Restriction map for
pFN21AB6912
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
DraI DraII DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI SacI
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 169 GACGTC GACGT^C
AflIII 396 ACACGT A^C[GA][CT]GT
BanII 562 GAGCCC G[GA]GC[CT]^C
BciVI 584 GGATAC GTATCC(6/5)
BsaI 115 GAGACC GGTCTC(1/5)
BsgI 577 GTGCAG GTGCAG(16/14)
BsrBI 214 CCGCTC CCGCTC(-3/-3)
BssHII 54 GCGCGC G^CGCGC
BssSI 433 CACGAG CACGAG(-5/-1)
BstF5I 348 CATCC GGATG(2/0)
CpoI 152 CGGTCCG CG^G[AT]CCG
DdeI 371 CTGAG C^T[ACGT]AG
EarI 217 CTCTTC CTCTTC(1/4)
EcoT14I 638 CCAAGG C^C[AT][AT]GG
FokI 348 CATCC GGATG(9/13)
HinfI 88 GACTC G^A[ACGT]TC
HphI 449 TCACC GGTGA(8/7)
Ksp632I 217 CTCTTC CTCTTC(1/4)
KspI 22 CCGCGG CCGC^GG
MaeIII 238 GTCAC ^GT[ACGT]AC
MslI 291 CACGCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 88 GACTC GAGTC(4/5)
PvuII 196 CAGCTG CAG^CTG
RsrII 152 CGGTCCG CG^G[AT]CCG
SacII 22 CCGCGG CCGC^GG
SapI 216 GCTCTTC GCTCTTC(1/4)
SfaNI 347 GCATC GCATC(5/9)
SgrAI 461 CGCCGGCG C[GA]^CCGG[CT]G
StyI 638 CCAAGG C^C[AT][AT]GG
TaqI 392 TCGA T^CGA
Tsp45I 238 GTCAC ^GT[GC]AC
TthHB8I 392 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 405 GTAC GT^AC
495 GTAC
AvaII 153 GGTCC G^G[AT]CC
327 GGACC
BbeI 284 GGCGCC GGCGC^C
465 GGCGCC
BcnI 156 CCGGG CC^[GC]GG
510 CCGGG
BsiEI 127 CGGCCG CG[GA][CT]^CG
204 CGGCCG
BsmAI 115 GAGAC GTCTC(1/5)
545 GAGAC
BsrI 281 ACTGG ACTGG(1/-1)
413 ACTGG
DpnII 30 GATC ^GATC
390 GATC
DsaI 22 CCGCGG C^C[GA][CT]GG
330 CCACGG
EaeI 127 CGGCCG [CT]^GGCC[GA]
204 CGGCCG
EagI 127 CGGCCG C^GGCCG
204 CGGCCG
EclXI 127 CGGCCG C^GGCCG
204 CGGCCG
Eco52I 127 CGGCCG C^GGCCG
204 CGGCCG
HaeII 284 GGCGCC [GA]GCGC^[CT]
465 GGCGCC
KasI 284 GGCGCC G^GCGCC
465 GGCGCC
MaeII 170 ACGT A^CGT
398 ACGT
MboI 30 GATC ^GATC
390 GATC
MspA1I 22 CCGCGG C[AC]G^C[GT]G
196 CAGCTG
NaeI 143 GCCGGC GCC^GGC
462 GCCGGC
NarI 284 GGCGCC GG^CGCC
465 GGCGCC
NciI 156 CCGGG CC^[GC]GG
510 CCGGG
NdeII 30 GATC ^GATC
390 GATC
NgoMIV 143 GCCGGC G^CCGGC
462 GCCGGC
RsaI 405 GTAC GT^AC
495 GTAC
Sau3AI 30 GATC ^GATC
390 GATC
SfoI 284 GGCGCC GGC^GCC
465 GGCGCC
StuI 338 AGGCCT AGG^CCT
443 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbsI BbvI BmyI BpuAI BsaHI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MboII
MnlI MspI MvaI MvnI MwoI
NlaIII NlaIV Sau96I ScrFI TseI