(Circular) (Six-base) MAPSORT of: pFN21AB6234.seq Check: 2596 from: 1 to: 6736 pFN21AB6234 6736 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2775 2775 Size: 6736 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4546 278 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4079 1937 Size: 2142 4594 Acc65I G'GTAC_C Cuts at: 4057 4057 Size: 6736 AclI AA'CG_TT Cuts at: 1563 4863 5236 1563 Size: 3300 373 3063 Fragments arranged by size: 3300 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2673 2815 3323 3375 4799 6183 6451 1632 Size: 1041 142 508 52 1424 1384 268 1917 Fragments arranged by size: 1917 1424 1384 1041 508 268 142 52 AfeI AGC'GCT Cuts at: 3415 3415 Size: 6736 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6539 AflIII A'CryG_T Cuts at: 1203 2388 3055 5635 6612 1203 Size: 1185 667 2580 977 1327 Fragments arranged by size: 2580 1327 1185 977 667 AgeI A'CCGG_T Cuts at: 5567 5567 Size: 6736 AhdI GACnn_n'nnGTC Cuts at: 5465 5465 Size: 6736 AleI CACnn'nnGTG Cuts at: 1166 2219 2822 1166 Size: 1053 603 5080 Fragments arranged by size: 5080 1053 603 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3185 1184 Size: 2001 4735 AlwNI CAG_nnn'CTG Cuts at: 3780 4479 6051 3780 Size: 699 1572 4465 Fragments arranged by size: 4465 1572 699 ApaI G_GGCC'C Cuts at: 2625 3181 2625 Size: 556 6180 ApaLI G'TGCA_C Cuts at: 2357 2699 4793 5949 2357 Size: 342 2094 1156 3144 Fragments arranged by size: 3144 2094 1156 342 ApoI r'AATT_y Cuts at: 1493 3389 4045 4271 4423 6532 1493 Size: 1896 656 226 152 2109 1697 Fragments arranged by size: 2109 1896 1697 656 226 152 AseI AT'TA_AT Cuts at: 160 1032 5290 160 Size: 872 4258 1606 Fragments arranged by size: 4258 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 AvaI C'yCGr_G Cuts at: 1870 1943 2947 4061 1870 Size: 73 1004 1114 4545 Fragments arranged by size: 4545 1114 1004 73 BamHI G'GATC_C Cuts at: 4066 4433 4066 Size: 367 6369 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 3497 3977 4057 5512 Size: 325 217 627 273 1437 480 80 1455 Cuts at: 5512 618 Size: 1842 Fragments arranged by size: 1842 1455 1437 627 480 325 273 217 80 BanII G_rGCy'C Cuts at: 1391 2625 2687 3181 3342 4055 1391 Size: 1234 62 494 161 713 4072 Fragments arranged by size: 4072 1234 713 494 161 62 BbeI G_GCGC'C Cuts at: 3501 3501 Size: 6736 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3852 928 Size: 971 1953 3812 Fragments arranged by size: 3812 1953 971 BbvCI CC'TCA_GC Cuts at: 2466 2466 Size: 6736 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4927 4961 1903 Size: 34 2990 34 3678 Fragments arranged by size: 3678 2990 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4628 5838 4628 Size: 1210 5526 BclI T'GATC_A Cuts at: 1548 2325 1548 Size: 777 5959 BfrBI ATG'CAT Cuts at: 3425 3425 Size: 6736 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2440 2841 3116 Size: 107 122 71 631 605 768 401 275 Cuts at: 3116 5347 136 Size: 2231 1525 Fragments arranged by size: 2231 1525 768 631 605 401 275 122 107 71 BglII A'GATC_T Cuts at: 6731 6731 Size: 6736 BlpI GC'TnA_GC Cuts at: 4153 4153 Size: 6736 Bme1580I G_kGCm'C Cuts at: 1790 2065 2361 2625 2703 3181 4797 5953 1790 Size: 275 296 264 78 478 1616 1156 2573 Fragments arranged by size: 2573 1616 1156 478 296 275 264 78 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6736 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2740 3497 4467 Size: 416 53 83 399 24 1323 757 970 Cuts at: 4467 5425 442 Size: 958 1753 Fragments arranged by size: 1753 1323 970 958 757 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6736 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3198 4021 3198 Size: 823 5913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2476 3110 3383 5396 1239 Size: 944 293 634 273 2013 2579 Fragments arranged by size: 2579 2013 944 634 293 273 Bpu10I CC'TnA_GC Cuts at: 2066 2466 3965 2066 Size: 400 1499 4837 Fragments arranged by size: 4837 1499 400 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3257 3326 4861 5726 6024 3257 Size: 69 1535 865 298 3969 Fragments arranged by size: 3969 1535 865 298 69 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2022 2372 2391 3701 5399 882 Size: 617 523 350 19 1310 1698 2219 Fragments arranged by size: 2219 1698 1310 617 523 350 19 BsaAI yAC'GTr Cuts at: 493 1204 2755 4527 493 Size: 711 1551 1772 2702 Fragments arranged by size: 2702 1772 1551 711 BsaBI GATnn'nnATC Cuts at: 6730 6730 Size: 6736 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3498 4543 4925 5560 Size: 53 83 186 936 1965 1045 382 635 Cuts at: 5560 275 Size: 1451 Fragments arranged by size: 1965 1451 1045 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2731 3394 3951 5169 5567 5841 5988 6399 2731 Size: 663 557 1218 398 274 147 411 3068 Fragments arranged by size: 3068 1218 663 557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 3668 3698 1178 Size: 30 165 30 1149 30 1086 30 4216 Fragments arranged by size: 4216 1149 1086 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2935 3488 1186 Size: 1749 553 4434 Fragments arranged by size: 4434 1749 553 BseYI C'CCAG_C Cuts at: 2113 2309 3773 4031 5939 6435 2113 Size: 196 1464 258 1908 496 2414 Fragments arranged by size: 2414 1908 1464 496 258 196 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2760 2996 3191 4040 1163 Size: 573 483 541 236 195 849 3859 Fragments arranged by size: 3859 849 573 541 483 236 195 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2912 4186 4947 5975 1540 Size: 275 186 911 1274 761 1028 2301 Fragments arranged by size: 2301 1274 1028 911 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2361 2703 3342 3732 4797 4882 5953 2361 Size: 342 639 390 1065 85 1071 3144 Fragments arranged by size: 3144 1071 1065 639 390 342 85 BsmI GAATG_Cn' Cuts at: 3916 4247 4340 3916 Size: 331 93 6312 Fragments arranged by size: 6312 331 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2065 2361 2625 2687 2703 3181 3342 Size: 399 275 296 264 62 16 478 161 Cuts at: 3342 3732 4055 4797 4882 5953 1391 Size: 390 323 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 399 390 323 296 275 264 161 85 62 16 BspEI T'CCGG_A Cuts at: 2731 3394 6399 2731 Size: 663 3005 3068 Fragments arranged by size: 3068 3005 663 BspHI T'CATG_A Cuts at: 4625 6355 4625 Size: 1730 5006 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2750 2775 4091 844 Size: 1906 25 1316 3489 Fragments arranged by size: 3489 1906 1316 25 BsrBI CCG'CTC Cuts at: 1394 1823 2056 2077 4623 1394 Size: 429 233 21 2546 3507 Fragments arranged by size: 3507 2546 429 233 21 BsrDI GCAATG_nn' Cuts at: 65 1248 3031 5231 5405 65 Size: 1183 1783 2200 174 1396 Fragments arranged by size: 2200 1783 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2715 4026 5380 5567 1700 Size: 110 905 1311 1354 187 2869 Fragments arranged by size: 2869 1354 1311 905 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6736 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6736 BssSI C'ACGA_G Cuts at: 3520 4796 5808 3520 Size: 1276 1012 4448 Fragments arranged by size: 4448 1276 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6736 BstBI TT'CG_AA Cuts at: 5540 5540 Size: 6736 BstEII G'GTnAC_C Cuts at: 1184 2762 3242 1184 Size: 1578 480 4678 Fragments arranged by size: 4678 1578 480 BstXI CCAn_nnnn'nTGG Cuts at: 2393 3718 2393 Size: 1325 5411 BstYI r'GATC_y Cuts at: 1972 4066 4433 4818 4835 6287 6373 6385 6731 Size: 2094 367 385 17 1452 86 12 346 Cuts at: 6731 1972 Size: 1977 Fragments arranged by size: 2094 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3260 3260 Size: 6736 BtgI C'CryG_G Cuts at: 513 1065 2004 3615 513 Size: 552 939 1611 3634 Fragments arranged by size: 3634 1611 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2407 487 Size: 683 56 1181 4816 Fragments arranged by size: 4816 1181 683 56 BtsI GCAGTG_nn' Cuts at: 3140 3601 3976 4028 4252 5046 5066 3140 Size: 461 375 52 224 794 20 4810 Fragments arranged by size: 4810 794 461 375 224 52 20 ClaI AT'CG_AT Cuts at: 1554 3804 1554 Size: 2250 4486 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6701 DraI TTT'AAA Cuts at: 4040 4040 Size: 6736 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5743 809 Size: 507 4427 1802 Fragments arranged by size: 4427 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2548 2909 3598 Size: 54 1425 50 275 18 718 361 689 Cuts at: 3598 3789 4183 5072 6701 8 Size: 191 394 889 1629 43 Fragments arranged by size: 1629 1425 889 718 689 394 361 275 191 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2909 4183 1537 Size: 275 1097 1274 4090 Fragments arranged by size: 4090 1274 1097 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3458 4666 1353 Size: 2105 1208 3423 Fragments arranged by size: 3423 2105 1208 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2471 3497 5323 5697 5843 387 Size: 1064 138 882 1026 1826 374 146 1280 Fragments arranged by size: 1826 1280 1064 1026 882 374 146 138 EcoICRI GAG'CTC Cuts at: 3340 3340 Size: 6736 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2476 2673 2815 3110 3323 3375 Size: 393 551 293 197 142 295 213 52 Cuts at: 3375 3383 4799 5396 6183 6451 1239 Size: 8 1416 597 787 268 1524 Fragments arranged by size: 1524 1416 787 597 551 393 295 293 268 213 197 142 52 8 EcoNI CCTnn'n_nnAGG Cuts at: 2629 2629 Size: 6736 EcoO109I rG'GnC_Cy Cuts at: 2227 2622 3177 3178 3485 3984 2227 Size: 395 555 1 307 499 4979 Fragments arranged by size: 4979 555 499 395 307 1 EcoRI G'AATT_C Cuts at: 4045 4423 4045 Size: 378 6358 EcoRV GAT'ATC Cuts at: 3622 3622 Size: 6736 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3328 3328 Size: 6736 FspI TGC'GCA Cuts at: 5242 5242 Size: 6736 HaeII r_GCGC'y Cuts at: 1678 2083 2435 3417 3501 5883 1678 Size: 405 352 982 84 2382 2531 Fragments arranged by size: 2531 2382 982 405 352 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 3524 3556 3668 Size: 32 135 32 447 32 1473 32 112 Cuts at: 3668 3700 5377 5409 5451 5483 1373 Size: 32 1677 32 42 32 2626 Fragments arranged by size: 2626 1677 1473 447 135 112 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2017 2155 2581 2860 3754 4080 Size: 988 281 79 138 426 279 894 326 Cuts at: 4080 4326 669 Size: 246 3079 Fragments arranged by size: 3079 988 894 426 326 281 279 246 138 79 HindIII A'AGCT_T Cuts at: 748 2777 748 Size: 2029 4707 HpaI GTT'AAC Cuts at: 4326 4326 Size: 6736 KasI G'GCGC_C Cuts at: 3497 3497 Size: 6736 KpnI G_GTAC'C Cuts at: 4061 4061 Size: 6736 MfeI C'AATT_G Cuts at: 4335 4335 Size: 6736 MluI A'CGCG_T Cuts at: 6612 6612 Size: 6736 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3015 3903 5850 6034 1305 Size: 1710 888 1947 184 2007 Fragments arranged by size: 2007 1947 1710 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2550 3791 10 Size: 54 1425 343 718 1241 2955 Fragments arranged by size: 2955 1425 1241 718 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2219 2387 2648 2822 3024 3252 4694 Size: 648 1053 168 261 174 202 228 1442 Cuts at: 4694 5053 5212 518 Size: 359 159 2042 Fragments arranged by size: 2042 1442 1053 648 359 261 228 202 174 168 159 MspA1I CmG'CkG Cuts at: 1606 2233 2554 2619 2693 2908 3327 3446 3901 Size: 627 321 65 74 215 419 119 455 Cuts at: 3901 4152 4829 5977 6222 1606 Size: 251 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 627 455 419 321 251 245 215 119 74 65 NaeI GCC'GGC Cuts at: 4028 4028 Size: 6736 NarI GG'CG_CC Cuts at: 3498 3498 Size: 6736 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5245 Fragments arranged by size: 5245 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6736 NgoMIV G'CCGG_C Cuts at: 4026 4026 Size: 6736 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6736 NotI GC'GGCC_GC Cuts at: 4183 4183 Size: 6736 NsiI A_TGCA'T Cuts at: 3427 3427 Size: 6736 NspI r_CATG'y Cuts at: 1132 1229 2357 2392 3059 4094 5639 1132 Size: 97 1128 35 667 1035 1545 2229 Fragments arranged by size: 2229 1545 1128 1035 667 97 35 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6736 PciI A'CATG_T Cuts at: 2388 3055 5635 2388 Size: 667 2580 3489 Fragments arranged by size: 3489 2580 667 PflMI CCAn_nnn'nTGG Cuts at: 1108 2557 1108 Size: 1449 5287 PmeI GTTT'AAAC Cuts at: 4040 4040 Size: 6736 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4805 4837 6318 6350 4805 Size: 32 1481 32 5191 Fragments arranged by size: 5191 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2227 3485 2227 Size: 1258 5478 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6736 PsiI TTA'TAA Cuts at: 4306 4306 Size: 6736 PspOMI G'GGCC_C Cuts at: 2621 3177 2621 Size: 556 6180 PstI C_TGCA'G Cuts at: 830 2136 3765 4088 830 Size: 1306 1629 323 3478 Fragments arranged by size: 3478 1629 1306 323 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6736 PvuII CAG'CTG Cuts at: 2554 3446 2554 Size: 892 5844 SacI G_AGCT'C Cuts at: 3342 3342 Size: 6736 SalI G'TCGA_C Cuts at: 1936 4078 1936 Size: 2142 4594 SanDI GG'GwC_CC Cuts at: 2227 2227 Size: 6736 SbfI CC_TGCA'GG Cuts at: 4088 4088 Size: 6736 ScaI AGT'ACT Cuts at: 2543 4984 6696 2543 Size: 2441 1712 2583 Fragments arranged by size: 2583 2441 1712 SexAI A'CCwGG_T Cuts at: 3487 3717 3487 Size: 230 6506 SfcI C'TryA_G Cuts at: 826 1045 2132 3761 4084 5219 5900 6091 826 Size: 219 1087 1629 323 1135 681 191 1471 Fragments arranged by size: 1629 1471 1135 1087 681 323 219 191 SfoI GGC'GCC Cuts at: 3499 3499 Size: 6736 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6736 SmaI CCC'GGG Cuts at: 1872 4063 1872 Size: 2191 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3272 3341 4840 5741 6003 820 Size: 197 926 1329 69 1499 901 262 1553 Fragments arranged by size: 1553 1499 1329 926 901 262 197 69 SnaBI TAC'GTA Cuts at: 493 2755 4527 493 Size: 2262 1772 2702 Fragments arranged by size: 2702 2262 1772 SpeI A'CTAG_T Cuts at: 152 2854 152 Size: 2702 4034 SphI G_CATG'C Cuts at: 1132 4094 1132 Size: 2962 3774 SspI AAT'ATT Cuts at: 5 52 4660 5 Size: 47 4608 2081 Fragments arranged by size: 4608 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6736 StyI C'CwwG_G Cuts at: 513 1065 2004 3733 4175 513 Size: 552 939 1729 442 3074 Fragments arranged by size: 3074 1729 939 552 442 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4763 4780 4933 5118 5563 325 Size: 1255 3183 17 153 185 445 1498 Fragments arranged by size: 3183 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2541 4982 6694 Size: 274 80 33 51 1443 564 2441 1712 Cuts at: 6694 96 Size: 138 Fragments arranged by size: 2441 1712 1443 564 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2552 2584 6696 6728 2552 Size: 32 4112 32 2560 Fragments arranged by size: 4112 2560 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6736 XbaI T'CTAG_A Cuts at: 4072 4072 Size: 6736 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6736 XmaI C'CCGG_G Cuts at: 1870 4061 1870 Size: 2191 4545 XmnI GAAnn'nnTTC Cuts at: 4865 4865 Size: 6736 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4544 276 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I DraIII FseI FspAI NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI XcmI