(Circular) (Six-base) MAPSORT of: pFN21AB6272.seq Check: 9679 from: 1 to: 7024 pFN21AB6272 7024 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4834 278 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4367 1937 Size: 2430 4594 Acc65I G'GTAC_C Cuts at: 4345 4345 Size: 7024 AclI AA'CG_TT Cuts at: 1563 3094 5151 5524 1563 Size: 1531 2057 373 3063 Fragments arranged by size: 3063 2057 1531 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2841 5087 6471 6739 1632 Size: 1209 2246 1384 268 1917 Fragments arranged by size: 2246 1917 1384 1209 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6827 AflIII A'CryG_T Cuts at: 1203 5923 6900 1203 Size: 4720 977 1327 Fragments arranged by size: 4720 1327 977 AgeI A'CCGG_T Cuts at: 5855 5855 Size: 7024 AhdI GACnn_n'nnGTC Cuts at: 2566 5753 2566 Size: 3187 3837 AleI CACnn'nnGTG Cuts at: 1166 2799 2979 1166 Size: 1633 180 5211 Fragments arranged by size: 5211 1633 180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7024 AlwNI CAG_nnn'CTG Cuts at: 3072 4767 6339 3072 Size: 1695 1572 3757 Fragments arranged by size: 3757 1695 1572 ApaI G_GGCC'C Cuts at: 3530 3530 Size: 7024 ApaLI G'TGCA_C Cuts at: 5081 6237 5081 Size: 1156 5868 ApoI r'AATT_y Cuts at: 1493 4333 4559 4711 6820 1493 Size: 2840 226 152 2109 1697 Fragments arranged by size: 2840 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5578 160 Size: 872 4546 1606 Fragments arranged by size: 4546 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 AvaI C'yCGr_G Cuts at: 1870 1943 2173 4035 4349 1870 Size: 73 230 1862 314 4545 Fragments arranged by size: 4545 1862 314 230 73 BamHI G'GATC_C Cuts at: 4354 4721 4354 Size: 367 6657 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 4345 5800 618 Size: 325 217 627 251 2307 1455 1842 Fragments arranged by size: 2307 1842 1455 627 325 251 217 BanII G_rGCy'C Cuts at: 1391 2633 2996 3530 3614 4083 4343 1391 Size: 1242 363 534 84 469 260 4072 Fragments arranged by size: 4072 1242 534 469 363 260 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2469 3131 928 Size: 971 570 662 4821 Fragments arranged by size: 4821 971 662 570 BbvCI CC'TCA_GC Cuts at: 3118 3118 Size: 7024 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3279 3313 5215 5249 1903 Size: 34 1342 34 1902 34 3678 Fragments arranged by size: 3678 1902 1342 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4916 6126 4916 Size: 1210 5814 BclI T'GATC_A Cuts at: 1548 1548 Size: 7024 BfrBI ATG'CAT Cuts at: 2314 3565 2314 Size: 1251 5773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5635 136 Size: 107 122 71 631 605 3963 1525 Fragments arranged by size: 3963 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3953 7019 3953 Size: 3066 3958 BlpI GC'TnA_GC Cuts at: 4441 4441 Size: 7024 Bme1580I G_kGCm'C Cuts at: 1790 3530 5085 6241 1790 Size: 1740 1555 1156 2573 Fragments arranged by size: 2573 1740 1555 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7024 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2743 4755 5713 Size: 416 53 83 399 24 1326 2012 958 Cuts at: 5713 442 Size: 1753 Fragments arranged by size: 2012 1753 1326 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7024 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2167 2826 2983 3291 3294 4193 5684 Size: 803 125 659 157 308 3 899 1491 Cuts at: 5684 1239 Size: 2579 Fragments arranged by size: 2579 1491 899 803 659 308 157 125 3 Bpu10I CC'TnA_GC Cuts at: 2476 3118 3613 2476 Size: 642 495 5887 Fragments arranged by size: 5887 642 495 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4091 5149 6014 6312 4091 Size: 1058 865 298 4803 Fragments arranged by size: 4803 1058 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5687 882 Size: 617 4188 2219 Fragments arranged by size: 4188 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4815 493 Size: 711 3611 2702 Fragments arranged by size: 3611 2702 711 BsaBI GATnn'nnATC Cuts at: 2970 7018 2970 Size: 4048 2976 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4831 5213 5848 275 Size: 53 83 186 936 3298 382 635 1451 Fragments arranged by size: 3298 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3510 3727 5457 5855 6129 6276 6687 3510 Size: 217 1730 398 274 147 411 3847 Fragments arranged by size: 3847 1730 411 398 274 217 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 1178 Size: 30 165 30 786 30 5983 Fragments arranged by size: 5983 786 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2986 3106 3354 3820 1186 Size: 850 950 120 248 466 4390 Fragments arranged by size: 4390 950 850 466 248 120 BseYI C'CCAG_C Cuts at: 2270 6227 6723 2270 Size: 3957 496 2571 Fragments arranged by size: 3957 2571 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2118 4115 1163 Size: 573 382 1997 4072 Fragments arranged by size: 4072 1997 573 382 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4087 4474 5235 6263 1540 Size: 275 186 2086 387 761 1028 2301 Fragments arranged by size: 2301 2086 1028 761 387 275 186 BsiHKAI G_wGCw'C Cuts at: 2996 4083 5085 5170 6241 2996 Size: 1087 1002 85 1071 3779 Fragments arranged by size: 3779 1087 1071 1002 85 BsmI GAATG_Cn' Cuts at: 4535 4628 4535 Size: 93 6931 BsmBI CGTCTCn'nnnn_ Cuts at: 3429 4290 3429 Size: 861 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2633 2996 3530 3614 4083 4343 5085 Size: 399 843 363 534 84 469 260 742 Cuts at: 5085 5170 6241 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 843 742 534 469 399 363 260 85 84 BspEI T'CCGG_A Cuts at: 3510 6687 3510 Size: 3177 3847 BspHI T'CATG_A Cuts at: 4913 6643 4913 Size: 1730 5294 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3885 4379 844 Size: 3041 494 3489 Fragments arranged by size: 3489 3041 494 BsrBI CCG'CTC Cuts at: 1394 1823 4015 4911 1394 Size: 429 2192 896 3507 Fragments arranged by size: 3507 2192 896 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5519 5693 65 Size: 1183 4271 174 1396 Fragments arranged by size: 4271 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3254 3626 4274 5668 5855 1700 Size: 110 1444 372 648 1394 187 2869 Fragments arranged by size: 2869 1444 1394 648 372 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7024 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7024 BssSI C'ACGA_G Cuts at: 5084 6096 5084 Size: 1012 6012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2882 794 Size: 2088 4936 BstBI TT'CG_AA Cuts at: 2830 5828 2830 Size: 2998 4026 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7024 BstXI CCAn_nnnn'nTGG Cuts at: 2278 3167 2278 Size: 889 6135 BstYI r'GATC_y Cuts at: 1972 3953 4354 4721 5106 5123 6575 6661 6673 Size: 1981 401 367 385 17 1452 86 12 Cuts at: 6673 7019 1972 Size: 346 1977 Fragments arranged by size: 1981 1977 1452 401 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2338 2338 Size: 7024 BtgI C'CryG_G Cuts at: 513 1065 2805 3597 513 Size: 552 1740 792 3940 Fragments arranged by size: 3940 1740 792 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2396 487 Size: 683 56 1170 5115 Fragments arranged by size: 5115 1170 683 56 BtsI GCAGTG_nn' Cuts at: 2410 2880 2986 3528 4540 5334 5354 2410 Size: 470 106 542 1012 794 20 4080 Fragments arranged by size: 4080 1012 794 542 470 106 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7024 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6989 DraI TTT'AAA Cuts at: 2125 4328 2125 Size: 2203 4821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6031 809 Size: 507 4715 1802 Fragments arranged by size: 4715 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2968 4023 4084 Size: 54 1425 50 275 18 1138 1055 61 Cuts at: 4084 4155 4272 4471 5360 6989 8 Size: 71 117 199 889 1629 43 Fragments arranged by size: 1629 1425 1138 1055 889 275 199 117 71 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4084 4471 1537 Size: 275 2272 387 4090 Fragments arranged by size: 4090 2272 387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2796 3049 3196 3714 4230 4954 1353 Size: 1443 253 147 518 516 724 3423 Fragments arranged by size: 3423 1443 724 518 516 253 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5611 5985 6131 387 Size: 1064 138 4022 374 146 1280 Fragments arranged by size: 4022 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2994 4081 2994 Size: 1087 5937 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2167 2826 2841 2983 3291 3294 Size: 393 410 125 659 15 142 308 3 Cuts at: 3294 4193 5087 5684 6471 6739 1239 Size: 899 894 597 787 268 1524 Fragments arranged by size: 1524 899 894 787 659 597 410 393 308 268 142 125 15 3 EcoNI CCTnn'n_nnAGG Cuts at: 3792 4145 3792 Size: 353 6671 EcoO109I rG'GnC_Cy Cuts at: 2190 2481 2560 3440 3666 3784 3976 2190 Size: 291 79 880 226 118 192 5238 Fragments arranged by size: 5238 880 291 226 192 118 79 EcoRI G'AATT_C Cuts at: 4333 4711 4333 Size: 378 6646 EcoRV GAT'ATC Cuts at: 3931 3931 Size: 7024 FseI GG_CCGG'CC Cuts at: 4278 4278 Size: 7024 FspI TGC'GCA Cuts at: 5530 5530 Size: 7024 HaeII r_GCGC'y Cuts at: 1678 6171 1678 Size: 4493 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2567 2584 2599 3292 Size: 32 135 32 980 15 17 15 693 Cuts at: 3292 3324 3639 3671 3836 3868 3875 3907 3920 Size: 32 315 32 165 32 7 32 13 Cuts at: 3920 3952 4229 4261 5665 5697 5739 5771 1373 Size: 32 277 32 1404 32 42 32 2626 Fragments arranged by size: 2626 1404 980 693 315 277 165 135 42 32 32 32 32 32 32 32 32 32 32 17 15 15 13 7 HincII GTy'rAC Cuts at: 669 1657 1938 4368 4614 669 Size: 988 281 2430 246 3079 Fragments arranged by size: 3079 2430 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7024 HpaI GTT'AAC Cuts at: 4614 4614 Size: 7024 KpnI G_GTAC'C Cuts at: 4349 4349 Size: 7024 MfeI C'AATT_G Cuts at: 4623 4623 Size: 7024 MluI A'CGCG_T Cuts at: 6900 6900 Size: 7024 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6138 6322 1305 Size: 4833 184 2007 Fragments arranged by size: 4833 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2970 4025 10 Size: 54 1425 343 1138 1055 3009 Fragments arranged by size: 3009 1425 1138 1055 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2705 2799 2979 3087 3165 3539 4982 Size: 648 1539 94 180 108 78 374 1443 Cuts at: 4982 5341 5500 518 Size: 359 159 2042 Fragments arranged by size: 2042 1539 1443 648 374 359 180 159 108 94 78 MspA1I CmG'CkG Cuts at: 1606 3389 4440 5117 6265 6510 1606 Size: 1783 1051 677 1148 245 2120 Fragments arranged by size: 2120 1783 1148 1051 677 245 NaeI GCC'GGC Cuts at: 3256 4276 3256 Size: 1020 6004 NcoI C'CATG_G Cuts at: 513 1065 2805 3597 513 Size: 552 1740 792 3940 Fragments arranged by size: 3940 1740 792 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7024 NgoMIV G'CCGG_C Cuts at: 3254 4274 3254 Size: 1020 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7024 NotI GC'GGCC_GC Cuts at: 4471 4471 Size: 7024 NsiI A_TGCA'T Cuts at: 2316 3567 2316 Size: 1251 5773 NspI r_CATG'y Cuts at: 1132 1229 2151 3565 3802 4382 5927 1132 Size: 97 922 1414 237 580 1545 2229 Fragments arranged by size: 2229 1545 1414 922 580 237 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7024 PciI A'CATG_T Cuts at: 5923 5923 Size: 7024 PflMI CCAn_nnn'nTGG Cuts at: 1108 3523 4153 4286 1108 Size: 2415 630 133 3846 Fragments arranged by size: 3846 2415 630 133 PfoI T'CCnGG_A Cuts at: 2183 2513 2183 Size: 330 6694 PmeI GTTT'AAAC Cuts at: 4328 4328 Size: 7024 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2156 2188 2219 2251 5093 5125 6606 6638 2156 Size: 32 31 32 2842 32 1481 32 2542 Fragments arranged by size: 2842 2542 1481 32 32 32 32 31 PpuMI rG'GwC_Cy Cuts at: 2481 2560 3440 3666 3784 3976 2481 Size: 79 880 226 118 192 5529 Fragments arranged by size: 5529 880 226 192 118 79 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7024 PsiI TTA'TAA Cuts at: 4594 4594 Size: 7024 PspOMI G'GGCC_C Cuts at: 3526 3526 Size: 7024 PspXI vC'TCGA_Gb Cuts at: 2173 2173 Size: 7024 PstI C_TGCA'G Cuts at: 830 4376 830 Size: 3546 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7024 PvuII CAG'CTG Cuts at: 3389 3389 Size: 7024 SacI G_AGCT'C Cuts at: 2996 4083 2996 Size: 1087 5937 SalI G'TCGA_C Cuts at: 1936 4366 1936 Size: 2430 4594 SanDI GG'GwC_CC Cuts at: 3666 3666 Size: 7024 SbfI CC_TGCA'GG Cuts at: 4376 4376 Size: 7024 ScaI AGT'ACT Cuts at: 2753 5272 6984 2753 Size: 2519 1712 2793 Fragments arranged by size: 2793 2519 1712 SexAI A'CCwGG_T Cuts at: 2740 3720 2740 Size: 980 6044 SfcI C'TryA_G Cuts at: 826 1045 3073 3812 4372 5507 6188 6379 826 Size: 219 2028 739 560 1135 681 191 1471 Fragments arranged by size: 2028 1471 1135 739 681 560 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7024 SmaI CCC'GGG Cuts at: 1872 4351 1872 Size: 2479 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2173 4106 5128 6029 6291 820 Size: 197 926 230 1933 1022 901 262 1553 Fragments arranged by size: 1933 1553 1022 926 901 262 230 197 SnaBI TAC'GTA Cuts at: 493 4815 493 Size: 4322 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7024 SphI G_CATG'C Cuts at: 1132 3802 4382 1132 Size: 2670 580 3774 Fragments arranged by size: 3774 2670 580 SspI AAT'ATT Cuts at: 5 52 4948 5 Size: 47 4896 2081 Fragments arranged by size: 4896 2081 47 StuI AGG'CCT Cuts at: 1517 2788 3416 1517 Size: 1271 628 5125 Fragments arranged by size: 5125 1271 628 StyI C'CwwG_G Cuts at: 513 1065 2193 2287 2563 2689 2805 2987 3597 Size: 552 1128 94 276 126 116 182 610 Cuts at: 3597 4269 4463 513 Size: 672 194 3074 Fragments arranged by size: 3074 1128 672 610 552 276 194 182 126 116 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3590 5051 5068 5221 5406 5851 325 Size: 1255 2010 1461 17 153 185 445 1498 Fragments arranged by size: 2010 1498 1461 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2751 5270 6982 Size: 274 80 33 51 1443 774 2519 1712 Cuts at: 6982 96 Size: 138 Fragments arranged by size: 2519 1712 1443 774 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2251 2283 3658 3690 6984 7016 2251 Size: 32 1375 32 3294 32 2259 Fragments arranged by size: 3294 2259 1375 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7024 XbaI T'CTAG_A Cuts at: 3398 4360 3398 Size: 962 6062 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 3604 3677 2191 Size: 1413 73 5538 Fragments arranged by size: 5538 1413 73 XhoI C'TCGA_G Cuts at: 1943 2173 1943 Size: 230 6794 XmaI C'CCGG_G Cuts at: 1870 4349 1870 Size: 2479 4545 XmnI GAAnn'nnTTC Cuts at: 2725 5153 2725 Size: 2428 4596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4832 276 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I DraIII FalI FspAI KasI NarI NruI PacI PmlI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI