(Circular) (Six-base) MAPSORT of: pFN21AB6912.seq Check: 4590 from: 1 to: 5359 pFN21AB6912 5359 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2178 3169 278 Size: 53 83 186 936 642 991 2468 Fragments arranged by size: 2468 991 936 642 186 83 53 AccI GT'mk_AC Cuts at: 1937 2702 1937 Size: 765 4594 Acc65I G'GTAC_C Cuts at: 2680 2680 Size: 5359 AclI AA'CG_TT Cuts at: 1563 3486 3859 1563 Size: 1923 373 3063 Fragments arranged by size: 3063 1923 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3422 4806 5074 1632 Size: 1790 1384 268 1917 Fragments arranged by size: 1917 1790 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5162 AflIII A'CryG_T Cuts at: 1203 2401 4258 5235 1203 Size: 1198 1857 977 1327 Fragments arranged by size: 1857 1327 1198 977 AgeI A'CCGG_T Cuts at: 4190 4190 Size: 5359 AhdI GACnn_n'nnGTC Cuts at: 4088 4088 Size: 5359 AleI CACnn'nnGTG Cuts at: 1166 2300 1166 Size: 1134 4225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5359 AlwNI CAG_nnn'CTG Cuts at: 3102 4674 3102 Size: 1572 3787 ApaLI G'TGCA_C Cuts at: 3416 4572 3416 Size: 1156 4203 ApoI r'AATT_y Cuts at: 1493 2668 2894 3046 5155 1493 Size: 1175 226 152 2109 1697 Fragments arranged by size: 2109 1697 1175 226 152 AseI AT'TA_AT Cuts at: 160 1032 3913 160 Size: 872 2881 1606 Fragments arranged by size: 2881 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 AvaI C'yCGr_G Cuts at: 1870 1943 2684 1870 Size: 73 741 4545 Fragments arranged by size: 4545 741 73 BamHI G'GATC_C Cuts at: 2689 3056 2689 Size: 367 4992 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2289 2428 2470 2648 2680 Size: 325 217 627 502 139 42 178 32 Cuts at: 2680 4135 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 502 325 217 178 139 42 32 BanII G_rGCy'C Cuts at: 1391 2571 2678 1391 Size: 1180 107 4072 Fragments arranged by size: 4072 1180 107 BbeI G_GCGC'C Cuts at: 2293 2474 2293 Size: 181 5178 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2378 2484 2634 928 Size: 971 479 106 150 3653 Fragments arranged by size: 3653 971 479 150 106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2638 2672 3550 3584 1903 Size: 34 701 34 878 34 3678 Fragments arranged by size: 3678 878 701 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2583 3251 4461 2583 Size: 668 1210 3481 Fragments arranged by size: 3481 1210 668 BclI T'GATC_A Cuts at: 1548 1548 Size: 5359 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3970 136 Size: 107 122 71 631 605 2298 1525 Fragments arranged by size: 2298 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5354 5354 Size: 5359 BlpI GC'TnA_GC Cuts at: 2776 2776 Size: 5359 Bme1580I G_kGCm'C Cuts at: 1790 2433 2651 3420 4576 1790 Size: 643 218 769 1156 2573 Fragments arranged by size: 2573 1156 769 643 218 BmgBI CAC'GTC Cuts at: 1318 2404 1318 Size: 1086 4273 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3090 4048 442 Size: 416 53 83 399 24 1673 958 1753 Fragments arranged by size: 1753 1673 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4019 1239 Size: 2780 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3484 4349 4647 3484 Size: 865 298 4196 Fragments arranged by size: 4196 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2114 4022 882 Size: 617 615 1908 2219 Fragments arranged by size: 2219 1908 617 615 BsaAI yAC'GTr Cuts at: 493 1204 3150 493 Size: 711 1946 2702 Fragments arranged by size: 2702 1946 711 BsaBI GATnn'nnATC Cuts at: 5353 5353 Size: 5359 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2146 2175 2290 2471 Size: 53 83 186 936 613 29 115 181 Cuts at: 2471 3166 3548 4183 275 Size: 695 382 635 1451 Fragments arranged by size: 1451 936 695 635 613 382 186 181 115 83 53 29 BsaWI w'CCGG_w Cuts at: 3792 4190 4464 4611 5022 3792 Size: 398 274 147 411 4129 Fragments arranged by size: 4129 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5134 Fragments arranged by size: 5134 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 2459 2501 2579 1186 Size: 1270 3 42 78 3966 Fragments arranged by size: 3966 1270 78 42 3 BseYI C'CCAG_C Cuts at: 2097 4562 5058 2097 Size: 2465 496 2398 Fragments arranged by size: 2465 2398 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2603 1163 Size: 573 867 3919 Fragments arranged by size: 3919 867 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2135 2212 2809 3570 4598 1540 Size: 275 186 134 77 597 761 1028 2301 Fragments arranged by size: 2301 1028 761 597 275 186 134 77 BsiHKAI G_wGCw'C Cuts at: 3420 3505 4576 3420 Size: 85 1071 4203 Fragments arranged by size: 4203 1071 85 BsmI GAATG_Cn' Cuts at: 2870 2963 2870 Size: 93 5266 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2433 2571 2651 2678 3420 3505 4576 Size: 399 643 138 80 27 742 85 1071 Cuts at: 4576 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 643 399 138 85 80 27 BspEI T'CCGG_A Cuts at: 5022 5022 Size: 5359 BspHI T'CATG_A Cuts at: 3248 4978 3248 Size: 1730 3629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2714 844 Size: 1870 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2221 3246 1394 Size: 429 398 1025 3507 Fragments arranged by size: 3507 1025 429 398 BsrDI GCAATG_nn' Cuts at: 65 1248 3854 4028 65 Size: 1183 2606 174 1396 Fragments arranged by size: 2606 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 2155 2467 4003 4190 1700 Size: 110 338 7 312 1536 187 2869 Fragments arranged by size: 2869 1536 338 312 187 110 7 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5359 BssHII G'CGCG_C Cuts at: 1925 2059 1925 Size: 134 5225 BssSI C'ACGA_G Cuts at: 2438 3419 4431 2438 Size: 981 1012 3366 Fragments arranged by size: 3366 1012 981 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5359 BstBI TT'CG_AA Cuts at: 4163 4163 Size: 5359 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5359 BstYI r'GATC_y Cuts at: 1972 2689 3056 3441 3458 4910 4996 5008 5354 Size: 717 367 385 17 1452 86 12 346 Cuts at: 5354 1972 Size: 1977 Fragments arranged by size: 1977 1452 717 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2027 2335 513 Size: 552 939 23 308 3537 Fragments arranged by size: 3537 939 552 308 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4620 Fragments arranged by size: 4620 683 56 BtsI GCAGTG_nn' Cuts at: 2875 3669 3689 2875 Size: 794 20 4545 Fragments arranged by size: 4545 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5359 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5324 DraI TTT'AAA Cuts at: 2663 2663 Size: 5359 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4366 809 Size: 507 3050 1802 Fragments arranged by size: 3050 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2132 2209 2806 Size: 54 1425 50 275 18 302 77 597 Cuts at: 2806 3695 5324 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 597 302 275 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2132 2209 2806 1537 Size: 275 320 77 597 4090 Fragments arranged by size: 4090 597 320 275 77 EarI CTCTTCn'nnn_ Cuts at: 1353 2228 3289 1353 Size: 875 1061 3423 Fragments arranged by size: 3423 1061 875 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2168 3946 4320 4466 387 Size: 1064 138 579 1778 374 146 1280 Fragments arranged by size: 1778 1280 1064 579 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3422 4019 4806 5074 1239 Size: 393 1790 597 787 268 1524 Fragments arranged by size: 1790 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2668 3046 2668 Size: 378 4981 FspI TGC'GCA Cuts at: 3865 3865 Size: 5359 HaeII r_GCGC'y Cuts at: 1678 2293 2474 4506 1678 Size: 615 181 2032 2531 Fragments arranged by size: 2531 2032 615 181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4000 4032 4074 4106 1373 Size: 32 135 32 2428 32 42 32 2626 Fragments arranged by size: 2626 2428 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2703 2949 669 Size: 988 281 765 246 3079 Fragments arranged by size: 3079 988 765 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5359 HpaI GTT'AAC Cuts at: 2949 2949 Size: 5359 KasI G'GCGC_C Cuts at: 2289 2470 2289 Size: 181 5178 KpnI G_GTAC'C Cuts at: 2684 2684 Size: 5359 MfeI C'AATT_G Cuts at: 2958 2958 Size: 5359 MluI A'CGCG_T Cuts at: 5235 5235 Size: 5359 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4473 4657 1305 Size: 3168 184 2007 Fragments arranged by size: 3168 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3537 Fragments arranged by size: 3537 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 3317 3676 3835 518 Size: 648 1134 1017 359 159 2042 Fragments arranged by size: 2042 1134 1017 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2203 2775 3452 4600 4845 1606 Size: 423 174 572 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 572 423 245 174 NaeI GCC'GGC Cuts at: 2150 2469 2150 Size: 319 5040 NarI GG'CG_CC Cuts at: 2290 2471 2290 Size: 181 5178 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3868 Fragments arranged by size: 3868 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5359 NgoMIV G'CCGG_C Cuts at: 2148 2467 2148 Size: 319 5040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5359 NotI GC'GGCC_GC Cuts at: 2806 2806 Size: 5359 NspI r_CATG'y Cuts at: 1132 1229 2717 4262 1132 Size: 97 1488 1545 2229 Fragments arranged by size: 2229 1545 1488 97 PasI CC'CwG_GG Cuts at: 1348 2604 1348 Size: 1256 4103 PciI A'CATG_T Cuts at: 4258 4258 Size: 5359 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5359 PmeI GTTT'AAAC Cuts at: 2663 2663 Size: 5359 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3428 3460 4941 4973 3428 Size: 32 1481 32 3814 Fragments arranged by size: 3814 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5359 PsiI TTA'TAA Cuts at: 2929 2929 Size: 5359 PstI C_TGCA'G Cuts at: 830 2711 830 Size: 1881 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5359 PvuII CAG'CTG Cuts at: 2203 2203 Size: 5359 RsrII CG'GwC_CG Cuts at: 2158 2158 Size: 5359 SacII CC_GC'GG Cuts at: 2030 2030 Size: 5359 SalI G'TCGA_C Cuts at: 1936 2701 1936 Size: 765 4594 SapI GCTCTTCn'nnn_ Cuts at: 2228 2228 Size: 5359 SbfI CC_TGCA'GG Cuts at: 2711 2711 Size: 5359 ScaI AGT'ACT Cuts at: 3607 5319 3607 Size: 1712 3647 SfcI C'TryA_G Cuts at: 826 1045 2707 3842 4523 4714 826 Size: 219 1662 1135 681 191 1471 Fragments arranged by size: 1662 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2291 2472 2291 Size: 181 5178 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 SgrAI Cr'CCGG_yG Cuts at: 1700 2467 1700 Size: 767 4592 SmaI CCC'GGG Cuts at: 1872 2686 1872 Size: 814 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3463 4364 4626 820 Size: 197 926 1520 901 262 1553 Fragments arranged by size: 1553 1520 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3150 493 Size: 2657 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5359 SphI G_CATG'C Cuts at: 1132 2717 1132 Size: 1585 3774 SspI AAT'ATT Cuts at: 5 52 3283 5 Size: 47 3231 2081 Fragments arranged by size: 3231 2081 47 StuI AGG'CCT Cuts at: 1517 2345 2450 1517 Size: 828 105 4426 Fragments arranged by size: 4426 828 105 StyI C'CwwG_G Cuts at: 513 1065 2004 2643 2798 513 Size: 552 939 639 155 3074 Fragments arranged by size: 3074 939 639 552 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3386 3403 3556 3741 4186 325 Size: 1255 1806 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3605 5317 96 Size: 274 80 33 51 1443 1628 1712 138 Fragments arranged by size: 1712 1628 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5319 5351 5319 Size: 32 5327 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5359 XbaI T'CTAG_A Cuts at: 2695 2695 Size: 5359 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5359 XmaI C'CCGG_G Cuts at: 1870 2684 1870 Size: 814 4545 XmnI GAAnn'nnTTC Cuts at: 3488 3488 Size: 5359 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2176 3167 276 Size: 53 83 186 936 642 991 2468 Fragments arranged by size: 2468 991 936 642 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SacI SanDI SexAI SfiI SrfI SwaI XcmI