Restriction map for
pFN21AB6203
(Linear) (Six-base) MAPSORT of: KIBB6203.seq Check: 5919 from: 1 to: 1989
KIBB6203.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1353 1989
Size: 1353 636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 979 1147 1678 1989
Size: 102 877 168 531 311
Fragments arranged by size:
877 531 311 168 102
AflIII A'CryG_T
Cuts at: 0 536 818 926 1146 1989
Size: 536 282 108 220 843
Fragments arranged by size:
843 536 282 220 108
AhdI GACnn_n'nnGTC
Cuts at: 0 111 1398 1989
Size: 111 1287 591
Fragments arranged by size:
1287 591 111
AleI CACnn'nnGTG
Cuts at: 0 334 1942 1989
Size: 334 1608 47
Fragments arranged by size:
1608 334 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1960 1989
Size: 1960 29
AlwNI CAG_nnn'CTG
Cuts at: 0 1338 1487 1989
Size: 1338 149 502
Fragments arranged by size:
1338 502 149
ApaI G_GGCC'C
Cuts at: 0 427 1187 1811 1989
Size: 427 760 624 178
Fragments arranged by size:
760 624 427 178
ApaLI G'TGCA_C
Cuts at: 0 1878 1927 1989
Size: 1878 49 62
Fragments arranged by size:
1878 62 49
AvaI C'yCGr_G
Cuts at: 0 783 838 1178 1989
Size: 783 55 340 811
Fragments arranged by size:
811 783 340 55
BanI G'GyrC_C
Cuts at: 0 64 439 879 1936 1983 1989
Size: 64 375 440 1057 47 6
Fragments arranged by size:
1057 440 375 64 47 6
BanII G_rGCy'C
Cuts at: 0 427 791 836 1187 1240 1811 1989
Size: 427 364 45 351 53 571 178
Fragments arranged by size:
571 427 364 351 178 53 45
BbeI G_GCGC'C
Cuts at: 0 883 1989
Size: 883 1106
BbsI GAAGACnn'nnnn_
Cuts at: 0 530 568 861 1092 1989
Size: 530 38 293 231 897
Fragments arranged by size:
897 530 293 231 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 466 500 1989
Size: 466 34 1489
Fragments arranged by size:
1489 466 34
BfrBI ATG'CAT
Cuts at: 0 1634 1989
Size: 1634 355
BglI GCCn_nnn'nGGC
Cuts at: 0 1806 1989
Size: 1806 183
Bme1580I G_kGCm'C
Cuts at: 0 67 427 1187 1359 1811 1882 1931 1988
Size: 67 360 760 172 452 71 49 57
Cuts at: 1988 1989
Size: 1
Fragments arranged by size:
760 452 360 172 71 67 57 49
1
BmgBI CAC'GTC
Cuts at: 0 113 821 1989
Size: 113 708 1168
Fragments arranged by size:
1168 708 113
BmrI ACTGGGnnnn_n'
Cuts at: 0 1237 1876 1989
Size: 1237 639 113
Fragments arranged by size:
1237 639 113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 490 1989
Size: 490 1499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 850 1855 1858 1879 1989
Size: 152 698 1005 3 21 110
Fragments arranged by size:
1005 698 152 110 21 3
Bpu10I CC'TnA_GC
Cuts at: 0 553 1426 1989
Size: 553 873 563
Fragments arranged by size:
873 563 553
BsaAI yAC'GTr
Cuts at: 0 606 750 1413 1989
Size: 606 144 663 576
Fragments arranged by size:
663 606 576 144
BsaHI Gr'CG_yC
Cuts at: 0 880 1989
Size: 880 1109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 584 614 1831 1856 1861 1886 1989
Size: 584 30 1217 25 5 25 103
Fragments arranged by size:
1217 584 103 30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1030 1033 1852 1989
Size: 1030 3 819 137
Fragments arranged by size:
1030 819 137 3
BseYI C'CCAG_C
Cuts at: 0 60 905 1272 1306 1358 1668 1989
Size: 60 845 367 34 52 310 321
Fragments arranged by size:
845 367 321 310 60 52 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 693 951 1387 1989
Size: 93 600 258 436 602
Fragments arranged by size:
602 600 436 258 93
BsiEI CG_ry'CG
Cuts at: 0 1824 1989
Size: 1824 165
BsiHKAI G_wGCw'C
Cuts at: 0 559 602 836 1882 1931 1989
Size: 559 43 234 1046 49 58
Fragments arranged by size:
1046 559 234 58 49 43
Bsp1286I G_dGCh'C
Cuts at: 0 67 427 559 602 791 836 1187 1240
Size: 67 360 132 43 189 45 351 53
Cuts at: 1240 1359 1811 1882 1931 1988 1989
Size: 119 452 71 49 57 1
Fragments arranged by size:
452 360 351 189 132 119 71 67
57 53 49 45 43 1
BspHI T'CATG_A
Cuts at: 0 890 1989
Size: 890 1099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 1353 1989
Size: 536 817 636
Fragments arranged by size:
817 636 536
BsrDI GCAATG_nn'
Cuts at: 0 1317 1989
Size: 1317 672
BsrFI r'CCGG_y
Cuts at: 0 370 1319 1989
Size: 370 949 670
Fragments arranged by size:
949 670 370
BstBI TT'CG_AA
Cuts at: 0 1544 1989
Size: 1544 445
BstEII G'GTnAC_C
Cuts at: 0 225 1989
Size: 225 1764
BstXI CCAn_nnnn'nTGG
Cuts at: 0 450 1989
Size: 450 1539
Bsu36I CC'TnA_GG
Cuts at: 0 101 1013 1989
Size: 101 912 976
Fragments arranged by size:
976 912 101
BtgI C'CryG_G
Cuts at: 0 332 488 1094 1403 1524 1989
Size: 332 156 606 309 121 465
Fragments arranged by size:
606 465 332 309 156 121
BtsI GCAGTG_nn'
Cuts at: 0 1929 1989
Size: 1929 60
DrdI GACnn_nn'nnGTC
Cuts at: 0 865 1989
Size: 865 1124
EaeI y'GGCC_r
Cuts at: 0 245 608 977 1472 1676 1821 1989
Size: 245 363 369 495 204 145 168
Fragments arranged by size:
495 369 363 245 204 168 145
EagI C'GGCC_G
Cuts at: 0 1821 1989
Size: 1821 168
EarI CTCTTCn'nnn_
Cuts at: 0 1291 1714 1989
Size: 1291 423 275
Fragments arranged by size:
1291 423 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1750 1989
Size: 1750 239
EcoICRI GAG'CTC
Cuts at: 0 834 1989
Size: 834 1155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 152 850 979 1147 1678 1855 1858
Size: 102 50 698 129 168 531 177 3
Cuts at: 1858 1879 1989
Size: 21 110
Fragments arranged by size:
698 531 177 168 129 110 102 50
21 3
EcoO109I rG'GnC_Cy
Cuts at: 0 410 423 424 688 756 1183 1184 1335
Size: 410 13 1 264 68 427 1 151
Cuts at: 1335 1371 1484 1533 1989
Size: 36 113 49 456
Fragments arranged by size:
456 427 410 264 151 113 68 49
36 13 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 594 1989
Size: 594 1395
FspI TGC'GCA
Cuts at: 0 354 1948 1989
Size: 354 1594 41
Fragments arranged by size:
1594 354 41
HaeII r_GCGC'y
Cuts at: 0 883 1989
Size: 883 1106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1384 1416 1989
Size: 97 32 1255 32 573
Fragments arranged by size:
1255 573 97 32 32
HindIII A'AGCT_T
Cuts at: 0 1739 1989
Size: 1739 250
KasI G'GCGC_C
Cuts at: 0 879 1989
Size: 879 1110
MluI A'CGCG_T
Cuts at: 0 926 1146 1989
Size: 926 220 843
Fragments arranged by size:
926 843 220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 1989
Size: 848 1141
MscI TGG'CCA
Cuts at: 0 610 979 1474 1989
Size: 610 369 495 515
Fragments arranged by size:
610 515 495 369
MslI CAynn'nnrTG
Cuts at: 0 334 1768 1942 1989
Size: 334 1434 174 47
Fragments arranged by size:
1434 334 174 47
MspA1I CmG'CkG
Cuts at: 0 334 1272 1298 1826 1989
Size: 334 938 26 528 163
Fragments arranged by size:
938 528 334 163 26
NaeI GCC'GGC
Cuts at: 0 1321 1989
Size: 1321 668
NarI GG'CG_CC
Cuts at: 0 880 1989
Size: 880 1109
NcoI C'CATG_G
Cuts at: 0 488 1989
Size: 488 1501
NgoMIV G'CCGG_C
Cuts at: 0 1319 1989
Size: 1319 670
NsiI A_TGCA'T
Cuts at: 0 1636 1989
Size: 1636 353
NspI r_CATG'y
Cuts at: 0 540 1767 1989
Size: 540 1227 222
Fragments arranged by size:
1227 540 222
PasI CC'CwG_GG
Cuts at: 0 6 136 214 1989
Size: 6 130 78 1775
Fragments arranged by size:
1775 130 78 6
PciI A'CATG_T
Cuts at: 0 536 1989
Size: 536 1453
PflMI CCAn_nnn'nTGG
Cuts at: 0 594 1165 1989
Size: 594 571 824
Fragments arranged by size:
824 594 571
PfoI T'CCnGG_A
Cuts at: 0 767 1502 1989
Size: 767 735 487
Fragments arranged by size:
767 735 487
PmlI CAC'GTG
Cuts at: 0 606 1413 1989
Size: 606 807 576
Fragments arranged by size:
807 606 576
PpuMI rG'GwC_Cy
Cuts at: 0 410 688 1484 1989
Size: 410 278 796 505
Fragments arranged by size:
796 505 410 278
PspOMI G'GGCC_C
Cuts at: 0 423 1183 1807 1989
Size: 423 760 624 182
Fragments arranged by size:
760 624 423 182
PspXI vC'TCGA_Gb
Cuts at: 0 1178 1989
Size: 1178 811
PstI C_TGCA'G
Cuts at: 0 91 533 1343 1989
Size: 91 442 810 646
Fragments arranged by size:
810 646 442 91
PvuII CAG'CTG
Cuts at: 0 1298 1989
Size: 1298 691
SacI G_AGCT'C
Cuts at: 0 836 1989
Size: 836 1153
SacII CC_GC'GG
Cuts at: 0 335 1989
Size: 335 1654
SanDI GG'GwC_CC
Cuts at: 0 688 1989
Size: 688 1301
SapI GCTCTTCn'nnn_
Cuts at: 0 1714 1989
Size: 1714 275
SbfI CC_TGCA'GG
Cuts at: 0 533 1343 1989
Size: 533 810 646
Fragments arranged by size:
810 646 533
SfcI C'TryA_G
Cuts at: 0 87 529 1339 1989
Size: 87 442 810 650
Fragments arranged by size:
810 650 442 87
SfoI GGC'GCC
Cuts at: 0 881 1989
Size: 881 1108
SmaI CCC'GGG
Cuts at: 0 785 1989
Size: 785 1204
SmlI C'TyrA_G
Cuts at: 0 1178 1989
Size: 1178 811
StuI AGG'CCT
Cuts at: 0 1124 1989
Size: 1124 865
StyI C'CwwG_G
Cuts at: 0 488 569 1111 1989
Size: 488 81 542 878
Fragments arranged by size:
878 542 488 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 919 1989
Size: 58 861 1070
Fragments arranged by size:
1070 861 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 584 616 1021 1053 1831 1856 1863 1888
Size: 584 32 405 32 778 25 7 25
Cuts at: 1888 1989
Size: 101
Fragments arranged by size:
778 584 405 101 32 32 25 25
7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 204 618 810 1915 1989
Size: 204 414 192 1105 74
Fragments arranged by size:
1105 414 204 192 74
XhoI C'TCGA_G
Cuts at: 0 1178 1989
Size: 1178 811
XmaI C'CCGG_G
Cuts at: 0 783 1989
Size: 783 1206
XmnI GAAnn'nnTTC
Cuts at: 0 1456 1989
Size: 1456 533
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BcgI BfrBI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BsrFI BstBI BstEII BstXI Bsu36I
BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I FalI FspI HaeII Hin4I HindIII KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI
SapI SbfI SfcI SfoI SmaI SmlI StuI StyI
TaqII TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BglII BlpI BmtI BpuEI BsaI BsaBI
BsaWI BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII
BssSI BstAPI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII EcoNI EcoRI EcoRV FseI FspAI HincII HpaI
KpnI MfeI NdeI NheI NotI NruI PacI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SalI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI ZraI