Restriction map for
pFN21AB6834
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HgaI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MboII MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 28 GGCGCC G[GA]^CG[CT]C
AflIII 470 ACACGT A^C[GA][CT]GT
AspHI 559 GTGCTC G[AT]GC[AT]^C
AviII 215 TGCGCA TGC^GCA
BanI 28 GGCGCC G^G[CT][GA]CC
BbeI 28 GGCGCC GGCGC^C
BciVI 168 GGATAC GTATCC(6/5)
BsaHI 28 GGCGCC G[GA]^CG[CT]C
BsaI 427 GAGACC GGTCTC(1/5)
BsaWI 119 ACCGGA [AT]^CCGG[AT]
BseRI 184 GAGGAG GAGGAG(10/8)
BsgI 362 CTGCAC GTGCAG(16/14)
BsiHKAI 559 GTGCTC G[AT]GC[AT]^C
BsmBI 145 GAGACG CGTCTC(1/5)
BsmFI 203 GGGAC GGGAC(10/14)
BspMI 126 GCAGGT ACCTGC(4/8)
BstXI 329 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 485 AGATCC [GA]^GATC[CT]
DdeI 389 CTCAG C^T[ACGT]AG
DraII 392 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 79 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 329 CCATGG C^C[GA][CT]GG
EcoO109I 392 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 329 CCATGG C^C[AT][AT]GG
FauI 201 GCGGG CCCGC(4/6)
FspI 215 TGCGCA TGC^GCA
Hin1I 28 GGCGCC G[GA]^CG[CT]C
HphI 306 GGTGA GGTGA(8/7)
KasI 28 GGCGCC G^GCGCC
MaeIII 353 GTGAC ^GT[ACGT]AC
MflI 485 AGATCC [GA]^GATC[CT]
MslI 471 CACGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 64 CAGCTG C[AC]G^C[GT]G
NarI 28 GGCGCC GG^CGCC
NcoI 329 CCATGG C^CATGG
NspI 212 ACATGC [GA]CATG^[CT]
PflMI 78 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 313 GACTC GAGTC(4/5)
PpuMI 392 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 64 CAGCTG CAG^CTG
SfcI 104 CTATAG C^T[GA][CT]AG
SfoI 28 GGCGCC GGC^GCC
SgrAI 30 CGCCGGCG C[GA]^CCGG[CT]G
StyI 329 CCATGG C^C[AT][AT]GG
TaqI 425 TCGA T^CGA
Tsp45I 353 GTGAC ^GT[GC]AC
TspRI 369 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 425 TCGA T^CGA
Van91I 78 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 329 CCATGGTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 485 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 69 GATCC GGATC(4/5)
486 GATCC
AvaII 204 GGACC G^G[AT]CC
393 GGACC
BmyI 325 GTGCCC G[AGT]GC[ACT]^C
559 GTGCTC
BsmAI 145 GAGAC GTCTC(1/5)
427 GAGAC
Bsp1286I 325 GTGCCC G[AGT]GC[ACT]^C
559 GTGCTC
BsrFI 31 GCCGGC [GA]^CCGG[CT]
175 GCCGGC
BsrI 207 CCAGT ACTGG(1/-1)
282 CCAGT
BstAPI 495 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
542 GCATGAGCTGC
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
175 GCCGGC
EaeI 177 CGGCCA [CT]^GGCC[GA]
434 CGGCCA
HaeII 28 GGCGCC [GA]GCGC^[CT]
193 AGCGCC
NaeI 31 GCCGGC GCC^GGC
175 GCCGGC
NgoMIV 31 GCCGGC G^CCGGC
175 GCCGGC
SfaNI 460 GATGC GCATC(5/9)
413 GCATC
TfiI 112 GATTC G^A[AT]TC
376 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI