuts at: 2130 2130 Size: 6736 NcoI C'CATG_G Cuts at: 513 1065 3771 513 Size: 552 2706 3478 Fragments arranged by size: 3478 2706 552 NdeI CA'TA_TG Cuts at: 387 3412 387 Size: 3025 3711 NgoMIV G'CCGG_C Cuts at: 2128 2128 Size: 6736 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6736 NotI GC'GGCC_GC Cuts at: 4183 4183 Size: 6736 NsiI A_TGCA'T Cuts at: 3418 3418 Size: 6736 NspI r_CATG'y Cuts at: 1132 1229 2495 2778 2828 2999 3116 3292 3682 Size: 97 1266 283 50 171 117 176 390 Cuts at: 3682 4094 5639 1132 Size: 412 1545 2229 Fragments arranged by size: 2229 1545 1266 412 390 283 176 171 117 97 50 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6736 PciI A'CATG_T Cuts at: 2774 5635 2774 Size: 2861 3875 PflMI CCAn_nnn'nTGG Cuts at: 1108 2539 1108 Size: 1431 5305 PmeI GTTT'AAAC Cuts at: 4040 4040 Size: 6736 PmlI CAC'GTG Cuts at: 3528 3528 Size: 6736 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4805 4837 6318 6350 4805 Size: 32 1481 32 5191 Fragments arranged by size: 5191 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3302 3302 Size: 6736 PshAI GACnn'nnGTC Cuts at: 1613 2777 3251 3864 1613 Size: 1164 474 613 4485 Fragments arranged by size: 4485 1164 613 474 PsiI TTA'TAA Cuts at: 2445 3381 4306 2445 Size: 936 925 4875 Fragments arranged by size: 4875 936 925 PspOMI G'GGCC_C Cuts at: 3944 3944 Size: 6736 PstI C_TGCA'G Cuts at: 830 2691 2728 4088 830 Size: 1861 37 1360 3478 Fragments arranged by size: 3478 1861 1360 37 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6736 PvuII CAG'CTG Cuts at: 2150 2150 Size: 6736 SalI G'TCGA_C Cuts at: 1936 4078 1936 Size: 2142 4594 SanDI GG'GwC_CC Cuts at: 3302 3302 Size: 6736 SapI GCTCTTCn'nnn_ Cuts at: 2286 3810 3909 2286 Size: 1524 99 5113 Fragments arranged by size: 5113 1524 99 SbfI CC_TGCA'GG Cuts at: 4088 4088 Size: 6736 ScaI AGT'ACT Cuts at: 4984 6696 4984 Size: 1712 5024 SfcI C'TryA_G Cuts at: 826 1045 2687 2724 3062 3123 4084 5219 5900 Size: 219 1642 37 338 61 961 1135 681 Cuts at: 5900 6091 826 Size: 191 1471 Fragments arranged by size: 1642 1471 1135 961 681 338 219 191 61 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6736 SmaI CCC'GGG Cuts at: 1872 2632 4063 1872 Size: 760 1431 4545 Fragments arranged by size: 4545 1431 760 SmlI C'TyrA_G Cuts at: 820 1017 1943 2316 2664 4840 5741 6003 820 Size: 197 926 373 348 2176 901 262 1553 Fragments arranged by size: 2176 1553 926 901 373 348 262 197 SnaBI TAC'GTA Cuts at: 493 4527 493 Size: 4034 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6736 SphI G_CATG'C Cuts at: 1132 2999 3292 4094 1132 Size: 1867 293 802 3774 Fragments arranged by size: 3774 1867 802 293 SrfI GCCC'GGGC Cuts at: 2632 2632 Size: 6736 SspI AAT'ATT Cuts at: 5 52 4660 5 Size: 47 4608 2081 Fragments arranged by size: 4608 2081 47 StuI AGG'CCT Cuts at: 1517 2613 1517 Size: 1096 5640 StyI C'CwwG_G Cuts at: 513 1065 2608 3259 3771 4175 513 Size: 552 1543 651 512 404 3074 Fragments arranged by size: 3074 1543 651 552 512 404 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3272 4763 4780 4933 5118 5563 325 Size: 1255 1692 1491 17 153 185 445 1498 Fragments arranged by size: 1692 1498 1491 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3014 3031 3046 Size: 274 80 33 51 1443 1037 17 15 Cuts at: 3046 3901 4982 6694 96 Size: 855 1081 1712 138 Fragments arranged by size: 1712 1443 1081 1037 855 274 138 80 51 33 17 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6696 6728 6696 Size: 32 6704 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6736 XbaI T'CTAG_A Cuts at: 4072 4072 Size: 6736 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2909 2909 Size: 6736 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6736 XmaI C'CCGG_G Cuts at: 1870 2630 4061 1870 Size: 760 1431 4545 Fragments arranged by size: 4545 1431 760 XmnI GAAnn'nnTTC Cuts at: 4865 4865 Size: 6736 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4544 276 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstXI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SwaI