(Circular) (Six-base) MAPSORT of: pFN21AB6279.seq Check: 692 from: 1 to: 7087 pFN21AB6279 7087 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4897 278 Size: 53 83 186 936 3361 2468 Fragments arranged by size: 3361 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2111 2418 4430 1937 Size: 174 307 2012 4594 Fragments arranged by size: 4594 2012 307 174 Acc65I G'GTAC_C Cuts at: 3479 4408 3479 Size: 929 6158 AclI AA'CG_TT Cuts at: 1563 2449 5214 5587 1563 Size: 886 2765 373 3063 Fragments arranged by size: 3063 2765 886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2630 3667 3714 3720 5150 6534 6802 1632 Size: 998 1037 47 6 1430 1384 268 1917 Fragments arranged by size: 1917 1430 1384 1037 998 268 47 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6890 AflIII A'CryG_T Cuts at: 1203 2569 3804 5986 6963 1203 Size: 1366 1235 2182 977 1327 Fragments arranged by size: 2182 1366 1327 1235 977 AgeI A'CCGG_T Cuts at: 5918 5918 Size: 7087 AhdI GACnn_n'nnGTC Cuts at: 5816 5816 Size: 7087 AleI CACnn'nnGTG Cuts at: 1166 2520 1166 Size: 1354 5733 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2550 3972 1184 Size: 1366 1422 4299 Fragments arranged by size: 4299 1422 1366 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2232 2264 2434 2466 4185 4217 2232 Size: 32 170 32 1719 32 5102 Fragments arranged by size: 5102 1719 170 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3217 3571 3634 3698 4830 6402 3217 Size: 354 63 64 1132 1572 3902 Fragments arranged by size: 3902 1572 1132 354 64 63 ApaI G_GGCC'C Cuts at: 3366 3366 Size: 7087 ApaLI G'TGCA_C Cuts at: 5144 6300 5144 Size: 1156 5931 ApoI r'AATT_y Cuts at: 1493 3724 3994 4298 4307 4396 4622 4774 6883 Size: 2231 270 304 9 89 226 152 2109 Cuts at: 6883 1493 Size: 1697 Fragments arranged by size: 2231 2109 1697 304 270 226 152 89 9 AseI AT'TA_AT Cuts at: 160 1032 3189 5641 160 Size: 872 2157 2452 1606 Fragments arranged by size: 2452 2157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 AvaI C'yCGr_G Cuts at: 1870 1943 3880 4412 1870 Size: 73 1937 532 4545 Fragments arranged by size: 4545 1937 532 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2355 2388 2621 2654 3687 3720 2355 Size: 33 233 33 1033 33 5722 Fragments arranged by size: 5722 1033 233 33 33 33 BamHI G'GATC_C Cuts at: 4417 4784 4417 Size: 367 6720 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3479 4408 5863 618 Size: 325 217 627 1692 929 1455 1842 Fragments arranged by size: 1842 1692 1455 929 627 325 217 BanII G_rGCy'C Cuts at: 1391 3131 3366 4187 4406 1391 Size: 1740 235 821 219 4072 Fragments arranged by size: 4072 1740 821 235 219 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2566 928 Size: 971 162 505 5449 Fragments arranged by size: 5449 971 505 162 BbvCI CC'TCA_GC Cuts at: 4149 4149 Size: 7087 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5278 5312 1903 Size: 34 3341 34 3678 Fragments arranged by size: 3678 3341 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4979 6189 4979 Size: 1210 5877 BclI T'GATC_A Cuts at: 1548 3337 1548 Size: 1789 5298 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5698 136 Size: 107 122 71 631 605 4026 1525 Fragments arranged by size: 4026 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7082 7082 Size: 7087 BlpI GC'TnA_GC Cuts at: 4504 4504 Size: 7087 Bme1580I G_kGCm'C Cuts at: 1790 3366 5148 6304 1790 Size: 1576 1782 1156 2573 Fragments arranged by size: 2573 1782 1576 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7087 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3360 4818 5776 Size: 416 53 83 399 24 1943 1458 958 Cuts at: 5776 442 Size: 1753 Fragments arranged by size: 1943 1753 1458 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7087 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 3239 3999 5747 1239 Size: 903 1097 760 1748 2579 Fragments arranged by size: 2579 1748 1097 903 760 Bpu10I CC'TnA_GC Cuts at: 4149 4149 Size: 7087 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2292 5212 6077 6375 2292 Size: 2920 865 298 3004 Fragments arranged by size: 3004 2920 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5750 882 Size: 617 4251 2219 Fragments arranged by size: 4251 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4878 493 Size: 711 3674 2702 Fragments arranged by size: 3674 2702 711 BsaBI GATnn'nnATC Cuts at: 7081 7081 Size: 7087 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4894 5276 5911 275 Size: 53 83 186 936 3361 382 635 1451 Fragments arranged by size: 3361 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5520 5918 6192 6339 6750 5520 Size: 398 274 147 411 5857 Fragments arranged by size: 5857 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6862 Fragments arranged by size: 6862 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 1186 Size: 1084 6003 BseYI C'CCAG_C Cuts at: 4155 4340 6290 6786 4155 Size: 185 1950 496 4456 Fragments arranged by size: 4456 1950 496 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6514 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4537 5298 6326 1540 Size: 275 186 2536 761 1028 2301 Fragments arranged by size: 2536 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2529 4187 4210 5148 5233 6304 2529 Size: 1658 23 938 85 1071 3312 Fragments arranged by size: 3312 1658 1071 938 85 23 BsmI GAATG_Cn' Cuts at: 3929 4598 4691 3929 Size: 669 93 6325 Fragments arranged by size: 6325 669 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2529 3131 3366 4187 4210 4406 5148 Size: 399 739 602 235 821 23 196 742 Cuts at: 5148 5233 6304 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 821 742 739 602 399 235 196 85 23 BspEI T'CCGG_A Cuts at: 6750 6750 Size: 7087 BspHI T'CATG_A Cuts at: 2845 3595 4976 6706 2845 Size: 750 1381 1730 3226 Fragments arranged by size: 3226 1730 1381 750 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2649 3940 4442 844 Size: 1805 1291 502 3489 Fragments arranged by size: 3489 1805 1291 502 BsrBI CCG'CTC Cuts at: 1394 1823 4974 1394 Size: 429 3151 3507 Fragments arranged by size: 3507 3151 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2539 3331 5582 5756 65 Size: 1183 1291 792 2251 174 1396 Fragments arranged by size: 2251 1396 1291 1183 792 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5731 5918 1700 Size: 110 3921 187 2869 Fragments arranged by size: 3921 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7087 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7087 BssSI C'ACGA_G Cuts at: 5147 6159 5147 Size: 1012 6075 BstAPI GCAn_nnn'nTGC Cuts at: 794 3095 794 Size: 2301 4786 BstBI TT'CG_AA Cuts at: 5891 5891 Size: 7087 BstEII G'GTnAC_C Cuts at: 1184 3745 1184 Size: 2561 4526 BstXI CCAn_nnnn'nTGG Cuts at: 2080 4382 2080 Size: 2302 4785 BstYI r'GATC_y Cuts at: 1972 2172 4417 4784 5169 5186 6638 6724 6736 Size: 200 2245 367 385 17 1452 86 12 Cuts at: 6736 7082 1972 Size: 346 1977 Fragments arranged by size: 2245 1977 1452 385 367 346 200 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3543 513 Size: 552 2478 4057 Fragments arranged by size: 4057 2478 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6348 Fragments arranged by size: 6348 683 56 BtsI GCAGTG_nn' Cuts at: 2527 2936 4251 4603 5397 5417 2527 Size: 409 1315 352 794 20 4197 Fragments arranged by size: 4197 1315 794 409 352 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7087 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7052 DraI TTT'AAA Cuts at: 4391 4391 Size: 7087 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3950 6094 809 Size: 507 2634 2144 1802 Fragments arranged by size: 2634 2144 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 4372 4534 Size: 54 1425 50 275 18 274 2268 162 Cuts at: 4534 5423 7052 8 Size: 889 1629 43 Fragments arranged by size: 2268 1629 1425 889 275 274 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4534 1537 Size: 275 2722 4090 Fragments arranged by size: 4090 2722 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 3252 3940 5017 1353 Size: 918 981 688 1077 3423 Fragments arranged by size: 3423 1077 981 918 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5674 6048 6194 387 Size: 1064 138 4085 374 146 1280 Fragments arranged by size: 4085 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4185 4185 Size: 7087 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2630 3239 3667 3714 3720 3999 Size: 393 510 488 609 428 47 6 279 Cuts at: 3999 5150 5747 6534 6802 1239 Size: 1151 597 787 268 1524 Fragments arranged by size: 1524 1151 787 609 597 510 488 428 393 279 268 47 6 EcoO109I rG'GnC_Cy Cuts at: 3362 3362 Size: 7087 EcoRI G'AATT_C Cuts at: 3994 4396 4774 3994 Size: 402 378 6307 Fragments arranged by size: 6307 402 378 EcoRV GAT'ATC Cuts at: 2229 3678 2229 Size: 1449 5638 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2900 4045 2900 Size: 1145 5942 FspI TGC'GCA Cuts at: 5593 5593 Size: 7087 HaeII r_GCGC'y Cuts at: 1678 6234 1678 Size: 4556 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2471 2503 2856 Size: 32 135 32 641 32 226 32 353 Cuts at: 2856 2888 2957 2989 5728 5760 5802 5834 1373 Size: 32 69 32 2739 32 42 32 2626 Fragments arranged by size: 2739 2626 641 353 226 135 69 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4431 4677 669 Size: 988 281 2493 246 3079 Fragments arranged by size: 3079 2493 988 281 246 HindIII A'AGCT_T Cuts at: 748 4170 748 Size: 3422 3665 HpaI GTT'AAC Cuts at: 4677 4677 Size: 7087 KpnI G_GTAC'C Cuts at: 3483 4412 3483 Size: 929 6158 MfeI C'AATT_G Cuts at: 4686 4686 Size: 7087 MluI A'CGCG_T Cuts at: 6963 6963 Size: 7087 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3618 3745 6201 6385 1305 Size: 2313 127 2456 184 2007 Fragments arranged by size: 2456 2313 2007 184 127 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 4374 10 Size: 54 1425 343 274 2268 2723 Fragments arranged by size: 2723 2268 1425 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 2520 2532 2586 2602 2670 5045 5404 Size: 648 1354 12 54 16 68 2375 359 Cuts at: 5404 5563 518 Size: 159 2042 Fragments arranged by size: 2375 2042 1354 648 359 159 68 54 16 12 MspA1I CmG'CkG Cuts at: 1606 3515 4503 5180 6328 6573 1606 Size: 1909 988 677 1148 245 2120 Fragments arranged by size: 2120 1909 1148 988 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6535 NdeI CA'TA_TG Cuts at: 387 2727 3253 387 Size: 2340 526 4221 Fragments arranged by size: 4221 2340 526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7087 NotI GC'GGCC_GC Cuts at: 4534 4534 Size: 7087 NspI r_CATG'y Cuts at: 1132 1229 2573 2623 3808 4445 5990 1132 Size: 97 1344 50 1185 637 1545 2229 Fragments arranged by size: 2229 1545 1344 1185 637 97 50 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7087 PciI A'CATG_T Cuts at: 2569 3804 5986 2569 Size: 1235 2182 3670 Fragments arranged by size: 3670 2182 1235 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7087 PfoI T'CCnGG_A Cuts at: 2072 2072 Size: 7087 PmeI GTTT'AAAC Cuts at: 4391 4391 Size: 7087 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5156 5188 6669 6701 5156 Size: 32 1481 32 5542 Fragments arranged by size: 5542 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7087 PsiI TTA'TAA Cuts at: 4657 4657 Size: 7087 PspOMI G'GGCC_C Cuts at: 3362 3362 Size: 7087 PstI C_TGCA'G Cuts at: 830 2185 3630 3834 4439 830 Size: 1355 1445 204 605 3478 Fragments arranged by size: 3478 1445 1355 605 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7087 PvuII CAG'CTG Cuts at: 3515 3515 Size: 7087 SacI G_AGCT'C Cuts at: 4187 4187 Size: 7087 SalI G'TCGA_C Cuts at: 1936 4429 1936 Size: 2493 4594 SapI GCTCTTCn'nnn_ Cuts at: 2271 2271 Size: 7087 SbfI CC_TGCA'GG Cuts at: 4439 4439 Size: 7087 ScaI AGT'ACT Cuts at: 2142 3061 3388 5335 7047 2142 Size: 919 327 1947 1712 2182 Fragments arranged by size: 2182 1947 1712 919 327 SfcI C'TryA_G Cuts at: 826 1045 2129 2181 2315 3626 3830 4435 5570 Size: 219 1084 52 134 1311 204 605 1135 Cuts at: 5570 6251 6442 826 Size: 681 191 1471 Fragments arranged by size: 1471 1311 1135 1084 681 605 219 204 191 134 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7087 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7087 SmaI CCC'GGG Cuts at: 1872 4414 1872 Size: 2542 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2307 5191 6092 6354 820 Size: 197 926 364 2884 901 262 1553 Fragments arranged by size: 2884 1553 926 901 364 262 197 SnaBI TAC'GTA Cuts at: 493 4878 493 Size: 4385 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7087 SphI G_CATG'C Cuts at: 1132 4445 1132 Size: 3313 3774 SspI AAT'ATT Cuts at: 5 52 5011 5 Size: 47 4959 2081 Fragments arranged by size: 4959 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7087 StyI C'CwwG_G Cuts at: 513 1065 4314 4526 513 Size: 552 3249 212 3074 Fragments arranged by size: 3249 3074 552 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2081 3177 5114 5131 5284 5469 5914 Size: 1255 501 1096 1937 17 153 185 445 Cuts at: 5914 325 Size: 1498 Fragments arranged by size: 1937 1498 1255 1096 501 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2140 3059 3386 Size: 274 80 33 51 1443 163 919 327 Cuts at: 3386 5333 7045 96 Size: 1947 1712 138 Fragments arranged by size: 1947 1712 1443 919 327 274 163 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2316 2348 7047 7079 2316 Size: 32 4699 32 2324 Fragments arranged by size: 4699 2324 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7087 XbaI T'CTAG_A Cuts at: 4423 4423 Size: 7087 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3659 3659 Size: 7087 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7087 XmaI C'CCGG_G Cuts at: 1870 4412 1870 Size: 2542 4545 XmnI GAAnn'nnTTC Cuts at: 2301 3994 5216 2301 Size: 1693 1222 4172 Fragments arranged by size: 4172 1693 1222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4895 276 Size: 53 83 186 936 3361 2468 Fragments arranged by size: 3361 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI