(Circular) (Six-base) MAPSORT of: pFN21AB6203.seq Check: 7381 from: 1 to: 6694 pFN21AB6203 6694 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3358 3358 Size: 6694 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4504 278 Size: 53 83 186 936 2968 2468 Fragments arranged by size: 2968 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4037 1937 Size: 2100 4594 Acc65I G'GTAC_C Cuts at: 4015 4015 Size: 6694 AclI AA'CG_TT Cuts at: 1563 4821 5194 1563 Size: 3258 373 3063 Fragments arranged by size: 3258 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2107 2984 3152 3683 4757 6141 6409 1632 Size: 475 877 168 531 1074 1384 268 1917 Fragments arranged by size: 1917 1384 1074 877 531 475 268 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6497 AflIII A'CryG_T Cuts at: 1203 2541 2823 2931 3151 5593 6570 1203 Size: 1338 282 108 220 2442 977 1327 Fragments arranged by size: 2442 1338 1327 977 282 220 108 AgeI A'CCGG_T Cuts at: 5525 5525 Size: 6694 AhdI GACnn_n'nnGTC Cuts at: 2116 3403 5423 2116 Size: 1287 2020 3387 Fragments arranged by size: 3387 2020 1287 AleI CACnn'nnGTG Cuts at: 1166 2339 3947 1166 Size: 1173 1608 3913 Fragments arranged by size: 3913 1608 1173 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3965 1184 Size: 2781 3913 AlwNI CAG_nnn'CTG Cuts at: 3343 3492 4437 6009 3343 Size: 149 945 1572 4028 Fragments arranged by size: 4028 1572 945 149 ApaI G_GGCC'C Cuts at: 2432 3192 3816 2432 Size: 760 624 5310 Fragments arranged by size: 5310 760 624 ApaLI G'TGCA_C Cuts at: 3883 3932 4751 5907 3883 Size: 49 819 1156 4670 Fragments arranged by size: 4670 1156 819 49 ApoI r'AATT_y Cuts at: 1493 4003 4229 4381 6490 1493 Size: 2510 226 152 2109 1697 Fragments arranged by size: 2510 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5248 160 Size: 872 4216 1606 Fragments arranged by size: 4216 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 AvaI C'yCGr_G Cuts at: 1870 1943 2788 2843 3183 4019 1870 Size: 73 845 55 340 836 4545 Fragments arranged by size: 4545 845 836 340 73 55 BamHI G'GATC_C Cuts at: 4024 4391 4024 Size: 367 6327 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2444 2884 3941 3988 Size: 325 217 627 282 375 440 1057 47 Cuts at: 3988 4015 5470 618 Size: 27 1455 1842 Fragments arranged by size: 1842 1455 1057 627 440 375 325 282 217 47 27 BanII G_rGCy'C Cuts at: 1391 2432 2796 2841 3192 3245 3816 4013 1391 Size: 1041 364 45 351 53 571 197 4072 Fragments arranged by size: 4072 1041 571 364 351 197 53 45 BbeI G_GCGC'C Cuts at: 2888 2888 Size: 6694 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2535 2573 2866 3097 928 Size: 971 636 38 293 231 4525 Fragments arranged by size: 4525 971 636 293 231 38 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2471 2505 4885 4919 1903 Size: 34 534 34 2380 34 3678 Fragments arranged by size: 3678 2380 534 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4586 5796 4586 Size: 1210 5484 BclI T'GATC_A Cuts at: 1548 1548 Size: 6694 BfrBI ATG'CAT Cuts at: 3639 3639 Size: 6694 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3811 5305 136 Size: 107 122 71 631 605 2139 1494 1525 Fragments arranged by size: 2139 1525 1494 631 605 122 107 71 BglII A'GATC_T Cuts at: 6689 6689 Size: 6694 BlpI GC'TnA_GC Cuts at: 4111 4111 Size: 6694 Bme1580I G_kGCm'C Cuts at: 1790 2072 2432 3192 3364 3816 3887 3936 3993 Size: 282 360 760 172 452 71 49 57 Cuts at: 3993 4755 5911 1790 Size: 762 1156 2573 Fragments arranged by size: 2573 1156 762 760 452 360 282 172 71 57 49 BmgBI CAC'GTC Cuts at: 1318 2118 2826 1318 Size: 800 708 5186 Fragments arranged by size: 5186 800 708 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3242 3881 4425 Size: 416 53 83 399 24 1825 639 544 Cuts at: 4425 5383 442 Size: 958 1753 Fragments arranged by size: 1825 1753 958 639 544 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6694 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2495 2495 Size: 6694 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 2855 3860 3863 3884 5354 1239 Size: 918 698 1005 3 21 1470 2579 Fragments arranged by size: 2579 1470 1005 918 698 21 3 Bpu10I CC'TnA_GC Cuts at: 2558 3431 2558 Size: 873 5821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4819 5684 5982 4819 Size: 865 298 5531 Fragments arranged by size: 5531 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5357 882 Size: 617 3858 2219 Fragments arranged by size: 3858 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2611 2755 3418 4485 493 Size: 711 1407 144 663 1067 2702 Fragments arranged by size: 2702 1407 1067 711 663 144 BsaBI GATnn'nnATC Cuts at: 6688 6688 Size: 6694 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2885 4501 4883 5518 Size: 53 83 186 936 1352 1616 382 635 Cuts at: 5518 275 Size: 1451 Fragments arranged by size: 1616 1451 1352 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5127 5525 5799 5946 6357 5127 Size: 398 274 147 411 5464 Fragments arranged by size: 5464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2589 2619 3836 3861 3866 Size: 30 165 30 1186 30 1217 25 5 Cuts at: 3866 3891 1178 Size: 25 3981 Fragments arranged by size: 3981 1217 1186 165 30 30 30 25 25 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3035 3038 3857 1186 Size: 1849 3 819 4023 Fragments arranged by size: 4023 1849 819 3 BseYI C'CCAG_C Cuts at: 2065 2910 3277 3311 3363 3673 5897 6393 2065 Size: 845 367 34 52 310 2224 496 2366 Fragments arranged by size: 2366 2224 845 496 367 310 52 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2098 2698 2956 3392 1163 Size: 573 362 600 258 436 4465 Fragments arranged by size: 4465 600 573 436 362 258 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4144 4905 5933 1540 Size: 275 186 1828 315 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 315 275 186 BsiHKAI G_wGCw'C Cuts at: 2564 2607 2841 3887 3936 4755 4840 5911 2564 Size: 43 234 1046 49 819 85 1071 3347 Fragments arranged by size: 3347 1071 1046 819 234 85 49 43 BsmI GAATG_Cn' Cuts at: 4205 4298 4205 Size: 93 6601 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2432 2564 2607 2796 2841 3192 Size: 399 282 360 132 43 189 45 351 Cuts at: 3192 3245 3364 3816 3887 3936 3993 4013 4755 Size: 53 119 452 71 49 57 20 742 Cuts at: 4755 4840 5911 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 452 399 360 351 282 189 132 119 85 71 57 53 49 45 43 20 BspEI T'CCGG_A Cuts at: 6357 6357 Size: 6694 BspHI T'CATG_A Cuts at: 2895 4583 6313 2895 Size: 1688 1730 3276 Fragments arranged by size: 3276 1730 1688 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2541 3358 4049 844 Size: 1697 817 691 3489 Fragments arranged by size: 3489 1697 817 691 BsrBI CCG'CTC Cuts at: 1394 1823 4581 1394 Size: 429 2758 3507 Fragments arranged by size: 3507 2758 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3322 5189 5363 65 Size: 1183 2074 1867 174 1396 Fragments arranged by size: 2074 1867 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2375 3324 5338 5525 1700 Size: 110 565 949 2014 187 2869 Fragments arranged by size: 2869 2014 949 565 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6694 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6694 BssSI C'ACGA_G Cuts at: 4754 5766 4754 Size: 1012 5682 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6694 BstBI TT'CG_AA Cuts at: 3549 5498 3549 Size: 1949 4745 BstEII G'GTnAC_C Cuts at: 1184 2230 1184 Size: 1046 5648 BstXI CCAn_nnnn'nTGG Cuts at: 2455 2455 Size: 6694 BstYI r'GATC_y Cuts at: 1972 4024 4391 4776 4793 6245 6331 6343 6689 Size: 2052 367 385 17 1452 86 12 346 Cuts at: 6689 1972 Size: 1977 Fragments arranged by size: 2052 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2106 3018 2106 Size: 912 5782 BtgI C'CryG_G Cuts at: 513 1065 2004 2337 2493 3099 3408 3529 513 Size: 552 939 333 156 606 309 121 3678 Fragments arranged by size: 3678 939 606 552 333 309 156 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5955 Fragments arranged by size: 5955 683 56 BtsI GCAGTG_nn' Cuts at: 3934 4210 5004 5024 3934 Size: 276 794 20 5604 Fragments arranged by size: 5604 794 276 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6694 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6659 DraI TTT'AAA Cuts at: 3998 3998 Size: 6694 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2870 5701 809 Size: 507 1554 2831 1802 Fragments arranged by size: 2831 1802 1554 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2613 2982 Size: 54 1425 50 275 18 420 363 369 Cuts at: 2982 3477 3681 3826 4141 5030 6659 8 Size: 495 204 145 315 889 1629 43 Fragments arranged by size: 1629 1425 889 495 420 369 363 315 275 204 145 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 4141 1537 Size: 275 2014 315 4090 Fragments arranged by size: 4090 2014 315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3296 3719 4624 1353 Size: 1943 423 905 3423 Fragments arranged by size: 3423 1943 905 423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3755 5281 5655 5801 387 Size: 1064 138 2166 1526 374 146 1280 Fragments arranged by size: 2166 1526 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2839 2839 Size: 6694 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2107 2157 2855 2984 3152 3683 3860 Size: 393 475 50 698 129 168 531 177 Cuts at: 3860 3863 3884 4757 5354 6141 6409 1239 Size: 3 21 873 597 787 268 1524 Fragments arranged by size: 1524 873 787 698 597 531 475 393 268 177 168 129 50 21 3 EcoO109I rG'GnC_Cy Cuts at: 2415 2428 2429 2693 2761 3188 3189 3340 3376 Size: 13 1 264 68 427 1 151 36 Cuts at: 3376 3489 3538 2415 Size: 113 49 5571 Fragments arranged by size: 5571 427 264 151 113 68 49 36 13 1 1 EcoRI G'AATT_C Cuts at: 4003 4381 4003 Size: 378 6316 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2599 2599 Size: 6694 FspI TGC'GCA Cuts at: 2359 3953 5200 2359 Size: 1594 1247 3853 Fragments arranged by size: 3853 1594 1247 HaeII r_GCGC'y Cuts at: 1678 2888 5841 1678 Size: 1210 2953 2531 Fragments arranged by size: 2953 2531 1210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 3389 3421 5335 Size: 32 135 32 530 32 1255 32 1914 Cuts at: 5335 5367 5409 5441 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1914 1255 530 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4038 4284 669 Size: 988 281 2100 246 3079 Fragments arranged by size: 3079 2100 988 281 246 HindIII A'AGCT_T Cuts at: 748 3744 748 Size: 2996 3698 HpaI GTT'AAC Cuts at: 4284 4284 Size: 6694 KasI G'GCGC_C Cuts at: 2884 2884 Size: 6694 KpnI G_GTAC'C Cuts at: 4019 4019 Size: 6694 MfeI C'AATT_G Cuts at: 4293 4293 Size: 6694 MluI A'CGCG_T Cuts at: 2931 3151 6570 2931 Size: 220 3419 3055 Fragments arranged by size: 3419 3055 220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2853 5808 5992 1305 Size: 1548 2955 184 2007 Fragments arranged by size: 2955 2007 1548 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2615 2984 3479 10 Size: 54 1425 343 783 369 495 3225 Fragments arranged by size: 3225 1425 783 495 369 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2339 3773 3947 4652 5011 5170 518 Size: 648 1173 1434 174 705 359 159 2042 Fragments arranged by size: 2042 1434 1173 705 648 359 174 159 MspA1I CmG'CkG Cuts at: 1606 2339 3277 3303 3831 4110 4787 5935 6180 Size: 733 938 26 528 279 677 1148 245 Cuts at: 6180 1606 Size: 2120 Fragments arranged by size: 2120 1148 938 733 677 528 279 245 26 NaeI GCC'GGC Cuts at: 3326 3326 Size: 6694 NarI GG'CG_CC Cuts at: 2885 2885 Size: 6694 NcoI C'CATG_G Cuts at: 513 1065 2004 2493 513 Size: 552 939 489 4714 Fragments arranged by size: 4714 939 552 489 NdeI CA'TA_TG Cuts at: 387 387 Size: 6694 NgoMIV G'CCGG_C Cuts at: 3324 3324 Size: 6694 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6694 NotI GC'GGCC_GC Cuts at: 4141 4141 Size: 6694 NsiI A_TGCA'T Cuts at: 3641 3641 Size: 6694 NspI r_CATG'y Cuts at: 1132 1229 2545 3772 4052 5597 1132 Size: 97 1316 1227 280 1545 2229 Fragments arranged by size: 2229 1545 1316 1227 280 97 PasI CC'CwG_GG Cuts at: 1348 2011 2141 2219 1348 Size: 663 130 78 5823 Fragments arranged by size: 5823 663 130 78 PciI A'CATG_T Cuts at: 2541 5593 2541 Size: 3052 3642 PflMI CCAn_nnn'nTGG Cuts at: 1108 2599 3170 1108 Size: 1491 571 4632 Fragments arranged by size: 4632 1491 571 PfoI T'CCnGG_A Cuts at: 2772 3507 2772 Size: 735 5959 PmeI GTTT'AAAC Cuts at: 3998 3998 Size: 6694 PmlI CAC'GTG Cuts at: 2611 3418 2611 Size: 807 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4763 4795 6276 6308 4763 Size: 32 1481 32 5149 Fragments arranged by size: 5149 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2415 2693 3489 2415 Size: 278 796 5620 Fragments arranged by size: 5620 796 278 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6694 PsiI TTA'TAA Cuts at: 4264 4264 Size: 6694 PspOMI G'GGCC_C Cuts at: 2428 3188 3812 2428 Size: 760 624 5310 Fragments arranged by size: 5310 760 624 PspXI vC'TCGA_Gb Cuts at: 3183 3183 Size: 6694 PstI C_TGCA'G Cuts at: 830 2096 2538 3348 4046 830 Size: 1266 442 810 698 3478 Fragments arranged by size: 3478 1266 810 698 442 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6694 PvuII CAG'CTG Cuts at: 3303 3303 Size: 6694 SacI G_AGCT'C Cuts at: 2841 2841 Size: 6694 SacII CC_GC'GG Cuts at: 2340 2340 Size: 6694 SalI G'TCGA_C Cuts at: 1936 4036 1936 Size: 2100 4594 SanDI GG'GwC_CC Cuts at: 2693 2693 Size: 6694 SapI GCTCTTCn'nnn_ Cuts at: 3719 3719 Size: 6694 SbfI CC_TGCA'GG Cuts at: 2538 3348 4046 2538 Size: 810 698 5186 Fragments arranged by size: 5186 810 698 ScaI AGT'ACT Cuts at: 4942 6654 4942 Size: 1712 4982 SfcI C'TryA_G Cuts at: 826 1045 2092 2534 3344 4042 5177 5858 6049 Size: 219 1047 442 810 698 1135 681 191 Cuts at: 6049 826 Size: 1471 Fragments arranged by size: 1471 1135 1047 810 698 681 442 219 191 SfoI GGC'GCC Cuts at: 2886 2886 Size: 6694 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6694 SmaI CCC'GGG Cuts at: 1872 2790 4021 1872 Size: 918 1231 4545 Fragments arranged by size: 4545 1231 918 SmlI C'TyrA_G Cuts at: 820 1017 1943 3183 4798 5699 5961 820 Size: 197 926 1240 1615 901 262 1553 Fragments arranged by size: 1615 1553 1240 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4485 493 Size: 3992 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6694 SphI G_CATG'C Cuts at: 1132 4052 1132 Size: 2920 3774 SspI AAT'ATT Cuts at: 5 52 4618 5 Size: 47 4566 2081 Fragments arranged by size: 4566 2081 47 StuI AGG'CCT Cuts at: 1517 3129 1517 Size: 1612 5082 StyI C'CwwG_G Cuts at: 513 1065 2004 2493 2574 3116 4133 513 Size: 552 939 489 81 542 1017 3074 Fragments arranged by size: 3074 1017 939 552 542 489 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2063 2924 4721 4738 4891 5076 5521 Size: 1255 483 861 1797 17 153 185 445 Cuts at: 5521 325 Size: 1498 Fragments arranged by size: 1797 1498 1255 861 483 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4940 6652 96 Size: 274 80 33 51 1443 2963 1712 138 Fragments arranged by size: 2963 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2589 2621 3026 3058 3836 3861 3868 3893 6654 Size: 32 405 32 778 25 7 25 2761 Cuts at: 6654 6686 2589 Size: 32 2597 Fragments arranged by size: 2761 2597 778 405 32 32 32 25 25 7 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6694 XbaI T'CTAG_A Cuts at: 4030 4030 Size: 6694 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2209 2623 2815 3920 2209 Size: 414 192 1105 4983 Fragments arranged by size: 4983 1105 414 192 XhoI C'TCGA_G Cuts at: 1943 3183 1943 Size: 1240 5454 XmaI C'CCGG_G Cuts at: 1870 2788 4019 1870 Size: 918 1231 4545 Fragments arranged by size: 4545 1231 918 XmnI GAAnn'nnTTC Cuts at: 3461 4823 3461 Size: 1362 5332 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4502 276 Size: 53 83 186 936 2968 2468 Fragments arranged by size: 2968 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI PacI PsrI RsrII SexAI SfiI SrfI SwaI