Restriction map for pFN21AB6834
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvrII       BalI        BamHI       BanII       BbrPI       
BbsI        BbvCI       BcgI        BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BseAI       BsiEI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HgaI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     KspI        MaeI        
MamI        MboII       MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tsp509I     Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcyI           28  GGCGCC               G[GA]^CG[CT]C
AflIII        470  ACACGT               A^C[GA][CT]GT
AspHI         559  GTGCTC               G[AT]GC[AT]^C
AviII         215  TGCGCA               TGC^GCA
BanI           28  GGCGCC               G^G[CT][GA]CC
BbeI           28  GGCGCC               GGCGC^C
BciVI         168  GGATAC               GTATCC(6/5)
BsaHI          28  GGCGCC               G[GA]^CG[CT]C
BsaI          427  GAGACC               GGTCTC(1/5)
BsaWI         119  ACCGGA               [AT]^CCGG[AT]
BseRI         184  GAGGAG               GAGGAG(10/8)
BsgI          362  CTGCAC               GTGCAG(16/14)
BsiHKAI       559  GTGCTC               G[AT]GC[AT]^C
BsmBI         145  GAGACG               CGTCTC(1/5)
BsmFI         203  GGGAC                GGGAC(10/14)
BspMI         126  GCAGGT               ACCTGC(4/8)
BstXI         329  CCATGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         485  AGATCC               [GA]^GATC[CT]
DdeI          389  CTCAG                C^T[ACGT]AG
DraII         392  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII         79  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          329  CCATGG               C^C[GA][CT]GG
EcoO109I      392  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       329  CCATGG               C^C[AT][AT]GG
FauI          201  GCGGG                CCCGC(4/6)
FspI          215  TGCGCA               TGC^GCA
Hin1I          28  GGCGCC               G[GA]^CG[CT]C
HphI          306  GGTGA                GGTGA(8/7)
KasI           28  GGCGCC               G^GCGCC
MaeIII        353  GTGAC                ^GT[ACGT]AC
MflI          485  AGATCC               [GA]^GATC[CT]
MslI          471  CACGTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         64  CAGCTG               C[AC]G^C[GT]G
NarI           28  GGCGCC               GG^CGCC
NcoI          329  CCATGG               C^CATGG
NspI          212  ACATGC               [GA]CATG^[CT]
PflMI          78  CCACTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          313  GACTC                GAGTC(4/5)
PpuMI         392  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII          64  CAGCTG               CAG^CTG
SfcI          104  CTATAG               C^T[GA][CT]AG
SfoI           28  GGCGCC               GGC^GCC
SgrAI          30  CGCCGGCG             C[GA]^CCGG[CT]G
StyI          329  CCATGG               C^C[AT][AT]GG
TaqI          425  TCGA                 T^CGA
Tsp45I        353  GTGAC                ^GT[GC]AC
TspRI         369  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       425  TCGA                 T^CGA
Van91I         78  CCACTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          329  CCATGGTGCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         485  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwI           69  GATCC                GGATC(4/5)
              486  GATCC               
AvaII         204  GGACC                G^G[AT]CC
              393  GGACC               
BmyI          325  GTGCCC               G[AGT]GC[ACT]^C
              559  GTGCTC              
BsmAI         145  GAGAC                GTCTC(1/5)
              427  GAGAC               
Bsp1286I      325  GTGCCC               G[AGT]GC[ACT]^C
              559  GTGCTC              
BsrFI          31  GCCGGC               [GA]^CCGG[CT]
              175  GCCGGC              
BsrI          207  CCAGT                ACTGG(1/-1)
              282  CCAGT               
BstAPI        495  GCACAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              542  GCATGAGCTGC         
Cfr10I         31  GCCGGC               [GA]^CCGG[CT]
              175  GCCGGC              
EaeI          177  CGGCCA               [CT]^GGCC[GA]
              434  CGGCCA              
HaeII          28  GGCGCC               [GA]GCGC^[CT]
              193  AGCGCC              
NaeI           31  GCCGGC               GCC^GGC
              175  GCCGGC              
NgoMIV         31  GCCGGC               G^CCGGC
              175  GCCGGC              
SfaNI         460  GATGC                GCATC(5/9)
              413  GCATC               
TfiI          112  GATTC                G^A[AT]TC
              376  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BcnI        
BsaJI       BsiYI       BslI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeII       MboI        MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       TseI        
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