(Circular) (Six-base) MAPSORT of: pFN21AB6164.seq Check: 6319 from: 1 to: 6511 pFN21AB6164 6511 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3174 4321 278 Size: 53 83 186 936 1638 1147 2468 Fragments arranged by size: 2468 1638 1147 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3018 3854 1937 Size: 1081 836 4594 Fragments arranged by size: 4594 1081 836 Acc65I G'GTAC_C Cuts at: 3832 3832 Size: 6511 AclI AA'CG_TT Cuts at: 1563 4638 5011 1563 Size: 3075 373 3063 Fragments arranged by size: 3075 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3434 3665 4574 5958 6226 1632 Size: 1802 231 909 1384 268 1917 Fragments arranged by size: 1917 1802 1384 909 268 231 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6314 AflIII A'CryG_T Cuts at: 1203 3738 5410 6387 1203 Size: 2535 1672 977 1327 Fragments arranged by size: 2535 1672 1327 977 AgeI A'CCGG_T Cuts at: 5342 5342 Size: 6511 AhdI GACnn_n'nnGTC Cuts at: 2398 5240 2398 Size: 2842 3669 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6511 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6511 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3125 3157 3198 3230 3125 Size: 32 41 32 6406 Fragments arranged by size: 6406 41 32 32 AlwNI CAG_nnn'CTG Cuts at: 2579 3442 3643 4254 5826 2579 Size: 863 201 611 1572 3264 Fragments arranged by size: 3264 1572 863 611 201 ApaI G_GGCC'C Cuts at: 2238 2238 Size: 6511 ApaLI G'TGCA_C Cuts at: 4568 5724 4568 Size: 1156 5355 ApoI r'AATT_y Cuts at: 1493 2423 3820 4046 4198 6307 1493 Size: 930 1397 226 152 2109 1697 Fragments arranged by size: 2109 1697 1397 930 226 152 AseI AT'TA_AT Cuts at: 160 1032 5065 160 Size: 872 4033 1606 Fragments arranged by size: 4033 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 AvaI C'yCGr_G Cuts at: 1870 1943 2074 2760 3324 3836 1870 Size: 73 131 686 564 512 4545 Fragments arranged by size: 4545 686 564 512 131 73 BamHI G'GATC_C Cuts at: 2755 3841 4208 2755 Size: 1086 367 5058 Fragments arranged by size: 5058 1086 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3832 5287 618 Size: 325 217 627 2045 1455 1842 Fragments arranged by size: 2045 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 2238 2607 2820 2904 3577 3830 1391 Size: 646 201 369 213 84 673 253 4072 Fragments arranged by size: 4072 673 646 369 253 213 201 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5540 BbvCI CC'TCA_GC Cuts at: 2285 3027 3627 2285 Size: 742 600 5169 Fragments arranged by size: 5169 742 600 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4702 4736 1903 Size: 34 50 34 2681 34 3678 Fragments arranged by size: 3678 2681 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3570 4403 5613 3570 Size: 833 1210 4468 Fragments arranged by size: 4468 1210 833 BclI T'GATC_A Cuts at: 1548 1548 Size: 6511 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5122 136 Size: 107 122 71 631 605 3450 1525 Fragments arranged by size: 3450 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6506 6506 Size: 6511 BlpI GC'TnA_GC Cuts at: 2210 2898 3928 2210 Size: 688 1030 4793 Fragments arranged by size: 4793 1030 688 Bme1580I G_kGCm'C Cuts at: 1790 2238 4572 5728 1790 Size: 448 2334 1156 2573 Fragments arranged by size: 2573 2334 1156 448 BmgBI CAC'GTC Cuts at: 1318 3739 1318 Size: 2421 4090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2143 3550 4242 Size: 416 53 83 399 24 726 1407 692 Cuts at: 4242 5200 442 Size: 958 1753 Fragments arranged by size: 1753 1407 958 726 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2618 2927 3077 3176 3779 5171 1239 Size: 1379 309 150 99 603 1392 2579 Fragments arranged by size: 2579 1392 1379 603 309 150 99 Bpu10I CC'TnA_GC Cuts at: 2285 3027 3627 2285 Size: 742 600 5169 Fragments arranged by size: 5169 742 600 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2687 2762 3086 4636 5501 5799 2687 Size: 75 324 1550 865 298 3399 Fragments arranged by size: 3399 1550 865 324 298 75 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3608 5174 882 Size: 617 2109 1566 2219 Fragments arranged by size: 2219 2109 1566 617 BsaAI yAC'GTr Cuts at: 493 1204 4302 493 Size: 711 3098 2702 Fragments arranged by size: 3098 2702 711 BsaBI GATnn'nnATC Cuts at: 6505 6505 Size: 6511 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3171 4318 4700 5335 Size: 53 83 186 936 1638 1147 382 635 Cuts at: 5335 275 Size: 1451 Fragments arranged by size: 1638 1451 1147 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2544 4944 5342 5616 5763 6174 2544 Size: 2400 398 274 147 411 2881 Fragments arranged by size: 2881 2400 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2339 2369 2715 2745 3125 Size: 30 165 30 936 30 346 30 380 Cuts at: 3125 3155 3347 3377 1178 Size: 30 192 30 4312 Fragments arranged by size: 4312 936 380 346 192 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2260 2363 3173 3548 1186 Size: 934 140 103 810 375 4149 Fragments arranged by size: 4149 934 810 375 140 103 BseYI C'CCAG_C Cuts at: 2112 2124 2130 2136 2180 5714 6210 2112 Size: 12 6 6 44 3534 496 2413 Fragments arranged by size: 3534 2413 496 44 12 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3333 3439 1163 Size: 573 1597 106 4235 Fragments arranged by size: 4235 1597 573 106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3961 4722 5750 1540 Size: 275 186 1960 761 1028 2301 Fragments arranged by size: 2301 1960 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2904 2961 3271 4572 4657 5728 2904 Size: 57 310 1301 85 1071 3687 Fragments arranged by size: 3687 1301 1071 310 85 57 BsmI GAATG_Cn' Cuts at: 4022 4115 4022 Size: 93 6418 BsmBI CGTCTCn'nnnn_ Cuts at: 2867 2867 Size: 6511 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2238 2607 2820 2904 2961 3271 Size: 399 247 201 369 213 84 57 310 Cuts at: 3271 3577 3830 4572 4657 5728 1391 Size: 306 253 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 310 306 253 247 213 201 85 84 57 BspEI T'CCGG_A Cuts at: 2544 6174 2544 Size: 3630 2881 BspHI T'CATG_A Cuts at: 3297 4400 6130 3297 Size: 1103 1730 3678 Fragments arranged by size: 3678 1730 1103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3866 844 Size: 3022 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2344 2767 4398 1394 Size: 429 521 423 1631 3507 Fragments arranged by size: 3507 1631 521 429 423 BsrDI GCAATG_nn' Cuts at: 65 1248 5006 5180 65 Size: 1183 3758 174 1396 Fragments arranged by size: 3758 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3082 3489 5155 5342 1700 Size: 110 1272 407 1666 187 2869 Fragments arranged by size: 2869 1666 1272 407 187 110 BsrGI T'GTAC_A Cuts at: 96 3741 96 Size: 3645 2866 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6511 BssSI C'ACGA_G Cuts at: 2639 4571 5583 2639 Size: 1932 1012 3567 Fragments arranged by size: 3567 1932 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3266 794 Size: 2472 4039 BstBI TT'CG_AA Cuts at: 5315 5315 Size: 6511 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6511 BstYI r'GATC_y Cuts at: 1972 2755 2910 3841 4208 4593 4610 6062 6148 Size: 783 155 931 367 385 17 1452 86 Cuts at: 6148 6160 6506 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 931 783 385 367 346 155 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2511 2511 Size: 6511 BtgI C'CryG_G Cuts at: 513 1065 3391 513 Size: 552 2326 3633 Fragments arranged by size: 3633 2326 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2777 487 Size: 683 56 1551 4221 Fragments arranged by size: 4221 1551 683 56 BtsI GCAGTG_nn' Cuts at: 3584 4027 4821 4841 3584 Size: 443 794 20 5254 Fragments arranged by size: 5254 794 443 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6511 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2068 2103 529 Size: 35 1504 35 4937 Fragments arranged by size: 4937 1504 35 35 DraI TTT'AAA Cuts at: 3815 3815 Size: 6511 DraIII CAC_nnn'GTG Cuts at: 2414 2414 Size: 6511 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5518 809 Size: 507 4202 1802 Fragments arranged by size: 4202 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2624 3225 3958 Size: 54 1425 50 275 18 794 601 733 Cuts at: 3958 4847 6476 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 794 733 601 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3958 1537 Size: 275 2146 4090 Fragments arranged by size: 4090 2146 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2546 2949 3218 3507 3641 3774 4441 1353 Size: 1193 403 269 289 134 133 667 3423 Fragments arranged by size: 3423 1193 667 403 289 269 134 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3206 3791 5098 5472 5618 387 Size: 1064 138 1617 585 1307 374 146 1280 Fragments arranged by size: 1617 1307 1280 1064 585 374 146 138 EcoICRI GAG'CTC Cuts at: 2902 2902 Size: 6511 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2618 2927 3077 3176 3434 3665 3779 Size: 393 986 309 150 99 258 231 114 Cuts at: 3779 4574 5171 5958 6226 1239 Size: 795 597 787 268 1524 Fragments arranged by size: 1524 986 795 787 597 393 309 268 258 231 150 114 99 EcoNI CCTnn'n_nnAGG Cuts at: 2996 3059 2996 Size: 63 6448 EcoO109I rG'GnC_Cy Cuts at: 2234 2235 2234 Size: 1 6510 EcoRI G'AATT_C Cuts at: 3820 4198 3820 Size: 378 6133 FspI TGC'GCA Cuts at: 5017 5017 Size: 6511 HaeII r_GCGC'y Cuts at: 1678 5658 1678 Size: 3980 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 2866 2898 5152 Size: 32 135 32 812 32 450 32 2254 Cuts at: 5152 5184 5226 5258 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2254 812 450 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3855 4101 669 Size: 988 281 1917 246 3079 Fragments arranged by size: 3079 1917 988 281 246 HindIII A'AGCT_T Cuts at: 748 2309 748 Size: 1561 4950 HpaI GTT'AAC Cuts at: 4101 4101 Size: 6511 KpnI G_GTAC'C Cuts at: 3836 3836 Size: 6511 MfeI C'AATT_G Cuts at: 4110 4110 Size: 6511 MluI A'CGCG_T Cuts at: 6387 6387 Size: 6511 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 3702 5625 5809 1305 Size: 888 1509 1923 184 2007 Fragments arranged by size: 2007 1923 1509 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2626 3227 10 Size: 54 1425 343 794 601 3294 Fragments arranged by size: 3294 1425 794 601 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 4469 4828 4987 518 Size: 648 1315 1988 359 159 2042 Fragments arranged by size: 2042 1988 1315 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3031 3223 3626 3927 4604 5752 5997 1606 Size: 1425 192 403 301 677 1148 245 2120 Fragments arranged by size: 2120 1425 1148 677 403 301 245 192 NcoI C'CATG_G Cuts at: 513 1065 3391 513 Size: 552 2326 3633 Fragments arranged by size: 3633 2326 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6511 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6511 NotI GC'GGCC_GC Cuts at: 3958 3958 Size: 6511 NspI r_CATG'y Cuts at: 1132 1229 3869 5414 1132 Size: 97 2640 1545 2229 Fragments arranged by size: 2640 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6511 PciI A'CATG_T Cuts at: 5410 5410 Size: 6511 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6511 PfoI T'CCnGG_A Cuts at: 2904 3054 2904 Size: 150 6361 PmeI GTTT'AAAC Cuts at: 3815 3815 Size: 6511 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3125 3157 4580 4612 6093 6125 3125 Size: 32 1423 32 1481 32 3511 Fragments arranged by size: 3511 1481 1423 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6511 PsiI TTA'TAA Cuts at: 4081 4081 Size: 6511 PspOMI G'GGCC_C Cuts at: 2234 2234 Size: 6511 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3275 3307 3551 3583 3275 Size: 32 244 32 6203 Fragments arranged by size: 6203 244 32 32 PstI C_TGCA'G Cuts at: 830 2307 3863 830 Size: 1477 1556 3478 Fragments arranged by size: 3478 1556 1477 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6511 PvuII CAG'CTG Cuts at: 3031 3223 3626 3031 Size: 192 403 5916 Fragments arranged by size: 5916 403 192 SacI G_AGCT'C Cuts at: 2904 2904 Size: 6511 SalI G'TCGA_C Cuts at: 1936 3853 1936 Size: 1917 4594 SapI GCTCTTCn'nnn_ Cuts at: 2949 2949 Size: 6511 SbfI CC_TGCA'GG Cuts at: 3863 3863 Size: 6511 ScaI AGT'ACT Cuts at: 2225 4759 6471 2225 Size: 2534 1712 2265 Fragments arranged by size: 2534 2265 1712 SfcI C'TryA_G Cuts at: 826 1045 2303 2857 3355 3859 4994 5675 5866 Size: 219 1258 554 498 504 1135 681 191 Cuts at: 5866 826 Size: 1471 Fragments arranged by size: 1471 1258 1135 681 554 504 498 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6511 SmaI CCC'GGG Cuts at: 1872 3838 1872 Size: 1966 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 2760 2777 3101 4615 5516 Size: 197 926 723 94 17 324 1514 901 Cuts at: 5516 5778 820 Size: 262 1553 Fragments arranged by size: 1553 1514 926 901 723 324 262 197 94 17 SnaBI TAC'GTA Cuts at: 493 4302 493 Size: 3809 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6511 SphI G_CATG'C Cuts at: 1132 3869 1132 Size: 2737 3774 SspI AAT'ATT Cuts at: 5 52 4435 5 Size: 47 4383 2081 Fragments arranged by size: 4383 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6511 StyI C'CwwG_G Cuts at: 513 1065 2040 3129 3391 3950 513 Size: 552 975 1089 262 559 3074 Fragments arranged by size: 3074 1089 975 559 552 262 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2489 4538 4555 4708 4893 5338 325 Size: 1255 909 2049 17 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 909 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 2466 2703 Size: 274 80 33 51 1443 246 243 237 Cuts at: 2703 2722 3291 3597 3741 4757 6469 96 Size: 19 569 306 144 1016 1712 138 Fragments arranged by size: 1712 1443 1016 569 306 274 246 243 237 144 138 80 51 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3503 3535 6471 6503 3503 Size: 32 2936 32 3511 Fragments arranged by size: 3511 2936 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6511 XbaI T'CTAG_A Cuts at: 3847 3847 Size: 6511 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3754 3754 Size: 6511 XhoI C'TCGA_G Cuts at: 1943 2760 1943 Size: 817 5694 XmaI C'CCGG_G Cuts at: 1870 3836 1870 Size: 1966 4545 XmnI GAAnn'nnTTC Cuts at: 2540 4640 2540 Size: 2100 4411 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3172 4319 276 Size: 53 83 186 936 1638 1147 2468 Fragments arranged by size: 2468 1638 1147 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI