(Circular) (Six-base) MAPSORT of: pFN21AB6292.seq Check: 3852 from: 1 to: 7072 pFN21AB6292 7072 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3756 3852 3756 Size: 96 6976 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4882 278 Size: 53 83 186 936 3346 2468 Fragments arranged by size: 3346 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3234 4415 1937 Size: 1297 1181 4594 Fragments arranged by size: 4594 1297 1181 Acc65I G'GTAC_C Cuts at: 4327 4393 4327 Size: 66 7006 AclI AA'CG_TT Cuts at: 1563 3630 5199 5572 1563 Size: 2067 1569 373 3063 Fragments arranged by size: 3063 2067 1569 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3614 3686 5135 6519 6787 1632 Size: 1982 72 1449 1384 268 1917 Fragments arranged by size: 1982 1917 1449 1384 268 72 AfeI AGC'GCT Cuts at: 3175 3175 Size: 7072 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6875 AflIII A'CryG_T Cuts at: 1203 2228 3030 3475 5971 6948 1203 Size: 1025 802 445 2496 977 1327 Fragments arranged by size: 2496 1327 1025 977 802 445 AgeI A'CCGG_T Cuts at: 5903 5903 Size: 7072 AhdI GACnn_n'nnGTC Cuts at: 5801 5801 Size: 7072 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7072 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2387 1184 Size: 1203 5869 AlwNI CAG_nnn'CTG Cuts at: 2051 4815 6387 2051 Size: 2764 1572 2736 Fragments arranged by size: 2764 2736 1572 ApaI G_GGCC'C Cuts at: 2806 2990 2806 Size: 184 6888 ApaLI G'TGCA_C Cuts at: 3683 3911 5129 6285 3683 Size: 228 1218 1156 4470 Fragments arranged by size: 4470 1218 1156 228 ApoI r'AATT_y Cuts at: 1493 4170 4381 4607 4759 6868 1493 Size: 2677 211 226 152 2109 1697 Fragments arranged by size: 2677 2109 1697 226 211 152 AseI AT'TA_AT Cuts at: 160 1032 5626 160 Size: 872 4594 1606 Fragments arranged by size: 4594 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7072 AvaI C'yCGr_G Cuts at: 1870 1943 2400 2781 3217 3295 3942 4121 4397 Size: 73 457 381 436 78 647 179 276 Cuts at: 4397 1870 Size: 4545 Fragments arranged by size: 4545 647 457 436 381 276 179 78 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2109 2142 2109 Size: 33 7039 BamHI G'GATC_C Cuts at: 4366 4402 4769 4366 Size: 36 367 6669 Fragments arranged by size: 6669 367 36 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3367 4039 4327 4393 5848 Size: 325 217 627 1580 672 288 66 1455 Cuts at: 5848 618 Size: 1842 Fragments arranged by size: 1842 1580 1455 672 627 325 288 217 66 BanII G_rGCy'C Cuts at: 1391 2762 2806 2945 2990 3059 3302 3322 4391 Size: 1371 44 139 45 69 243 20 1069 Cuts at: 4391 1391 Size: 4072 Fragments arranged by size: 4072 1371 1069 243 139 69 45 44 20 BbeI G_GCGC'C Cuts at: 3371 4043 3371 Size: 672 6400 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2755 2823 928 Size: 971 856 68 5177 Fragments arranged by size: 5177 971 856 68 BbvCI CC'TCA_GC Cuts at: 3499 3499 Size: 7072 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3229 3263 3286 3320 5263 5297 1903 Size: 34 1292 34 23 34 1943 34 3678 Fragments arranged by size: 3678 1943 1292 34 34 34 34 23 BciVI GTATCCnnnnn_n' Cuts at: 4964 6174 4964 Size: 1210 5862 BclI T'GATC_A Cuts at: 1548 1548 Size: 7072 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3467 5683 136 Size: 107 122 71 631 605 1795 2216 1525 Fragments arranged by size: 2216 1795 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4060 4282 7067 4060 Size: 222 2785 4065 Fragments arranged by size: 4065 2785 222 BlpI GC'TnA_GC Cuts at: 4489 4489 Size: 7072 Bme1580I G_kGCm'C Cuts at: 1790 2228 2806 2990 3230 3687 3890 3915 5133 Size: 438 578 184 240 457 203 25 1218 Cuts at: 5133 6289 1790 Size: 1156 2573 Fragments arranged by size: 2573 1218 1156 578 457 438 240 203 184 25 BmgBI CAC'GTC Cuts at: 1318 2753 4129 1318 Size: 1435 1376 4261 Fragments arranged by size: 4261 1435 1376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3898 3961 4803 Size: 416 53 83 399 24 2481 63 842 Cuts at: 4803 5761 442 Size: 958 1753 Fragments arranged by size: 2481 1753 958 842 416 399 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7072 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2929 3317 3879 5732 1239 Size: 1690 388 562 1853 2579 Fragments arranged by size: 2579 1853 1690 562 388 Bpu10I CC'TnA_GC Cuts at: 3170 3499 3170 Size: 329 6743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2921 2982 3818 3986 5197 6062 6360 2921 Size: 61 836 168 1211 865 298 3633 Fragments arranged by size: 3633 1211 865 836 298 168 61 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2848 3447 5735 882 Size: 617 1349 599 2288 2219 Fragments arranged by size: 2288 2219 1349 617 599 BsaAI yAC'GTr Cuts at: 493 1204 2862 4863 493 Size: 711 1658 2001 2702 Fragments arranged by size: 2702 2001 1658 711 BsaBI GATnn'nnATC Cuts at: 2438 7066 2438 Size: 4628 2444 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3368 4040 4879 5261 Size: 53 83 186 936 1835 672 839 382 Cuts at: 5261 5896 275 Size: 635 1451 Fragments arranged by size: 1835 1451 936 839 672 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5505 5903 6177 6324 6735 5505 Size: 398 274 147 411 5842 Fragments arranged by size: 5842 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2996 3026 3947 3977 1178 Size: 30 165 30 1593 30 921 30 4273 Fragments arranged by size: 4273 1593 921 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3236 4238 4243 1186 Size: 2050 1002 5 4015 Fragments arranged by size: 4015 2050 1002 5 BseYI C'CCAG_C Cuts at: 3518 4307 6275 6771 3518 Size: 789 1968 496 3819 Fragments arranged by size: 3819 1968 789 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3503 3908 3920 4282 1163 Size: 573 1767 405 12 362 3953 Fragments arranged by size: 3953 1767 573 405 362 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4522 5283 6311 1540 Size: 275 186 2521 761 1028 2301 Fragments arranged by size: 2521 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2594 2945 3006 3302 3687 3915 3965 5133 5218 Size: 351 61 296 385 228 50 1168 85 Cuts at: 5218 6289 2594 Size: 1071 3377 Fragments arranged by size: 3377 1168 1071 385 351 296 228 85 61 50 BsmI GAATG_Cn' Cuts at: 3629 3797 4583 4676 3629 Size: 168 786 93 6025 Fragments arranged by size: 6025 786 168 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2228 2594 2762 2806 2945 2990 3006 Size: 399 438 366 168 44 139 45 16 Cuts at: 3006 3059 3230 3302 3322 3687 3890 3915 3965 Size: 53 171 72 20 365 203 25 50 Cuts at: 3965 4391 5133 5218 6289 1391 Size: 426 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 438 426 399 366 365 203 171 168 139 85 72 53 50 45 44 25 20 16 BspEI T'CCGG_A Cuts at: 6735 6735 Size: 7072 BspHI T'CATG_A Cuts at: 4293 4961 6691 4293 Size: 668 1730 4674 Fragments arranged by size: 4674 1730 668 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3756 3852 4427 844 Size: 2912 96 575 3489 Fragments arranged by size: 3489 2912 575 96 BsrBI CCG'CTC Cuts at: 1394 1823 3949 4959 1394 Size: 429 2126 1010 3507 Fragments arranged by size: 3507 2126 1010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5567 5741 65 Size: 1183 4319 174 1396 Fragments arranged by size: 4319 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3566 4116 5716 5903 1700 Size: 110 1756 550 1600 187 2869 Fragments arranged by size: 2869 1756 1600 550 187 110 BsrGI T'GTAC_A Cuts at: 96 2069 96 Size: 1973 5099 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7072 BssSI C'ACGA_G Cuts at: 5132 6144 5132 Size: 1012 6060 BstAPI GCAn_nnn'nTGC Cuts at: 794 3633 3885 794 Size: 2839 252 3981 Fragments arranged by size: 3981 2839 252 BstBI TT'CG_AA Cuts at: 5876 5876 Size: 7072 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7072 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 7072 BstYI r'GATC_y Cuts at: 1972 2307 4060 4282 4366 4402 4769 5154 5171 Size: 335 1753 222 84 36 367 385 17 Cuts at: 5171 6623 6709 6721 7067 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1753 1452 385 367 346 335 222 86 84 36 17 12 Bsu36I CC'TnA_GG Cuts at: 4255 4255 Size: 7072 BtgI C'CryG_G Cuts at: 513 1065 3447 3465 4179 513 Size: 552 2382 18 714 3406 Fragments arranged by size: 3406 2382 714 552 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3521 487 Size: 683 56 2295 4038 Fragments arranged by size: 4038 2295 683 56 BtsI GCAGTG_nn' Cuts at: 4588 5382 5402 4588 Size: 794 20 6258 Fragments arranged by size: 6258 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7072 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7037 DraI TTT'AAA Cuts at: 4376 4376 Size: 7072 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6079 809 Size: 507 4763 1802 Fragments arranged by size: 4763 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3649 4176 4519 Size: 54 1425 50 275 18 1819 527 343 Cuts at: 4519 5408 7037 8 Size: 889 1629 43 Fragments arranged by size: 1819 1629 1425 889 527 343 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4519 1537 Size: 275 2707 4090 Fragments arranged by size: 4090 2707 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 2178 2349 3218 5002 1353 Size: 702 123 171 869 1784 3423 Fragments arranged by size: 3423 1784 869 702 171 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2147 5659 6033 6179 387 Size: 1064 138 558 3512 374 146 1280 Fragments arranged by size: 3512 1280 1064 558 374 146 138 EcoICRI GAG'CTC Cuts at: 2943 3300 2943 Size: 357 6715 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2929 3317 3614 3686 3879 5135 5732 Size: 393 1297 388 297 72 193 1256 597 Cuts at: 5732 6519 6787 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1297 1256 787 597 393 388 297 268 193 72 EcoNI CCTnn'n_nnAGG Cuts at: 2146 2146 Size: 7072 EcoO109I rG'GnC_Cy Cuts at: 2689 2731 2803 2987 2689 Size: 42 72 184 6774 Fragments arranged by size: 6774 184 72 42 EcoRI G'AATT_C Cuts at: 4381 4759 4381 Size: 378 6694 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3154 3154 Size: 7072 FspI TGC'GCA Cuts at: 5578 5578 Size: 7072 HaeII r_GCGC'y Cuts at: 1678 3177 3371 4043 6219 1678 Size: 1499 194 672 2176 2531 Fragments arranged by size: 2531 2176 1499 672 194 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5713 5745 5787 5819 1373 Size: 32 135 32 4141 32 42 32 2626 Fragments arranged by size: 4141 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4416 4662 669 Size: 988 281 2478 246 3079 Fragments arranged by size: 3079 2478 988 281 246 HindIII A'AGCT_T Cuts at: 748 2829 748 Size: 2081 4991 HpaI GTT'AAC Cuts at: 4662 4662 Size: 7072 KasI G'GCGC_C Cuts at: 3367 4039 3367 Size: 672 6400 KpnI G_GTAC'C Cuts at: 4331 4397 4331 Size: 66 7006 MfeI C'AATT_G Cuts at: 4671 4671 Size: 7072 MluI A'CGCG_T Cuts at: 3030 6948 3030 Size: 3918 3154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2694 6186 6370 1305 Size: 1389 3492 184 2007 Fragments arranged by size: 3492 2007 1389 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5250 Fragments arranged by size: 5250 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3790 3874 4145 5030 5389 5548 Size: 648 843 1781 84 271 885 359 159 Cuts at: 5548 518 Size: 2042 Fragments arranged by size: 2042 1781 885 843 648 359 271 159 84 MspA1I CmG'CkG Cuts at: 1606 3592 4488 5165 6313 6558 1606 Size: 1986 896 677 1148 245 2120 Fragments arranged by size: 2120 1986 1148 896 677 245 NarI GG'CG_CC Cuts at: 3368 4040 3368 Size: 672 6400 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6520 NdeI CA'TA_TG Cuts at: 387 387 Size: 7072 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7072 NotI GC'GGCC_GC Cuts at: 4519 4519 Size: 7072 NspI r_CATG'y Cuts at: 1132 1229 3479 4430 5975 1132 Size: 97 2250 951 1545 2229 Fragments arranged by size: 2250 2229 1545 951 97 PasI CC'CwG_GG Cuts at: 1348 2094 2963 3348 1348 Size: 746 869 385 5072 Fragments arranged by size: 5072 869 746 385 PciI A'CATG_T Cuts at: 3475 5971 3475 Size: 2496 4576 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7072 PfoI T'CCnGG_A Cuts at: 2533 2533 Size: 7072 PmeI GTTT'AAAC Cuts at: 4376 4376 Size: 7072 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5141 5173 6654 6686 5141 Size: 32 1481 32 5527 Fragments arranged by size: 5527 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2689 2731 2689 Size: 42 7030 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7072 PsiI TTA'TAA Cuts at: 4642 4642 Size: 7072 PspOMI G'GGCC_C Cuts at: 2802 2986 2802 Size: 184 6888 PspXI vC'TCGA_Gb Cuts at: 4121 4121 Size: 7072 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3020 3052 3020 Size: 32 7040 PstI C_TGCA'G Cuts at: 830 3753 4424 830 Size: 2923 671 3478 Fragments arranged by size: 3478 2923 671 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7072 SacI G_AGCT'C Cuts at: 2945 3302 2945 Size: 357 6715 SalI G'TCGA_C Cuts at: 1936 4414 1936 Size: 2478 4594 SbfI CC_TGCA'GG Cuts at: 4424 4424 Size: 7072 ScaI AGT'ACT Cuts at: 5320 7032 5320 Size: 1712 5360 SexAI A'CCwGG_T Cuts at: 3762 3762 Size: 7072 SfcI C'TryA_G Cuts at: 826 1045 3235 3424 3749 3994 4155 4420 5555 Size: 219 2190 189 325 245 161 265 1135 Cuts at: 5555 6236 6427 826 Size: 681 191 1471 Fragments arranged by size: 2190 1471 1135 681 325 265 245 219 191 189 161 SfoI GGC'GCC Cuts at: 3369 4041 3369 Size: 672 6400 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7072 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7072 SmaI CCC'GGG Cuts at: 1872 2402 3944 4399 1872 Size: 530 1542 455 4545 Fragments arranged by size: 4545 1542 530 455 SmlI C'TyrA_G Cuts at: 820 1017 1943 2936 2997 3833 4001 4121 5176 Size: 197 926 993 61 836 168 120 1055 Cuts at: 5176 6077 6339 820 Size: 901 262 1553 Fragments arranged by size: 1553 1055 993 926 901 836 262 197 168 120 61 SnaBI TAC'GTA Cuts at: 493 4863 493 Size: 4370 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7072 SphI G_CATG'C Cuts at: 1132 4430 1132 Size: 3298 3774 SspI AAT'ATT Cuts at: 5 52 2297 4996 5 Size: 47 2245 2699 2081 Fragments arranged by size: 2699 2245 2081 47 StuI AGG'CCT Cuts at: 1517 3422 1517 Size: 1905 5167 StyI C'CwwG_G Cuts at: 513 1065 2147 4053 4511 513 Size: 552 1082 1906 458 3074 Fragments arranged by size: 3074 1906 1082 552 458 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3917 5099 5116 5269 5454 5899 325 Size: 1255 2337 1182 17 153 185 445 1498 Fragments arranged by size: 2337 1498 1255 1182 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2069 2637 5318 Size: 274 80 33 51 1443 92 568 2681 Cuts at: 5318 7030 96 Size: 1712 138 Fragments arranged by size: 2681 1712 1443 568 274 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2996 3028 3947 3979 7032 7064 2996 Size: 32 919 32 3053 32 3004 Fragments arranged by size: 3053 3004 919 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7072 XbaI T'CTAG_A Cuts at: 4408 4408 Size: 7072 XhoI C'TCGA_G Cuts at: 1943 4121 1943 Size: 2178 4894 XmaI C'CCGG_G Cuts at: 1870 2400 3942 4397 1870 Size: 530 1542 455 4545 Fragments arranged by size: 4545 1542 530 455 XmnI GAAnn'nnTTC Cuts at: 2517 5201 2517 Size: 2684 4388 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4880 276 Size: 53 83 186 936 3346 2468 Fragments arranged by size: 3346 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI