Restriction map for
pFN21AB6279
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoT22I
FseI FspI HaeII HapII Hin1I
HincII HindII HpaI HpaII KasI
KspI MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1474 GGTACC G^GTACC
AclI 443 AACGTT AA^CGTT
ApaI 1357 GGGCCC GGGCC^C
AseI 1183 ATTAAT AT^TAAT
AsnI 1183 ATTAAT AT^TAAT
Asp718I 1474 GGTACC G^GTACC
AvaI 1875 CCCGAG C^[CT]CG[GA]G
BanI 1474 GGTACC G^G[CT][GA]CC
BbvCI 2143 GCTGAGG CCTCAGC(-5/-2)
BclI 1332 TGATCA T^GATCA
BseRI 250 GAGGAG GAGGAG(10/8)
BsmI 1924 GCATTC GAATGC(1/-1)
BsoBI 1875 CCCGAG C^[CT]CG[GA]G
BstAPI 1084 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1740 GGTCACC G^GT[ACGT]ACC
BstPI 1740 GGTCACC G^GT[ACGT]ACC
BstYI 167 AGATCC [GA]^GATC[CT]
CfoI 1462 GCGC GCG^C
DraII 1356 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 1939 GACAAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1538 CCACGG C^C[GA][CT]GG
EcoO109I 1356 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1740 GGTCACC G^GT[ACGT]ACC
EcoRI 1989 GAATTC G^AATTC
EcoT14I 2309 CCTTGG C^C[AT][AT]GG
FauI 451 CCCGC CCCGC(4/6)
FbaI 1332 TGATCA T^GATCA
HgaI 1141 GCGTC GACGC(5/10)
HhaI 1462 GCGC GCG^C
HinP1I 1462 GCGC G^CGC
HindIII 2165 AAGCTT A^AGCTT
KpnI 1474 GGTACC GGTAC^C
MflI 167 AGATCC [GA]^GATC[CT]
MspA1I 1508 CAGCTG C[AC]G^C[GT]G
PshBI 1183 ATTAAT AT^TAAT
Psp1406I 443 AACGTT AA^CGTT
PvuII 1508 CAGCTG CAG^CTG
SacI 2178 GAGCTC GAGCT^C
SapI 271 GAAGAGC GCTCTTC(1/4)
SmlI 302 CTCAAG C^T[CT][GA]AG
StyI 2309 CCTTGG C^C[AT][AT]GG
XcmI 1647 CCACAAAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 167 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 105 GTCTAC GT^[AC][GT]AC
412 GTAGAC
AflIII 564 ACATGT A^C[GA][CT]GT
1799 ACATGT
Asp700I 292 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1985 GAATGAATTC
AvaII 243 GGTCC G^G[AT]CC
2275 GGACC
BalI 99 TGGCCA TGG^CCA
2367 TGGCCA
BbsI 63 GTCTTC GAAGAC(2/6)
568 GTCTTC
BfaI 1706 CTAG C^TAG
1847 CTAG
BpuAI 63 GTCTTC GAAGAC(2/6)
568 GTCTTC
BsiYI 2062 CCCTTTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2206 CCCTATCATGG
BslI 2062 CCCTTTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2206 CCCTATCATGG
BspHI 840 TCATGA T^CATGA
1590 TCATGA
BspLU11I 564 ACATGT A^CATGT
1799 ACATGT
BspMI 635 ACCTGC ACCTGC(4/8)
1944 GCAGGT
BsrDI 527 GCAATG GCAATG(2/0)
1319 GCAATG
BstXI 68 CCAGGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2370 CCAGACTTTTGG
EaeI 99 TGGCCA [CT]^GGCC[GA]
2367 TGGCCA
EcoRV 222 GATATC GAT^ATC
1671 GATATC
MaeI 1706 CTAG C^TAG
1847 CTAG
MluNI 99 TGGCCA TGG^CCA
2367 TGGCCA
MscI 99 TGGCCA TGG^CCA
2367 TGGCCA
NdeI 721 CATATG CA^TATG
1247 CATATG
RcaI 840 TCATGA T^CATGA
1590 TCATGA
TaqI 147 TCGA T^CGA
1977 TCGA
TthHB8I 147 TCGA T^CGA
1977 TCGA
XmnI 292 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1985 GAATGAATTC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BanII BbvI
BmyI BpmI BsaJI BsiHKAI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI