Restriction map for
pFN21AB6272
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccII AcsI
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbeI BbrPI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I CelII
CfoI ClaI CpoI DraIII DrdI
Eco47III EcoO65I EcoRI FbaI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI MvnI
NarI NciI NdeI NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 1505 TCCGGA T^CCGGA
AclI 1088 AACGTT AA^CGTT
AhdI 556 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2133 GATCC GGATC(4/5)
AlwNI 1062 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 332 CCTTAGG CC^T[ACGT]AGG
ApaI 1521 GGGCCC GGGCC^C
Asp700I 716 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 556 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 33 GGTGCC G^G[CT][GA]CC
BbvCI 1112 CCTCAGC CCTCAGC(-5/-2)
BcgI 1285 GCACTTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1948 AGATCT A^GATCT
BsaBI 961 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1505 TCCGGA T^CCGGA
BsiEI 2079 CGGCCG CG[GA][CT]^CG
BspEI 1505 TCCGGA T^CCGGA
BspMI 1871 ACCTGC ACCTGC(4/8)
BsrBI 2008 GAGCGG CCGCTC(-3/-3)
BstAPI 871 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 824 TTCGAA TT^CGAA
BstYI 1948 AGATCT [GA]^GATC[CT]
Bsu36I 332 CCTTAGG CC^T[ACGT]AGG
DraI 118 TTTAAA TTT^AAA
EagI 2079 CGGCCG C^GGCCG
Eam1105I 556 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2079 CGGCCG C^GGCCG
Eco52I 2079 CGGCCG C^GGCCG
Eco81I 332 CCTTAGG CC^T[ACGT]AGG
EcoRV 1924 GATATC GAT^ATC
FseI 2268 GGCCGGCC GGCCGG^CC
MaeII 1089 ACGT A^CGT
MamI 961 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1948 AGATCT [GA]^GATC[CT]
MroI 1505 TCCGGA T^CCGGA
MseI 119 TTAA T^TAA
MspA1I 1382 CAGCTG C[AC]G^C[GT]G
NspV 824 TTCGAA TT^CGAA
PaeR7I 168 CTCGAG C^TCGAG
Psp1406I 1088 AACGTT AA^CGTT
PvuII 1382 CAGCTG CAG^CTG
ScaI 746 AGTACT AGT^ACT
SfuI 824 TTCGAA TT^CGAA
SphI 1793 GCATGC GCATG^C
Tru9I 119 TTAA T^TAA
XbaI 1393 TCTAGA T^CTAGA
XhoI 168 CTCGAG C^TCGAG
XhoII 1948 AGATCT [GA]^GATC[CT]
XmnI 716 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspHI 987 GAGCTC G[AT]GC[AT]^C
2074 GAGCTC
AvaI 168 CTCGAG C^[CT]CG[GA]G
2030 CCCGAG
BalI 963 TGGCCA TGG^CCA
2018 TGGCCA
BbsI 457 GAAGAC GAAGAC(2/6)
1133 GTCTTC
BpuAI 457 GAAGAC GAAGAC(2/6)
1133 GTCTTC
BsaWI 1505 TCCGGA [AT]^CCGG[AT]
1722 TCCGGT
BsgI 92 GTGCAG GTGCAG(16/14)
2089 GTGCAG
BsiHKAI 987 GAGCTC G[AT]GC[AT]^C
2074 GAGCTC
BsmBI 1418 CGTCTC CGTCTC(1/5)
2291 GAGACG
BsoBI 168 CTCGAG C^[CT]CG[GA]G
2030 CCCGAG
BstXI 266 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1155 CCATCGTAGTGG
DsaI 800 CCATGG C^C[GA][CT]GG
1592 CCATGG
EcoNI 1783 CCTTACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2136 CCTCCACCAGG
EcoT22I 307 ATGCAT ATGCA^T
1558 ATGCAT
FauI 1241 GCGGG CCCGC(4/6)
2043 GCGGG
HgaI 2293 GACGC GACGC(5/10)
2037 GCGTC
MluNI 963 TGGCCA TGG^CCA
2018 TGGCCA
MscI 963 TGGCCA TGG^CCA
2018 TGGCCA
NaeI 1249 GCCGGC GCC^GGC
2269 GCCGGC
NcoI 800 CCATGG C^CATGG
1592 CCATGG
NgoMIV 1249 GCCGGC G^CCGGC
2269 GCCGGC
NsiI 307 ATGCAT ATGCA^T
1558 ATGCAT
SacI 987 GAGCTC GAGCT^C
2074 GAGCTC
SexAI 735 ACCTGGT A^CC[AT]GGT
1715 ACCAGGT
SfcI 1068 CTGTAG C^T[GA][CT]AG
1807 CTACAG
SmlI 168 CTCGAG C^T[CT][GA]AG
2101 CTCAAG
StuI 781 AGGCCT AGG^CCT
1409 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI